[KSCI] Korea Science Citation Index Service

http://dx.doi.org/10.4014/jmb.0800.189

Surfactin Blocks NO Production in Lipopolysaccharide-activated Macrophages by Inhibiting $NF-{\kappa}B$ Activation

Byeon, Se-Eun (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University)
Lee, Yong-Gyu (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University)
Kim, Byung-Hun (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University)
Shen, Ting (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University)
Lee, Sang-Yeol (Department of Life Science, Kyungwon University)
Park, Hwa-Jin (Department of Biomedical Laboratory Science, College of Biomedical Science and Engineering, Regional Research Center, Inje University)
Park, Seung-Chun (College of Veterinary Medicine, Kyungpook National University)
Rhee, Man-Hee (College of Veterinary Medicine, Kyungpook National University)
Cho, Jae-Youl (School of Bioscience and Biotechnology, and Institute of Bioscience and Biotechnology, Kangwon National University)

Publication Information

Journal of Microbiology and Biotechnology / v.18, no.12, 2008 , pp. 1984-1989 More about this Journal

Abstract

Surfactin is a natural biosurfactant derived from Bacillus subtilis and has various biological activities such as anticancer, antiplatelet, and anti-inflammatory effects. In this study, the inhibitory mechanism of surfactin in NO production from macrophages was examined. Surfactin down regulated LPS-induced NO production in RAW264.7 cells and primary macrophages with $IC_{50}$ values of 31.6 and $22.4{\mu}M$ , respectively. Immunoblotting analysis showed that surfactin strongly blocked the phosphorylation of IKK and $l{\kappa}B{\alpha}$ and the nuclear translocation of $NF-{\kappa}B$ (p65). Therefore, these data suggest that surfactin may act as a bacterium-derived anti-inflammatory agent with anti- $NF-{\kappa}B$ activity.

Keywords

Surfactin; Bacillus subtilis; nitric oxide; anti-inflammatory effect; lipopolysaccharide; $NF-{\kappa}B$ ;

Citations & Related Records

Times Cited By KSCI : 2 (Citation Analysis)
Times Cited By Web Of Science : 11 (Related Records In Web of Science)

