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http://dx.doi.org/10.5051/jpis.2015.45.4.145

Topical application of herbal formula for the treatment of ligature-induced periodontitis  

Kim, Mi Hye (Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine)
Choi, You Yeon (Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine)
Lee, Hye Ji (Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine)
Lee, Haesu (Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine)
Park, Jung-Chul (Department of Periodontology, Dankook University College of Dentistry)
Yang, Woong Mo (Department of Convergence Korean Medical Science, Kyung Hee University College of Korean Medicine)
Publication Information
Journal of Periodontal and Implant Science / v.45, no.4, 2015 , pp. 145-151 More about this Journal
Abstract
Purpose: The aim of this study was to investigate the therapeutic effects of a herbal formula, PerioH-035, containing Angelica sinensis, steamed Rehmannia glutinosa, Angelica dahurica, Cimicifuga heracleifolia, and Zanthoxylum piperitum on the periodontal breakdown in a well-established ligature-induced periodontitis model in rats. Methods: Sprague-Dawley rats were randomly assigned to 1 of 4 groups: NL (non-ligatured), L (ligatured), P1 (ligatured and treated with 1 mg/mL PerioH-035), P100 (ligatured and treated with 100 mg/mL PerioH-035). Periodontitis was induced by placing a ligature around the mandibular first molars. PerioH-035 was topically applied to both sides of the first molar for 2 weeks. The right side of the mandibles was retrieved for micro-computed tomography (CT) and methylene blue staining to analyze alveolar bone loss. The left side of the mandibles was histologically analyzed by TRAP and H&E staining. The MMP-9 mRNA level in gingival tissue was investigated by RT-PCR. Results: Alveolar bone resorption was significantly reduced in the PerioH-035-treated groups. The number of dense multi-nucleated cells found to be TRAP-positive by staining in the ligatured rats was markedly decreased by PerioH-035 application. In addition, periodontal tissue destruction, especially cementum demineralization, was ameliorated in the P1 and P100 groups. Moreover, gingival tissue from the PerioH-035-treated group showed a decrease in the MMP-9 mRNA level, resulting in recovery of collagen degradation. Conclusions: These results suggest that PerioH-035 has therapeutic effects on periodontitis, and thus, PerioH-035 shows promise as a treatment for periodontitis.
Keywords
Matrix metalloproteinase 9; Medicinal herbs; Osteoclasts; Periodontitis;
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Times Cited By KSCI : 2  (Citation Analysis)
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