• 한국자원식물학회지
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• 제13권1호
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• pp.61-65
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• 2000

전라북도지방 소산 한약자원 식물은 91과 305속 470종 1아종 30변종 2품종으로 총 503종류가 본 조사결과 밝혀졌다. 각 과별로 그 숫자가 많은 종류는 국화과에 속하는 식물이 45종류로 가장 빈번하게 나타났고 그 다음은 장미과 식물이 30종류 그리고 콩과는 23종류의 순서였다. 전 조사지역에 고루 다수 분포하는 종류는 오미자, 마, 승마, 촛대승마, 족도리, 천남성, 둥굴레, 꿀풀, 박새를 들 수 있으며 집중적으로 나는 상태를 알아보기 위하여 정량조사를 실시한 결과, 예컨대, 더덕은 5m$\times$5m내에 30-50 개체가 덕유산 표고 약1300m 북서사면에서 조사되었다. 현재 심한 채취로 감소추세에 있는 식물은 둥굴레, 오미자, 더덕 등이고, 농가에서 재배중이거나 앞으로 개발 유망하며 가치가 높은 종류는 만삼, 가시오갈피, 천마, 복분자딸기, 더덕, 하수오 등이 있다. 요컨대 전라북도 지방에는 풍부하고 다양한 한약자원식물이 자연상태에 분포하고 있으며 이를 연구하고 집중적인 개발이 필요하다고 판단되었다.

• 한국환경복원기술학회지
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• 제16권1호
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• pp.109-130
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• 2013

This study is to offer the raw data for conservation and restoration of national park by surveying and analysing the vascular plants in the Gayasan national park. The flora were summarized as 102 families, 328 genera, 469 species, 4 subspecies, 59 varieties and 9 forms. The endangered plants designated by Ministry of Environment were 2 taxa; Pedicularis hallaisanensis Hurus. and Gymnadenia conopsea for. albiflora Y.N.Lee. The rare plants designated by Korea Forest Service were 13 taxa; Crypsinus hastatus (Thunb.) Copel., Ligusticum tachiroei (Franch. & Sav.) M. Hiroe & Constance, Primula modesta var. fauriae (Franch.) Takeda, Lilium distichum Nakai ex Kamibay and so forth. The korean endemic plants were 15 taxa; Betula ermanii Cham., Carpinus laxiflora (Siebold & Zucc.) Blume, Stewartia pseudocamellia Maxim., Galium koreanum (Nakai) Nakai, Heloniopsis koreana Fuse, N.S. Lee & M.N. Tamura and so forth. The specific plants by floristic region were 66 taxa; Betula chinensis Maxim., Spiraea fritschiana Schneid., Vaccinium hirtum var. koreanum (Nakai) Kitam., Cimicifuga heracleifolia Kom., Adoxa moschatellina L. and so forth. The plants with approval for delivering oversea were 22 taxa; Salix hallaisanensis H.L$\acute{e}$v., Sanguisorba argutidens Nakai, Viola albida Palib., Weigela subsessilis (Nakai) L.H.Bailey and so forth. The naturalized plants were 30 taxa; Chenopodium album L., Lepidium apetalum Willd., Trifolium pratense L., Bidens frondosa L., Helianthus tuberosus L. and so forth.

• Journal of Periodontal and Implant Science
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• 제45권4호
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• pp.145-151
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• 2015

Purpose: The aim of this study was to investigate the therapeutic effects of a herbal formula, PerioH-035, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum on the periodontal breakdown in a well-established ligature-induced periodontitis model in rats. Methods: Sprague-Dawley rats were randomly assigned to 1 of 4 groups: NL (non-ligatured), L (ligatured), P1 (ligatured and treated with 1 mg/mL PerioH-035), P100 (ligatured and treated with 100 mg/mL PerioH-035). Periodontitis was induced by placing a ligature around the mandibular first molars. PerioH-035 was topically applied to both sides of the first molar for 2 weeks. The right side of the mandibles was retrieved for micro-computed tomography (CT) and methylene blue staining to analyze alveolar bone loss. The left side of the mandibles was histologically analyzed by TRAP and H&E staining. The MMP-9 mRNA level in gingival tissue was investigated by RT-PCR. Results: Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups. The number of dense multi-nucleated cells found to be TRAP-positive by staining in the ligatured rats was markedly decreased by PerioH-035 application. In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups. Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation. Conclusions: These results suggest that PerioH-035 has therapeutic effects on periodontitis, and thus, PerioH-035 shows promise as a treatment for periodontitis.

