• Journal of Microbiology
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• 제45권5호
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• pp.460-465
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• 2007

This study was carried out in order to investigate the potential of using plant oils derived from Leptospermum petersonii Bailey and Syzygium aromaticum L. Merr. Et Perry as natural antifungal agents. The antifungal effects of essential oils at concentrations of 0.05, 0.1, 0.15, and 0.2 mg/ml on the dermatophytes Microsporum canis (KCTC 6591), Trichophyton mentagrophytes (KCTC 6077), Trichophyton rubrum (KCCM 60443), Epidermophyton floccosum (KCCM 11667), and Microsporum gypseum were evaluated using the agar diffusion method. The major constituents of the active fraction against the dermatophytes were identified by gas chromatography-mass spectrometry and high-performance liquid chromatography analysis. The antifungal activities of S. aromaticum oil (clove oil) against the dermatophytes tested were highest at a concentration of 0.2mg/ml, with an effectiveness of more than 60%. Hyphal growth was completely inhibited in T. mentagrophytes, T. rubrum, and M. gypseum by treatment with clove oil at a concentration of 0.2 mg/ml. Eugenol was the most effective antifungal constituent of clove oil against the dermatophytes T. mentagrophytes and M. canis. Morphological changes in the hyphae of T. mentagrophytes, such as damage to the cell wall and cell membrane and the expansion of the endoplasmic reticulum, after treatment with 0.11 mg/ml eugenol were observed by transmission electron microscopy (TEM). At a concentration of 0.2 mg/ml, L. petersonii oil (LPO) was more than 90% effective against all of the dermatophytes tested, with the exception of T. rubrum. Geranial was determined to be the most active antifungal constituent of L. petersonii oil. Taken together, the results of this study demonstrate that clove and tea tree oils exhibited significant antifungal activities against the dermatophytes tested in this study.

• The Korean Journal of Food And Nutrition
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• 제21권2호
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• pp.227-242
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• 2008

This study was conducted to construct database of Korean local foods, and to investigate the total kinds of local foods using a traditional Korean food classification system. A total of 2,137 kinds of local foods were investigated. The results for 10 local provinces are as follows. Seoul had a total of 116 kinds of local foods that were investigated including 13 main dishes, 41 side dishes, 22 kinds of ttdok lyou, 20 kinds of hangwa lyou, and 19 kinds of emuchong lyou. The Gyeonggi local foods totaled 209 kinds: 37 kind main dishes, 130 kind side dishes, 26 kinds of ttdok lyou, 7 kinds of hangwa lyou, and 9 kinds of emuchong lyou. The Gangwon local foods included a total of 198 kinds: 37 main dishes, 111 side dishes, 35 kinds of tteok lyou, 8 kinds of hangwa lyou, and 7 kinds of eumchong lyou. The Chungbuk local foods included a total of 123 kinds: 19 main dishes, 68 side dishes, 17 kinds of tteok lyou, 14 kinds of hangwa lyou, and 5 kinds of eumchong lyou. The Chungnam local foods totaled 185 kinds: 26 main dishes, 124, side dishes, 16 kinds of tteok lyou, 14 kinds of hangwa lyou, and 5 kinds of eumchong lyou. The Gyongbuk local foods investigated included 314 kinds: 52 main dishes, 170 side dishes, 52 kinds of tteok lyou, 21 kinds of hangwa lyoll, and 19 kinds of eumchong lyou. The Gyongnam local foods totaled 342 kinds: 63 main dishes, 234 side dishes, 16 kinds of tteoks lyou, 15 kinds of hangwa lyou and 14 kinds of eumchong lyou. The Jeonbuk local foods totaled 146 kinds: 25 main dishes, 85 side dishes, 18 kinds of tteok lyou, 14 kinds of hangwa lyou, and 4 kinds of eumchong lyou, The Jeonnam local foods totaled 309 kinds: 32 main dish, 207 side dishes,20 kinds of tteok lyou, 27 kinds of hangwa lyou, and 23 kinds of eumchong lyou. Finally, the Jeju local foods totaled 195 kinds: 36 main dishes, 123 side dishes, 19 kinds of tteok lyou, 10 kinds of hangwa lyou and 7 kinds of eumchong lyou.

