• 대한임상검사과학회지
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• 제39권2호
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• pp.86-90
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• 2007

Intestinal parasitic infections remain a serious public health problem globally and have usually been associated with human malnutrition. This study was performed to observe the present status of intestinal helminthes infections among the primary school children in Iksan, Korea, during the period from June to August, 2006. A total of 974 fecal samples (male 479, female 495) were collected and examined by formalin-ether sedimentation technique for intestinal helminthes eggs. Of the 974 samples examined, 2 (0.2%) were egg positive for intestinal helminthes, and only eggs of Clonorchis sinensis were observed in the 2 cases. These C. sinensis egg were found in grade 5 (male, 1 case) and grade 6 (female, 1 case), respectively. The egg positive rate of male and female were 0.2%, respectively. Through this survey, I found that the C. sinensis infection is prevalent among primary school children in Iksan, Jeonbuk, and a continuous health education for school children is recommended to prevent the potential infection of C. sinensis.

• 대한임상검사과학회지
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• 제37권3호
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• pp.168-172
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• 2005

The prevalence rate of Enterobius vermicularis eggs was investigated among preschool children located in the area of Iksan, Jeonbuk, during the period from March to July, 2005. Of the 1,082 children examined 78(7.2%) were found to be positive for E. vermicularis egg by the adhesive cellotape anal swab method. The egg positive rate of male and female were 6.7% and 7.8%, respectively. The positive rates range from 5.1% to 8.7% among preschool children, and the highest egg positive rate was observed in the 7 year old age group. Through this survey, it was determined that the E. vermicularis infection is still prevalent among children in Jeonbuk, Korea.

• 대한임상검사과학회지
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• 제50권3호
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• pp.289-296
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• 2018

임상병리학과 학제 단일화에 대한 의견을 교수와 병원근무자들을 대상으로 설문조사 형식으로 조사하여 분석하였다. 2018년 1월 18일부터 25일까지 웹 버전의 설문지가 255명의 대학교수들과 4,000명의 병원근무자들에게 배포되었다. 회수율은 교수들은 79명(30.9%), 병원실무자들은 1,368명(34.2%)이 응답하였다. 임상병리학의 학제 이원화에 대한 인식조사에서는 교수와 병원근무자 모두 4년제로 학제 통합이 필요하다고 조사되었다. 4년제 통합 전제조건은 임상병리 인증평가원 설립과 교육과정의 표준화가 높게 조사되었고, 4년제 통합의 필요조건은 학생정원조정과 교육과정의 표준화가 높게 조사되었다. 4년제 통합방식은 인증평가를 통과한 대학만이 4년제를 운영해야 한다는 의견이 교수와 병원근무자 모두 가장 높게 조사되었다. 4년제 통합시 대학정원 적정인원은 40명 미만이 가장 높게 조사되었다. 결론적으로 교수들과 병원실무자들은 의료환경의 급격한 변화에 부응하는 전문성을 가진 임상병리사를 배출하기 위해서는 임상병리학과 학제의 4년제 통합이 필수적이고, 이를 위해 임상병리인증평가원 설립, 교육과정의 표준화, 학생정원조정 이 전제되어야 한다고 생각하고 있음을 보여준다.

• 대한의생명과학회지
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• 제24권3호
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• pp.157-167
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• 2018

The concept of gene therapy is to treat a disease by transferring therapeutic nucleic acids to a patient's cells. It took several decades from the basic theoretical proposal of gene therapy to the current promising treatment option for some important human diseases. The encountered adverse effects in the early clinical studies boosted the development of sophisticated vectors and elaborate clinical designs. The gene therapy is now considered to have the potential to cure many diseases that are incurable with conventional medications. By the end of 2017, about 2,600 clinical trials of gene therapy have been performed or are ongoing for a variety of diseases such as cancers, monogenic diseases, cardiovascular diseases and neurological diseases etc. Here, we present a brief introduction of technical achievement in relation to gene therapy development, and a review of the current status of global gene therapy clinical development.

