• Asian-Australasian Journal of Animal Sciences
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• 제29권12호
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• pp.1742-1747
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• 2016

A $2{\times}5$ factorial experiment, using 2 dietary methionine levels (0.28% and 0.48%) and 5 dietary choline levels (0, 394, 823, 1,239, and 1,743 mg/kg), was conducted to study the effects of dietary methionine status on choline requirements of starter white Pekin ducks from 7 to 28 days of age. Four hundred eighty 7-d-old male White Pekin ducks were randomly allotted to ten dietary treatments, each containing 6 replicate pens with 8 birds per pen. At 28 d of age, weight gain, feed intake, and feed/gain were measured and the legs of all ducks from each pen were examined for incidence of perosis. Perosis and growth depression were observed in choline-deficient ducks and supplementation of choline reduced perosis and significantly increased weight gain and feed intake regardless of dietary methionine levels (p<0.05). In addition, significant positive effects of dietary methionine supplementation on weight gain, feed intake, and feed/gain were observed at any choline level (p<0.05). Supplementation of 1,743 mg/kg choline in diets alleviated the depression of weight gain and feed intake caused by methionine deficiency at 0.28% methionine level. The interaction between choline and methionine influenced weight gain and feed intake of ducks (p<0.05). At 0.28% methionine level, 1,743 mg/kg choline group caused 4.92% and 3.23% amount of improvement in weight gain and feed intake compared with 1,239 mg/kg choline group, respectively. According to the broken-line regression, the choline requirements of starter Pekin ducks for weight gain and feed intake were 1,472 and 1,424 mg/kg at 0.28% methionine level and 946 and 907 mg/kg at 0.48% methionine level, respectively. It suggested the choline recommendations of starter Pekin ducks on a semi-purified diet were 1448 mg/kg at 0.28% methionine level and 927 mg/kg at 0.48% methionine level, respectively. Compared with the adequate methionine level, menthionine deficiency markedly increased the choline requirements of ducks.

• Journal of Nutrition and Health
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• 제37권1호
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• pp.61-67
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• 2004

This study was conducted to investigate the choline intake of Korean adults for the purpose of preparing a basal data required for the establishment of choline adequate intake (AI). The subjects of 56 Korean young adults were recruited from college students of 20 to 30 years old in Daejeon city. The aliquots of foods that the subjects ate for one day were collected with use of duplicate food collection method and choline content of one day meal directly was analyzed with the use of enzymatic method. Choline intakes of male subjects were in the range of 353.5∼1222.5 mg and those of female subjects were in the range of 213.1 ∼ 722.3 mg. Mean intakes of choline were 658.2 $\pm$ 243.9 mg/day in male subjects and 423.3 $\pm$ 133.6 mg/day in female, therefore choline intake of men was about 200mg higher than that of women. Median value in total subjects was 496 mg, male's median was 608.8 mg, female's median was 419.9 mg. When the subjects were devided into 4 groups by choline intake, as less than 75%, 75∼100%, 100∼125% and over 125% based on choline AI of USA (males: 550 mg, females: 425 mg), there was no significant difference between men (64.3%) and wemen (67.9%) in the distribution of the subjects whose choline intake is under the range of 75∼125% AI of USA. However, 10.7% of men and 21.4% of female had choline intake less than 75% AI of USA while the cases of choline intake higher than 125% AI were 25% in male and 10.7% in female. Thus, it is assumed that female case in choline-deficient state would be two times more than male. When adjusted by body weight, choline intake was 9.5 $\pm$ 3.4 mg/kg in men,8.1 $\pm$ 3.1 mg/kg in women and 8.8 $\pm$ 3,3 mg/kg in total subjects. And choline intake per 1,000 kcal of men, women and total subjects were 277.1 $\pm$ 78.4 mg, 275.9 $\pm$ 62.1 mg and 276.5 $\pm$ 70.1 mg respectively. From these results, it is suggested that these levels of 276.5 $\pm$ 70.1 mg/ 1,000 kcal or 8.8 $\pm$ 3.3 mg/kg B.W. can be used as a reference value for the establishment of AI of choline for Korean, because overall choline intake of these subjects was not in lower state compared to other nutrients intakes obtained from calculation of the food the subjects had taken.

