• KSBB Journal
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• v.21 no.5
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• pp.325-330
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• 2006

This study presents the characterization of an integrated portable microfluidic electrical detection system for fast and low volume immunoassay using polystyrene microbead, which are used as immobilization surfaces. In our chip, a filtration method using the microbead was adopted for sample immobilization and immunogold silver staining(IGSS) was used to increase the electrical signal. The chip is composed of an inexpensive and biocompatible Polydimethylsiloxane(PDMS) layer and Pyrex glass substrate. Platinum microelectrodes for electric signal detection were fabricated on the substrate and microchannel and pillar-type microfilters were formed in the PDMS layer. With a fabricated chip, we reacted antigen and antibody according to the procedures. Then, silver enhancer was injected to increase the size of nanogold particles tagged with the second antibody. As a result, microbeads were connected to each other and formed an electrical bridge between microelectrodes. Resistance measured through the electrodes showed a difference of two orders of magnitude between specific and nonspecific immuno-reactions. The detection limit was 10 ng/ml. The developed immunoassay chip reduced the total analysis time from 3 hours to 50 min. Fast and low-volume biochemical analysis has been successfully achieved with the developed microfilter and immuno-sensor chip, which is integrated to the microfluidic system.

• Transactions of Materials Processing
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• v.15 no.9 s.90
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• pp.667-672
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• 2006

Agglutination is one of the most commonly employed reactions in clinical diagnosis. In this paper, we have designed and fabricated nickel mold insert for injection molding of a microfluidic lab-on-a-chip for the purpose of the efficient detection of agglutination. In the presented microfluidic lab-on-a-chip, two inlets for sample blood and reagent, flow guiding microchannels, improved serpentine laminating micromixer(ISLM) and reaction microwells are fully integrated. The ISLM, recently developed by our group, can highly improve mixing of the sample blood and reagent in the microchannel, thereby enhancing reaction of agglutinogens and agglutinins. The reaction microwell was designed to contain large volume of about $25{\mu}l$ of the mixture of sample blood and reagent. The result of agglutination in the reaction microwell could be determined by means of the level of the light transmission. To achieve the cost-effectiveness, the microfluidic lab-on-a-chip was realized by the injection molding of COC(cyclic olefin copolymer) and thermal bonding of two injection molded COC substrates. To define microfeatures in the microfluidic lab-on-a-chip precisely, the nickel mold inserts of lab-on-a-chip for the injection molding were fabricated by combining the UV photolithography with a negative photoresist SU-8 and the nickel electroplating process. The microfluidic lab-on-a-chip developed in this study could be applied to various clinical diagnosis based on agglutination.

• Journal of the Korean Recycled Construction Resources Institute
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• v.10 no.3
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• pp.327-334
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• 2022

In this study, in order to evaluate the characteristics of mortar and concrete using wood chip cogeneration plant flooring as fine aggregate, mortar characteristics according to wood chip aggregate replacement rate and water-cement ratio as a substitute for crushed sand, and concrete characteristics according to wood chip aggregate replacement rate were compared and evaluated. The cement mortar flow according to the wood chip aggregate replacement rate showed a tendency to increase as the wood chip aggregate replacement rate increased, and the compressive strength and flexural strength increased as the wood chip aggregate replacement rate increased. The slump and air content of concrete increased as the aggregate replacement rate increased, and the compressive strength and tensile splitting strength of concrete tended to increase as the wood chip aggregate replacement rate increased. Accordingly, the possibility of using the flooring by the cogeneration plant as a fine aggregate for concrete was confirmed.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.27 no.1
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• pp.1-8
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• 2003

The heat transfer and flow measurements on a cylindrical pedestal mounted on a flat surface with a turbulent impinging jet were made. The experiments were made for the jet Reynolds number of Re = 23,000, the dimensionless nozzle-to-surface distance of L/d = 2~10, the dimensionless pedestal height of H/D = 0~1.5. Measurements of the surface temperature and the Nusselt number distributions on the plate surface were made using liquid crystal and shroud-transient technique. Flow measurements involve smoke flow visualization and the wall pressure coefficient. The results show that the wall pressure coefficient sharply decreases along the upper surface of the pedestal. However, the pressure increases when the fluid escapes from the pedestal and then collides on the plate surface. The secondary maxima in the Nusselt numbers occur in the region of 1.0 $\leq$ r/d $\leq$ 1.9. Their values for the case of H/D = 0.5 are maximum 80% higher than those for other cases. The formation of the secondary maxima may be attributed to the reattachment of flow on the plate surface which was separated at the edge of the pedestal.

• Transactions of the Korean Society of Machine Tool Engineers
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• v.16 no.3
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• pp.50-55
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• 2007

The purpose of this research is to enhance the luminance of the LED and to improve the implementation of color by mounting an array lens on the LED without special technology in process. The workmanship of key components considering the economical efficiency and the injection molding technology for high quality of the product are essential to achieve it. In this paper, the mold was computer-aided was designed and manufactured by CAM software (NX4) and high speed machining center. the applied final machining conditions were 3,000-5,000mm/min feed speed, 15,000-25,000rpm and ${\Phi}0.3mm$ ball end-mill. And the Flow analysis was performed using the mold flow software(MPI) in order to get uniformity of resin. Injection conditions acquired by the flow analysis and the injection experiment are as follows. The cylinder temperature is $220-260^{\circ}C$, the mold temperature is $70-80^{\circ}C$, the injection time is about 1.2sec, the injection pressure and velocity is each 7.8-14.7Mpa, and the injection velocity is 0.8-1.2m/sec.

