• Journal of Ginseng Research
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• 제24권3호
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• pp.148-152
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• 2000

한국홍삼과 중국홍삼의 향 유형 및 강도를 관능적으로 비교한 결과 한국홍삼은 단 냄새와 구수한 냄새가 강한 반면에 중국홍삼은 흙 냄새, 나무냄새 및 건초냄새가 강한 경향이었다. 한국홍삼의 구수한 냄새와 단 냄새는 헤드스페이스추출물의 에테르분획, 그 중에서도 중성부에서 강하게 감지되었다.

• Journal of Ginseng Research
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• 제44권3호
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• pp.506-518
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• 2020

Background: Red-skin root of Asian ginseng (Panax ginseng) significantly reduces the quality and limits the production of ginseng in China. The disease has long been thought to be a noninfectious physiological disease, except one report that proved it was an infectious disease. However, the causal agents have not been successfully determined. In the present study, we were to reveal the pathogens that cause red-skin disease. Methods: Ginseng roots with red-skin root symptoms were collected from commercial fields in Northeast China. Fungi were isolated from the lesion and identified based on morphological characters along with multilocus sequence analyses on internal transcription spacer, β-tubulin (tub2), histone H3 (his3), and translation elongation factor 1α (tef-1α). Pathogens were confirmed by inoculating the isolates in ginseng roots. Results: A total of 230 isolates were obtained from 209 disease samples. These isolates were classified into 12 species, including Dactylonectria sp., D. hordeicola, Fusarium acuminatum, F. avenaceum, F. solani, F. torulosum, Ilyonectria mors-panacis, I. robusta, Rhexocercosporidium panacis, and three novel species I. changbaiensis, I. communis, and I. qitaiheensis. Among them, I. communis, I. robusta, and F. solani had the highest isolation frequencies, being 36.1%, 20.9%, and 23.9%, respectively. All these species isolated were pathogenic to ginseng roots and caused red-skin root disease under appropriate condition. Conclusion: Fungal complex is the causal agent of red-skin root in P. ginseng.

• Journal of Ginseng Research
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• 제46권2호
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• pp.290-295
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• 2022

Background: Dried and red ginseng are well-known types of processed ginseng and are widely used as healthy food. The dried and red ginseng quality may vary with the storage period of raw ginseng. Therefore, herein, the effect of the storage period of fresh ginseng on processed ginseng quality was evaluated through multicomponent quantification with statistical analysis. Methods: A method based on ultrahigh performance liquid chromatography coupled to triple quadrupole mass spectrometry in multiple-reaction monitoring mode (UPLC-MRM-MS) was developed for quantitation of ginsenosides and oligosaccharides in dried and red ginseng. Principal component analysis and partial least squares discriminant analysis were conducted to evaluate the dynamic distributions of ginsenosides and oligosaccharides after different storage periods. Results: Eighteen PPD, PPT and OLE ginsenosides and nine reducing and nonreducing oligosaccharides were identified and quantified. With storage period extension, the ginsenoside content in the processed ginseng increased slightly in the first 2 weeks and decreased gradually in the following 9 weeks. The content of reducing oligosaccharides decreased continuously as storage time extending, while that of the nonreducing oligosaccharides increased. Chemical conversions occurred during storage, based on which potential chemical markers for the storage period evaluation of fresh ginseng were screened. Conclusion: According to ginsenoside and oligosaccharide distributions, it was found that the optimal storage period was 2 weeks and that the storage period of fresh ginseng should not exceed 4 weeks at 0 ℃. This study provides deep insights into the quality control of processed ginseng and comprehensive factors for storage of raw ginseng.

• Journal of Ginseng Research
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• 제43권1호
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• pp.27-37
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• 2019

