• Bulletin of the Korean Chemical Society
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• v.27 no.10
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• pp.1618-1622
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• 2006

Bacteriophage T7 gp4A' is a ring-shaped hexameric DNA helicase that catalyzes duplex DNA unwinding using dTTP hydrolysis as an energy source. To investigate the effect of single-stranded DNA (ssDNA) on the kinetic pathway of dTTP hydrolysis by the T7 DNA helicase complexed with ssDNA, we have first determined optimal concentration of long circular M13 single-stranded DNA and pre-incubation time in the absence of $Mg^{2+}$ which is necessary for the helicase-ssDNA complex formation. Steady state dTTP hydrolysis in the absence of $Mg^{2+}$ by the helicase-ssDNA complex provided $k_{cat}$ of $8.5\;{\times}\;10^{-3}\;sec^{-1}$. Pre-steady state kinetics of the dTTP hydrolysis by the pre-assembled hexameric helicase was monitored by using the rapid chemical quench-flow technique both in the presence and absence of M13 ssDNA. Pre-steady state dTTP hydrolysis showed distinct burst kinetics in both cases, indicating that product release step is slower than dTTP hydrolysis step. Pre-steady state burst rates were similar both in the presence and absence of ssDNA, while steady state dTTP hydrolysis rate in the presence of ssDNA was much faster than in the absence of ssDNA. These results suggest that single-stranded DNA stimulates dTTP hydrolysis reaction by T7 helicase by enhancing the rate of product release step.

• Transactions of the Korean Society of Automotive Engineers
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• v.22 no.1
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• pp.117-125
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• 2014

The temperature distribution of steady state rolling tires with detailed tread blocks is numerically predicted using the three dimensional full patterned tire model. A three dimensional periodic patterned tire model is constructed by copying 1-sector mesh in the circumferential direction. Using the static tire contact analysis, the strain cycles during one revolution are approximated with the strains at Guassian points of the elements which are sector-wise repeated within the same circular ring of elements, by neglecting the tire rolling effect. Based upon the multi-axial fatigue theory, the maximum principal strain is used to represent the combined effect of six strain components on the hysteretic loss. In the following, the deformation due to the inflation and vertical load is calculated using ABAQUS. Then heat generation rate in each element is calculated using an in-house code. Lastly, temperature distribution is calculated using ABAQUS again. Through the numerical experiments, the validity of the proposed prediction method is examined by comparing with the experiment and the temperature distribution of a patterned tire model is compared with those of the main-grooved simple tire model.

• Proceedings of KOSOMES biannual meeting
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• 2003.05a
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• pp.125-134
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• 2003

This paper describes on the design of Passive-type Radar Reflector for small Ship (PRR-S) based on the newly revised 2000 SOLAS regulations. The design idea, adopted in the study, is to hold PRR-S in the proper ‘catch rain’ position to avoid fluctuations of Radar Cross Section (RCS) due to ship's heeling. The PRR-S consists of octahedral-type radar reflector with circular plates and three-axis gimbaled stabilizer with weight on the bottom of outer gimbal ring. Performance test for the PRR is carried out in an anechoic chamber. The test results show that the reflected radar signal from PRR-S is more uniformly distributed than the reference model (Davis Echomaster).

• Proceedings of the Korean Geotechical Society Conference
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• 2005.03a
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• pp.401-408
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• 2005