• 대한수의학회지
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• 제40권2호
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• pp.221-228
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• 2000

We performed this study to determine the effect of Bu-Zhong-Yi-Qi-Tang, as a prescription of traditional Oriental medicine, and its major ingredients on jejunal crypt survival, endogenous spleen colony formation, apopotosis in jejunal crypt cells, lethality and hematological change of mice irradiated with high and low dose of Y-radiation. Bu-Zhong-Yi-Qi-Tang administration before irradiation protected the jejunal crypts (p<0.0001), increased the formation of endogenous spleen colony (p<0.05) and reduced the frequency of radiation-induced apoptosis (p<0.05). The survival rate and mean survival time of the groups treated with Bu-Zhong-Yi-Qi-Tang within 30 days after the treatment were far better than the irradiation control group. In the experiment on the effect of ingredients of Bu-Zhong-Yi-Qi-Tang, the result indicated that the extract of Rensan (Panax ginseng), Danggui (Angelica sinensis), Shengma (Cimicifuga heracleifolia) and Chaihu (Bupleurum falcatnosa) might have a major radioprotective effect. Although the mechanisms of this inhibitory effect remain to be elucidated, these results indicated that BU-Zhong-Yi-Qi-Tang might be a useful radioprotector, especially since it is a relatively nontoxic natural product. Further studies are needed to characterize better the protective nature of Bu-Zhong-Yi-Qi-Tang extract and its ingredients.

• 한국생물물리학회:학술대회논문집
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• 한국생물물리학회 2003년도 정기총회 및 학술발표회
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• pp.51-51
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• 2003

The medicinal plant Cimicifuga Racemosa (Black cohosh) has been used to treat many kinds of neuronal and menopausal symptoms, such as arthritis, menopausal depression, nerve pain, etc. Here, we examined the effect of Cimicifugoside (CF), one of triterpene glycosides which have been known as pharmacologically active ingredients of C. Racemosa, on nicotinic acetylcholine receptor (nAChR)-mediated catecholamine (CA) secretion in bovine adrenal chromaffin cell. Cimicifugoside inhibited calcium increase induced by 1,1-dimethyl-4-phenylpiperazinium iodide (DMPP), a nAChR agonist with a half maximal inhibitory concentration (IC50) of 18${\pm}$2${\mu}$M. In contrast, cimicifugoside did not affect the calcium increases evoked by high K$\^$+/, veratridine, and bradykinin. The DMPP-induced sodium increase was also inhibited by cimicifugoside with IC50 of 2${\pm}$0.3${\mu}$M, suggesting that the activity of nAChRs is inhibited by cimicifugoside. Cimicifugoside did not effect on the KCl-induced secretion but markedly inhibited the DMPP-induced catecholamine secretion which was monitored by carbon-fiber amperometry in real time, and by high performance liquid chromatography (HPLC) through electrochemical detection. The results suggest that cimicifugoside selectively inhibits nAChR-mediated response in bovine chromaffin cells.

• Journal of Radiation Protection and Research
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• 제26권1호
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• pp.27-33
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• 2001

Apoptosis의 유발을 억제하는 한약제를 파악하여 apoptosis와 관련된 정상적 또는 질병에 대한 연구에 자료를 제공하고자 방사선에 의해 유도된 apoptosis를 지표로 한의학적 처방에서 많이 사용되는 대표적 한약제(24종)의 효과를 검증하였다. 용안육, 산조인, 원지, 인삼, 복령 목향, 천궁 백작약, 승마, 시호 및 눈꽃동충하초 투여군에서 apoptosis는 감소되었으며, 이들 생약제는 apoptosis와 관련된 질병의 예방 및 치료에 적용할 수 있을 것이다. 추후 이들 생약의 작용 기전에 대한 연구가 요구된다.

• 한국환경생태학회지
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• 제21권5호
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• pp.415-431
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• 2007

운문산 자연휴식년제를 실시한 지역에 자생하는 관속식물은 83과 235속 338종 3아종 54변종 12품종 총 407분류군으로 동정되었다. 멸종위기야생식물은 산작약과 솔나리 2분류군이 관찰되었으며, 한국특산식물은 개비자나무, 참개별꽃, 진범, 할미밀망, 털둥근이질풀, 자주꿩의다리 등을 포함한 13과 19속 15종 4변종 2품종 총 21분류군이 확인되었다. 식물구계학적 특정식물은 고란초, 승마, 산작약, 솔나리, 천마 등 35과 50속 51종 4변종 총 55분류군이 관찰되었다. 귀화식물은 4과 10속 9종 1변종 총 10분류군이며 도시화지수(U.I) 및 귀화율(N.I)은 3.51%, 2.46%이다. 생활형조성은 $H-R_5-D_4-e$ type으로 나타났다.

