• 대한수의학회지
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• 제19권2호
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• pp.143-147
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• 1979

Eight Holstein cows weighing 419-673kg with 2 to 10 years of age were allocated into two groups, four animals each, to study the effect of feeding urea and urea plus Zeolite on rumen pH and the population of rumen ciliates. Rumen fluid samples were taken after morning feed for 7 times at one hour intervals. Experimental results obtained were as follows; 1. Rumen pH was gradually dropped from 7.272 to 7.163 by 3.01 hour and from 7.18 to 7.07 by 2.87 hour and reached 7.352 and 7.29 at 7 hour after feeding, respectively on urea and urea plus Zeolite feeding group. 2. Total ciliate numbers decreased from 209,540 to 113,755 by 4.311 hour and from 381,430 to 203.125 by 4.406 hour and gradually increased to 151,030 and 265,230 by 7 hour after feeding, respectively on urea and urea plus Zeolite feeding group. 3. Population of ruminal ciliates was not changed and Entodinium simplex was the major ciliate population for both treatments. It was from 81.21 to 89.12% on urea feeding group and from 84.6 to 88.3% of total number of ciliates on urea plus Zeolite feeding group.

• 한국미생물·생명공학회지
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• 제32권2호
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• pp.158-165
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• 2004

Pilot Plant 에서 도시하수가 처리되는 경우와 전 처리된 분뇨가 하수에 섞여 처리되는 두 가지 종류의 처리장하수를 대상으로 식종과 무식종의 경우로 나누어 활성슬러지의 정착 단계까지 조사하였다. 원생동물의 천이과정은 편모충류, 육질충류, 섬모충류 순이며, 섬모충류 내에서는 자유 유영형이 먼저 출현하고 포복형과 고착형이 차츰 증가하여 활성슬러지 원생동물상을 구성한다. 정상적으로 가동되는 처리장 포기조의 원생동물분포는 시운전초기단계의 원생동물 분포와 다르며, 시운전 안정화 단계에서의 원생동물 분포와 유사하였다. 포기조의 원생동물은 유입수에서 유래하는 것으로 판단되며, 포기조에 유입된 후 환경조건에 맞는 종이 증식하여 활성슬러지를 구성한다. 슬러지식종은 활성슬러지 원생동물 군집구조 형성에 영향을 미치지 못했으나 활성슬러지의 안정화기간을 다소 단축시키는 효과가 있었다. 처리장별로 활성슬러지 원생동물의 종류는 비슷하나 개체수는 달라 유입수의 성상에 영향을 받는 것으로 판단된다. Pilot plant 가동 후반기에는 섬모충류 비율이 70%전후, 개체수도 3,000∼5,000 개체/$m\ell$이며, 유출수의 BOD 농도도 안정적으로 나타나 정상적으로 가동된다고 판단된다.

• 환경생물
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• 제33권4호
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• pp.375-382
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• 2015

We discovered three soil ciliates of the class Colpodea-Colpoda henneguyi Fabre-Domergue, 1889; C. lucida Greeff, 1888; and Bursaria truncatella $M{\ddot{u}}ller$, 1773 from Obong-ri, Ayajin-ri and Elwang-ri (Korea), respectively. Colpoda henneguyi had the following features: often wider preorally than postorally, size in vivo $60-80{\mu}m{\times}50-70{\mu}m$; extrusomes indistinct in vivo, cylindroid approximately $1{\mu}m$ long; notches caused by deep diagonal groove; yellowish globules on the cortex of the cell; 10-12 postoral kineties; silverline system aspera-type. Colpoda lucida exhibited the following features: broadly reniform, size in vivo $70-90{\mu}m{\times}50-70{\mu}m$; conspicuous extrusomes, $3.5-5{\mu}m$ long in vivo, cylindroid to fusiform; 13-16 postoral kineties; silverline system cucullus-type. Bursaria truncatella had the following features: bursiform, size in vivo $300-470{\mu}m{\times}120-260{\mu}m$; macronucleus coiled with highly variable shapes, $600-1100{\mu}m{\times}30-40{\mu}m$ long in vivo; micronuclei 16-25 in number, approximately $4{\mu}m$ in diameter; extrusomes cylindroid, $3-4{\mu}m$ long in vivo. This is the first report of colpodean ciliates from Korea, and we describe these species based on observations of live and impregnated (protargol and silver nitrate impregnation) specimens.