• Biomolecules & Therapeutics
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• 제28권2호
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• pp.195-201
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• 2020

Prostate cancer is one of the most common cancers in men. Choline PET or PET/CT has been used to visualize prostate cancer, and high levels of choline accumulation have been observed in tumors. However, the uptake system for choline and the functional expression of choline transporters in prostate cancer are not completely understood. In this study, the molecular and functional aspects of choline uptake were investigated in the LNCaP prostate cancer cell line along with the correlations between choline uptake and cell viability in drug-treated cells. Choline transporter-like protein 1 (CTL1) and CTL2 mRNA were highly expressed in LNCaP cells. CTL1 and CTL2 were located in the plasma membrane and mitochondria, respectively. [³H]Choline uptake was mediated by a single Na⁺-independent, intermediate-affinity transport system in the LNCaP cells. The anticancer drugs, flutamide and bicalutamide, inhibited cell viability and [³H]choline uptake in a concentration-dependent manner. The correlations between the effects of these drugs on cell viability and [³H]choline uptake were significant. Caspase-3/7 activity was significantly increased by both flutamide and bicalutamide. Furthermore, these drugs decreased CTL1 expression in the prostate cancer cell line. These results suggest that CTL1 is functionally expressed in prostate cancer cells and are also involved in abnormal proliferation. Identification of this CTL1-mediated choline transport system in prostate cancer cells provides a potential new therapeutic target for the treatment of this disease.

• Asian-Australasian Journal of Animal Sciences
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• 제12권7호
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• pp.1090-1095
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• 1999

In order to minimize the occurrence of fatty liver, this study investigated how adding a high level of dietary choline or methionine affected the lipid metabolism of Tsaiya ducks. Feeding trials were conducted with sixty Tsaiya ducks during their growing period, when they were 8-12 weeks old, and during their laying period, 10-14 weeks after the onset of laying when they were 26-30 weeks old. The ducks were randomly assigned to one of three groups: a control group (basal diet), a group in which methionine was added to the basal diet, and a group in which choline was added to the basal diet. The levels of methionine and choline added were twice as high as the levels recommended by the NRC for layer-type chickens. Experimental results indicated that adding choline to the basal diet of growing ducks significantly (p<0.05) increased their body weight, while adding methionine significantly (p<0.05)reduced their body weight. Adding either choline or methionine reduced the ducks' liver fat content in both the growing and the laying periods (p<0.05). Ducks receiving added methionine or choline in their diets displayed enhanced egg production (p<0.05). Adding choline increased serum triacylglycerol (TG) in the laying period (p<0.05). Adding either choline or methionine did not significantly (p>0.05) affect the t-globulin level. Adding methionine increased the activity of liver malic dehydrogenase in both the growing and the laying periods, and increased fatty acid synthetase in the laying period (p<0.05). While adding choline markedly (p>0.05) increased VLDL and apo B in both the growing and the laying periods, it decreased HDL and apo A in the laying period (p<0.05). This study found that adding a high level of choline to the basal diets of Tsaiya ducks in both the growing and the laying periods had beneficial effects. Furthermore, adding methionine affected the lipid metabolism of Tsaiya ducks to a lesser extent than adding choline.

