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http://dx.doi.org/10.5635/KJSZ.2009.25.2.167

Taxonomic Study of Poorly-known Marine Pleurostomatid Ciliates of Litonotus paracygnus and L. pictus (Ciliophora: Pleurostomatida) from Korea  

Kim, Se-Joo (Institute for Basic Science, Inha University)
Min, Gi-Sik (Department of Biological Sciences, Inha University)
Publication Information
Animal Systematics, Evolution and Diversity / v.25, no.2, 2009 , pp. 167-178 More about this Journal
Abstract
Two poorly known and often confused pleurostomatid ciliates, Litonotus paracygnus Song, 1994 and L. pictus Gruber, 1884, were collected from the coastal waters of Yeonggeumjeong and Bongpo-port, Gangwondo in the East Sea and from the Iwon tide embankment near Ganwol-do, Chungcheongnam-do in the Yellow Sea, Korea. These species were described based on live observations, the protargol-impregnation and morphometrics of the species. Also provided are their diagnoses. The small subunit ribosomal DNA (SSU rDNA) sequences of these species were compared with previously known sequences of related species. The diagnostics of the two Litonotus species are as follows. L. paracygnus: 150-300 $\mu$m long in vivo, strongly contractile neck region, two ellipsoid macronuclei (Ma) and one micronucleus (Mi), 7 left (LSK) and 11-14 right somatic kineties (RSK), 2-4 contractile vacuoles (CV) located on the posterior end, extrusemes (Ex) distributed on the anterior region of the ventral margin only. L. pictus: about 200-600 $\mu$m long in vivo, extremely contractile, beautiful body color with rows of yellow to yellow-brownish cortical pigment granules, 12-21 Ma arranged in moniliform pattern, infrequently vermiform, 7-11 LSK and 18-26 RSK, several CV located on both margins, Ex distributed on the anterior region of the ventral margin only. In this study, this genus was firstly recorded in Korea.
Keywords
pleurostomatid ciliates; Litonotus paracygnus; Litonotus pictus; SSU rDNA; Korea;
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1 Barth, D., S. Krenek, S.I. Fokin and T.U. Berendonk, 2006. Intraspecific genetic variation in Paramecium revealed by mitochondrial cytochrome C oxidase I sequences. J. Eukaryot. Microbiol., 53: 20-25   DOI   ScienceOn
2 Chantangsi, C., D.H. Lynn, M.T. Brandl, J.C. Cole, N. Hetrick and P. Ikonomi, 2007. Barcoding ciliates: a comprehensive study of 75 isolates of the genus Tetrahymena. Int. J. Syst. Evol. Microbiol., 10: 2412-2425
3 Dragesco, J., 1966. Observations sur quelques cili$\s libres. Arch. Protistenk., 109: 155-206
4 Foissner, W., 1984. Taxonomie und O¨ kologie einiger Ciliaten (Protozoa, Ciliophora) des Saprobiensystems. I: Genera Litonotus, Amphileptus, Opisthodon. Hydrobiologia, 119: 193-208   DOI
5 Foissner, W. and D. Leipe, 1995. Morphology and ecology of Siroloxophyllum utriculariae (Penard, 1922) n. g., n. comb. (Ciliophora, Pleurostomatida) and an improved classification of pleurostomatid ciliates. J. Eukaryot. Microbiol., 42: 476-490   DOI   ScienceOn
6 Gruber, A., 1884. Die Protozoen des Hafens von Genua. Nova. Acta Leop. Carol. 46: 521-523
7 Kahl, A., 1933. Ciliata Libera et Ectocommensalia. In Grimpe, G. and E. Wagler, eds., Die Tierwelt der Nord- und Ostsee. Lief 23 (Teil II, c3). Leipzig, pp. 29-146
8 Lee, J.M., J. Yoon and M.K. Shin, 2006. Two litonotid ciliates (Ciliophora: Litostomatea: Pleurostomatida) unknown from Korea. Korean J. Syst. Zool., 22: 217-221
9 Li, L., 1990. A new species of ciliates, Hemiophrys polymicronuclei sp. nov. from Donghu Lake, Hubei Province. Chin. J. Oceanol. Limnol., 8: 97-100   DOI
10 Petz, W., W. Song and N. Wilbert, 1995. Taxonomy and ecology of the ciliate fauna (Protozoa, Ciliophora) in the endopagial and pelagial of the Weddell Sea, Antarctica. Stapfia, 40: 1-23
11 Song, W.B. and N. Wilbert, 1989. Taxonomische Untersuchungen an Aufwuchsciliaten (Protozoa, Ciliophora) im Poppelsdorfer Weiher, Bonn. Lauterbornia, 3: 2-221
12 Tautz, D., P. Arctander, A. Minelli, R.H. Thomas and A.P. Vogler, 2003. A plea for DNA taxonomy. Trends Ecol. Evol., 18: 70-74   DOI   ScienceOn
