• Applied Biological Chemistry
• /
• v.50 no.3
• /
• pp.238-243
• /
• 2007

The contents of limonin, nomilin and limonin glucoside in different tissues of dangyuja (Citrus spp.) were measured during fruit growth and maturation by liquid chromatography-mass spectrometry(LC-MS). Both nomilin and limonin contents increased from June, peaked in October and in December, respectively, and then decreased afterwards. In contrast, the content of limonin glucoside increased from October and remained at high levels throughout the maturation. The contents of limonin, nomilin and limonin glucoside in peel decreased gradually throughout the growth and the trends of changes were similar to each other. However, all three limonoid contents in juice sac increased dramatically at the end of fruit maturation. The highest concentration of limonin and nomilin was observed in seed, followed by segment membrane, whereas the highest limonin glucoside concentration was observed in segment membrane, followed by juice sac.

• Journal of The Korean Society of Integrative Medicine
• /
• v.11 no.3
• /
• pp.159-169
• /
• 2023

Purpose : Though other Citrus spp. have reported their anti-inflammatory and antioxidative activities in previous studies, the biological activity of Kiyomi (Citrus unshiu × C. sinensis) has not been reported yet. Therefore, this study attempted to analyze the anti-inflammatory mechanisms of Kiyomi leaf ethanol extract (KLEE) in lipopolysaccharide (LPS) stimulated RAW 264.7 cells. Methods : The cytotoxic effect of KLEE in RAW 264.7 cells was determined by WST-1 assay. Bacterial endotoxin, the concentration of nitric oxide (NO) was analyzed by the Griess reaction. In addition, Western blot analysis was applied to measure the protein expression level of inducible NO synthase (iNOS). The phosphorylated status of the critical inflammatory transcription factor, nuclear factor (NF)-𝜅B, and its upstream signaling molecules, phosphoinositide 3-kinase (PI3K)/Akt as well as mitogen-activated protein kinases (MAPKs), were also measured by Western blot analysis. Results : KLEE was not cytotoxic up to a concentration of 200 ㎍/㎖, and protein expression levels of iNOS and cyclooxygenase (COX)-2, enzymes that counteract NO and prostaglandin (PG) E2 production, were inhibited by KLEE treatment. The phosphorylated status of PI3K/Akt as well as MAPKs including extracellular regulated kinase (ERK), c-jun NH2kinase (JNK), and p38, were significantly attenuated by KLEE treatment in LPS stimulated RAW 264.7 cells. Moreover, one of phase II enzymes, heme oxygenase (HO)-1 which has known for its anti-inflammatory capacity, was strongly induced by KLEE treatment. Conclusion : Consequently, KLEE treatment significantly attenuated the production of NO as well as the expression levels of iNOS and COX-2 in LPS-stimulated RAW 264.7 cells. The inflammatory transcription factor, NF-𝜅B, as well as its upstream signaling molecules, PI3K/Akt and MAPKs, were also diminished by KLEE treatment with statistical significance in LPS-stimulated RAW 264.7 cells. These results suggest that KLEE might be a promising candidate for the attenuation of inflammatory disorders.

• Research in Plant Disease
• /
• v.30 no.1
• /
• pp.78-81
• /
• 2024

This study aims to isolate and identify the fungal pathogen responsible for gummosis disease affecting Thanh Tra pomelo in Vietnam. Through molecular identification utilizing primer pairs ITS5 and ITS4, the analysis pinpointed Lasiodiplodia theobromae as the specific fungal pathogen. Notably, the fungal colonies exhibited vigorous growth on potato dextrose agar. Initially, these colonies appeared whitish-grey, transforming into a black-grey hue within 5-7 days at a temperature of 30℃. According to previous reports, Phytophthora spp. was the most common pathogenic genus causing gummosis on Thanh Tra pomelo in Vietnam. To our knowledge, this is the first report on L. theobromae causing gummosis on Thanh Tra pomelo in Vietnam.

• Korean Journal of Plant Tissue Culture
• /
• v.21 no.3
• /
• pp.145-150
• /
• 1994

This study was conducted to determine the effects of 3 cytokinins (BA,2iP and kinetin) and their concentrations (0, 0.1, 0.5, 1.0, and 2.0 mg/L) on multiple shoot production of Citrus spp. 'Sambokam' and 'Byungkyool' by nodal culture. Nodal explants were obtained from in vitro germinated seedlings of both cultivars. 'Sambokam' produced more multiple shoots than did 'Byungkyool' by nodal culture. Among the 3 cytokinins tested in this study BA supplemented in semi-solid MS basal medium was the most effective stimulator for multiple shoot production, and an optimal concentration was determined to be 1.0 mg/L. Shoot elongation and root formation were inhibited by increasing cytokinin concentration, regardless of cytokinin types. BA at 1.0 mg/L produced the most multiple shoots and the highest number of leaves in 'Sambokam', whereas any cytokinin and concentration studied in this experiment did not affect any scored variables such as shoot and leaf numbers, etc. in 'Byungkyool'.