• Journal of Nutrition and Health
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• 제38권4호
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• pp.320-326
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• 2005

This study was conducted to estimate dietary choline intake of Koreans according to gender, age and region by using the data on per capita food intake in 1998 and 2001 and the data on choline contents of foods customarily eaten by Koreans. Sixty-four and 97 kinds of food items were used for the estimation of choline intake in 1998 and 2001, respectively. And these corresponded to approximately $90\%$ of the total amount of food intake and energy intake. Based on these food items and choline content, per capita choline intake of Koreans was estimated to be 623.0 mg and 602.4 mg in 1998 and 2001 respectively , 687.2 mg and 659.1 mg in 1998 and 2001 for male, 560.2 mg and 551.0 mg in 1998 and 2001 for female. While the number of foods considered in estimation was higher in 2001 than 1998, choline intake was lower in 2001. The estimated per capita choline intake of male population was higher than that of female population by 108-127 mg, possibly due to the difference in total amount of food intake. The age group with the highest choline intake was different between 2 time points. Although adolescents of 13- 19 years showed the highest intake (712.0 mg) in 1998, mid-aged adults of 30-49 years showed the highest choline intake (662.2 mg) followed by adolescents (645.7 mg) in 2001. In regional comparison, choline intake of the rural population (588.7 mg and 588.3 mg in 1998 and 2001 respectively) was lower than that of population in small to medium-size cities (625.1 mg and 603.9 mg) or in metropolitan cities (637.7 mg and 602.8 mg). This result revealed that per capita choline intake of the Korean population falls within the adequate range with some differences by age, sex and residing area.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
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• pp.106-106
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• 2003

Choline serves critical roles in the CNS both as a precursor of neurotransmitter and as an essential component of membrane phospholipids. The long-term maintenance of brain choline concentration is dependent on choline transport across the blood-brain barrier (BBB), And, we examined to elucidate the characteristics of transport of choline across the BBB using conditionally immortalized rat brain capillary endothelial cell line (TR-BBB) in vitro. The ［$^3$H］choline in TR - BBB was increased by time dependently, but independent on Na$\^$＋/, and the transport process is saturable with Michaelis-Menten constrant, Km of about 26 ${\mu}$M. The uptake of ［$^3$H］choline is susceptible for inhibition by various organic cationic compounds including hemicholinium-3, tetraethylammonium chloride (TEA) and $\ell$-carnitine. Also, we investigated the relationship of transport of choline and cationic drugs. The uptake of ［$^3$H］choline is inhibited by antioxidant, a-phenyl-n-tert-butyl nitrone (PBN) with IC$\sub$50/ of 1.2 mM. and by Alzheimer's disease therapeutics, such as acetyl $\ell$-carnitine, tacrine and donepezil. Also, choline uptake presented competitive inhibition with PBN, donepezil and acetyl $\ell$-carnitine in Lineweaver-Burk plot. In conclusion, TR-BBB cells express a saturable transport system for uptake of choline, and several cationic drugs may be transported into the brain by BBB choline transporter.

• Archives of Pharmacal Research
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• 제28권4호
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• pp.443-450
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• 2005

In the present study, we have characterized the choline transport system and examined the influence of various amine drugs on the choline transporter using a conditionally immortalized rat brain capillary endothelial cell line (TR-BBB) in vitro. The cell-to-medium (C/M) ratio of $[^3{H}]choline$ in TR-BBB cells increased time-dependently. The initial uptake rate of $[^3{H}]choline$ was concentration-dependent with a Michaelis-Menten value, $K_{m}$, of $26.2\pm2.7{\mu}M$. The $[^3{H}]choline$ uptake into TR-BBB was $Na^{+}-independent$, but was membrane potential-dependent. The $[^3{H}]choline$ uptake was susceptible to inhibition by hemicholinium-3, and tetraethy-lammonium (TEA), which are organic cation transporter substrates. Also, the uptake of $[^3{H}]choline$ was competitively inhibited with $K_{i}$ values of $274 {\mu}M, 251 {\mu}M and 180 {\mu}M$ in the presence of donepezil hydrochloride, tacrine and $\alpha-phenyl-n-tert-butyl nitrone$ (PBN), respectively. These characteristics of choline transport are consistent with those of the organic cation transporter (OCT). OCT2 mRNA was expressed in TR-BBB cells, while the expression of OCT3 or choline transporter (CHT) was not detected. Accordingly, these results suggest that OCT2 is a candidate for choline transport at the BBB and may influence the BBB permeability of amine drugs.

