• Journal of Microbiology and Biotechnology
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• 제28권4호
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• pp.645-651
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• 2018

The carcinogenicity of chemicals in the environment is a major concern. Recently, numerous studies have attempted to develop methods for predicting carcinogenicity, including rodent and cell-based approaches. However, rodent carcinogenicity tests for evaluating the carcinogenic potential of a chemical to humans are time-consuming and costly. This study focused on the development of an alternative method for predicting carcinogenicity using quantitative PCR (qPCR) and colon cancer stem cells. A toxicogenomic method, mRNA profiling, is useful for predicting carcinogenicity. Using microarray analysis, we optimized 16 predictive gene sets from five carcinogens (azoxymethane, 3,2'-dimethyl-4-aminobiphenyl, N-ethyl-n-nitrosourea, metronidazole, 4-(n-methyl-n-nitrosamino)-1-(3-pyridyl)-1-butanone) used to treat colon cancer stem cell samples. The 16 genes were evaluated by qPCR using 23 positive and negative carcinogens in colon cancer stem cells. Among them, six genes could differentiate between positive and negative carcinogens with a p-value of ${\leq}0.05$. Our qPCR-based prediction system for colon carcinogenesis using colon cancer stem cells is cost- and time-efficient. Thus, this qPCR-based prediction system is an alternative to in vivo carcinogenicity screening assays.

• Journal of Microbiology and Biotechnology
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• 제17권7호
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• pp.1152-1161
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• 2007

An immunochromatography (ICG) strip test based on a monoclonal antibody for the rapid detection of L. monocytogenes in meat and processed-meat samples was developed in this study. A monoclonal antibody (MAb) specific to L. monocytogenes was produced from cloned hybridoma cells (FKLM-3B12-37) and used to develop an ICG strip test. The antibody showed a stronger binding to L. monocytogenes than other Listeria species, and a weak cross-reaction to S. aureus based on an ELISA. The detection limit of the ICG strip test was $10^5\;cell/ml$. In total, 116 meat and processed-meat samples were collected and analyzed using both the ICG strip test and a PCR. The ICG strip test and PCR indicated L. monocytogenes contamination in 34 and 27 meat samples, respectively. The 7 meat samples not identified as L. monocytogenes positive by the PCR were also tested using an API kit and found to be contaminated by Listeria species. In conclusion, the ICG strip test results agreed well with those obtained using the PCR and API kit. Thus, the developed ICG has potential use as a primary screening tool for L. monocytogenes in various foods and agricultural products, generating results within 20 min without complicated steps.

• Journal of the Korean Academy of Child and Adolescent Psychiatry
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• 제21권3호
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• pp.153-160
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• 2010

Objectives: The purpose of this study was to evaluate the prenatal, perinatal, and infancy history of children with autism spectrum disorder (ASD) as compared to unaffected siblings (SIB) and typically developing children (TC). Methods: Subjects with ASD, their SIB, and TC were recruited. All subjects were assessed using both the Korean version of Autism Diagnostic Interview-Revised (K-ADI-R) and the Korean version of Autism Diagnostic Observation Schedule (K-ADOS) and were subsequently identified as affected or unaffected. Prenatal, perinatal, and infancy history was obtained from the primary caregivers and each facet was compared in those with ASD, the SIB, and the TC groups using SPSS ver. 17.0 (p<.05). Results: 70 individuals with ASD (63 males, 87.94${\pm}$37.8months), 53 SIB (27 males, 85.4087.94${\pm}$48.06 months), and 32 TC (19 males, 104.1987.94${\pm}$23.409 months) were analyzed. The ASD group showed significantly higher rates of insufficient vaccination as they aged age ($x^2$=15.54, p=.000). Among the scheduled vaccinations, the DPT vaccination ($x^2$=10.08, p=.006) was insufficient in ASD groups. The ASD group also showed higher rates of sleep disturbances from infancy. Differences in maternal/paternal age at conception, gestational age, and growth parameters at birth were not significantly difference among the three groups. Conclusion: These results do not support the previous controversies regarding the relationship between prenatal/perinatal complications and ASD. However, these results indicate that perinatal and prenatal factors may contribute to the development of ASD.