13 Dragesco, J. and A. Dragesco-Kerneis, 1986. Ciliés libres de 1'Afrique intertropicale. Faune Trop., 26: 1-559
14 DeSalle, R., M.G. Egan and M. Siddall, 2005. The unholy trinity: taxonomy, species delimitation and DNA barcoding. Philos. Trans. R. Soc. Lond. B. Biol. Sci., 360: 1905-1916   DOI   ScienceOn
15 Lin, X. and W. Song, 2004. Establishment of a New Amphileptid Genus, Apoamphileptus nov. gen. (Ciliophora, Litostomatea, Pleurostomatida), with Description of a New Marine Species, Apoamphileptus robertsi nov. spec. from Qingdao, China. J. Eukaryot. Microbiol., 51: 618-625   DOI   ScienceOn
16 Lin, X.F., J.Q. Li, J. Gong, A. Warren and W. Song, 2008. Taxonomic studies on three marine pleurostomatid ciliates, Litonotus bergeri nov. spec., L. blattereri nov. spec. and L. petzi nov. spec. (Ciliophora, Pleurostomatida) from North China Sea. Eur. J. Protist., 44: 91-102   DOI   ScienceOn
17 Prescott, D., 1994. The DNA of ciliated protozoa. Microbiol. Rev., 58: 233-267   PUBMED
18 Tamura, K., J. Dudley, M. Nei and S. Kumar, 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol. Biol. Evol., 24: 1596-1599   DOI   ScienceOn
19 Kim, S.Y., S.J. Kim, G.S. Min, E.J. Yang, M.H. Yoo and J.K. Choi, 2007. Analysis of genetic variation in the small subunit ribosomal RNA gene of Euplotes ciliates for developing species diagnostic molecular marker. The Sea, 12: 225- 233
20 Song, W., 1994. Morphology and infraciliature of a new marine ciliate, Litonotus paracygnus nov. sp. (Ciliophora, Pleurostomatida). Acta Zool. Sin., 40: 131-136 (in Chinese with English summary)
21 Foissner, W., 1991. Basic light and scanning electron microscopic methods for taxonomic studies of ciliated protozoa. Europ. J. Protistol., 27: 313-330   DOI
22 Fryd-Versavel, G., F. Tftode and J. Dragesco, 1975. Contribution $\`{a}$ la connaissance de quelques ciliés gymnostomes. II. Prostomiens, pleurostomiens: morphologie, stomatogen$\`{e}$se. Protistologica, 11: 509-530
23 Gong, J., S.J. Kim, S.Y. Kim, G.S. Min, D.McL. Roberts, A. Warren and J.K. Choi, 2007b. Taxonomic Redescriptions of Two Ciliates, Protogastrostyla pulchra n. g., n. comb. and Hemigastrostyla enigmatica (Ciliophora: Spirotrichea, Stichotrichia), with Phylogenetic Analyses Based on 18S and 28S rRNA Gene Sequences. J. Eukaryot. Microbiol., 54: 468-478   PUBMED
24 Lin, X.F., W. Song and A. Warren, 2005. Two new marine pleurostomatid ciliates from China, Amphileptus gui nov. spec. and Amphileptus yuianus nov. spec. (Ciliophora, Pleurostomatida). Eur. J. Protist., 41: 163-173   DOI   ScienceOn
25 Foissner, W., H. Blatterer, H. Berger and F. Kohmann, 1995. Taxonomische und $\"{o}$kologische Revision der Ciliaten des Saprobiensystems. Band IV: Gymnostomatea, Loxodes, Suctoria. Bayerischen Landesamtes fur Wasserwirtschaft, Deggendorf, pp. 318-325
26 Lynn, D.H., 2008. The ciliated protozoa: characterization, classification, and guide to the literature. Springer, New York, pp. 1-605
27 Xu, H., G.S. Min, J.K. Choi, S.J. Kim, J.H. Jung and B.J. Lim, 2009. An investigation on periphytic ciliate colonization of an artificial substrate in Korean coastal waters. J. Mar. Biol. Assoc. UK., 89: 669-679   DOI   ScienceOn
28 Corliss, J.O., 1979. The Ciliated Protozoa. Pergaman Press, New York, pp. 1-455
29 Gong, J., J.K. Choi, D.M. Roberts, S.Y. Kim and G.S. Min, 2007a. Morphological descriptions of new and little-known benthic ciliates from ganghwa tidal flat, Korea. J. Eukaryot. Microbiol., 54: 306-316   DOI   ScienceOn
30 Lin, X.F., W. Song and A. Warren, 2005. Two new marine pleurostomatid ciliates from China, Amphileptus gui nov. spec. and Amphileptus yuianus nov. spec. (Ciliophora, Pleurostomatida). Eur. J. Protist., 41: 163-173   DOI   ScienceOn
31 Borror, A.C., 1963. Morphology andecology of the benthic ciliated protozoa of Alligator Habor, Florida. Arch. Protistenkd., 106: 465-534
32 Kahl, A., 1931. Urtiere oder Protozoa I: Wimpertiere oder Ciliata (Infusoria) 2. Holotricha. Tierwelt Dtl., 21: 181-398
33 Song, W., 1993. Studies on the morphology and systematic status of Loxophyllum rostratum Cohn, 1866 (Ciliophora, Pleurostomatida). J. Oceanogr. Huanghai Bohai Sea, 11: 44-49 (in Chinese with English summary)