• Animal cells and systems
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• 제13권3호
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• pp.351-356
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• 2009

Ciliates are undoubtedly one of the most diverse protozoans that play a significant role in ecology. However, molecular examination, based on comparing the DNA sequences, has been done on a limited number of the species. Because most ciliates are uncultivable and their population sizes are often too small, it is usually difficult to obtain sufficient genomic DNA required for PCR based experiments. In the present study, we evaluated the effectiveness of four commercial DNA extraction procedures that extract high quality genomic DNA from a single ciliate cell. It was discovered that RED Extract-N-$Amp^{TM}$ PCR kit is the best method for removing PCR-inhibiting substances and minimizing DNA loss during purification. This method can also amplify more than 25 reactions of PCR. In addition, this technique was applied to single cells of 19 species belonged to 7 orders under 5 classes that isolated from mixed natural populations. Their small subunit ribosomal DNA (SSU rDNA) was successfully amplified. In summary, we developed a simple technique for the high-yield extraction of purified DNA from a single ciliate cell that may be more useful for rare ciliates, such as tiny and uncultivable marine microbes.

• 한국미생물·생명공학회지
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• 제28권6호
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• pp.361-366
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• 2000

Genera and number of protozoa were investigated in the conventional activated sludge pilot plant used for the treatment of municipal sewage and pre-treated night soil-containing sewage. In both case, the predominant protozoa was ciliates and among them Vorticella was the most common. In the pilot plant where pre-treated night soil was mixed with municipal sewage, genera of free-swimming ciliates, flagellates and amoeba was higher than in those withour night soil. Correlation analysis on the quality of effluent and protozoa indicates that municipal sewage has positive correlation with protozoa. However in the pilot plan 샐 sewage contatinin pre-treated night coil soil more samples show negative correlation. Followed equations were derived by the regression analysis of BOD in both the pilot plants. In case of pilot plant A of municipal sewage, the analysis B of munici-pal and pre-treated night soil-containing sewage, the analysis of BOD was $6.731$\times$10_{-2}$ $\times$Bodo+0.306(Adjusted $R^2$=0.864). At low temperature, number of protozoa was decreased to 35% and among therm, Aspidisca was the most common genus. Therefore, protozoa can be used as indicator of quality of the effluent in sewage treatment plants.

• Animal Systematics, Evolution and Diversity
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• 제26권3호
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• pp.307-316
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• 2010

To investigate the Korean ciliate fauna, two oxytrichid ciliates, Laurentiella strenua (Dingfelder, 1962) and Notohymena australis (Foissner and O'Donoghue, 1990) were collected from the Taehwa River in Ulsan, South Korea. These two ciliates are reported for the first time in Korea. Descriptions were based on the observation of live and silver stained specimens. Diagnoses of these species are as follows: Laurentiella strenua: body is approximately $320{\times}170\;{\mu}m$ in size, an inverted triangular shape. Cytoplasmic globules are present in both anterior and posterior margins, transparent and sized $10-15\;{\mu}m$ in live specimens. Adoral zone of membranelles is covering about 50% of body length. Frontoventral-transverse cirri are arranged diagonally into 5 rows. Transverse cirri are arranged almost longitudinally and varied 4-6 in number. Dorsal kineties are 11 rows with multiple fragmentations. Macronuclei are 4-5 in number. Notohymena australis: body is approximately $170{\times}50\;{\mu}m$ in live specimens, slender and long ellipsoidal shape. Yellowish green cortical granules are present as pairs or rows which composed of 3-6 granules. Adoral zone of membranelles is covering about 36% of the body length, composed of 39-50 adoral membranelles. Dorsal kineties is 6 rows. Macronuclei are 2 in number.

• Animal Systematics, Evolution and Diversity
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• 제25권2호
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• pp.167-178
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• 2009

Two poorly known and often confused pleurostomatid ciliates, Litonotus paracygnus Song, 1994 and L. pictus Gruber, 1884, were collected from the coastal waters of Yeonggeumjeong and Bongpo-port, Gangwondo in the East Sea and from the Iwon tide embankment near Ganwol-do, Chungcheongnam-do in the Yellow Sea, Korea. These species were described based on live observations, the protargol-impregnation and morphometrics of the species. Also provided are their diagnoses. The small subunit ribosomal DNA (SSU rDNA) sequences of these species were compared with previously known sequences of related species. The diagnostics of the two Litonotus species are as follows. L. paracygnus: 150-300 $\mu$m long in vivo, strongly contractile neck region, two ellipsoid macronuclei (Ma) and one micronucleus (Mi), 7 left (LSK) and 11-14 right somatic kineties (RSK), 2-4 contractile vacuoles (CV) located on the posterior end, extrusemes (Ex) distributed on the anterior region of the ventral margin only. L. pictus: about 200-600 $\mu$m long in vivo, extremely contractile, beautiful body color with rows of yellow to yellow-brownish cortical pigment granules, 12-21 Ma arranged in moniliform pattern, infrequently vermiform, 7-11 LSK and 18-26 RSK, several CV located on both margins, Ex distributed on the anterior region of the ventral margin only. In this study, this genus was firstly recorded in Korea.

