• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.66-69
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• 2001

Previously we developed a CHO cell line (CHO-OTC1-A19) expressing the first two enzymes of urea cycle. This cell line showed higher ammonia removal activity and faster growth rate than the vector controlled CHO cells (CHO-neo-5). The purpose of this study was to develop a cell line with higher ammonia removal activity than the cell line developed previously. To accomplish this, we constructed stable CHO cell lines expressing the first three, the first four, or all five enzymes of urea cycle by the stable transfection method. We finally selected CHO-AL-19 cell line expressing the first three, the first four enzymes of the cycle with higher ammonia activity than CHO-OTC1-A19 and CHO-n대-5 cell lines: 40% and 15% higher than those of CHO-neo-5 and CHO-OTC1-A19 cell lines 72 hour after culture started, respectively. It also showed 44% and 10% higher cell viability than CHO-neo-5 and CHO- OTC1-A19 cell lines at higher cell density. In addition, CHO-AL-19 cells showed 45%-60% and about 20% lower ammonia concentration per cell than those of CHO-neo-% and CHO-OTC1-A19 cell lines, respectively. These results indicate that CHO-AL-19 could be used in the production of human therapeutic proteins with higher efficiency.

• Journal of architectural history
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• v.9 no.3 s.24
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• pp.37-49
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• 2000

Jeong-Gak is one of the Confucian architecture in Cho-Sun Dynasty. This study is founded on the architectural characteristics and the Confucian order of Jeong-Gak. The Cho-Sun Dynasty had put in operation positive Jeong-Pyo Policy for diffusion Confusion ethics and educate the people. Prize methods of Jeong-Pyo Policy are Jeong-Ryeo, Jeong-moon, remit corvee, present a post and present goods. Jeong-Moon and Jeong-Ryeo is red gate. Vocabularies of Jeong-Moon and Jeong-Ryeo have used mixing but I think that both meaning is different essentially each other. The red gate that elected in front of gate or gateway is Jeong-Moon and another red gate that elected an entrance a village is Jeong-Ryeo. Jeong-Moon and Jeong-Ryeo have no roof, so they are many problem for maintenance and management. Accordingly Jeong-Gak come out from the reign of King Jung-Jong because shelter of Jeong-Moon and Jeong-Ryeo. The function of the Jeong-Gak is shelter of the Jeong-Moon and Jeong-Ryeo, encouragement of Sam-Gang(三綱) ethics, and ostentation of a family. Besides it has a monumental characteristic. It has been distributed the whole country land that Jeong-Gak is closely related with the community of same family (rural community) the latter period of the Cho-Sun Dynasty. The architectural particularity of the Jeong-Gak is brilliance of building materials as compared with different Confucian architecture and appearance of various architectural type. It is that a type of Jeong-Gak appeared Jeong-Ryeo-Gak type, a lofty gate type, and Stone Jeong-Ryeo type. And there are keeping a rule that is to say Confucian order clearly.

• Proceedings of the SAREK Conference
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• 2005.06a
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• pp.141-141
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• 2005

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.10a
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• pp.32-32
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• 2018

• Journal of Microbiology and Biotechnology
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• v.29 no.1
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• pp.55-58
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• 2019

Development of stable rCHO cell lines is still time consuming and labor intensive, although it is a critical step in the commercial development of recombinant antibodies. The current work demonstrates, for the first time, that electroporation of CHO cells with DMSO can enhance stable expression of recombinant antibodies in rCHO cells. Electroporation with DMSO resulted in an average 3.7-fold and 2.8-fold increases in expression levels of aflibercept and pembrolizumab, respectively, in pools of stable rCHO cells. It also resulted in an average of 2.2-fold and 2.6-fold increases in the expression of aflibercept and pembrolizumab, respectively, in single-cell derived rCHO clones. Simple batch cultures of rCHO cell clones with the highest expression produced 1.0 g/l for aflibercept and 1.4 g/l for pembrolizumab without a time-consuming gene amplification process. Electroporation with DMSO also shortened the development of rCHO cell lines to 2-3 months, allowing rapid establishment of stable rCHO cell lines with a desirable expression level antibodies.

• Proceedings of the KIEE Conference
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• 1995.07b
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• pp.682-684
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• 1995

The most effective way to improve the performance of a fuzzy controller may be to optimize look-up values. Look-up values are derived from processes used input-output scale factors, membership functions, rule base, fuzzy inference method and defuzzification. It is powerful way to modify or organize look-up table values. In this paper, We propose the look-up values self-organizing fuzzy controller(LSOFC). We use the plus-minus tuning method(PMTM), scanning values through the processes of addition and subtraction. We show the efficiency of this LSOFC by the results of simulation for nonlinear time-varying plant with unmodelled dynamics.

• The Journal of the Korea Contents Association
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• v.10 no.2
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• pp.444-451
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• 2010

The purpose of this study is about becomes the foundation which establishes the area image integrated strategy the area image and the major factor of the Cho-jeong. For a study result area image integration the considered seriously was self-combustion and creativity, according to demographics quality the possibility of knowing the fact that partially is difference of recognition. This will lead and for an area image integration from and will be natural to use the delegation of a district resources, the image integrated strategy which is discriminated must be established and the possibility.

• Journal of the Korean Solar Energy Society
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• v.25 no.4
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• pp.21-27
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• 2005

This paper summarizes the results of these efforts by offering a photovoltaic system structure in 50kW middle scale applications installed in Cho-sun University dormitory roof. The combination of photovoltaic system components are interconnected and system monitoring system will be summarized for the purpose of the increasing safety in this article. This paper describes configuration of utility interactive photovoltaic system which generated electric power supplies to dormitory. In order to installing the middle or large scale photovoltaic system, It must investigated the optimal design of system, compute quantity of power generation, economic rate of return and so on. In this paper represent 50kW utility photovoltaic system examination and developed simulation results. The performance of photovoltaic system has been evaluated and analyzed with simulation. The results obtained in this research will be much useful to prior investigation for installing utility interactive photovoltaic system.

• Proceedings of the Zoological Society Korea Conference
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• 2000.10a
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• pp.294.3-295
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• 2000

• 한국도로학회:학술대회논문집
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• 2008.03a
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• pp.31-38
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• 2008