Recently shield TBM tunnellings are being applied to subway construction in Korean cities. Generally these kinds of tunnellings have the problems in the stability of ground such as subsidence because urban subway is constructed in the shallow depth. A sinkhole occurred on the road just above the tunnel during tunneling in Kwangju, so a survey for upper layer of the tunnel was needed. But conventional Ground Probing Radar can't be applicable due to the presence of steel-mesh screen in the shield segment, so no existent geophysical method is applicable in this site. Because the outer surface of each shield segment is electrically insulated, dipole-dipole resistivity method which is popular in engineering site investigation, was tried to this survey for the first time. Specially manufactured flexible ring-type electrodes were installed into the grouting holes at an interval of 2.4 m on the ceiling. The K-Ohm II system which has been developed by KIGAM and tested successfully in many sites, was used in this site. The system consists of 1000Volt-1Ampere constant-current transmitter, optically isolated 24 bit sigma-delta A/D conversion receiver - maximum 12 channel simultaneous measurements, and graphical automatic acquisition software for easy data quality check in real time. Borehole camera logging with circular white LED lighting was also done to investigate the state of the layer. Measured resistivity data lack of some stations due to failing opening lids of holes, shows general high-low trend well. The dipole-dipole resistivity inversion results discriminate (1) one approximately 4 meter diameter cavity (grouted but incompletely hardened, so low resistivity - less than $30{\Omega}m$), (2) weak zone (100-200${\Omega}m$), and (3) hard zone (high resistivity - more than 1000${\Omega}m$) very well for the distance of 320 meters. The 2-D inversion neglects slight absolute 3-D effect, but we can get satisfactory and useful information. Acquired resistivity section and video tapes by borehole camera logging will be reserved and reused if some problem occurs in this site in the future.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.22 no.4
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• pp.450-467
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• 1998

The characteristics of the unstable impinging circular jet is investigated based on the frequency characteristics and the sound field of the impinging-tones. Two symmetric modes S1 and S2, associated with low frequency and high frequency respectively, and one helical mode H have been observed. At low speed the S2 mode is dominant and switched by the S1 mode as the speed increases. When the jet speed is high the S1 mode is very active over the impinging distance from half the nozzle diameter to its ten times, while the S2 mode occurs at shorter distance corresponding to stage 2 and 3. The helical mode H seems unstable, likely to be influenced much by the experimental environment, and occurs at relatively high speed with almost the same frequency characteristics as the S2 mode. By estimating the convection speed of the unstable jet, it is found that the ratio of the convection speed to the jet speed decreases with both Strouhal number and Reynolds number and the speed of S2 mode is faster than the Si mode. When the present experimental results are compared with the previous investigations performed for the hole tone and the impinging tone with a small plate, the S1 mode is found to be associated with the ring vortex of large diameter with low speed, but the S2 mode with the vortex of small diameter with high speed. In addition, the frequency is found to be influenced by the nozzle configuration but the characteristics is almost the same. From the impinging distance and frequency range, it can be deduced that S1 mode is related with the jet column mode and S2 mode with the shear mode.

• Korean Journal Plant Pathology
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• v.13 no.4
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• pp.195-199
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• 1997

Anthracnose symptoms were observed on the commercially cultivating ultivating cyclamen in Chonbuk province in 1995. The symptoms of infected flowers were small, circular and dark brown ring-spots or water-soaked lesions and gradually changed to lack blight. Mycelial colony of the isolates was light or whitish gray to dark gray on potato dextrose agar. Conidia were straight cylindrical and obtuse at the apex and measured 8.3~15.0${\times}$2.5~6.3 ${\mu}m$ in size. Appressoria were brown to dark brown and clavate, but most of them were irregular. Acervuli on lesions were brown, rounded and measured 50~140${\times}$32.5~90 ${\mu}m$ in size. The optimum temperatures for mycelial growth and conidial sporulation were ranged from 25 to 30$^{\circ}C$. Thus, based on mycological characteristics of the fungus, the causal agent of cyclamen anthracnose was identified as Colletotrichum gloeosporioides Penz.