• Journal of the Institute of Electronics Engineers of Korea SC
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• 제47권6호
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• pp.12-18
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• 2010

Automatic Target Detection (ATD) systems that use forward-looking infrared (FLIR) consists of three stages. preprocessing, detection, and clutter rejection. All potential targets are extracted in preprocessing and detection stages. But, this results in a high false alarm rates. To reduce false alarm rates of ATD system, true targets are extracted in the clutter rejection stage. This paper focuses on clutter rejection stage. This paper presents a new clutter rejection technique using PCA features and stochastic features of clutters and targets. PCA features are obtained from Euclidian distances using which potential targets are projected to reduced eigenspace selected from target eigenvectors. CV is used for calculating stochastic features of edges in targets and clutters images. To distinguish between target and clutter, LDA (Linear Discriminant Analysis) is applied. The experimental results show that the proposed algorithm accurately classify clutters with a low false rate compared to PCA method or CV method

• Korean journal of applied entomology
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• 제53권2호
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• pp.103-110
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• 2014

The efficacy of mating disruptors MD-IKR (Isomate-KR) and MD-CR (Confuser-R) for controlling Grapholita molesta (Busck) and Carposina sasakii (Matsumura) was evaluated in non-chemical or organic apple orchards. For G. molesta, the efficacy of MD-IKR and MD-CR in Chilgok was 99.8 and 99.8% (2011) and 92.4 and 96.7% (2012), respectively, showing different mating reduction values. The efficacy of MD-IKR and MD-CR in Cheongsong was 99.1 and 92.8% (2011) and 99.6 and 99.6% (2012), respectively. For C. sasakii, the efficacy of MD-IKR and MD-CR was 100% at Chilgok and Cheongsong in 2011 and 2012. Evaluation of the shooting and fruit damage rates showed that the efficacy of the mating disruptors in controlling G. molesta and C. sasakii was better than that of the non-control. However, there was no significant control difference between using the mating disruptors and the conventional control. No difference in the efficacy of the two mating disruptors was observed. We believe that MD-IKR could be an alternative to MD-CR.

• Journal of Mushroom
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• 제10권1호
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• pp.3-8
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• 2012

In this study, to produce Flammulina velutipes mushroom liquid spawn efficiently and effectively the effects of explosive aeration (supplying air with tiny bubbles) of the liquid culture medium on carbon dioxide concentration and residual sugar content in the medium on carbon dioxide concentration and residual sugar contentin the medium were measured. Carbon dioxide concentrations were measured at the outlet of the incubator. On the third day the explosive aeration greatly increased mycelial growth of the liquid spawn, and carbon dioxide concentration also greatly increased but decreased after 5 days. Free sugar contents in the liquid culture consistantly decreased up to 7 days and thereafter was not detected. The weight of the mycelia were maintained similar levels after 3 days. Total nitrogen content in the liquid medium constantly decreased during the 11days of explosive aeration. The content of free sugars in 7 days of culture was the lowest level, thus the inoculum incubated for 6~7 days was thought to be the most effective. Carbon dioxide concentration measurement at the outlet of the container during the liquid spawn incubation required low cost but was efficient to estimate the degree of mycelial growth to be used as a simple indicator.

• Journal of Mushroom
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• 제10권1호
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• pp.15-20
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• 2012

