• 한국환경성돌연변이발암원학회지
• /
• 제10권2호
• /
• pp.85-92
• /
• 1990

동시화시킨 CHO세포를 재료로하여 EMS 혹은 BLM에 의해 유발된 염색체 이상에 미치는 APC, ddTTP, 그리고 NOV의 효과를 조사하였다. 세포의 동시화는 thymidine block방법을 사용하였다. APC, ddTTP, 그리고 NOV의 단독처리는 염색체 이상을 유발하지 않았다. EMS에 의해 유발된 염색체 이상은 late G$_1$과 early S기에 높은 감수성을 나타내었고, BLM에 의해 유발된 염색체 이상은 G$_2$기에 가장 높은 감수성을 나타내었다. APC를 후처리할 경우 late G$_1$기와 early S기에서 EMS에 의해 유발된 염색체이상을 증가시켰다. 그러나 ddTTP나 NOV를 복합처리하기전 NOV를 전처리하면 late G$_1$기와 early S기에서의 염색체 이상이 증가되었다. 이런 결과들은 여러 가지 돌연변이원에 따라 각기 다른 세포내 반응이 유발되며 염색체 이상에서의 각 효소들의 활성 또한 다른 것으로 추측된다.

• 한국환경성돌연변이발암원학회지
• /
• 제21권1호
• /
• pp.14-22
• /
• 2001

The detection of many synthetic chemicals used in industry that may pose a genetic hazard in our environment is of great concern at present. In this respect, administrative authorities has great concern to regulate and to evaluate the chemical hazard to environment and human health. The clastogenicity of 28 synthetic chemicals was evaluated in Chinese hamster lung fibroblast cells in vitro. Glycidylacrylate which is one of the most cytotoxic chemical among 28 chemicals tested revealed clastogenicity in the range of 0.31-1.25 $\mu\textrm{g}$/$m\ell$ both in the presence and absence of metabolic activation system. Neopentyl glycol (340-1360 $\mu\textrm{g}$/$m\ell$) also revealed weak positive result both in the presence and absence of metabolic activation system. Cyanoguanidine (/$420.5-841 $\mu\textrm{g}$m\ell$) and N-butylchloride ($231.5-926 $\mu\textrm{g}$/m\ell$) revealed weak positive result only in the absence of S-9 metabolic activation system. Nevertheless total aberration percentages of N-butylchloride in the presence of metabolic activation system, and 3,4＇-dichlorobenztrifluoride in the absence of S-9 metabolic activation revealed above 5% aberration, there is no statistical significance. From the results of chromosomal aberration assay with 28 synthetic chemicals in Chinese hamster lung cells, glycidylacrylate (CAS No. 106-90-0), neopentyl glycol (CAS No. 126-30-7), N-butyl chloride (CAS No. 109-69-3) and cyanoguanidine (CAS No. 461-58-5) revealed positive clastogenic results in this study.

• 한국환경성돌연변이발암원학회지
• /
• 제22권3호
• /
• pp.149-156
• /
• 2002

The detection of many synthetic chemicals used in industry that may pose a genetic hazard in our environment is of great concern at present. Since these substances are not limited to the original products, and enter the environment, they have become widespread environmental pollutants, thus leading to a variety of chemicals that possibly threaten the public health. In this respect, to regulate and to evaluate the chemical hazard will be important to environment and human health. The clastogenicity of 18 synthetic chemicals was evaluated in Chinese hamster lung fibroblast cells in vitro. 4-Chloro-3,5-dimethyl phenol (CAS No. 88-04-0) induced chromosomal aberrations with significance at the concentration of 15.7 $\mu\textrm{g}$/$m\ell$ both in the presence and absence of metabolic activation system. Phenoxybenzene (CAS No. 101-84-8) which is one of the most cytotoxic chemical among 18 chemicals tested revealed no clastogenicity in the range of 0.11-0.43 $\mu\textrm{g}$/$m\ell$ both in the presence and absence of metabolic activation system. From the results of chromosomal aberration assay with 18 synthetic chemicals in Chinese hamster lung cells in vitro, 4-chloro-3,5-dimethyl phenol (CAS No. 88-04-0) revealed weak positive clastogenic results in this study.

