• KOREAN JOURNAL OF CROP SCIENCE
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• v.53 no.2
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• pp.125-130
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• 2008

This study analyzed the growth, seed quality, and yield of major early-maturing soybean cultivars by comparing them in order to utilize the research results in the selection of early-maturing soybean cultivars in multi-cropping farms in the Southern area. This field trial was conducted at Naju region (latitude $35^{\circ}04'N$, longitude $126^{\circ}54'E$), Jeonnam, with planting on June 15. The maturing date for Keunol-kong and Hwaseong-put-kong was found to be around September 12, which was earlier than other cultivars. Thus, there were advantages to introducing a cropping system as well as having good seed quality and high yield. On the other hand, the maturing date for Saeol-kong and Sinrok-kong was found to be around September 20, which was a little bit late; however, the seed quality of the cultivars was good and they had a high yield. Therefore, if we want to sow the following crops of soybeans around mid-September, Keunol-kong and Hwaseong-put-kong are advantageous, while for the seeding around late September, Saeol-kong and Sinrok-kong would be good. This study was also performed to identify the limitation time for early harvesting by reviewing seed quality and yield of major early-maturing soybean cultivars according to early harvesting. When harvesting Keunol-kong on September 6, which was six days earlier than the optimal harvesting time (September 12), there was no difference in seed weight, yield, or seed quality than those of the harvested at the optimum maturing time. As for Saeol-kong, when harvesting on September 18, which was six days earlier than the optimal harvesting time (September 24), there was no difference in seed weight, yield, or seed quality than those of the harvested at the optimum maturing time. Therefore, the stable limitation time for early harvesting of Keunol-kong and Saeol-kong was concluded to be six days earlier than the optimal harvesting time.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.58 no.3
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• pp.239-252
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• 2013

Agronomic characteristics and grain properties of Korean wheat of 175 farmers in nationwide for two years, 2010/2011 and 2011/2012, were evaluated to support basic data for improving grain quality of Korean wheat and enhancing Korean wheat consumption. Agronomic characteristics, including culm length, spike length, number of $spike/m^2$ and rate of off-type plant, and grain properties, including 1000 kernel weight, test weight, moisture, ash and protein content, were influenced by year and location. Number of $spike/m^2$, test weight, moisture, ash and protein content of wheat cultivated in 2011 were higher than those of 2012 and culm length, spike length, rate of off-type plant and 1000 kernel weight of 2012 were higher than those of 2011. Wheat cultivated in southern part of Korea showed higher culm length and 1000 kernel weight and lower test weight than those of northern part of Korea. Spike length, number of $spike/m^2$ and test weight were reduced by additional fertilization after mid of March, although there was no significant difference between date of additional fertilization and grain properties. Cultivated wheats in Jeollabuk-do showed lower ash content and higher protein content than those of other provinces and cultivated wheats in Jeollanam-do exhibited higher ash content than that of other provinces. As amount of fertilization increased, culm length, 1000 kernel weight and protein content increased and spike length and ash content were decreased, although date of additional fertilization did not effect on agronomic characteristics and grain properties. Amount of fertilization was positively correlated with 1000 kernel weight and protein content (r = 0.159, P < 0.05 and r = 0.212, P < 0.01, respectively) and was negatively correlated with ash content (r = -0.185, P < 0.05). Thousand kernel weight was negatively correlated with ash content (r = -0.226, P < 0.01) and positively correlated with protein content (r = 0.207, P < 0.01). Ash content increased as test weight and culm length decreased and 1000 kernel weight was influenced by culm and spike length (r = 0.397, P < 0.001 and r = -0.205, P < 0.01, respectively).

• Journal of Dairy Science and Biotechnology
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• v.36 no.2
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• pp.106-120
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• 2018

Since 2018, the production and sales of ram-milk cheese ripened for over 60 days has been permitted in South Korea. Hence, this study aimed to examine the microbiological changes in 7 different types of Gouda cheese. During the aging period, traditional raw-milk Gouda Cheeses 1 and 2 did not contain Salmonella spp. during the 60-day storage period and no E. coli after 20-day storage. Coliform bacteria were not detected in Cheese 1 after 40 days; however, they were detected in Cheese 2 up to 60 days. Salmonella spp. were inhibited during the 60-day storage period in Cheese 3 (Salmonella spp.-contaminated raw-milk Gouda cheese), Cheese 4 (Cheese 3 contaminated with lactic acid bacteria DH 5 isolated from Kefir) and Cheese 5 (Cheese 3 contaminated with lactic acid bacteria DN1 isolated from Kefir). In particular, inhibition of Salmonella spp. was more prominent in Cheese 4 and Cheese 5 than in Cheese 3. During 60-day storage, Cheese 6 had a significantly reduced lactic acid bacteria. Furthermore, in Cheese 7, E. coli, E. Salmonella ssp. were rarely detected, and lactic acid bacteria were slightly greater in Cheese 7 than in other cheeses during the 60-day period. Moreover, all samples from Cheese 1 to Cheese 7 were not contaminated with Listeria monocytogenes, Staphylococcus aureus, Clostridium perfringens, and E. coli O157:H7.