Background: The structural conversions in ginsenosides induced by steaming or heating or acidic condition could improve red ginseng bioactivities significantly. In this paper, the chemical transformations of red American ginseng from fresh Panax quinquefolium L. under steaming were investigated, and the possible mechanisms were discussed. Methods: A method with reversed-phase high-performance liquid chromatography coupled with linear ion trap mass spectrometry ($HPLC-MS^n$)-equipped electrospray ionization ion source was developed for structural analysis and quantitation of ginsenosides in dried and red American ginseng. Results: In total, 59 ginsenosides of protopanaxadiol, protopanaxatriol, oleanane, and ocotillol types were identified in American ginseng before and after steaming process by matching the molecular weight and/or comparing $MS^n$ fragmentation with that of standards and/or known published compounds, and some of them were determined to be disappeared or newly generated under different steaming time and temperature. The specific fragments of each aglycone-type ginsenosides were determined as well as aglycone hydrated and dehydrated ones. The mechanisms were deduced as hydrolysis, hydration, dehydration, and isomerization of neutral and acidic ginsenosides. Furthermore, the relative peak areas of detected compounds were calculated based on peak areas ratio. Conclusion: The multicomponent assessment of American ginseng was conducted by $HPLC-MS^n$. The result is expected to provide possibility for holistic evaluation of the processing procedures of red American ginseng and a scientific basis for the usage of American ginseng in prescription.

• Journal of Ginseng Research
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• 제26권4호
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• pp.196-201
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• 2002

본 연구는 각국 홍삼의 성분비교 연구로서 한국홍삼(찬삼, 지삼, 양삼), 북한홍삼(천삼, 지삼, 양삼), 중국석주홍삼(1등, 2등, 3등), 중국길림홍삼(1등, 2등, 3등), 일본운주홍삼(1등, 2등, 3등) 및 일본신주홍삼(1등, 2등, 3등) 등을 대상으로 사포닌 및 무기물 함량에 대해서 비교 분석한 결과는 다음과 같다. 조사포닌 함량은 한국홍삼의 경우 3.05~3.76%, 북한홍삼은 2.09~3.21% 중국석주홍삼은 2.82~3.71%, 중국길림홍삼은 2.72~3.62%, 일본주홍삼은 2.11~2.44% 그리고 일본신주홍삼은 2.18~2.87%로 전반적으로 한국홍삼이 높게 나타났다. Ginsenoside중에 Rb1, Bb2, Rc, Rd, Re, Rg$_{1}$ 함량을 분석한 결과, 한국홍삼의 경우 약 1.43%로 북한홍삼 1.14%, 중국석주 1.13%, 중국길림 1.07%, 일본운주 0.95%, 일본신주 1.10% 보다 한국홍삼이 약 0.52~0.33% 더 많이 함유하고 있다. 특히 ginsenoside 중에서 Rb$_{1}$, Re, Rg$_{1}$ 성분이 대체적으로 한국홍삼에 많이 함유하고 있었으며, 그 외 성분은 비슷한 경향으로 나타났다. 26종의 무기물함량을 한국홍삼과 타국홍삼과 비교 조사한 결과, 차이는 큰 원소는 As, Ce, Sb, Sm, Sr, K, La, Na 등이었으며, 특히 La성분이 한국홍삼은 0.64~0.88% ppm, 북한홍삼은 0.43~0.67 ppmdldjTdmsk 중국홍삼(석주삼, 길림삼)은 0.09~0.21 ppm 정도이고, 일본홍삼은 0.04~0.14 ppm이었다. 그리고 Na함량에 있어서 한국홍삼은 286~350 ppm인데 비해서 중국홍삼은 59~180 ppm으로 상당히 낮았으며, Sn함량도 한국홍삼은 10.00~72.65 ppm이었으나 중국홍삼은 3.75~9.71 ppm이었다. Brgkafid에 있어서는 일본홍삼이 2.10~3.56 ppm으로 나타났으나 한국홍삼은 0.89~2.77 ppm, 중국홍삼은 0.42~1.04 ppm이었고 Ce함량은 일본홍삼이 가장 낮았다.

• 대한한방내과학회지
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• 제29권4호
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• pp.1075-1082
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• 2008

Objectives : The purpose of this experiment is comparing the difference on natural killer cell activity through Korean red ginseng and Chinese red ginseng by $^{51}Cr$ release assay. Methods : Thirty rats were equally divided into a Korean red ginseng group, a Chinese red ginseng group and a control group. Korean and Chinese red ginseng were administrated to the rats at 200mg daily for a weak, while 0.9% normal saline was given to the control. Percent specific lysis (PSL) and lytic units (LU) were calculated from spleen cells by $^{51}Cr$ release assay. Results : Percent specific lysis of the Korean red ginseng group was significantly higher than that of the control in the ratio of 100:1, effector cell:target cell (p<0.05). Percent specific lysis of Korean red ginseng group was also significantly higher than that of the Chinese red ginseng group in the ratio of 25:1, effector cell:target cell (p<0.05). Chinese red ginseng showed no effect on NK cell activity. Conclusions : These findings suggest that Korean red ginseng improves immunologic function and shows superior effects than Chinese red ginseng.