• Korean journal of applied entomology
• /
• v.42 no.3
• /
• pp.269-277
• /
• 2003

Some species of true bugs have become serious problems in rice, upland crops, and tree fruits. It would be meaningful to understand research status by reviewing articles on those true bugs in Korea. Articles on those bugs published in several scientific Korean journals were reviewed, except articles on true bugs on rice plants; CD Part 1 included classification and morphological studies on eggs and larvae of Piesma spp., on external genitalia of Gonopsis affinis, and on spermathecae of some Podopinae and Asopinae species. $\circled2$ Development and growth analysis of Piesma sp., P. maculata, and 2 species of Coreidae were reviewed in part 2. $\circled3$ In part 3 we reviewed with major pest bug species on soybean, sweet persimmon, yuzu, citrus, chrysanthemum, and Cynanchum wilfordii, and insect fauna in mountain areas. $\circled4$ In part 4, damage levels in soybean, sweet persimmon, yuzu, grapes were reviewed. $\circled5$ ID In part 5 we reviewed seasonal occurrence patterns of Halyomorpha halys, Plautia stali, Riptortus clavatus in sweet persimmon orchards, of some species in soybean fields, of Nysius plebejus on chrysanthemum, and of Tropidothorax cruciger on Cynanchum wilfordii. $\circled6$ Chemical control methods in a sweet persimmon orchard, in grapevine yards, in a soybean field, and in a chrythansemum field were introduced in part 6. Some laboratory bioassay on insecticides against R. clavatus were mentioned, too. $\circled7$ Finally in part 7, researches on transmission by Halyomorpha halys and Cyrtopeltis tenuis of micoplasma-like organism which is a pathogen of paulownia withces' -broom to Catharanthus roseus were reviewed.

• Asian-Australasian Journal of Animal Sciences
• /
• v.16 no.11
• /
• pp.1661-1665
• /
• 2003

A feeding trial was conducted for 60 days to study the utilization of fruits processing wastes as feed ingredient in the diet of Labeo rohita fingerlings. One hundred and sixty fingerlings (av. body weight, $1.65g{\pm}0.03$) were equally distributed in four experimental groups having 4 replicates each. Four different experimental diets were prepared by replacing wheat flour and rice bran with either orange (T2) (Cirtus qurantium), pineapple (T3) (Ananas spp. and Pseudananas spp.) or sweet lime (T4) (Citrus sinensis) wastes to the basal diet along with the control (T1, without any fruit wastes) keeping the CP level at around 40%. The water quality parameters like DO, $CO_2$, pH, total alkalinity, total hardness, ammonia and water temperature were recorded within the optimum range. The diet containing 25% pineapple wastes (T3) showed significantly higher growth in terms of SGR (1.50), FCR (2.09) and PER (1.19) than the other groups. However, growth of T4 and T2 groups were not significantly different than the control group (T1). Protease activity (17.17 unit/mg protein), protein digestibility (91.57%) and carbohydrate digestibility (41.62%) were not significantly different among the different groups. Survival of the fingerlings were not significantly different among the experimental groups. It concludes that waste of orange, pineapple and sweet lime can be used at 25% level as a substitute of wheat flour and rice bran in the diet of Labeo rohita.

• Korean Journal of Weed Science
• /
• v.30 no.3
• /
• pp.191-198
• /
• 2010

The objective of this study was to find herbicidal compounds from seven different plant essential oils such as amyris (Amyris balsamifera), cajuput (Melaleuca cajeputi), geranium (Pelargonium graveolens), lavender (Lavendula spp.), mandarin (Citrus reticulata), pine (Pinus spp.) and rosemary (Rosmarius officinale), and determine their herbicidal activities. The in vitro herbicidal activity of cajuput essential oil was the highest among six essential oils ($GR_{50}$ value, $425{\mu}g\;g^{-1}$) and major chemical components in cajuput essential oil were eucalyptol (37.2%), ${\alpha}$-terpineol (11.6%), benzaldehyde (5.2%), linalool (4.1%), ${\alpha}$-pinene (2.5%) and ${\beta}$-pinene (2.4%), and their $GR_{50}$ values were 2,731, 500, 50, 372, 4,363, and $4,671{\mu}g\;g^{-1}$, respectively. Soil application of cajuput essential oil and benzaldehyde did not show any herbicidal activity at 80 kg $ha^{-1}$. When cajuput essential oil was applied to foliar at 80 kg $ha^{-1}$, narrow-leaved plants such as sorghum (Sorghum bicolar), barnyardgrass (Echinochloa crus-galli), and southern crabgrass (Digitaria ciliaris) were killed 100%, however, broad-leaved plants indian jointvetch (Aeschynomeme indica), velvet leaf (Abutilon theophrasti), cocklebur (Xanthium strumarium), Japanese morningglory (Calystegia japonica) were not killed, indicating the cajuput essential oil was effective to control narrow-leaved plants. Herbicidal activities of benzaldehyde at 80 kg $ha^{-1}$, to those plants were 20, 60 and 95%, respectively. Overall data showed that the herbicidal activity of cajuput essential oil was in part due to benzaldehyde.