• Journal of Veterinary Science
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• 제21권6호
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• pp.81.1-81.10
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• 2020

Background: Although previous in vivo studies explored urinary microRNA (miRNA), there is no agreement on nephrotoxicity-specific miRNA biomarkers. Objectives: In this study, we assessed whether urinary miRNAs could be employed as biomarkers for nephrotoxicity. Methods: For this, literature-based candidate miRNAs were identified by reviewing the previous studies. Female Sprague-Dawley rats received subcutaneous injections of a single dose or repeated doses (3 consecutive days) of gentamicin (GEN; 137 or 412 mg/kg). The expression of miRNAs was analyzed by real-time reverse transcription-polymerase chain reaction in 16 h pooled urine from GEN-treated rats. Results: GEN-induced acute kidney injury was confirmed by the presence of tubular necrosis. We identified let-7g-5p, miR-21-3p, 26b-3p, 192-5p, and 378a-3p significantly upregulated in the urine of GEN-treated rats with the appearance of the necrosis in proximal tubules. Specifically, miR-26-3p, 192-5p, and 378a-3p with highly expressed levels in urine of rats with GEN-induced acute tubular injury were considered to have sensitivities comparable to clinical biomarkers, such as blood urea nitrogen, serum creatinine, and urinary kidney injury molecule protein. Conclusions: These results indicated the potential involvement of urinary miRNAs in chemical-induced nephrotoxicity, suggesting that certain miRNAs could serve as biomarkers for acute nephrotoxicity.

• 대한임상검사과학회지
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• 제54권2호
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• pp.163-166
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• 2022

Acquired hemophilia A (AHA) is an uncommon autoimmune bleeding disorder in which autoantibodies that affect the functions of factor VIII (FVIII) are present in the blood. The initial diagnosis of AHA is difficult as the presentations of AHA differ from those of congenital hemophilia A. Moreover, the treatment of AHA is more complex due to the presence of autoantibodies against FVIII. Here, we present a case report of postpartum AHA, to increase the perception and knowledge regarding the recognition and management of such cases. We present a young female with the chief complaint of vaginal bleeding and upper arm ecchymosis. Laboratory results exhibited isolated prolonged activated partial thromboplastin time (APTT) and FVIII inhibitors. The patient was treated with corticosteroids, FVIII concentrates, and a bypassing agent. In conclusion, unexplained postpartum bleeding, unmanageable with basic hemostatic measures, should lead to clinical suspicion of an acquired bleeding disease.

• 대한임상검사과학회지
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• 제56권2호
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• pp.105-114
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• 2024

임상검사실은 질관리 시스템(quality management system)을 구축하여 일정 수준 이상의 질 확보를 위해 노력하여야 한다. 그러나 검사실 환경은 매우 복잡하여 단일 정도관리 절차로는 다양한 유형의 오류를 감지하는데 충분하지 않을 수 있다. 환자 기반 실시간 정도관리(patient-based real-time quality control, PBRTQC)는 테스트 과정을 모니터링하기 위한 검사실 도구로써 Bull's 알고리즘, 정상치 평균, 이동 중앙값, 이동평균, 지수가중이동평균과 같은 알고리즘이 활용되고 있다. PBRTQC는 저렴한 비용, 교환 가능성, 지속적인 실시간 성능 모니터링, 분석 전 오류에 대한 민감도 등 기존 정도관리에 비해 많은 이점이 있다. 그러나 PBRTQC는 통계 알고리즘의 선택, 적절한 규칙과 프로토콜의 설계, 성능검증 등을 고려해야하므로 구현하기가 쉽지만은 않다. 본 리뷰에서는 PBRTQC의 기본 개념과 방법 및 절차에 대해 설명하였으며, 이를 통해 환자 기반 정도관리 시스템 구현을 위한 지침을 제시하고자 하였다. 이에 기존의 내부정도관리의 성능이 제한적일 경우 PBRTQC 절차를 병용하는 것을 제안하고자 한다. 본 리뷰에서는 임상적 평가는 배제되었으며, 향후 이에 대한 평가가 요구된다.