• Journal of Dairy Science and Biotechnology
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• v.30 no.1
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• pp.55-67
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• 2012

We compared the general composition of Mozzarella cheese manufactured by the traditional method and by an ultrafiltration technique. The comparison of the general components between raw milk and the retentate of ultrafiltration (CF 2 and CF 3) demonstrated the following. The fat, protein, and non-fat solid contents of the retentate were higher than those of raw milk, but the lactose content was lower. The effect of 3 factors-fat content (0.5% and 3%), bacterial populations (30,000 CFU/mL and 100,000 CFU/mL), and ratio of added starter and rennet (50, 65, or 80)-on Mozzarella cheese made by the traditional method and made using the retentate of ultrafiltration (CF 2 and CF 3) were compared. Total solids (TS), fat, lactose, total nitrogen (TN), water soluble nitrogen (WSN), non-protein nitrogen (NPN), and salt (NaCl) were affected by fat content, bacterial population, and the ratio of starter and rennet added, but the ash content was not affected. During storage at $4^{\circ}C$ over a period of 3 months, the TS, WSN, NPN, and NaCl contents increased significantly (p<0.05), but fat, lactose, and TN contents decreased significantly (p<0.05), while ash contents remained constant. In whey, the protein, lactose, and solids not fat contents were higher in cheese made from ultrafiltration retentate than in traditional Mozzarella cheese. These results indicate that ultrafiltration can be applied to producing several Mozzarella-type cheeses in order to meet consumer needs.

• Journal of Dairy Science and Biotechnology
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• v.30 no.1
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• pp.1-10
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• 2012

The objective of this study was to evaluate the rheology and sensory properties of Mozzarella cheese manufactured by the traditional method and by an ultrafiltration technique and to attempt to develop a new type of Mozzarella cheese. We noted 3 observations. (1) The initial pH observed for raw milk and retentate of ultrafiltration (CF 2 and CF 3) did not vary much. However, there was a significant difference in the pH at stretching. The initial TAwas significantly different between the raw milk and retentate obtained by ultrafiltration (CF 2 and CF 3). However, no differences were observed in TA at stretching. (2) The predicted yield (Y50), adjusted to 50% total solids (TS), was affected by the concentration factor, fat content, and addition of starter and rennet. The Y50 increased with the concentration factor. 3) Hardness, elasticity, cohesiveness, and brittleness were affected by fat content, and addition of starter and rennet. The hardness, elasticity, and brittleness of Mozzarella cheese made from the retentate of ultrafiltration (CF 2 and CF 3) were higher than in Mozarella cheese made by the traditional method; however, the cohesiveness decreased during storage at $4^{\circ}C$ over a period of 3 months. (4) Flavor, body texture, appearance, color, and firmness decreased with bacterial count, fat content, and addition of starter and rennet. The results of the sensory evaluation of the Mozzarella cheese made from the retentate of ultrafiltration (CF 2 and CF 3) changed during storage at $4^{\circ}C$ over a 3-month period.

• Horticultural Science & Technology
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• v.30 no.4
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• pp.357-362
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• 2012

Proper light intensity, shading tolerance, potting media, and fertilization level were investigated to develop Orostachys iwarenge for. magnus (Korean name, Ullungyeonwhabawisol) as a potted ornamental plant. The plants were grown under different light intensity (52, 82, 90, and 97% shading). The best growth was shown at 52% shading, which indicated that the proper light intensity for O. iwarenge for. magnus is less than 52% of shading. Plant growth decreased severely at 82% shading and leaf color became lighter as the shading rate increased, which indicated that O. iwarenge for. magnus has no tolerance against low light intensity. To select a proper potting media, decomposed granite (DG), fertilizer-amended media (FAM), river sand (RS) were used as potting medium with different ratio of 60:20:20 (DG:FAM:RS, v/v/v), 80:20 (DG:FAM, v/v), 60:40 (DG:FAM, v/v), and 20:80 (FAM:RS, v/v). DG:FAM:RS (60:20:20) showed the highest values in shoot fresh weight, plant width, and number of runner in potted O. iwarenge for. magnus. Fresh weight of shoot part was 16 g in DG:FAM:RS (60:20:20), which was about 2 folds of those at the other medium. At the experiment for selection of proper fertilization level, plants showed a better growth as the concentration of hyponex solution and application frequency increased. Once drenching 1 week interval of hyponex solution diluted by 1,000 folds brought the highest results in fresh weight, plant width, and runner number. Particularly, fresh weight of shoot part was 35 g at once drenching per week of 1,000 folds solution, indicating 84% improvement comparing with non treatment (19 g).