• Journal of Life Science
• /
• v.20 no.2
• /
• pp.190-196
• /
• 2010

Nowadays many people use antibiotics to protect processed foods from many pathogenic bacteria. The abuse of antibiotics, however, can run the risk of creating resistant forms of bacterium. Our study focus is on making new substances that can not only replace antibiotics but also be friendly to the environment. In our experiments, we used fermented citrus fruit, soil microbes and coenzyme Q10 as probiotics and prebiotics. Chickens in the experimental group were fed these substances via oral route while those in the control group were not. After specific time periods, blood and feces samples were collected to test for Salmonella spp.. It is interesting that fermented citrus fruit was the most effective in suppressing this bacterium. Furthermore, dissection of the experiment group chickens shows that their livers did not change to a yellow color, in contrast to the control group. The results confirmed our proposal that the chickens fed with these materials can be protected from infection by Salmonella and other pathogens. These probiotics and prebiotics are highly practical because they are natural substances that can be easily recycled in the environment. It can also be used as an animal feed ingredient because of its safety.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.46 no.3
• /
• pp.389-393
• /
• 2017

This study investigated the effects of Sinetrol-XPur (polyphenolic Citrus spp. and Paullinia cupana Kunth dry extract) on lipolysis using leptin-deficient obese (ob/ob) mice. Obese mice were treated with two different doses, 100 mg/kg body weight (B.W.) and 300 mg/kg B.W. in each AIN93G supplement, for 7 weeks. Body weight gain in obese mice treated with both low and high doses of Sinetrol-XPur was reduced compared with control obese mice. Abdominal and visceral adipose tissue weight of mice were reduced in high dose supplemented groups. Epididymal adipose tissue weight was reduced in both low and high dose supplemented groups by 18.27% and 41.05%, respectively. Phosphodiesterase 3B (PDE3B) mRNA levels decreased upon Sinetrol supplementation in adipose tissue of ob/ob mice, whereas A kinase anchor protein 1 (AKAP1), adipose triglyceride lipase (ATGL), and perilipin (PLIN) mRNA levels increased. These results suggest that Sinetrol-XPur supplementation partially stimulates lipolysis through reduction of PDE3B and induction of AKAP1, ATGL, and/or PLIN gene expression, resulting in reduced body and white adipose tissue weight.

• Proceedings of the Korean Society of Applied Pharmacology
• /
• 2003.11a
• /
• pp.85-85
• /
• 2003

This study has been undertaken to increase availability of native camellia in Jeonnam as a medicinal resource and to isolate the effective components from them. Multidrug resistance(MDR) by tumor cells is a major obstacle to successful cancer chemotherapy. We report that the crude extracts of camellia flowers, leaves has a chemosensitizing effect that can reverse Pgp-mediated MDR by increasing the intracellular accumulation of drugs. The cytotoxic and chemosensitizing effects of MeOH extract from 12 spp. citrus fruits on the AML-2/D100 were determined using MTT assay. Chemosensitizing effects was screened in the presence of vincristine, a good substrate of Pgp. IC$\sub$50/ for extracts in AML-2/WT was found to be 65∼350$\mu\textrm{g}$/$m\ell$ whereas the range of its mean IC$\sub$50/ value in Pgp-overexpressing cells (AML-2/Dl00) in the presence of vincristine was 90∼400$\mu\textrm{g}$/$m\ell$. Of the extracts tested, mature leaf extract displayed the most potent chemosensitizing effect［IC$\sub$50/;100 $\mu\textrm{g}$/$m\ell$, CR;1.06, RF;2.97 in the presence of VCR］. This indicates that the toxicity (IC$\sub$50/;288.89$\mu\textrm{g}$/$m\ell$) of mature leaf extract is minimal at concentrations required for a complete reversal of the drug resistance. Also, this result indicates that crude extracts of camellia mature leaves would contain some principles which have chemosensitizing activity.