• 대한임상검사과학회지
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• 제36권2호
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• pp.210-214
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• 2004

This study was carried out to predict the value of serum ${\beta}$ subunit of humans chorionic gonadotropin(${\beta}$- hCG) in early pregnancy viability. This was performed among 85 women in vitro fertilization and embryo transfer(IVF-ET). The serum ${\beta}$-hCG levels were established for 30 normal singleton pregnancies, 10 twin and triplet pregnancies, 10 preclinical abortions, 10 clinical abortions, 20 biochemical abortions and 5 ectopic pregnancies. In comparison to normal singleton pregnancies, multiple pregnancies showed higher ${\beta}$-hCG. But clinical abortions, preclinical abortions and ectopic pregnancies showed lower ${\beta}$-hCG levels than singleton pregnancies. In conclusion, if we predict the value of serum ${\beta}$-hCG of variable early pregnancies and analyze it, we could predict the dilution protocol. Also, it can be useful in other ways.

• 대한의생명과학회지
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• 제18권3호
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• pp.260-267
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• 2012

Obesity is associated with cardiovascular risk factors, such as dyslipidemia, hypertension and diabetes. However the presence of the obesity related deranged metabolic profiles varies widely among obese individuals. These individuals, known as 'metabolically healthy obese phenotype (MHO)', despite having excessive body fatness, display favorable metabolic profiles characterized by insulin sensitivity, no hypertension, as well as less dyslipidemia, less inflammation. The purpose of this study was to compare cardiac characterization and clinical profile of MHO and Non-MHO (nonmetabolically healthy obese) subjects in men. We measured treadmill exercise capacity (METs) and maximum blood pressure (BP) in 210 subjects through a medical checkup at J General Hospital. Metabolic syndrome was defined according to the modified Adult Treatment Panel III definition criteria. Both MHO and Non-MHO subjects showed statistically significant changes in the left ventricular mass index (P<.001, P<.01, respectively), A-velocity (P<.01, P<.001, respectively), E/A ratio (P<.01, P<.001, respectively), E'-velocity (P<.001, P<.001, respectively), HOMA-IR (P<.01, P<.001, respectively) and maximum systolic BP (P<.01, respectively) compared with the MH-NO (metabolically healthy non obese) subjects. In conclusion, MHO participants were at increased risk of cardiovascular disease and partly metabolic disorder.

• 대한임상검사과학회지
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• 제55권3호
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• pp.203-212
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• 2023

코로나19가 창궐한 후 임상병리사(medical technologists, MT)가 병원에서 PCR 검사를 수행하고 있을 뿐만 아니라 차세대 염기서열분석(next-generation sequencing, NGS)도 수행한다는 사실을 일반적으로 알고 있다. 하지만, 많은 사람들은 유전자검사 분야에 대한 임상병리사의 업무로 의료법에 명시되어 있지 않다는 사실을 인지하지 못하고 있는 실태이다(MT 72.5%, N=200; 학생 62.8%, N=123). 이러한 이유로, NGS에 대한 임상병리사의 업무 적절성을 검증하기 위해서 온라인 설문지를 작성하도록 설계하여 설문조사 결과를 분석하였다. NGS가 포함된 유전분야에 대한 임상병리사의 업무범위에 대한 법제화가 필요하다(MT 99.5%, N=200, 학생 86.8%, N=123)고 나타났다. 그리고 임상병리사 국가면허시험 문제은행에 세포유전학 및 분자유전학 관련 임상유전학 문항이 모두 포함돼야 한다(MT 97.5%, N=200; 학생 72.3%, N=123)고 조사되었다. 이러한 조사를 바탕으로 임상병리학 분야의 발전을 위해 임상병리사와 학생 모두를 위한 유전자 교육 및 법제화를 위해 대한임상병리사협회는 교수협의회와 적극 협력할 필요가 있겠다.