• Horticultural Science & Technology
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• v.28 no.5
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• pp.743-749
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• 2010

Objective of this research was to investigate the effect of various potassium concentrations in fertilizer solution on growth of and nutrient uptake by 'Chugyang' eggplant ($Solanum$ $melongena$ L.). Tissue analyses were conducted to determine the threshold levels of potassium in plants when disorders develop for potassium. The lower leaves of K deficient plants became spotted, yellowing in color, and finally necrosis occurred. The necrosis spread inward and upward. K toxic plants developed necrotic spot at the margin of old leaves and the surface of old leaves were twisted. The tissue K contents in the most recently fully expanded leaves and dry weight of full above ground plant tissue at 35 days after transplanting showed linear and quadratic response to elevated K concentrations with the equation of y=1.127+0.3147x ($R^2$=0.8916) and y=14.92+2.2743x-$0.1402x_2$ ($R^2$=0.8659). When 10% reduction in dry weight set to threshold levels, optimum tissue K contents are in the range from 2.1 to 5.1%. The yield through 150 days after transplanting and tissue K contents at 150 days after transplanting also showed quadratic and linear responses to elevated potassium concentrations in fertigation solution with the equation of y=153.24+345.5x-$18.46x^2$ ($R^2$=0.8620) and y=0.9921+0.3860x ($R^2$=0.9611), respectively. When the 10% reduction in yield is set to threshold levels, the tissue K contents for maximum yield should be around 3.4% to 5.9% in most recently fully expanded leaves.

• Journal of Dairy Science and Biotechnology
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• v.34 no.4
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• pp.203-216
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• 2016

Probiotic microorganisms are thought to provide health benefits when consumed. In 2001, the World Health Organization defined probiotics as "live microorganisms which confer a health benefit on the host, when administered in adequate amounts." Three methods for screening potential probiotics have currently widely available. (1) In vitro assays of potential probiotics are preferred because of their simplicity and low cost. (2) The use of in vivo approaches for exploring various potential probiotics reflects the enormous diversity in biological models with various complex mechanisms. (3) Potential probiotics have been analyzed using several genetic and omics technologies to identify gene expression or protein production patterns under various conditions. However, there is no ideal procedure for selecting potential probiotics than testing cadidate strains on the target population. Hence, in this review, we provide an overview of the different methodologies used to identify new probiotic strains. Furthermore, we describe futre perspectives for the use of in vitro, in vivo and omics in probiotic research.

• Herbal Formula Science
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• v.19 no.1
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• pp.51-58
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• 2011

