• KSBB Journal
• /
• 제21권5호
• /
• pp.325-330
• /
• 2006

Bio-MEMS를 기반으로 microfilter와 백금 전극이 내재되어 있는 microbiochip을 제작하였다. 우리는 이 chip으로 microbead에 indirect ELISA 방법으로 항원-항체를 반응시키고 전기 신호 검출 방법을 이용하여 반응 여부를 판단하였다. 이 때 신호 증폭을 위해 silver enhancer를 사용하였다. Chip 상에서 항원-항체 반응 조건을 최적화하기 위해 pH, temperature, flow time, flow rate, silver enhancer time을 결정하였다. 이렇게 최적화된 조건을 바탕으로 짧은 시간 안에 소량의 시료로 immunoassay를 성공적으로 수행할 수 있었다. 전기 신호 검출 방식을 사용함으로써 biosensor 장비의 소형화와 다중 시료 측정과 자동화를 biosensor에서 적용할 수 있는 가능성을 제시하였다.

• 소성∙가공
• /
• 제15권9호
• /
• pp.667-672
• /
• 2006

Agglutination is one of the most commonly employed reactions in clinical diagnosis. In this paper, we have designed and fabricated nickel mold insert for injection molding of a microfluidic lab-on-a-chip for the purpose of the efficient detection of agglutination. In the presented microfluidic lab-on-a-chip, two inlets for sample blood and reagent, flow guiding microchannels, improved serpentine laminating micromixer(ISLM) and reaction microwells are fully integrated. The ISLM, recently developed by our group, can highly improve mixing of the sample blood and reagent in the microchannel, thereby enhancing reaction of agglutinogens and agglutinins. The reaction microwell was designed to contain large volume of about $25{\mu}l$ of the mixture of sample blood and reagent. The result of agglutination in the reaction microwell could be determined by means of the level of the light transmission. To achieve the cost-effectiveness, the microfluidic lab-on-a-chip was realized by the injection molding of COC(cyclic olefin copolymer) and thermal bonding of two injection molded COC substrates. To define microfeatures in the microfluidic lab-on-a-chip precisely, the nickel mold inserts of lab-on-a-chip for the injection molding were fabricated by combining the UV photolithography with a negative photoresist SU-8 and the nickel electroplating process. The microfluidic lab-on-a-chip developed in this study could be applied to various clinical diagnosis based on agglutination.

• 한국건설순환자원학회논문집
• /
• 제10권3호
• /
• pp.327-334
• /
• 2022

이 연구에서는 목재칩 열병합발전소 바닥재를 잔골재로 활용한 모르타르 및 콘크리트 특성을 평가하고자 부순 잔골재 대용으로서 목재칩 골재대체율과 물시멘트비에 따른 모르타르 특성과 목재칩 골재대체율에 따른 콘크리트의 특성을 비교 및 평가하였다. 목재칩 골재 대체율에 따른 시멘트 모르타르의 플로우는 목재칩 골재 대체율이 높아질수록 증가하는 경향을 보이고, 압축강도와 휨강도는 목재칩 골재 대체율이 높아질수록 증가하였다. 콘크리트의 슬럼프와 공기량은 골재 대체율이 높아질수록 증가하고, 콘크리트의 압축강도와 인장강도는 목재칩 골재 대체율이 증가할수록 높은 경향을 보였다. 이에, 열병합발전소로 인하여 발생하는 바닥재를 콘크리트용 잔골재로 활용하는 방안의 가능성을 확인하였다.

• 대한기계학회논문집B
• /
• 제27권1호
• /
• pp.1-8
• /
• 2003

The heat transfer and flow measurements on a cylindrical pedestal mounted on a flat surface with a turbulent impinging jet were made. The experiments were made for the jet Reynolds number of Re = 23,000, the dimensionless nozzle-to-surface distance of L/d = 2~10, the dimensionless pedestal height of H/D = 0~1.5. Measurements of the surface temperature and the Nusselt number distributions on the plate surface were made using liquid crystal and shroud-transient technique. Flow measurements involve smoke flow visualization and the wall pressure coefficient. The results show that the wall pressure coefficient sharply decreases along the upper surface of the pedestal. However, the pressure increases when the fluid escapes from the pedestal and then collides on the plate surface. The secondary maxima in the Nusselt numbers occur in the region of 1.0 $\leq$ r/d $\leq$ 1.9. Their values for the case of H/D = 0.5 are maximum 80% higher than those for other cases. The formation of the secondary maxima may be attributed to the reattachment of flow on the plate surface which was separated at the edge of the pedestal.

• 한국공작기계학회논문집
• /
• 제16권3호
• /
• pp.50-55
• /
• 2007

The purpose of this research is to enhance the luminance of the LED and to improve the implementation of color by mounting an array lens on the LED without special technology in process. The workmanship of key components considering the economical efficiency and the injection molding technology for high quality of the product are essential to achieve it. In this paper, the mold was computer-aided was designed and manufactured by CAM software (NX4) and high speed machining center. the applied final machining conditions were 3,000-5,000mm/min feed speed, 15,000-25,000rpm and ${\Phi}0.3mm$ ball end-mill. And the Flow analysis was performed using the mold flow software(MPI) in order to get uniformity of resin. Injection conditions acquired by the flow analysis and the injection experiment are as follows. The cylinder temperature is $220-260^{\circ}C$, the mold temperature is $70-80^{\circ}C$, the injection time is about 1.2sec, the injection pressure and velocity is each 7.8-14.7Mpa, and the injection velocity is 0.8-1.2m/sec.