• 한국환경복원기술학회지
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• 제15권1호
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• pp.89-105
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• 2012

This study is carried out to survey and analyse the vascular plants distributed around the Jusan Reservoir, Juwangsan national park. The results are as follows. The numbers of vascular plants were 377 taxa; 85 families, 253 genera, 332 species, 3 subspecies, 37 varieties and 5 forma. The endangered plants designated by Ministry of Environment were 2 taxa; Berchemia berchemiaefolia (Makino) Koidz. and Iris odaesanensis Y.N.Lee. The rare plants designated by Korea Forest Service were 6 taxa; Eranthis stellata Maxim., Aristolochia contorta Bunge, Aristolochia manshuriensis Kom., Berchemia berchemiaefolia (Makino) Koidz., Scopolia japonica Maxim. and Iris odaesanensis Y.N.Lee. The endemic plants were 4 taxa; Carpinus laxiflora (Siebold & Zucc.) Blume, Pseudostellaria coreana (Nakai) Ohwi, Philadelphus schrenkii Rupr. and Weigela subsessilis (Nakai) L.H.Bailey. The specific plants by floristic region were 38 taxa; Juglans mandshurica Maxim., Salix chaenomeloides Kimura, Hylomecon vernalis Maxim., Lonicera praeflorens Batalin, Erythronium japonicum (Baker) Decne., Potentilla dickinsii Franch. & Sav., Celtis aurantiaca Nakai, Anemone reflexa Steph. & Willd., Cimicifuga heracleifolia Kom. and so forth. The naturalized plants were 20 taxa; Fallopia dumetorum (L.) Holub, Chenopoidum album L., Lepidium apetalum Willd., Veronica persica Poir., Conyza canadensis (L.) Cronquist, Taraxacum officinale Weber and so forth.

• 사상체질의학회지
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• 제25권3호
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• pp.208-217
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• 2013

Objectives Modified Yeolda-Hanso tang (MYH) is a traditional herbal formula in Korea for various diseases. MYH is containing the 10 herbs : Pueraria lobata (Willd.) Ohwi, Angelica tenuissima Nakai, Scutellaria baicalensis Georgi, Platycodon grandiflorum (Jacq), Angelicae Dahurica, Cimicifuga heracleifolia Kom, Raphanus sativa L., Polygala tenuifolia (Willd), Acorus gramineus Soland and Dimocarpus longan Lour. The 10 herbs is constituted as a ratio of the 6:4:2:1:2:2:2:4:6:6. We investigated neuroprotective effects of MYH on human neuroblastoma SH-SY5Y cells and evaluated the ability of MYH to prevent and treat for neurodegenerative diseases such as Parkinson's disease via basal autophagy enhancement. Methods Pharmacological induction of Autophagy by MYH in SH-SY5Y cells: Induction of autophagy by MYH in human neuroblastoma SH-SY5Y cells was carreid out by immunoblot analysis with several autophagy markers. SH-SY5Y cells were treated with MYH at the concentration of 400 and $800{\mu}g/ml$ for 24 hr. Specifically, the autophagosome proteins LC3 II and Atg5 levels were increased and autophagy pathway related proteins such as beclin-1, PI3 Kinase class III protein, ULK1, mTOR and AMPK were activated. Conclusions MYH can enhance the induction of autophagy through key regulator AMPK, mTOR, and Beclin-1 and it should be considered as a possible candidate of neuroprotective agents for such as Parkinson's disease.

• 한방안이비인후피부과학회지
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• 제15권2호
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• pp.80-103
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• 2002

To screen the effective materials for hair loss treatment, several natural extracts were tested using in vivo and in vitro test models. Firstly, all test materials were applicated onto the back skin of C57BL／6 mouse and then hair growth pormoting effect were measured using hair growth index As a result, Polygonum muitifiorum Thunb and Terrninalia chebula Retz. showed potent hair growth promoting effect, ranking as 1.5-2.0 of hair growth index. However, there were no plant extracts, which have remarkable potential of growth promotion of human hair dermal papilla cells cultured in vitro. In the experiments of 5${\alpha}$-reductase type Ⅱ inhibition assay, Morus alba L., Chaenomelis Fructus, Saussureae Radix, Angelicae Gigantis Radix, Polygonum multifiorum Thunb, and Angelica dahurica (Fischer) Bentham et Hooker f. showed effective potential to inhibit the activity of 5${\alpha}$-reductase type Ⅱ. To investigate the possible involvement of effects of several plant extracts on the gene expression of growth factors in human hair dermal papilla cells, RT-PCR analyses were performed. As a consequences, Mentha haplocalyx Briq., Cimicifuga foetida L., Eclipta prostrata (L.) L., Pinus densiflora S. et. Z, and Polygonum muitifiorum Thunb revealed the regulatory roles on the expression of growth factors such as IGF-I, KGF, HGF and VEGF in the dermal papilla cells. Another test for inhibition of microbial such as P. acne and P. ovale were also carried out to find whether these plant extracts have anti-microbial activities. Morus alba L. and Chaenomelis Fructus showed anti-microbial effects on Propionibacterium acnes, which is believed as a pathogen of acne. Together, these results showed several plant extracts can be used for hair growth promotion.