• Korean Chemical Engineering Research
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• v.44 no.5
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• pp.513-519
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• 2006

We developed two kinds of the microchip for application to electrophoresis based on both glass and quartz employing the MEMS fabrications. The poly-Si layer deposited onto the bonding interface apart from channel regions can play a role as the optical slit cutting off the stray light in order to concentrate the UV ray, from which it is possible to improve the signal-to-noise (S/N) ratio of the detection on a chip. In the glass chip, the deposited poly-Si layer had an important function of the etch mask and provided the bonding surface properly enabling the anodic bonding. The glass wafer including more impurities than quartz one results in the higher surface roughness of the channel wall, which affects subsequently on the microflow behavior of the sample solutions. In order to solve this problem, we prepared here the mixed etchant consisting HF and $NH_4F$ solutions, by which the surface roughness was reduced. Both the shape and the dimension of each channel were observed, and the electroosmotic flow velocities were measured as 0.5 mm/s for quartz and 0.36 mm/s for glass channel by implementing the microchip electrophoresis. Applying the optical slit with poly-Si layer provides that the S/N ratio of the peak is increased as ca. 2 times for quartz chip and ca. 3 times for glass chip. The maximum UV absorbance is also enhanced with ca. 1.6 and 1.7 times, respectively.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.30 no.4 s.247
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• pp.373-378
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• 2006

We present the design, fabrication, and characterization of a multi-chip microelectrofluidic bench, achieving both electrical and fluidic interconnections with a simple, low-loss and low-temperature electrofluidic interconnection method. We design 4-chip microelectrofluidic bench, having three electrical pads and two fluidic I/O ports. Each device chip, having three electrical interconnections and a pair of two fluidic I/O interconnections, can be assembled to the microelectofluidic bench with electrical and fluidic interconnections. In the fluidic and electrical characterization, we measure the average pressure drop of $13.6{\sim}125.4$ Pa/mm with the nonlinearity of 3.1 % for the flow-rates of $10{\sim}100{\mu}l/min$ in the fluidic line. The pressure drop per fluidic interconnection is measured as 0.19kPa. Experimentally, there are no significant differences in pressure drops between straight channels and elbow channels. The measured average electrical resistance is $0.26{\Omega}/mm$ in the electrical line. The electrical resistance per each electrical interconnection is measured as $0.64{\Omega}$. Mechanically, the maximum pressure, where the microelectrofluidic bench endures, reaches up to $115{\pm}11kPa$.

• KSBB Journal
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• v.20 no.3
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• pp.197-204
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• 2005

Lab-on-a-chip is a miniaturized analytical device in which all of the procedures for the analysis of molecules are carried out, such as pretreatment, reaction, separation, detection, etc. Lab-on-a-chip has increasing concern as a device not only for rapid detection of molecules but also for high throughput screening and point of care, because conventional laborious and time consuming analytical procedures can be substituted. Thus, a lot of microfabrication and analytical techniques for lab-on-a-chip have been developed with microstructures smaller than a few hundreds of micrometers. Separation of the molecules is one of the most important components of lab-on-a-chip, because effective separation method can simplify the design and can provide better sensitivity. The electrokinetic separation based on capillary electrophoresis is most widely employed technique in lab-on-a-chip for the control of fluids and the separation of molecules. In this article, bioseparation techniques and its applications realized in lab-on-a-chip are reviewed.

• Journal of computational fluids engineering
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• v.14 no.4
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• pp.86-92
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• 2009

Nowadays, the development of microfluidic chip [i.e. biochip, micro-total analysis system ($\mu$-TAS) and LOC (lab-on-a-chip)] becomes more active, and the microchannels to deliver fluid by pressure or electroosmotic forces tend to be more complex like electronic circuits or networks. For a simple network of channels, we may calculate the pressure and the flow rate easily by using suitable formula. However, for complex network it is not handy to obtain such information with that simple way. For this reason, Graphic User Interface (GUI) program which can rapidly give required information should be necessary for microchip designers. In this paper, we present a GUI program developed in our laboratory and the simple theoretical formula used in the program. We applied our program to simple case and could get results compared well with other numerical results. Further, we applied our program to several complex cases and obtained reasonable results.

• Proceedings of the KSME Conference
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• 2008.11b
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• pp.2696-2699
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• 2008

Microfluidic chips have been frequently utilized to perform biochemical analysis, like cell culture, because they reduce the consumptions of analytes and reagents and automate multi-step analysis processes. It is often critical to monitor temperature in a microchannel for the analyses in order to control a reaction condition of bio or chemical molecules. We propose a novel method to monitor temperature of a microchannel flow by using polydiacetylene (PDA), a conjugated polymer, that has a unique property to transform its color from visible blue to fluorescent red by thermal stress. We inject PDA sensor droplets generated by hydrodynamic instability into a microchannel with a microheater incorporated on the channel bottom. Also, we change the channel temperature by providing the different electric power to the microheater. The results show that the florescence intensity of PDA sensor droplets linearly increases in response to the flow temperature increase within a certain range.