Objectives : This study was performed to investigate applications of 171 prescriptions including Astragali Radix and Angelicae gigantis Radix in Dongeuibogam. Methods : 171 prescriptions including Astragali Radix and Angelicae gigantis Radix which have been used separately or concurrently in Oriental Medicine for a long time as a treatment for various disease in Dongeuibogam were studied through order of frequency, symptoms, dosages, etc. Results : 1. 27 times(15.79%) prescriptions are recorded in intumescence chapter, 21 times(12.28%) in fatigue chapter, 11 times(6.43%) in women chapter and 9 times(5.26%) in anus chapter, which are arranged in order of frequency. 2. Yin-yang deficiency treat herbs are ranked top, eliminating intumescence methods, bloody stool, discharging blood from one's vagina, yang deficiency treat herbs are ranked in order of frequency among 107 symptoms in prescriptions including Astragali Radix and Angelicae gigantis Radix. 3. The dosages of Astragali Radix which is more used than Angelicae gigantis Radix are ranged from 3 puns:1 pun ~ 2 nyangs:1.5 nyangs. 1 jeon:5 puns is recorded 8 times(14.29%), 2 jeon:1 jeon is 7 times(12.50%), 1 jeon:3 puns and 1 jeon:7 puns are 24 times(11.2%), 1 nyang is 21 times(9.8%), same amount is 11 times(5.1%), 5 pun is 4 times(7.14%) each, 1.5 jeon:5 puns is 3 times(5.36%) used among 56 prescriptions including Astragali Radix and Angelicae gigantis Radix. 4. The dosages of Astragali Radix which is used same amount Angelicae gigantis Radix are ranged from 2.5 puns each ~ 2 nyangs each, 2.5 chons, same amount each. 1 jeon each is recorded 36 times(38.71%), 1 nyang each is 15 times(16.13%), 0.7 jeon each is recorded 12 times(12.90%), 0.5 jeon each is recorded 6 times(6.45%) used among 93 prescriptions including Astragali Radix and Angelicae gigantis Radix. 5. The dosages of Astragali Radix which is less used than Angelicae gigantis Radix are ranged from 3 puns:7 pun ~ 5 nyangs:12 nyangs. 1 jeon:1.5 jeons is recorded 3 times(13.64%), 0.8 jeon:1 jeon and 1 nyang:2 nyangs are 2 times(9.09%) each, the others are 1 time(4.55%) used among 22 prescriptions including Astragali Radix and Angelicae gigantis Radix. 6. The dosages of (Astragali Radix $\gg$ Angelicae gigantis Radix), (Astragali Radix = Angelicae gigantis Radix) and (Astragali Radix $\ll$ Angelicae gigantis Radix) which are recorded by counts (decoction groups: the other groups) are 52:4(92.86%:7.14%), 70:23(75.27%:24.73%), 13:9 (59.09%:40.91%) respectively. 7. The less using dosage of Astragali Radix and Angelicae gigantis Radix has a more percentage of decoctions groups, The more using dosage of Astragali Radix and Angelicae gigantis Radix has a more percentage of the other groups except decoctions. The less using prescriptions have an effects of controling yin and yang, enhancing blood and chi, etc, as a whole. The more using prescriptions as a form of compound powder have an effects in incurableness disease, chronic diseases, etc. Conclusions : The 171 prescriptions including Astragali Radix and Angelicae gigantis Radix in Dongeuibogam are mainly composed of Dangguibohyul-tang, Samool-tang, Sagoonja-tang and Gobangpoong-tang, Gamri-hwan, etc.

• Tuberculosis and Respiratory Diseases
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• v.59 no.4
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• pp.397-405
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• 2005

Background : Laminin-1 is known to have regular functions in the development and course of differentiation of the lungs. The morphogenesis and distribution of laminin-1 still remains as a mystery and its distribution and changes in the molecular structure of laminin-1 in the pathogenesis of the lung still is a subject of great controversy. In this study, experiments were done to delineate the distribution and changes in the amount of laminin-1 after inducing inflammation of the lungs by exposing experimental animals to CS gas and especially, to find compositions of laminin-1 within type II pneumocytes. Materials and Methods : The experimental subjects of study were newborn rats and the extracted tissue from the experimental rats were viewed under light microscope and electron microscope after the sections were treated with immunohistochemical methods and immunogold reaction methods using bounded gold particles. Results : 1) Lymphocytes and mononuclear phagocytes invaded the alveolar septa in the 2 day group rats after CS gas exposure and intense interstitial inflammation was seen in the 3 day group. 2) Laminin immunoreactions decreased to a moderate degree in the 2 and 3 day group rats after CS gas exposure and strong laminin immunoreactions were seen again in the 5 and 7 day group rats. 3) Gold particles in basal lamina of the lung blood-air barrier decreased and in the type I pneumocytes decreased in the 2 and 3 day group rats after CS gas exposure. 4) Gold particles were seen only on the surface of the cell membranes of type II pneumocytes of the 1 and 2 day group rats after CS gas exposure. 5) Few gold particles around the lamellar bodies and cytoplasm of type II pneumocytes in the control rat group and at 12 hours after CS gas exposure. Gold particles are seen only on the surface of type II pneumocytes of the 1 and 2 day group rats after CS gas exposure and are evenly distributed in small amounts in the cells of the 3 day group after CS gas exposure. Conclusion : CS gas exposure in the rats caused inflammation of lung alveolar septa and also induced a decrease in laminin-1 in basal lamina and loss of laminin-1 in the cytoplasm of type II pneumonocytes. As the inflammatory cells disappeared, an increase in the distribution of laminin-1 occurred. This reflects tissue regeneration functions of laminin-1 in the pneumocytes of rats and the distribution of laminin-1 in type II pneumocytes can be seen through the electron microscope using immunogold methods.