• Journal of Veterinary Clinics
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• v.33 no.3
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• pp.165-167
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• 2016

A case of rectal prolapses in a one-year-old intact male mixed-breed dog, weighing 6.8 kg was presented with one-week history of protruded tubular pink mass through the anus along with mucosal necrosis. The prolapse was non-reducible, so rectal amputation and anal purse-string suture was performed. Prolapse recurred 9 days after the surgery, purse-string suture and conservative treatment were attempted with no-good results. Laparoscopic-assisted incisional colopexy technique was applied to treat the recurrent rectal prolapse, but failed 6 days after surgery. This was followed by non-incisional colopexy technique, which prevented recurrence during the 3 months of follow-up period. Despite the episode of recurrence, the laparoscopic-assisted colopexy technique treated rectal prolapse successfully. This is the first report in Korea, which describes laparoscopic-assisted colopexy in the dog.

• Journal of Veterinary Clinics
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• v.28 no.4
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• pp.394-398
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• 2011

Mesenchymal stem cells (MSC) are pluripotent cells that can be found in umbilical cord blood from new borne babies as well as placenta, bone marrow, adipose tissue, amniotic fluid, muscle, et al. MSC are capable of renewing themselves without differentiation in long-term culture, also can be differentiated into various tissues under specific condition. Formulating a cryopreservation protocol for the MSC is required because these cells cannot survive for long periods under in vitro culture conditions and a new formulation of harmless cryoprotectant is needed for the direct injection of MSC into patients. The undifferentiated MSC were frozen with a vitrification solution of 40% ethylene glycol, 20% Ficoll-70 and 0.3M sucrose. The survival rate after thawing and their proliferation rate were examined and compared with slow rate cooling methods using dimethylsulfoxide (DMSO). The vitrification method showed high survival rate after thawing and proliferation capacity comparable to DMSO. It can be suggested that ultra-rapid cooling method by vitrification is reliable methods for long term preservation of MSC and the vitrification solution with ethylene glycol, Ficoll-70 and sucrose will be more beneficially used for direct transplantation of MSC into patients than DMSO solution.

• Journal of Microbiology and Biotechnology
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• v.17 no.3
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• pp.511-515
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• 2007

White-spot syndrome virus (WSSV) is a devastating, infectious virus affecting shrimp. Although sensitive techniques involving PCR have been developed to assist farmers in screening shrimp (brood stock) for WSSV prior to stocking ponds, such practices have not yet been applied in Korea. Despite the rationality of implementing screening, there has been some doubt as to whether the stocking of WSSV-PCR-negative fly epidemiologically decreases white-spot disease outbreaks. Here, we report a retrospective analysis of data from shrimp farms in the western coast of Korea where WSSV-PCR-negative brood stocks were used to stock rearing ponds. A total of 366 shrimp from Heuksan Island were sampled for WSSV with PCR. Of the tested shrimp, 7.2% (28 brood stocks) were identified as WSSV positive; only WSSV-PCR-negative shrimp were used for brood stocks. Total unit production (final shrimp production/ the area of the ponds) was higher, at 1.96, in ponds where WSSV-PCR-negative shrimp were used, as compared with 1.02 in other ponds in Korea in 2004. This retrospective analysis of WSSV in Korea may be useful to the shrimp aquaculture industry, suggesting a testable hypothesis that may contribute to the eventual control of WSSV outbreaks.

• Journal of Biosystems Engineering
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• v.39 no.4
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• pp.324-329
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• 2014

Purpose: The finite element method (FEM) is advantageous because it can save time and cost by reducing the number of samples and experiments in the effort to identify design factors. In computational problem-solving it is necessary that the exact material properties are input for achieving a reliable analysis. However, in the case of fiber boards, it is difficult to measure their cross-directional material properties because of their small thickness. In previous research studies, the Poisson's ratio was measured by analyzing ultrasonic wave velocities. Recently, the Poisson's ratio was measured using a high-speed digital camera. In this study, we measured the transverse strain of a fiber board and calculated its Poisson's ratio using a high-speed digital camera in order to apply these estimates to a FEM analysis of a fiber board, a corrugated board, and a corrugated box. Methods: Three different fiber board samples were used in a uniaxial tensile test. The longitudinal strain was measured using the Universal Testing Machine. The transverse strain was measured using an image processing method. To calculate the transverse strain, we acquired images of the fiber board before the test onset and before the fracture occurred. Acquired images were processed using the image processing program MATLAB. After the images were converted from color to binary, we calculated the width of the fiber board. Results: The calculated Poisson's ratio ranged between 0.2968-0.4425 (Machine direction, MD) and 0.1619-0.1751 (Cross machine direction, CD). Conclusions: This study demonstrates that measurement of the transverse properties of a fiber board is possible using image processing methods. Correspondingly, these processing methods could be used to measure material properties that are difficult to measure using conventional measuring methodologies that employ strain gauge extensometers.