• Journal of Species Research
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• 제9권3호
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• pp.251-268
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• 2020

Ciliates are a speciose protozoan group and their diversity is still not well investigated in Korea. From our field surveys, 18 unrecorded ciliate species were collected from a total of nine localities in Korea. The field surveys were conducted in 2018 and 2019, and the unrecorded ciliates were found from terrestrial (moss and soil samples) and inland water (river and lagoon samples) habitats. The morphology of these ciliates was studied using the observation of living and silver impregnated (protargol) specimens. The present species belong to five classes as follows: 1) class Spirotrichea - Gonostomum kuehnelti, Quadristicha setigera, Lamtostyla decorata, L. islandica, L. longa, Anteholosticha brachysticha, Pseudobirojimia muscorum; 2) class Litostomatea - Apocoriplites lajacola, Epispathidium amphoriforme, Protospathidium muscicola, Phialinides australis, Paraenchelys wenzeli, Rimaleptus similis; 3) class Nassophorea - Drepanomonas revoluta; 4) class Colpodea - Bryometopus triquetrus; and 5) class Oligohymenophorea - Cyclidium glaucoma, Epistylis pygmaeum, Pseudovorticella vestita. The globular macronuclear nodules of P. vestita were observed for the first time in the Korean population.

• 한국수산과학회지
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• 제35권4호
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• pp.342-347
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• 2002

섬모충은 윤충류보다 다양한 크기를 가지고 있으며, 얇은 세포벽, 느린 운동성, 빠른 번식률로 새로운 동물성 먹이생물로써 개발가능성을 가지고 있다. 한국 연안 수역인 수영만, 광양만 및 경기만 소래 염전지역에서 식물성 플랑크톤 네트를 이용하여 6종의 섬모충을 채집하였으며, capillary pipette로 분리 $\cdot$배양하였다. 먹이생물로써의 개발가능성을 파악하기 위하여 크기, 운동성, 배양밀도 및 부유능력을 평가하였으며, 채집된 섬모충류 가운데 광양만에서 채집된 Euplotes sp. (K-1)가 가장 적합한 것으로 평가되었다. MErds 기본배지에 glycine, glucose, yeast extract을 각 0.1 g씩 첨가하여 변형한 MErds-2 배지는 기본배지보다도 약 6배의 번식률을 보여, Euplotes sp.를 비롯한 기타 섬모충 배양배지로도 유용하였다. Euplotes sp. (K-1)은 수온 $22.5^{\circ}C$, pH 8,조도 2,000 lux에서 각각 최고의 번식률을 보였으며, 이들 환경조건에 대해 비교적 폭넓은 환경적응력을 나타내었다. 한편, 빵효모 1.0 g/L의 첨가에 의해서도 대량배양이 가능하였다. 본 연구에서의 섬모충은 종보관이 용이하며 폭넓은 환경 적응력과 간단한 방법의 대량배양이 가능하여 초기 동물성 먹이생물로써의 높은 잠재력을 가지고 있는 것으로 평가되었다.

• 한국해양학회지:바다
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• 제8권2호
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• pp.59-69
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• 2003

제주도 남부해역의 미세생물 먹이망 구조에 관한 연구의 일환으로써 부유성 유종섬모충(tintinnids)과 빈섬모충(aloricate oligotrichs)의 종 조성과 생물량의 계절 변화 특성에 대하여 중문주변해역의 6개 정점에서 1998년 7월부터 2000년 6월까지 매월 조사를 실시하였다. 조사기간 동안 출현한 유종섬모충은 총 39종으로 세포수 범위는 100~5,400 cells. 1$^{-1}$(평균 314 cells . 1$^{-1}$) 이었고 가을철과 겨울철에는 외양성 종류가 주로 우점하였으며 봄철과 여름철은 외양성 보다 연안성 종류가 우세하였다. 빈섬모충은 총 15종이 동정되었고 세포수 범위는 140~21,000 cells.1$^{-1}$(평균 2,356 cells.1$^{-1}$)로써 Strombidium속의 종들이 전 계절에 걸쳐 우점하였다. 계절에 따른 종 다양성과 생물량 변화는 유종섬모충의 경우 차이가 컸지만 빈섬모충은 큰 차이가 없었다. 유종섬모충의 계절별 수괴지표 특성은 연구 해역의 해양환경변화에 따라 영향을 받고 있었으나 빈섬모충은 특이한 수괴지표 특성을 보이지 않았다. 해역별로 보면 외해역 보다는 해안역에서 세포수가 높았고 종 다양성도 높았다 섬모충의 총 탄소량의 범위는 0.01~136.06 $\mu\textrm{g}$C.1$^{-1}$(평균 5.01 $\mu\textrm{g}$C.1$^{-1}$)로 탄소량과 세포수의 월별 변동이 반드시 일치하지는 않았다. 유종섬모충과 빈섬모충의 수심별세포수 분포는 모두 20 m층에서 가장 높았고 수직분포의 경향도 비슷하였으며, 엽록소 a 량의 수직분포와도 일치하고 있어 식물플랑크톤과 섬모충은 피식과 포식의 상관성을 보이고 있었다.