Reference
Cited By KSCI

1	Cho, J. Y., A. R. Kim, H. G. Joo, B. H. Kim, M. H. Rhee, E. S. Yoo, D. R. Katz, B. M. Chain, and J. H. Jung. 2004. Cynaropicrin, a sesquiterpene lactone, as a new strong regulator of CD29 and CD98 functions. Biochem. Biophys. Res. Commun. 313: 954-961 DOI ScienceOn
2	Choi, E. K., H. C. Jang, J. H. Kim, H. J. Kim, H. C. Kang, Y. W. Paek, et al. 2006. Enhancement of cytokine-mediated NFkappaB activation by phosphatidylinositol 3-kinase inhibitors in monocytic cells. Int. Immunopharmacol. 6: 908-915 DOI ScienceOn
3	Denkers, E. Y., B. A. Butcher, L. Del Rio, and L. Kim. 2004. Manipulation of mitogen-activated protein kinase/nuclear factorkappaB- signaling cascades during intracellular Toxoplasma gondii infection. Immunol. Rev. 201: 191-205 DOI ScienceOn
4	Fishman, P., S. Bar-Yehuda, L. Madi, L. Rath-Wolfson, A. Ochaion, S. Cohen, and E. Baharav. 2006. The PI3K-NF-kappaB signal transduction pathway is involved in mediating the antiinflammatory effect of IB-MECA in adjuvant-induced arthritis. Arthritis Res. Ther. 8: R33 DOI
5	Hatziieremia, S., A. I. Gray, V. A. Ferro, A. Paul, and R. Plevin. 2006. The effects of cardamonin on lipopolysaccharide-induced inflammatory protein production and MAP kinase and NFkappaB signalling pathways in monocytes/macrophages. Br. J. Pharmacol. 149: 188-198
6	Kim, S. Y., J. Y. Kim, S. H. Kim, H. J. Bae, H. Yi, S. H. Yoon, et al. 2007. Surfactin from Bacillus subtilis displays antiproliferative effect via apoptosis induction, cell cycle arrest and survival signaling suppression. FEBS Lett. 581: 865-871 DOI ScienceOn
7	Malyshev, I. Y. and A. Shnyra. 2003. Controlled modulation of inflammatory, stress and apoptotic responses in macrophages. Curr. Drug Targets Immune Endocr. Metabol. Disord. 3: 1-22
8	Yoshimura, A. 2006. Signal transduction of inflammatory cytokines and tumor development. Cancer Sci. 97: 439-447 DOI ScienceOn
9	Cho, J. Y., K. U. Baik, J. H. Jung, and M. H. Park. 2000. In vitro anti-inflammatory effects of cynaropicrin, a sesquiterpene lactone, from Saussurea lappa. Eur. J. Pharmacol. 398: 399-407 DOI ScienceOn
10	Cho, J. Y., J. Park, P. S. Kim, E. S. Yoo, K. U. Baik, and M. H. Park. 2001. Savinin, a lignan from Pterocarpus santalinus inhibits tumor necrosis factor-alpha production and T cell proliferation. Biol. Pharm. Bull. 24: 167-171 DOI ScienceOn
11	Kim, H. G., B. Shrestha, S. Y. Lim, D. H. Yoon, W. C. Chang, D. J. Shin, et al. 2006. Cordycepin inhibits lipopolysaccharideinduced inflammation by the suppression of NF-kappaB through Akt and p38 inhibition in RAW 264.7 macrophage cells. Eur. J. Pharmacol. 545: 192-199 DOI ScienceOn
12	Hardy, K. and N. H. Hunt. 2004. Effects of a redox-active agent on lymphocyte activation and early gene expression patterns. Free Radic. Biol. Med. 37: 1550-1563 DOI ScienceOn
13	Beitz, L. O., D. A. Fruman, T. Kurosaki, L. C. Cantley, and A. M. Scharenberg. 1999. SYK is upstream of phosphoinositide 3- kinase in B cell receptor signaling. J. Biol. Chem. 274: 32662-32666 DOI
14	Hwang, M. H., Z. Q. Chang, E. H. Kang, J. H. Lim, H. I. Yun, M. H. Rhee, K. S. Jeong, and S. C. Park. 2008. Surfactin C inhibits Mycoplasma hyopneumoniae-induced transcription of proinflammatory cytokines and nitric oxide production in murine RAW 264.7 cells. Biotechnol. Lett. 30: 229-233 DOI ScienceOn
15	Lim, J. H., B. K. Park, M. S. Kim, M. H. Hwang, M. H. Rhee, S. C. Park, and H. I. Yun. 2005. The anti-thrombotic activity of surfactins. J. Vet. Sci. 6: 353-355
16	Hwang, M. H., J. H. Lim, H. I. Yun, M. H. Rhee, J. Y. Cho, W. H. Hsu, and S. C. Park. 2005. Surfactin C inhibits the lipopolysaccharide-induced transcription of interleukin-1beta and inducible nitric oxide synthase and nitric oxide production in murine RAW 264.7 cells. Biotechnol. Lett. 27: 1605-1608 DOI ScienceOn
17	Jung, K. K., H. S. Lee, J. Y. Cho, W. C. Shin, M. H. Rhee, T. G. Kim, et al. 2006. Inhibitory effect of curcumin on nitric oxide production from lipopolysaccharide-activated primary microglia. Life Sci. 79: 2022-2031 DOI ScienceOn
18	Carrillo, C., J. A. Teruel, F. J. Aranda, and A. Ortiz. 2003. Molecular mechanism of membrane permeabilization by the peptide antibiotic surfactin. Biochim. Biophys. Acta 1611: 91-97 DOI ScienceOn
19	Lee, J. Y., J. Y. Kim, Y. G. Lee, W. C. Shin, T. Chun, M. H. Rhee, and J. Y. Cho. 2007. Hydroquinone, a reactive metabolite of benzene, reduces macrophage-mediated immune responses. Mol. Cells 23: 198-206