• Journal of Korea Multimedia Society
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• v.20 no.12
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• pp.1960-1969
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• 2017

There have been ongoing researches to identify and analyze the patterns of electric power IoT data inside sensor nodes to supplement the stable supply of power and the efficiency of energy consumption. This study set out to propose an analysis process for electric power IoT data with the K-means algorithm, which is an unsupervised learning technique rather than a supervised one. There are a couple of problems with the old K-means algorithm, and one of them is the selection of cluster number K in a heuristic or random method. That approach is proper for the age of standardized data. The investigator proposed an analysis process of selecting an automated cluster number K through principal component analysis and the space division of normal distribution and incorporated it into electric power IoT data. The performance evaluation results show that it recorded a higher level of performance than the old algorithm in the cluster classification and analysis of pitches and rolls included in the communication bodies of utility poles.

• Journal of Veterinary Clinics
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• v.28 no.1
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• pp.149-153
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• 2011

In this report, laparoscopic ovariohysterectomy (LOHE) was performed on two, healthy, intact female dogs. Three ports, umbilical port for placing the laparoscope and the left and right paramedian instrument ports were placed into the abdominal cavity. First, in order to isolate the right ovary, the vascular pedicle was coagulated by activating the universal bipolar forceps. Then, the pedicle and suspensory ligament were transected by the bipolar electrocauterization scissors. In the same manner, the left ovary was isolated. The uterine body and the uterine arteries were coagulated bilaterally and transected just rostral to the cervix. The isolated ovaries and uterus were exteriorized through the left paramedian instrument port, which was extended by approximately 1 cm to allow for tissue removal. The skin and subcutaneous tissue were closed in a routine manner. Surgical times for LOHE in two dogs were 35, 40 min respectively. Total length of abdominal incision was shorter than 3 cm in two dogs. No clinical complications related to the LOHE were observed during 1.5 years after surgery. The LOHE is an alternative surgical technique that deserves further investigation.

• Journal of Veterinary Clinics
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• v.35 no.4
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• pp.137-140
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• 2018

A five-month-old toy poodle was referred for treatment of bilateral mandibular fractures. Preoperative skull and dental radiographs showed rostral mandibular fractures and permanent teeth that had not yet erupted. In addition to providing stability for the healing of fractures, it is very important to focus on restoring normal occlusion. The mandibular fractures were repaired using interdental wiring in a dentate area where the deciduous canines and fourth premolars were stable and could be used to anchor the interdental fixation. In the absence of an erupted permanent mandibular first molar, the modified Risdon wiring was performed using the deciduous mandibular fourth premolars as anchor teeth. An intraoral splint was placed on the mandibular teeth using self-curing composite resin. Five months after surgery, the patient had experienced no complications, and the permanent teeth had erupted normally. In this case, which involves a small-breed puppy with deciduous dentition, treatment with a wire-reinforced interdental splint using intact deciduous fourth premolar teeth as anchor teeth can be considered as a suitable method for rostral mandibular fracture restoration.

• Journal of Microbiology and Biotechnology
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• v.18 no.12
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• pp.1984-1989
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• 2008

Surfactin is a natural biosurfactant derived from Bacillus subtilis and has various biological activities such as anticancer, antiplatelet, and anti-inflammatory effects. In this study, the inhibitory mechanism of surfactin in NO production from macrophages was examined. Surfactin down regulated LPS-induced NO production in RAW264.7 cells and primary macrophages with $IC_{50}$ values of 31.6 and $22.4{\mu}M$, respectively. Immunoblotting analysis showed that surfactin strongly blocked the phosphorylation of IKK and $l{\kappa}B{\alpha}$ and the nuclear translocation of $NF-{\kappa}B$ (p65). Therefore, these data suggest that surfactin may act as a bacterium-derived anti-inflammatory agent with anti-$NF-{\kappa}B$ activity.

• Food Science and Biotechnology
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• v.14 no.6
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• pp.752-757
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• 2005

Changes in milk quality during storage of extended shelf life milk (ESL milk) and non-ESL milk were evaluated. No significant differences were observed between ESL and typical ultra high temperature-treated (UHT) milk in physicochemical properties including non-casein nitrogen (NCN) content, whey protein nitrogen index (WPNI), and L-ascorbic acid content. Low temperature and long time-treated milk (LTLT milk) had significantly higher NCN content and WPNI than those of UHT milk. In terms of microbial quality, yeast, molds, coliforms, and other bacteria were not detected in ESL milk during entire storage (21 days after expiration date) period at 4 and $25^{\circ}C$, while LTLT milk was more susceptible to microbial infection. Rats fed ESL milk resulted in significantly higher body weight, average daily gain, and feed efficiency than those given UHT milk. These results suggest ESL milk maintains better microbial quality than typical UHT milk, particularly during storage under extended refrigeration and at high temperature.

• Journal of Veterinary Clinics
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• v.33 no.1
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• pp.30-33
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• 2016

It is difficult to detect and diagnose a mass at the tongue base, particularly if the mass is located under normal appearing mucosa. Computed tomography (CT), particularly post-contrast images, is useful to evaluate a tongue mass and adjacent structures including cervical lymph nodes. Nevertheless, a definitive diagnosis of a mass is obtained by histopathological examination. In this case, we describe a mass arising from the tongue body to the root in a dog. The patient was referred with ptyalism and an eating disorder. An oral examination was performed, and decreased tongue motility was observed. CT scanning was performed, and a lingual mass was detected. A lingual mass sample was collected by biopsy at the postmortem 2 months later. A histopathological examination was performed, and the lingual mass was diagnosed as squamous cell carcinoma.