• The Korean Journal of Physiology and Pharmacology
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• v.27 no.4
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• pp.383-398
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• 2023

Dihydroaustrasulfone alcohol (DA), the synthetic precursor of a natural compound (austrasulfone) isolated from the coral species Cladiella australis, has shown cytotoxic effects against cancer cells. However, it is unknown whether DA has antitumor effects on nasopharyngeal carcinoma (NPC). In this study, we determined the antitumor effects of DA and investigated its mechanism of action on human NPC cells. The MTT assay was used to determine the cytotoxic effect of DA. Subsequently, apoptosis and reactive oxygen species (ROS) analyses were performed by using flow cytometry. Apoptotic and PI3K/AKT pathway-related protein expression was determined using Western blotting. We found that DA significantly reduced the viability of NPC-39 cells and determined that apoptosis was involved in DA-induced cell death. The activity of caspase-9, caspase-8, caspase-3, and PARP induced by DA suggested caspase-mediated apoptosis in DA-treated NPC-39 cells. Apoptosis-associated proteins (DR4, DR5, FAS) in extrinsic pathways were also elevated by DA. The enhanced expression of proapoptotic Bax and decreased expression of antiapoptotic BCL-2 suggested that DA mediated mitochondrial apoptosis. DA reduced the expression of pPI3K and p-AKT in NPC-39 cells. DA also reduced apoptosis after introducing an active AKT cDNA, indicating that DA could block the PI3K/AKT pathway from being activated. DA increased intracellular ROS, but N-acetylcysteine (NAC), a ROS scavenger, reduced DA-induced cytotoxicity. NAC also reversed the chances in pPI3K/AKT expression and reduced DA-induced apoptosis. These findings suggest that ROS-mediates DA-induced apoptosis and PI3K/AKT signaling inactivation in human NPC cells.

• Proceedings of the KIEE Conference
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• 2008.04c
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• pp.119-121
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• 2008

This paper is proposed a high Performance speed control of the synchronous reluctance motor through the SV-PWM(Space Vector Pulse Width Modulation) of FAM-PI(Fuzzy Adaptive Mechanism-PI). SV-PWM is controlled using FAM-PI control. SV-PWM can be maximum used maximum do link voltage and is excellent control method due to characteristic to reducing harmonic more than others. Fuzzy control has a advantage which can be robustly controlled. FAM-PI controller is changed fixed gain of PI controller using fuzzy adaptive mechanism(FAM) to match operating condition. The results on a speed controller of IPMSM are presented to show the effectiveness of the proposed gain tuner. And this controller is better than the fixed gains one in terms of robustness, even under great variations of operating conditions and load disturbance.

• Proceedings of the KIEE Conference
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• 2007.04c
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• pp.91-93
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• 2007

This paper presents multi-PI controller of IPMSM drive using fuzzy and neural-network. In general, PI controller in computer numerically controlled machine process fixed gain. To increase the robustness, fred gain PI controller, Multi-PI controller proposes a new method based fuzzy and neural-network. Multi-PI controller is developed to minimize overshoot and settling time following sudden parameter changes such as speed, load torque, inertia, rotor resistance and self inductance. The results on a speed controller of IPMSM are presented to show the effectiveness of the proposed gain tuner. And this controller is better than the fixed gains one in terms of robustness, even under great variations of operating conditions and load disturbance.

• Proceedings of the KIEE Conference
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• 2005.04a
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• pp.191-193
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• 2005

This paper presents Hybrid PI controller of IPMSM drive using fuzzy adaptive mechanism(FAM) control. To increase the robustness, fixed gam PI controller, Hybrid PI controller proposes a new method based self tuning PI controller. Hybrid PI controller is developed to minimize overshoot and settling time following sudden parameter changes such as speed, load torque, inertia, rotor resistance and self inductance. The results on a speed controller of IPMSM are presented to show the effectiveness of the proposed gain tuner. And this controller is better than the fixed gains one in terms of robustness, even under great variations of operating conditions and load disturbance.

• Journal of the Korean Institute of Intelligent Systems
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• v.25 no.6
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• pp.565-570
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• 2015

In this paper, we implemented the smart door lock system that control remotely devices using the concept of internet of things. Internet of things is intelligent system that can help devices to communicate with people and devices. And recently internet of things is getting attention because of advance of hardware technology and big data. The smart doorlock system based on internet of things used raspberry pi, sensor and doorlock. Using the smart phone, doorlock can be controlled from the raspberry pi server. And the user can identify some people that is in front of doorlock. also user can check around of doorlock in realtime using the raspberry pi camera.

• Proceedings of the KIPE Conference
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• 2011.11a
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• pp.233-234
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• 2011

본 논문에서는 IPMSM의 약자속 제어 영역에서 토크제어를 하기위한 전류제어기를 PI-IP 혼합제어기로 구성하였다. 약자속 제어 영역에서 토크제어 시 d축과 q축의 인덕턴스 차이로 인해 전류제어 응답의 차이가 발생하여 전압여유분 확보에 문제점이 발생한다. 이러한 문제점을 개선하고자 PI-IP 혼합제어기를 적용하여 약자속 영역에서의 d축과 q축의 전류 제어기의 응답특성을 달리하여 토크제어 시 과도상태에서 발생하는 문제점을 개선하였다.

• Proceedings of the KIPE Conference
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• 2009.11a
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• pp.93-95
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• 2009

The synchronous PI current controller has been widely used for the high performance PM synchronous drives and generally implemented using a digital signal processor. This paper describes the control characteristics of the digital PI current controller for PM synchronous drives. The stability of the current control system considering the sampling time is investigated and the effects of the mismatched decoupling terms in the discrete-time domain are also discussed. The simulation results are provided to verify the theoretic results.

• Proceedings of the KIEE Conference
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• 1992.07b
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• pp.864-867
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• 1992

This paper describes optimum conditions for depositing Langumuir Blodgett(LB) films. $\pi$-A isotherm characteristics were studied with the following species, $C_{18}$-Quinolium(TCNQ), $C_{22}$-Quinolium(TCNQ), Azobenzene, Arachidic Acid, by varing length of alkyl chains, temperacture, pH, spreading amount, barrier moving speed and etc. We have observed the change of $\pi$-A isotherm depending on the above variations. From there we investigate the depositing conditions of LB films for each material.

• Proceedings of the KIPE Conference
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• 2019.11a
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• pp.144-145
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• 2019

This paper proposes a design and analysis for a PI-IP hybrid voltage controller with a combination of PI and IP voltage controller for stable voltage control of DC bus voltage, Transient characteristic of DC bus voltage is improved by designed setting variable value and control method in the variable load and power generation conditions.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.24 no.4
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• pp.33-42
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• 2010

The induction motor drive applied to field oriented control is widely used in industry applications. However, it is deceased performance and authenticity by saturation, temperature changing, disturbance and parameters changing because modeling of induction motor is nonlinear and complex. In order to control variable speed operation, conventional PI-like controllers are commonly used. These controllers provide limited good performance over a wide range of operation, even under ideal field oriented conditions. This paper proposes self tuning PI controller based on fuzzy-neural network(FNN)-PI controller that is implemented using fuzzy control, neural network, and adaptive fuzzy controller(AFC). Also, this paper proposes estimation of speed using ANN. The proposed control algorithm is applied to induction motor drive system using FNN-PI, AFC and ANN controller. Also, this paper proposes the anlysis results to verify the effectiveness of controller.