• Korean Journal of Food Science and Technology
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• 제28권3호
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• pp.580-586
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• 1996

Gelatinization behavior and morphology of epichlorohydrin-crosslinked potato starches (XPs) were investigated. Native potato starch showed a very steep single stage swlling pattern, but crosslinked starches showed various patterns with the degree of crosslinking. Swelling power, solubility and light transmittance were reduced drastically as the degree of crosslinking increased. Brabender initial pasting temperature and peak temperature of crosslinked starches increased because the crosslinking reinforces the intermolecular net work of the starches. Although the swelling of the potato starch granule was inhibited by crosslinking as compared to that of the native one, Brabender peak viscosities (6.5% w/v, db) were on the order of 2,500 units for the native potato starch, 3,700 for the XP with 2.300 anhydroglucose units per crosslinking (AGU/CL) and 3,400 for the XP with 2,100 AGU/CL, due to the decreased breakdown of the swollen granule resulting from the resistance to heat and shear. The XP with 1,900 AGU/CL, however, did not show the peak viscosity and the viscosity was on the order of 500 units because of the excessive unhibition of the swelling. Unlike the native potato starch, 6.5%(m/v, db) pastes of the crosslinked potato starches could form gels, which could be predicted from the Brabender setback and consistency index. When the degree of crosslinking is low, random contraction and radial swelling of the granule was possible. As the degree of crosslinking increased, morphological change became similar to the single dimensional tangential swelling observed from the lenticular wheat starch. These morphological change during heating in excess water explained the gelatinization behaviors of crosslinked starches tested.

• Korean Journal of Food Science and Technology
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• 제37권6호
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• pp.889-892
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• 2005

Standardized method based on extraction, filtration, and gas chromatography (GC) was developed far propylene glycol analysis to set hygienic norm of safety measure for foods under governmental control. Various columns were tested fur propylene glycol analysis by GC with flame ionization detector. Known amount of propylene glycol was spiked into wheat flour dough and analyzed by developed method. Results showed 101.60% recovery rate for propylene glycol with HP-5 column. Reproducibility test of standards recorded 0.30 for standard variation and 0,42% for relative variation. Using analytical method established, contents of propylene glycol in more than hundred different foods were monitored. Propylene glycol was detected in most foods, indicating propylene glycol is not only commonly added during food preparation, but also is contained naturally in food.

• Journal of Korea Water Resources Association
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• 제48권10호
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• pp.857-868
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• 2015

Hydraulic and water quality models with high reliability are necessary for the efficient management of water quality in the reservoir. The model capacity can be demonstrated by the application for the various hydrological conditions. CE-QUAL-W2 model is laterally averaged two-dimensional hydraulic and water quality model. The W2 model, which is suitable for the narrow reservoir like the Jinyang reservoir as compared with the depth and length of waterbody, has been frequently used by many researchers. Namgang watershed is expected to increase the water demand. In this study, the W2 model is validated under two different hydrological conditions; wet year (2011) and normal year (2009). Using hydrological and water quality condition for calibration, 2011, the effect of water intake increase was simulated. The simulation results showed that the increase of water intake led to increase the concentrations in total nitrogen, total phosphorus and Chlorophyll-${\alpha}$ concentration. Especially the concentration increase was appeared during the dry season in each of up to 62.53% (Total nitrogen), 39.07% (Total phosphorus) and 232.19% (Chlorophyll-${\alpha}$). The changes of chlorophyll-${\alpha}$ is similar to those of total phosphorus concentration.