• Toxicological Research
• /
• 제22권2호
• /
• pp.145-151
• /
• 2006

We have investigated the genotoxicity of STB-HO-BM using in vitro and in vivo system such as Ames reverse mutation test, chromosomal aberration test and micronucleus test. in Ames reverse mutation test, STB-HO-BM treatment at the dose range up to 5,000 ug/plate did not induce mutagenicity in Salmonella typhimurium TA98, TA100, TA102, TA1535, TA 1537 and in Escherichia coli WP2 uvrA with and without metabolic activation. Any significant aberration wasn't observed in chinese hamster lung (CHL) fibroblast cells treated with STB-HO-BM at the concentration of 12.5, 2.5, 5 mg/ml both in the absense and presence of metabolic activation system. In mouse micrnucleus test, no significant increase in the occurrence of micronucleated polychromatic erythrocytes was observed in ICR male mice orally administered with STB-HO-BM at the doses of 0.5, 1.0, 2.0 g/kg. These results indicate that STB-HO-BM has no mutagenic potential under the condition in this study.

• Molecular & Cellular Toxicology
• /
• 제2권2호
• /
• pp.89-96
• /
• 2006

The detection of many synthetic chemicals used in industry that may pose a genetic hazard in our environment is of great concern at present. Since these substances are not limited to the original products, and enter the environment, they have become widespread environmental pollutants, thus leading to a variety of chemicals that possibly threaten the public health. In this respect, to regulate and to evaluate the chemical hazard will be important to environment and human health. The clastogenicity of 11 synthetic chemicals was evaluated in Chinese hamster lung fibroblast cells in vitro. 1-Chloro-3-bromopropane CAS No. 109-70-6) induced chromosomal aberrations with significance at the concentration of $185.0\;{\mu}g/mL\;and\;1,600\;{\mu}g/mL$ both in the presence and absence of metabolic activation system, respectively. Triphenyl phosphite (CAS No. 101-02-0), which is one of the most cytotoxic chemical among 11 chemicals tested revealed no clastogenicity in the range of $95.0-4.9\;{\mu}g/mL$ both in the presence and absence of metabolic activation system. From the results of chromosomal aberration assay with 11 synthetic chemicals in Chinese hamster lung cells in vitro, 1-chloro-3-bromopropane revealed a positive clastogenic result in this study.

• Environmental Analysis Health and Toxicology
• /
• 제15권4호
• /
• pp.157-163
• /
• 2000

2- [(4- Cyanophenyl)amino] -3-chloro-1, 4- naphthalenedione (NQ-Y15) was asssayed for its genotoxic potential by using Salmonella typhimurium reversion assay and in vitro chromosome aberration test on Chinese hamster lung cells. In the Ames test, NQ-Y15 induced his + revertants of Salmonella typhimurium TA 98 and TA1537, reaching levels twice the negative control values. But, NQ-Y15 induced only his+ revertants of Salmonella typhimurium TA1537 more than twice the control values under the condition with metabolic activation system. In the cytogenetic test on chinese hamster lung cells. NQ -Y15 showed significant chromosomal aberrations, but the incidence was significantly reduced in the presence of metabolic activation.

• Journal of Microbiology and Biotechnology
• /
• 제16권5호
• /
• pp.817-820
• /
• 2006

The potential genotoxicity of methylsulfonylmethane, a crystalline organic sulfur, derived from chemically modified lignin from plants was evaluated using in vitro and in vivo assays. In the bacterial reverse mutation test using Salmonella typhimurium TA98, TA100, TA1535, and TA1538, methylsulfonylmethane did not induce any significant increase of His' revertants. In the in vitro chromosome aberration test using Chinese Hamster Lung (CHL) cells, no aberration effects were seen. In the in vivo evaluation using a micronucleus test, negative results were obtained. Accordingly, the results indicated that methylsulfonylmethane is not genotoxic and its use is unlikely to present a potential hazard.

• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1995년도 춘계학술대회
• /
• pp.123-123
• /
• 1995

To evaluate the genetic toxicity of NP-77A which is selected as the candidate of anti-HBV agent, we performed ames test, micronucleus test, and chromosome aberration test on the CHL cell in vitro. The Ames test was carried out with 5 fold diluted 5 concentrations from 25mg/plate using S. typhimurium and E.coli. After 48hrs incubation, revertant colony numbers was calculated with and without metabolic activation system. In vivo micronucleus test, we investigated the rate of the occurrence of micronucleus after I.P. administration to mice. Andalso, we observed the incidence rate of cells with chromosomal aberration by NP-77A treatment using CHL cell line.

• Environmental Analysis Health and Toxicology
• /
• 제18권2호
• /
• pp.121-129
• /
• 2003

Pendimethalin [N-(1-ethyl-propyl)-2, 6-dinitro-3, 4-xylidine, $C_{13}$H$_{19}$N$_3$O$_4$, M.W. = 281.3, CAS No. 40481-42-1]는 제초제의 일종으로, 본 연구에서는 박테리아 복귀 돌연변이 시험과 포유동물 세포를 이용한 염색체 이상 시험 및 마우스를 이용한 in vivo 소핵 시험을 수행하여 pendimethalin의 유전독성을 평가하였다. 박테리아 복귀 돌연변이 시험에서 pendimethalin은 Salmonella thphimurium TA98, TA1537 균주의 경우, 대사 활성계 존재와 부재시,TA100의 경우는 대사 활성계 부재시에만 313∼5,000 $\mu\textrm{g}$/p1a1e의 범위에서 농도의존적인 돌연변이율의 증가를 보여주었고, TA1535의 경우에는 대사 활성계 존재시 약간의 돌연변이가 증가되는 것을 관찰할 수 있었다. 그러나 대사 활성계 부재시 TA1535와 대사 활성계 존재시 TA100균주의 경우에는 돌연변이 유발능을 관찰할 수 없었다. 한편 포유동물 세포인 Chinese hamster lung(CHL) fibroblast를 이용한 염색체 이상 시험에서 pendimethalin은 대사 활성계 존재 및 부재시 2.32∼9.28 $\mu\textrm{g}$/ml 농도에서 clastogenicity를 보이지 않았다. 또한 203∼810 mg/kg의 pendimethalin을 구강 투여한 마우스의 골수세포를 이용한 in vivo소핵 시험의 결과에서도 통계적으로 유의한 소핵 유발능을 관찰할 수 없었다.다.

• 한국식품위생안전성학회지
• /
• 제16권2호
• /
• pp.145-151
• /
• 2001

유아 식품중의 병마개 등에 약 30%까지 함유되어 합성수지 및 고무의 가소제로 많이 사용되고 있으나 최근 유럽등지에서 식품으로의 유리가 보고되어 안전성에 대한 재평가가 요구되어지고 있는 에폭시화 대두유 (Epoxidized soy bean oil, ESBO)의 유전독성을 평가하기 위해서 박테리아를 이용한 복귀돌연변이시험과 포유류 배양세포를 대상으로 검색하는 체외 염색체이상시험, 설치류의 조혈세포를 이용한 체내 소핵시험을 수행하였다. Salmonella typhimurium TA98, TA100, TA1535, TA1537, TA102균주를 이용한 복귀돌연변이 시험결과 직접법 및 대사활성화법에서 돌연변이 유발성을 가지지 않는 음성의 결과를 나타내었으며, chinese hamster lung cell을 이용한 염색체이상시험을 실시한 결과, 직접법 및 대사활성화법에서 염색체이상 유발작용을 보이지 않는 음성의 결과를 나타내었다. 또한 성별, 연령별에 따라서 마우스 골수세포를 이용한 소핵시험에서 에폭시화 대두유는 미성숙 적혈구중 유의성 있는 소핵 유발은 관찰되지 않았다. 이상의 결과를 종합하여, 본 시험조건 중 에폭시화 대두유는 in vitro 시험인 Salmonella 균주를 이용한 복귀돌연변이시험과 포유동물 배양세포를 이용한 염색체이상시험 및 in vivo 시험인 마우스를 이용한 소핵시험에서 유전독성을 유발하지 않는 것으로 판단된다.