7	(2008) International immunopharmacology Surfactin inhibits immunostimulatory function of macrophages through blocking NK-κB, MAPK and Akt pathway / 9 (7) , 886
13	(2008) The Journal of biological chemistry Akt Cys-310-targeted Inhibition by Hydroxylated Benzene Derivatives Is Tightly Linked to Their Immunosuppressive Effects / 285 (13) , 9932
2	(2008) Applied microbiology and biotechnology Microbial biosurfactants production, applications and future potential / 87 (2) , 427
11	Ho-Sik Rho. (2010) Bulletin of the Korean Chemical Society Inhibitory Activities of Kojyl Thioether Derivatives against Nitric Oxide Production Induced by Lipopolysaccharide / 31 (11) , 3463
8	Yongwoo Jung. (2008) Acta pharmacologica Sinica 8-(Tosylamino)quinoline inhibits macrophage-mediated inflammation by suppressing NF-κB signaling / 33 (8) , 1037
1	(2008) Inflammation A Leaf Methanolic Extract of Wercklea insignis Attenuates the Lipopolysaccharide-Induced Inflammatory Response by Blocking the NF-κB Signaling Pathway in RAW 264.7 Macrophages / 35 (1) , 321
1	Woo Seok Yang. (2012) Journal of ethnopharmacology Src/NF-κB-targeted inhibition of LPS-induced macrophage activation and dextran sodium sulphate-induced colitis by <I>Archidendron clypearia</I> methanol extract / 142 (1) , 287
3	Yanyan Yang. (2008) Journal of ethnopharmacology Methanol extract of Osbeckia stellata suppresses lipopolysaccharide- and HCl/ethanol-induced inflammatory responses by inhibiting Src/Syk and IRAK1 / 143 (3) , 876
None	Van Thai Ha. (2008) Mediators of inflammation NF- <I>κ</I> B/AP-1-Targeted Inhibition of Macrophage-Mediated Inflammatory Responses by Depigmenting Compound AP736 Derived from Natural 1,3-Diphenylpropane Skeleton / 2014 (None) , 354843
None	Shi Hyoung Kim. (2008) Mediators of inflammation The Dietary Flavonoid Kaempferol Mediates Anti-Inflammatory Responses via the Src, Syk, IRAK1, and IRAK4 Molecular Targets / 2015 (None) , 904142
None	Jeong-Oog Lee. (2015) Mediators of inflammation ATP-Binding Pocket-Targeted Suppression of Src and Syk by Luteolin Contributes to Its Anti-Inflammatory Action / 2015 (None) , 967053
5	Jun Ho Kim. (2008) Biomolecules & therapeutics Fisetin Suppresses Macrophage-Mediated Inflammatory Responses by Blockade of Src and Syk / 23 (5) , 414
5	Nak Yoon Sung. (2015) The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology Scutellarein Reduces Inflammatory Responses by Inhibiting Src Kinase Activity / 19 (5) , 441
1	Yong Kim. (2016) The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology (E)-3-(3-methoxyphenyl)-1-(2-pyrrolyl)-2-propenone displays suppression of inflammatory responses via inhibition of Src, Syk, and NF-κB / 20 (1) , 91
15	(2008) Applied microbiology and biotechnology Biological activity of lipopeptides from Bacillus / 101 (15) , 5951
None	(2008) Frontiers in pharmacology Anticancer Activities of Surfactin and Potential Application of Nanotechnology Assisted Surfactin Delivery / 8 (None) , 761
2	Tao Yu. (2008) Journal of ginseng research Ginsenoside Rc from Panax ginseng exerts anti-inflammatory activity by targeting TANK-binding kinase 1/interferon regulatory factor-3 and p38/ATF-2 / 41 (2) , 127
4	Sulgi Yoo. (2017) The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology Beauvericin, a cyclic peptide, inhibits inflammatory responses in macrophages by inhibiting the NF-κB pathway / 21 (4) , 449
2	(2008) The American journal of Chinese medicine <I>Momordica charantia</I> Inhibits Inflammatory Responses in Murine Macrophages via Suppression of TAK1 / 46 (2) , 435
1	(2008) Journal of animal science and biotechnology Effects of dietary Bacillus amyloliquefaciens supplementation on growth performance, intestinal morphology, inflammatory response, and microbiota of intra-uterine growth retarded weanling piglets / 9 (1) , 22
None	(2008) Frontiers in microbiology Biosurfactants: A Covid-19 Perspective / 11 (None) , 1341
2	(2008) Biomolecules Regulation of 8-Hydroxydaidzein in IRF3-Mediated Gene Expression in LPS-Stimulated Murine Macrophages / 10 (2) , 238
5	(2008) Cancers Molecular Signatures of <I>JMJD10/</I> MINA53 in Gastric Cancer / 12 (5) , 1141
11	(2020) Current drug metabolism Biosurfactants as a Novel Additive in Pharmaceutical Formulations: Current Trends and Future Implications / 21 (11) , 885

KSCI

Surfactin Blocks NO Production in Lipopolysaccharide-activated Macrophages by Inhibiting Activation

Surfactin Blocks NO Production in Lipopolysaccharide-activated Macrophages by Inhibiting $NF-{\kappa}B$ Activation