• Korean Journal of Medicinal Crop Science
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• 제5권4호
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• pp.266-275
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• 1997

Anemarrhena asphodeloides BUNGE is one of important medicinal crops, which has been collected or/and cultivated for its rhizomes. The main medicinal ingredient of the A. asphodeloides Bunge rhizomes is a saponin, which is known to have medical values for diaphoresis, sedatives and biuresis. However, studies on cultural methods and breeding works on this plant have not been made in detail. To increase productivity and to improve quality of crops, it is important to collect cultivated and wild lines, to classify them based on morphological and genetic characteristics, and to select superior pure lines at first. Therefore, total 20 A. asphodeloides lines collected were cultivated at the fieldof Chungnam Provincial Administration of Rural Development in 1995 to study the agronomic characteristics and to classify them based on morphological characteristics. Characteristics related with reproductive organ such as the number of spikes per plant and peduncle length showed greater variations than vegetative organrelated characteristics such as leaf length and rhizome length based on the coefficient of variation. Vigorous shoot growth resulted in better development of reproductive organs such as peduncle length and number of spikes per plant. However, the development of spikes was negatively correlated with chlorophyll content. Characteristics of underground parts were more significantly correlated with spike characteristics than aerial part characteristics. A. asphodeloides tested in this study were classified into 2 groups (group A and group B) based on centroid linkage cluster analysis. Group A showed more vigorous shoot growth with more leaves and spikes per plant, longer peduncle length, thicker peduncle diameter and higher shoot dry weight than group B. Group A could be further classified into 2 sub-groups based on leaf size, spike length and peduncle diameter, while group B also could be classified based on number of leaves, number of spikes and shoot dry weight.

• Korean Journal of Medicinal Crop Science
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• 제10권1호
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• pp.29-36
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• 2002

Epimedium koreanum Nakai(EKN) has traditionally been well-known as a herbal medicine for the promotion of stamina and the improving sexual activity of human in oriental countries including Korea and China. The aim of this study was to investigate the effect of dietary supplementation of EKN water-extract(0.025%,w/v) on age-related change of xenobiotic metabolizing system in rat liver. Long-term supply of the EKN extract to rats did not induce any discernible signs. However, the mass of liver and kidney were slightly increased by the dietary supplementation. Level of cytochrome P450, NADPH cytochrome P450 reductase, P450 dependent ethoxycoumarin O-deethylase benzphetamine N-demethylase and glutathione-S-transferase in liver were decreased with age, but, these activities in 24 months of age were declined more in rats supplied with EKN extract. Level of cytochrome b5 and NADH-cytochrome b5 reductase were also decreased with age, however, there was no significant difference between with and without EKN extract. These results indicate that long-term supplementation of EKN extract to rats from weaning to 24 months may be a burden on the liver function in old age, and may aggregate the decline of xenobiotic metabolizing enzymes activities in old age.

• Journal of Microbiology
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• 제44권2호
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• pp.192-199
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• 2006

Pseudomonas putida KL47 is a natural isolate that assimilates benzene, 1-alkylbenzene $(C_1-C_4)$, biphenyl, p-cumate, and p-cymene. The genetic background of strain KL47 underlying the broad range of growth substrates was examined. It was found that the cym and cmt operons are constitutively expressed due to a lack of the cymR gene, and the tod operon is still inducible by toluene and biphenyl. The entire array of gene clusters responsible for the catabolism of toluene and p-cymene/p-cumate has been cloned in a cosmid vector, pLAFR3, and were named pEK6 and pEK27, respectively. The two inserts overlap one another and the nucleotide sequence (42,505 bp) comprising the cym, cmt, and tod operons and its flanking genes in KL47 are almost identical (>99 %) to those of P. putida F1. In the cloned DNA fragment, two genes with unknown functions, labeled cymZ and cmtR, were newly identified and show high sequence homology to dienelactone hydrolase and CymR proteins, respectively. The cmtR gene was identified in the place of the cmtI gene of previous annotation. Western blot analysis showed that, in strains F1 and KL47, the todT gene is not expressed during growth on Luria Bertani medium. In minimal basal salt medium, expression of the todT gene is inducible by toluene, but not by biphenyl in strain F1; however, it is constantly expressed in strain KL47, indicating that high levels of expression of the todST genes with one amino acid substitution in TodS might provide strain KL47 with a means of adaptation of the tod catabolic operon to various aromatic hydrocarbons.