• Journal of Microbiology and Biotechnology
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• v.24 no.4
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• pp.522-533
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• 2014

Bionanotechnology has revolutionized nanomaterial synthesis by providing a green synthetic platform using biological systems. Among such biological systems, microalgae have tremendous potential to take up metal ions and produce nanoparticles by a detoxification process. The present study explores the intracellular and extracellular biogenic syntheses of silver nanoparticles (SNPs) using the unicellular green microalga Scenedesmus sp. Biosynthesized SNPs were characterized by AAS, UV-Vis spectroscopy, TEM, XRD, FTIR, DLS, and TGA studies and finally checked for antibacterial activity. Intracellular nanoparticle biosynthesis was initiated by a high rate of $Ag^+$ ion accumulation in the microalgal biomass and subsequent formation of spherical crystalline SNPs (average size, 15-20 nm) due to the biochemical reduction of $Ag^+$ ions. The synthesized nanoparticles were intracellular, as confirmed by the UV-Vis spectra of the outside medium. Furthermore, extracellular synthesis using boiled extract showed the formation of well scattered, highly stable, spherical SNPs with an average size of 5-10 nm. The size and morphology of the nanoparticles were confirmed by TEM. The crystalline nature of the SNPs was evident from the diffraction peaks of XRD and bright circular ring pattern of SAED. FTIR and UV-Vis spectra showed that biomolecules, proteins and peptides, are mainly responsible for the formation and stabilization of SNPs. Furthermore, the synthesized nanoparticles exhibited high antimicrobial activity against pathogenic gram-negative and gram-positive bacteria. Use of such a microalgal system provides a simple, cost-effective alternative template for the biosynthesis of nanomaterials in a large-scale system that could be of great use in biomedical applications.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.11 no.1
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• pp.26-36
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• 2000

A circularly polarized $12\times12$ array with application in the satellite communications is designed at 10 GHz. The radiator is an aperture-coupled ring patch, which is suitable of large arrays. The element spacing of the array is chosen to be $0.7\lambda_0$to maintain the main beam in the broadside direction. The array is a sequential array constructed on a series-parallel feed network to obtain high gain and low axial ratio. Measurement results for the array, acquired by experiments in the compact range of POSTECH, showed a directivity of 27.88 dB, a high gain of 25.55 dB, an efficiency of 60%, an axial ratio of 1.74 dB, and a side-lobe level of -13 dB. The bandwidth of the array was 43% when the VSWR was 2, and the bandwidth of the axial ratio was 16%.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.39C no.1
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• pp.47-59
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• 2014

This paper deals with a vision-based trajectory tracking control system for a quadrotor-type UAV for entertainment purpose in indoor environment. In contrast to outdoor flights that emphasize the autonomy to complete special missions such as aerial photographs and reconnaissance, indoor flights for entertainment require trajectory following and hovering skills especially in precision and stability of performance. This paper proposes a trajectory tracking control system consisting of a motion generation module, a pose estimation module, and a trajectory tracking module. The motion generation module generates a sequence of motions that are specified by 3-D locations at each sampling time. In the pose estimation module, 3-D position and orientation information of a quadrotor is estimated by recognizing a circular ring pattern installed on the vehicle. The trajectory tracking module controls the 3-D position of a quadrotor in real time using the information from the motion generation module and pose estimation module. The proposed system is tested through several experiments in view of one-point, multi-points, and trajectory tracking control.

• Journal of the Institute of Electronics Engineers of Korea TC
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• v.45 no.12
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• pp.139-145
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• 2008

In this paper, a microstrip surface wave antenna(SWA) with a frequency selective surface structure(FSS) is designed and measured. A microstrip SWA has many advantages such as low profile, low weight, easy fabrication, and compatibility with monolithic microwave integrated circuits(MMIC). In addition, it has demonstrated monopole like beam patterns. The microstrip SWA consists of two parts : a center-fed modified microstrip patch to excite surface wave, and a periodic patches to support the propagation of the surface waves. To obtain wide bandwidth, the ring type parasitic element is inserted and the circular patch is selected for the unit element in FSS structure. Experimental results show that the microstrip SWA has monopole like beam patterns at 5.9GHz. Impedance bandwidth and gain is 12% and 5.6dBi.