• Korean Chemical Engineering Research
• /
• 제44권5호
• /
• pp.513-519
• /
• 2006

본 연구에서는 유리(glass)와 석영(quartz)을 재질로 사용하여 MEMS(micro-electro mechanical systems) 공정을 통해 전기영동(electrophoresis)을 위한 microchip을 제작하였다. UV 광이 실리콘(silicon)을 투과하지 못하는 점에 착안하여, 다결정 실리콘(polycrystalline Si, poly-Si) 층을 채널 이외의 부분에 증착시킨 광 차단판(optical slit)에 의해 채널에만 집중된 UV 광의 신호/잡음비(signal-to-noise ratio: S/N ratio)를 크게 향상시켰다. Glass chip에서는 증착된 poly-Si 층이 식각 마스크(etch mask)의 역할을 하는 동시에 접합표면을 적절히 형성하여 양극 접합(anodic bonding)을 가능케 하 였다. Quartz 웨이퍼에 비해 불순물을 많이 포함하는 glass 웨이퍼에서는 표면이 거친 채널 내부를 형성하게 되어 시료용액의 미세한 흐름에 영향을 미치게 된다. 이에 따라, HF와 $NH_4F$ 용액에 의한 혼합 식각액(etchant)을 도입하여 표면 거칠기를 감소시켰다. 두 종류의 재질로 제작된 채널의 형태와 크기를 관찰하였고, microchip electrophoresis에 적용한 결과, quartz과 glass chip의 전기삼투 흐름속도(electroosmotic flow velocity)가 0.5와 0.36 mm/s로 측정되었다. Poly-Si 층에 의한 광 차단판의 존재에 의해, peak의 S/N ratio는 quartz chip이 약 2배 수준, glass chip이 약 3배 수준으로 향상되었고, UV 최대흡광 감도는 각각 약 1.6배 및 1.7배 정도 증가하였다.

• 대한기계학회논문집A
• /
• 제30권4호
• /
• pp.373-378
• /
• 2006

We present the design, fabrication, and characterization of a multi-chip microelectrofluidic bench, achieving both electrical and fluidic interconnections with a simple, low-loss and low-temperature electrofluidic interconnection method. We design 4-chip microelectrofluidic bench, having three electrical pads and two fluidic I/O ports. Each device chip, having three electrical interconnections and a pair of two fluidic I/O interconnections, can be assembled to the microelectofluidic bench with electrical and fluidic interconnections. In the fluidic and electrical characterization, we measure the average pressure drop of $13.6{\sim}125.4$ Pa/mm with the nonlinearity of 3.1 % for the flow-rates of $10{\sim}100{\mu}l/min$ in the fluidic line. The pressure drop per fluidic interconnection is measured as 0.19kPa. Experimentally, there are no significant differences in pressure drops between straight channels and elbow channels. The measured average electrical resistance is $0.26{\Omega}/mm$ in the electrical line. The electrical resistance per each electrical interconnection is measured as $0.64{\Omega}$. Mechanically, the maximum pressure, where the microelectrofluidic bench endures, reaches up to $115{\pm}11kPa$.

• KSBB Journal
• /
• 제20권3호
• /
• pp.197-204
• /
• 2005

이상 랩온어칩에서 사용된 생물분리 방법과 그 예를 소개하였다. 랩온어칩에서는 수백 마이크로미터 이하의 미세 채널을 사용하므로 유사한 크기의 채널을 사용하는 capillary electrophoresis에서 사용되었던 기법들이 가장 많이 활용되어왔으며, 랩온어칩 내에서 물질분리를 위한 기본 방법으로 적용되어왔다. 현재까지 CE에 사용되었던 기법들은 모두 랩온어칩 상에 구현된 바 있으며, 이러한 기술들은 랩온어칩의 활용 가능성 및 활용 분야 증대에 크게 기여하였다. 이외에도, laminar flow의 특성을 이용하거나, 막을 제작하거나, 추출 기법을 활용하는 등의 다양한 시도가 있었다. 그러나, high-throughput, 이동형 장비를 지향하는 랩온어칩에서 고전압을 사용하는 경우 활용에 제약을 가져올 수 있어, 용도에 맞는 적절한 분리기술의 개발 및 선택이 랩온어칩의 활용 가능성을 결정짓는 중요한 요인이 될 것으로 판단된다.

• 한국전산유체공학회지
• /
• 제14권4호
• /
• pp.86-92
• /
• 2009

Nowadays, the development of microfluidic chip [i.e. biochip, micro-total analysis system ($\mu$-TAS) and LOC (lab-on-a-chip)] becomes more active, and the microchannels to deliver fluid by pressure or electroosmotic forces tend to be more complex like electronic circuits or networks. For a simple network of channels, we may calculate the pressure and the flow rate easily by using suitable formula. However, for complex network it is not handy to obtain such information with that simple way. For this reason, Graphic User Interface (GUI) program which can rapidly give required information should be necessary for microchip designers. In this paper, we present a GUI program developed in our laboratory and the simple theoretical formula used in the program. We applied our program to simple case and could get results compared well with other numerical results. Further, we applied our program to several complex cases and obtained reasonable results.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2008년도 추계학술대회B
• /
• pp.2696-2699
• /
• 2008

Microfluidic chips have been frequently utilized to perform biochemical analysis, like cell culture, because they reduce the consumptions of analytes and reagents and automate multi-step analysis processes. It is often critical to monitor temperature in a microchannel for the analyses in order to control a reaction condition of bio or chemical molecules. We propose a novel method to monitor temperature of a microchannel flow by using polydiacetylene (PDA), a conjugated polymer, that has a unique property to transform its color from visible blue to fluorescent red by thermal stress. We inject PDA sensor droplets generated by hydrodynamic instability into a microchannel with a microheater incorporated on the channel bottom. Also, we change the channel temperature by providing the different electric power to the microheater. The results show that the florescence intensity of PDA sensor droplets linearly increases in response to the flow temperature increase within a certain range.