• Journal of Microbiology
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• 제44권3호
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• pp.301-310
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• 2006

Two pathways of ammonium assimilation and glutamate biosynthesis have been identified in microorganisms. One pathway involves the NADP-linked glutamate dehydrogenase, which catalyzes the amination of 2-oxoglutarate to form glutamate. An alternative pathway involves the combined activities of glutamine synthetase, which aminates glutamate to form glutamine, and glutamate synthase, which transfers the amide group of glutamine to 2-oxoglutarate to yield two molecules of glutamate. We have cloned the large subunit of the glutamate synthase (GOGAT) from Salmonella typhimurium by screening the expression of GOGAT and complementing the gene in E. coli GOGAT large subunit-deficient mutants. Three positive clones (named pUC19C12, pUC19C13 and pUC19C15) contained identical Sau3AI fragments, as determined by restriction mapping and Southern hybridization, and expressed GOGAT efficiently and constitutively using its own promoter in the heterologous host. The coding region expressed in Escherichia coli was about 170 kDa on SDS-PAGE. This gene spans 4,732 bases, contains an open reading frame of 4,458 nucleotides, and encodes a mature protein of 1,486 amino acid residues (Mr =166,208). The EMN-binding domain of GOGAT contains 12 glycine residues, and the 3Fe-4S cluster has 3 cysteine residues. The comparison of the translated amino acid sequence of the Salmonella GOGAT with sequences from other bacteria such as Escherichia coli, Salmonella enterica, Shigella flexneri, Yersinia pestis, Vibrio vulnificus and Pseudomonas aeruginosa shows sequence identity between 87 and 95%.

• Korean Journal of Human Ecology
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• 제2권1호
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• pp.45-51
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• 1993

The purpose of this study is to provide some information for the proper education on clothing wearing habits so that the education may be performed in the direction of social needs according to the social change and in the direction of the needs and interest of the high school girl students in their adolescence by being conscious of the influence of clothing on the high school girl students. I have used questionaire as an instrument of measurement. The length of the time of survey is from February 11, 1991 to February 28. The subjects are 544 first grade girl students of general high schools in Cheong-Ju, Choong-Ju, and Je-Cheon. The analysis of the data includes frequency, percentage, average person's Correlation Program, t-test, ANOVA, Scheffe's test. The conclusion of this study is as follows: The first, it is shown that (l)students's interest in the design and fashion of clothing is significantly related to the socio-anthropological and socio-psychological factors such and the education level of their fathers, the education level of their mothers, monthly income of their homes, the clothing habits of their home economics teachers, the subjects which they are interested in, their average scholoary achievements, their religious background, and whether they have boy friends or not, etd. (2)students' interest in purchasing of clothing is significantly related to the socio-anthropological and socio-psychological factors such as the education level of their fathers, the education level of their mothers, monthly income of their homes, the clothing habits of their home economics teachers, the subjects which they are interested in, their average scholoary achievements, and whether they have boy friends or not, etc. (3)students' interest in the social-psyclological aspect is significantly related to the socio-anthropological and socio-psychological factors such as their living area, the clothing habits of their home economics teachers, etc. (4)students' interest in the clothing construction is significantly related to only the clothing habits of their home economics teachersl. (5)students' interest in the clothing management is significantly related to the socio-anthropological and socio-psychological factors such as the education level of their parents, the age of their mothers, their religious background, and their sisters. The second, as for the interest of the high school girl students in clothing, it has been shown that those who wear school uniforms tend to have more interest than those who wear free-choice clothes in such areas as shopping, the construction, and management. As for the other two areas, that is, disign and fashion, and the interest in socio-psychological dependence there has been little meaningful difference between the two groups, while the uniform group has shown more interest than the other group. The third, the interest of hish school students in clothing is considered to be most seriously influenced by their socio-psychological dependence out of five areas of interest. It has been shown that the proportion of the content dealing with clothing wearing life in the high school home economics texbooks in relatively small, especially in such areas as socio-psychological dependence, shopping, management, and design and fashion except in construcion.