• 한국가금학회지
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• 제22권4호
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• pp.213-221
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• 1995

Five experiments were conducted to evaluate the effect of dietary supplemental folic acid(FA) in starting broiler chicks. In the first two experiments, basal diets based on corn and soybean meal contained 0.6 mg/kg FA but no supplemental methionine or choline. At 18 d of age, chicks showed curvilinear responses to folic acid supplementation with maximum growth and feed efficiencies at 1.45 mg/kg FA diet. The liver FA response was also curvilinear but reached a plateau at 1.70 mg/kg FA diet. The basal diet for 3 additional experiments contained soybean meal that had been washed with methanol to remove most of the choline. The diet contained only 0.6 mg /kg folic acid and 754 mg /kg choline. Chicks exhibited a larger growth response to folic acid at low choline levels as evidenced by a significant FA x choline interaction. FA supplementation increased but then decreased valgus leg deformity. Choline supplementation also decreased the incidences of valgus and varus leg deformities and decreased bone ash and increased the incidence of tibial dyschondroplasia. It is concluded that chicks fed diets based on practical ingredients require from 1.45 to 1.70 mg /kg FA diet and also 1.60 mg/kg FA when choline is offered near the NRC recommended level of 1,300 mg/kg.

• 한국식품위생안전성학회지
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• 제23권4호
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• pp.338-342
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• 2008

콜린은 정상적인 세포벽의 기능, 아세틸콜린의 합성, 지질의 운송, 그리고 매칠기의 공급원으로서 중요한 유기화합물이다. 콜린의 보충이 콜린성 신경세포의 활성을 통해 학습 및 기억력 향상에 효과가 있다는 연구 보고를 토대로, 시중에서 시판되는 많은 우유제품 속에 콜린 함유량을 증가시켜 뇌를 좋게 하는 기능성 식품으로 판매되고 있다. 따라서 본 연구에서는 효소측정법을 이용하여, 시중에서 판매되고 있는 전유와 첨가우유들을 5개 회사의 제품을 대상으로 콜린의 함량을 분석하였다. 콜린의 표준용액을 이용하여 구한 표준곡선은 0.00316의 기울기와 0.998의 $r^2$ 값을 나타내었다. 회수율은 89.8-97.6%로 양호한 값을 나타내었다. 분석한 우유에서의 콜린함유량은, 전유에서는 14.56-15.19 mg/100 g이었고, 첨가우유에서는 4.11-11.50 mg/100 g을 나타내었다.

• Journal of Nutrition and Health
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• 제23권6호
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• pp.418-426
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• 1990

발암물질인 2-acetylaminofluorence(2-AAF)과 Choline 결핍이 서로 다른 지방을 섭취한 쥐간의 지질과산물, Glucose 6-phosphatase (G6Pase)와 Glutathione S-transferase(GST) 활성도에 미치는 영향을 연구 하였다. 식이 지방은 쇠기름과 옥수수유를 사용하였으며 각 식이 지방군을 Choline 결핍군과 대조군으로 나누어 실험하였다. 식이섭취 3주와 5주째, 2-AAF 처리군과 비처리군으로 나누어 처리군에 매주 2회씩 총4회 2-AAF를 주사 한 뒤 식이섭취 10주 루 동물을 희생 시켰다. MIcrosome의 지질과산화물 함량은 2-AAF와 Choline결핍(CD)식이에 의해 옥수수유 군에서 증가하여 지질과산화 반응에 있어 2-AAF와 CD식이의 역활이 쇠기름을 섭취하였을때 보다 옥수수유를 섭취한 경우 더 중요함을 알 수 있었다. Microsome의 G6Pase활성은 유의적이지는 않으나 2-AAF와 CD식이에 의해 감소하는 경향을 보였으며 옥수수유의 섭취에 의해서는 유의적으로 감소하였다. GST활성은 식이지방에 따라 2-AAF나 CD식이에 의해 증가하였으며 이때 증가된 GST는 발암물질의 대사와 지질과산화물 형성에 대해 방어 작용을 한것으로 보인다.