KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Bioseparations in Lab-On-A-Chip

랩온어칩에서의 생물분리기술

  • Chang Woo-Jin (Department of Biological Engineering, Inha University) ;
  • Koo Yoon-Mo (Department of Biological Engineering, Inha University, Center for Advanced Bioseparation Technology, Inha University)
  • 장우진 (인하대학교 생물공학과) ;
  • 구윤모 (인하대학교 생물공학과, 초정밀생물분리기술연구센터)
  • Published : 2005.06.01
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Abstract

Lab-on-a-chip is a miniaturized analytical device in which all of the procedures for the analysis of molecules are carried out, such as pretreatment, reaction, separation, detection, etc. Lab-on-a-chip has increasing concern as a device not only for rapid detection of molecules but also for high throughput screening and point of care, because conventional laborious and time consuming analytical procedures can be substituted. Thus, a lot of microfabrication and analytical techniques for lab-on-a-chip have been developed with microstructures smaller than a few hundreds of micrometers. Separation of the molecules is one of the most important components of lab-on-a-chip, because effective separation method can simplify the design and can provide better sensitivity. The electrokinetic separation based on capillary electrophoresis is most widely employed technique in lab-on-a-chip for the control of fluids and the separation of molecules. In this article, bioseparation techniques and its applications realized in lab-on-a-chip are reviewed.

이상 랩온어칩에서 사용된 생물분리 방법과 그 예를 소개하였다. 랩온어칩에서는 수백 마이크로미터 이하의 미세 채널을 사용하므로 유사한 크기의 채널을 사용하는 capillary electrophoresis에서 사용되었던 기법들이 가장 많이 활용되어왔으며, 랩온어칩 내에서 물질분리를 위한 기본 방법으로 적용되어왔다. 현재까지 CE에 사용되었던 기법들은 모두 랩온어칩 상에 구현된 바 있으며, 이러한 기술들은 랩온어칩의 활용 가능성 및 활용 분야 증대에 크게 기여하였다. 이외에도, laminar flow의 특성을 이용하거나, 막을 제작하거나, 추출 기법을 활용하는 등의 다양한 시도가 있었다. 그러나, high-throughput, 이동형 장비를 지향하는 랩온어칩에서 고전압을 사용하는 경우 활용에 제약을 가져올 수 있어, 용도에 맞는 적절한 분리기술의 개발 및 선택이 랩온어칩의 활용 가능성을 결정짓는 중요한 요인이 될 것으로 판단된다.

Keywords

References

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