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http://dx.doi.org/10.5352/JLS.2010.20.2.190

Prevention of Salmonella Infection in Layer Hen Fed with Microbial Fermented Citrus Shell  

Kang, Tae-Yoon (Animal Biotechnology, College of Applied Biosciences, Jeju National University)
Kang, Syung-Tae (Animal Biotechnology, College of Applied Biosciences, Jeju National University)
Ihn, Young-Ho (The research Institute for Biotechnology in CT&D Co.)
Lee, Yang-Ho (The research Institute for Biotechnology in CT&D Co.)
Cho, Don-Young (Global C&B Co. Ltd.)
Lee, Sung-Jin (Department of Animal Biotechnology, Kangwon National University)
Son, Won-Geun (Department of Veterinary Sciences, College of Veterinary Sciences, Jeju National University)
Heo, Moon-Soo (Faculty of Marine Biomedical Sciences, College of Ocean Sciences, Jeju National University)
Jeong, Dong-Kee (Animal Biotechnology, College of Applied Biosciences, Jeju National University)
Publication Information
Journal of Life Science / v.20, no.2, 2010 , pp. 190-196 More about this Journal
Abstract
Nowadays many people use antibiotics to protect processed foods from many pathogenic bacteria. The abuse of antibiotics, however, can run the risk of creating resistant forms of bacterium. Our study focus is on making new substances that can not only replace antibiotics but also be friendly to the environment. In our experiments, we used fermented citrus fruit, soil microbes and coenzyme Q10 as probiotics and prebiotics. Chickens in the experimental group were fed these substances via oral route while those in the control group were not. After specific time periods, blood and feces samples were collected to test for Salmonella spp.. It is interesting that fermented citrus fruit was the most effective in suppressing this bacterium. Furthermore, dissection of the experiment group chickens shows that their livers did not change to a yellow color, in contrast to the control group. The results confirmed our proposal that the chickens fed with these materials can be protected from infection by Salmonella and other pathogens. These probiotics and prebiotics are highly practical because they are natural substances that can be easily recycled in the environment. It can also be used as an animal feed ingredient because of its safety.
Keywords
Antibiotics; citrus fruit; soil microbe; coenzyme Q10; food safety;
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1 Kim, Y. K., Y. B. Kim, and D. J. Kim. 2006. Patent for the method of feed additive and production using citrus component. No. 10-0688441-0000.
2 Kim, J. H. 2008. The effect of anti-bacterial against poultry bacteria using Saltose in mixed probiotic from bacilus. Master thesis of Korea university.
3 Mandalari, G., R. N. Bennett, G. Bisignano, D. Trombetta, A. Saija, C. B. Faulds, M. J. Gasson, and A. Narbad. 2007. Antimicrobial activity of flavonoids extracted from bergamot (Citrus bergamia Risso) peel, a byproduct of the essen tial oil industry. J. Appl. Microbiol. 103, 2056-2064.   DOI
4 Moon, Y. G., K. J. Lee, K. Y. Kim, C. B. Song, Y. J. Jun, and M. S. Hue. 2006. Characterization of waste from citrus fermented product using probiotics. J. of Korean Microbiology and Biotechnology 34, 158-165.
5 Park, S. H. Y. A. Kim, D. K. Lee, S. J. Lee, M. J. Chung, B. Y. Kang, K. J. Kim, and N. J. Ha. 2007. Antibacterial activity and macrophage activation of lactic acid bacteria. The Korean Journal of Environmental Toxicology 22, 287-297.   DOI   ScienceOn
6 Sakamoto, S., I. Shooro, and M. Arai. 1982. Reduced cellulose as substrate of cellulases. J. Ferment. Technol. 60, 327.
7 Roza, J. M., Z. Xian-Liu, and N. Guthrie. 2007. Effect of citrus flavonoids and tocotrienols on serum cholesterol levels in hypercholesterolemic subjects. Altern. Ther. Health Med. 13, 44-48.
8 Yang, S. J., Y. H. Moon, B. W. Lee, S. M. Ko, and T. I. Yang. 2007. The research on the citrus byproducts production actual conditions, the conditions of utilizing feeds and the citrus byproducts ingredients analysis. pp. 17-72. Research report for the development of functional poultry production technology using citrus.
9 Ye, C. Q., K. Folkers, H. Tamagawa, and C. Pfeiffer. 1988. A modified determination of coenzyme Q10 in human blood and CoQ10 blood levels in diverse patients with allergies. Biofactors 1, 303-306.
10 Folkers, K., R. Brown, W. V. Judy, and M. Morita. 1993. Survival of cancer patients on therapy with coenzyme Q10. Biochem. Biophys. Res. Commun. 192, 241-245.   DOI
11 Hoffmann, P. C., H. W. Kunz, W. Schmid, and M. Siess. 1994. The determination of the level and redox quotient of ubiquinone50 (Coenzyme Q10) in guinea pig heart in vivo. Biochem. Z. 339, 548-558.
12 Folkers, K., M. Morita, and J. Jr McRee. 1993. The activities of coenzyme Q10 and vitamin B6 for immune responses. Biochem. Biophys. Res. Commun. 193, 88-92.   DOI
13 Gazdik, F., A. Gvozdjakova, R. Nadvornikova, L. Repicka, E. Jahnova, J. Kucharska, M. R. Pijak, and K. Gazdikova. 2002. Decreased levels of coenzyme Q(10) in patients with bronchial asthma. Allergy 57, 811-814.   DOI
14 Geng, A. L., Y. M. Guo, and Y. Yang. 2004. Reduction of ascites mortality in broilers by coenzyme Q10. Poult. Sci. 83, 1587-1593.   DOI
15 Hoppe, U., J. Bergemann, W. Diembeck, J. Ennen, S. Gohla, I. Harris, J. Jacob, J. Kielholz, W. Mei, D. Pollet, D. Schachtschabel, G. Sauermann, V. Schreiner, F. Stab, and F. Steckel. 1999. Coenzyme Q10, a cutaneous antioxidant and energizer. Biofactors 9, 371-378.   DOI
16 Jeong, J. Y., N. S. Ahn, J. S. Park, Y. H. Cho, J. W. Hwang, J. R. Park, S. J. Kim, Y. G. Lee, Y. H. Jeong, J. H. Jeong, S. H. Lee, Y. S. Park, B. G. Park, B. S. Kim, S. G. Kim, S. B. Lee, G. H. Lee, K. S. Kang, and Y. S. Lee. 2006. Research of immunological improvement using new material (immuneforte). J. Fd. Hyg. Safety 21, 113-117.
17 Johann, S., V. Lopes de Oliveira, M. G. Pizzolatti, J. Schripsema, R. Braz-Filho, A. Branco, and A. Smânia. 2007. Antimicrobial activity of wax and hexane extracts from Citrus spp. peels. Mem. Inst. Oswaldo. Cruz. 102, 681-685.   DOI   ScienceOn
18 Kim, H. S., J. S. Hyun, J. Kim, H. P. Ha, and D. S. Yoo. 1998. Identification of enzyme Characteristic of useful fungi isolation from Korea tradition malt. Korean J. of Industrial Microbiology 26, 456-464.
19 De Luca, C., I. Deeva, E. Mikhal'Chik, and L. Korkina. 2007. Beneficial effects of pro-/antioxidant-based nutraceuticals in the skin rejuvenation techniques. Cell Mol. Biol. 53, 94-101.
20 Coulson, N. M. and R. W. Titball. 1993. Expression of Bacillus anthracis Protective Antigen in Salmonella typhimurium. NATO ASI series. Series A, Life Sciences 245, 419.