• 대한한방내과학회지
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• 제28권4호
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• pp.886-895
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• 2007

Objective : The immune system is a complex of systems, all of which work together to clear infection from the body. In Korea, red ginsenghas been one of the herbs most widely used to enhance the immune system for thousand of years. More recently, red ginseng has been reported to have many positive effects on the immune system. The purpose of this study was evaluate the effects of Korean red ginseng and Chinese red ginseng on IgG, IgM, and IgA, using immunoglobulin productivity assay. Methods : Male SD rats were separated into 3 groups. We administered Korean red ginseng (KRG) to one group and Chinese red ginseng (CRG) to another, with normal saline for the Control group consecutively and orally for 3 months. The dose of red ginseng was 500mg per day, as a powder with soluble water. Immunoglobulin levels from spleen cell were estimated by ELISA kit. Results : In immunoglobulin productivity assay (cell), the IgG level of the KRG group significantly increased but there was no significant difference in the IgG of the CRG group. The IgM level of the KRG group significantly increased stimulated with PWM. When it was unstimulated, the level of IgM in KRG and CRG increased together. The IgA level of the KRG group significantly increased when it was stimulated with PWM and unstimulated. Conclusion : According to the above results, oral administration of red ginseng for 3 months is considered useful for immunomodulatory effect, and Korean red ginseng may be superior to Chinese red ginseng in that effect.

• Journal of Ginseng Research
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• 제21권3호
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• pp.196-200
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• 1997

The headspace volatiles of the Korean ginseng and the Chinese ginseng were extracted using the SepPak Cl8 cartridge (Wasters Co.) and were analyzed using GC/MSD. The overall GC pattern of the headspace volatiles of the Chinese ginseng was similar to that of the Korean ginseng, but the composition ratios of the two major components, $\beta$-panasinsene to $\beta$-muurolene, were quite different between them. The composition ratios of $\beta$-panasinsene to $\beta$-muurolene of the Korean red and white ginseng were 1.02$\pm$0.28 (n=19) and 1.49$\pm$0.55 (n=14) , respectively. However the com- position ratios of the Chinese red and dried ginseng were 0.58$\pm$0.19 (n=41) and 0.57$\pm$0.17 (n=28), repetitively, which were significantly lower than those of the Korean ginseng at I% level. The composition ratio of the two major headspace volatile components, $\beta$-panasinsene to ${\gamma}$-muurolene, is thought to be as a useful indicator for differentiating the Chinese ginseng with the Korean ginseng.

• 한국응용과학기술학회지
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• 제37권1호
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• pp.38-48
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• 2020

중국산과 국내산 홍삼 농축액의 혼합비율에 따는 원산지 판별 가능성을 검토하기 위해 전자코를 이용하여 향기 패턴을 분석하였다. 중국산 홍삼 농축액과 국내산 홍삼 농축액의 원산지 판별이 가능하였고 중국산 홍삼농축액의 혼합비율이 증가할수록 검출되어지는 향기 성분의 패턴은 감소하는 경향을 나타내었다. Frequency pattern, derivative pattern을 Vapor print^TM 으로 도형화 하여 비교한 결과 서로 다른 패턴을 보여주어 중국산 홍삼 농축액 첨가비율에 따른 차이는 물론 원산지의 차이도 뚜렷하게 나타났다.

• Journal of Ginseng Research
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• 제22권1호
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• pp.18-21
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• 1998

Comparative cytotoxic activities of petroleum ether soluble fraction from various ginsengs of Panax species were evaluated using A549 (human lung adenocarcinoma) and SK-OV-3(human ovary carcinoma) cancer cell lines. Korean red ginseng, Korean white ginseng, American ginseng and Canadian ginseng were found to show more potent cytotoxicitles on A549 and SK-OV-3 cell lines than Chinese red ginseng, Japanese red ginseng and Sanchi ginseng. It is noteworthy that especially, red ginseng prepared from the root of Panax ginseng cultivated in Korea shows relatively stronger cytotoxic activities than those cultivated in China and Japan.