Ventilation effects of bottles($1,100m{\ell}$) for culturing Flammulina velutipes on its mycelial growth and mushroom production were investigated. The degree of ventilation were controlled with hole positions, upper and under, and hole sizes in the bottle lids. The ventilation effects were measured with the contents of carbon dioxide, free sugars, chitin, moisture in the bottles and with the amount of produced mushrooms from the bottles. Carbon dioxide concentrations within the culturing bottles at exponential mycelial growth period vertex were relatively high in the bottles with lids without both a sponge and an aeration hole, and in those with a smaller hole. Free sugar contents in the mycelia were the highest in those with a 47mm hole on both sides, and in those with 26~33mm holes only underside. Chitin content was the highest in those with a 26mm hole only underside. On the other hand, the lids with 42mm~47mm holes on the both sides greatly lost water and decreased the mushroom production. In conclusion, the most efficient ventilation hole sizes on the lids for bottle($1,100m{\ell}$) cultivation of Flammulina velutipes using $1,100m{\ell}$ polypropylene bottle were 19mm on both sides of the lid and 26mm on only underside. They produced more mushrooms than the control by 6~9 %.

• The Journal of Korean Association of Computer Education
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• 제8권4호
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• pp.79-86
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• 2005

Educational objectives are the most important items because not only they must be decided first in the circulation process of curriculum but also they should be the basis for the process of curriculum. The establishment of objectives is very important because it can determine success or failure of computer education. In this paper, questionnaire research is conducted and analyzed for male students of middle schools in Chungbuk province, Korea to determine that it's reasonable for computer games to be perceived negatively. The results of the research are as follows. 48% of them are represented to play computer game almost everyday, and 8% to play it scarcely. So most of students are enjoying it regardless of their grade. If students whose grades are ranked upper level start playing games, they're tend to quit it within 1 or 2 hours. And they enjoy role-playing or simulation game. In general, they do not play action games. If their grades are ranked lower level start playing games, a lot of them continue to play longer than 3 hours like game addict. And in this level, there exist students who barely play games. In middle level, there are no apparent characteristics. According to the result of the research, there is no correlation between frequency of playing games and academic achievements. So it's no problem to relieve worries if one often play computer games, his or her grade will get down. In case that the grades of students are ranked upper level, however, there are few who continue to play games longer than 3 hours.

• Journal of the Korean Chemical Society
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• 제64권2호
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• pp.84-98
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• 2020

In this study, we analyzed the influences of lecture design using CoRe upon the professor's teaching professionalism in the aspects of pedagogical content knowledge (PCK). The participants are three professors from the college of science-engineering located in Chungcheong-do. After collecting their syllabi, we observed their lecture and conducted the orientation. Afterward, we collected the CoRes which they prepared before the lecture. Then we observed their lecture and conducted semi-structured interviews. This process was carried out twice. We analyzed their syllabi, CoRes, videotaped lectures, field notes, the teaching materials, and interview transcripts. The results revealed that professors not only clarified the learning objectives and the characteristics of students but also reflected them in the lecture. In addition, they established the teaching strategies according to the characteristics of contents in the unit. As they recognized the necessity of understanding students' achievement, they selected the assessment method and applied it in the lecture. In some cases, however, they lacked presenting learning objectives specifically and explained students' misconceptions without inducing new concepts. They also presented a shortage of considering students' prior knowledge. They lacked providing students with an opportunity to participate in lectures, and their assessment method was not effective. Based on the results, we discussed implications to improve teaching professionalism using CoRe.

• Journal of the Korea Society of Computer and Information
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• 제17권7호
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• pp.129-138
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• 2012

Despite of increasing pressure of opening to free for medical market by an advanced nation and increasing number of portal websites for health information, there have been few studies on the issues of enhancing the service quality of health information websites. The survey exhibited that the quality factors mainly affect significant and satisfied level of health information websites were usability, information structure and contents, interaction, and revisiting. This study was conducted to develop the consumer-focused quality factor of health information websites using WebQual model which was valued at efficient evaluation tool for this information system The methods taken in this research was to survey quality factors for significant and satisfied level of 323 consumers live in Chungcheong-do which is based on 48 questionnaires of WebQual quality factors. Our research extracted quality factors, usability, information quality (information structure and contents), interaction (personalisation and privacy) about view of criticality and satisfaction and relevant degree of revisiting. The research results presented in the paper will provide an efficient way of installing healthcare websites, moreover stone toward a marketing strategy for the consumer focus website provides under fierce competition.