• Proceedings of the Plant Resources Society of Korea Conference
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• 1993.08a
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• pp.26-31
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• 1993

• Korean Journal of Plant Resources
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• v.7 no.1
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• pp.7-12
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• 1994

• KOREAN JOURNAL OF CROP SCIENCE
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• v.44 no.3
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• pp.221-224
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• 1999

A field experiment was carried out to observe the weed control effects of vetch and to evaluate vetch characteristics on clay loam soil in no-till direct-sown rice-vetch cropping systems. The effects of weed control, forage productivity, and N content of vetch plants were investigated. With the progress of plant growth, density of Chinese milk vetch (Astragalus sinicus L.) gradually decreased, but densities of foxtail and other weeds decreased steeply due to the depression by the over-shaded vetch canopy in a no-till direct-sown rice-vetch cropping paddy field. The vetch density in tillage systems was lower than in notillage cropping systems. Lower vetch density occurred with an increase in foxtail density and other weeds. Weed control effect increased by the progress of vetch growth, which indicated that the vetch canopy over-shaded the weeds. Vetch straw was degraded rapidly submergence after with water at the time of wet sowing of rice. Early harvesting of vetch seed resulted in lower seed germination. To acquire enough seedlings without re-sowing, the harvesting of seed should be delayed at least 28 days after the flowering stage in order to ensure the vetch population is sustainable in a no-till direct-sown rice-vetch cropping system. In order to improve the survival of vetch plants, vetch seeds should germinate from the heading .stage to before the full-ripening stage of rice plants. To enhance the percent of over-wintering survival, vetch seeds should germinate no later than the end of October in southern Korea. The dry weight of vetch plants increased with the progress of vetch growth until the flowering stage but N content decreased for 30 days from before the flowering stage (2.9%) until the ripening stage (1.8%). We concluded that Chinese milk vetch could have an effect on weed control before the flowering stage, sustainability without re-sowing of seed annually, and effective green manure for rice pre-crop in no-till direct-sown rice-vetch relay cropping systems.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2023.04a
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• pp.52-52
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• 2023

Ageing has been known to be deeply related to oxidative activities. Furthermore, inflammation is a response initiated by conditions such as infection and injury. It has been known that complex connections occur between oxidative stress and the inflammatory response, however underlying mechanism needs to be investigated. Chinese resource plants have been widely used as functional food and medicine for a long time, however it is not studied well how many of these resource plants work. We first decided testing anti-oxidant and anti-inflammatory activities with 95% ethanol extracts of the chinese resource plants, Boehmeria pilosinscula (Blume) Hassk., Carlemannia tetragona Hook f. and Clausena emarginata C.C.Huang. To measure anti-oxidant activity, we performed DPPH assay in Raw264.7 cells with 95% ethanol extracts. Clausena extracts showed significantly higher anti-oxidant activity than those of Boehmeria and Carlemannia. We then performed Nitric Oxide assay to measure the inflammation suppression levels with 95% ethanol extracts of these plants. Clausena extracts showed significantly higher suppression of nitric oxide production than those of Boehmeria and Carlemannia. This indicates that inflammation levels are significantly reduced by Clausena. After measuring anti-oxidant and anti-inflammatory activities, we then performed MTT assay to measure the cell survival rate of Raw264.7 cells treated with these extracts. Boehmeria showed much more cell survival rate than Carlemannia and Clausena. Taken together, this result suggests that Clausena extracts have more anti-oxidant and antiinflammatory activities than Boehmeria and Carlemannia, while Boehmeria extracts have more cell survival rate than Carlemannia and Clausena.

• Research in Plant Disease
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• v.24 no.1
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• pp.81-85
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• 2018

Chinese artichoke (Stachys sieboldii Miq.) belongs to herbaceous perennial plants of Labiatea and is cultivated as edible and medicinal crops in China, Japan and Korea. A Chinese artichoke plant showing virus-like symptoms was collected in Chungju, Korea. Plant sap of the sample was inoculated in Nicotiana tabacum cv. Xanthi-nc, Chenopodium quinoa and Chenopodium amaranticolor. Necrotic local lesions were observed in the inoculated leaves of N. tabacum cv. Xanthi-nc and C. amaranticolor, C. quinoa with systemic chlorotic spots and mosaic symptoms on the upper leaves. The disease reactions on indicator plants suggested that the collected Chinese artichoke sample was mixed-infected with different viruses. We detected three viruses by RT-PCR analysis using genus- and species-specific primer sets for Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV) and Tobacco mosaic virus (TMV). This study is the first report of a mixed infection of three viruses in Chinese artichoke in Korea.

• The Plant Pathology Journal
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• v.36 no.2
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• pp.170-178
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• 2020

The Lily mottle virus (LMoV) impedes the growth and quality of lily crops in Lanzhou, China. Recently Arabis mosaic virus (ArMV) has been detected in LMoV-infected plants in this region, causing plant stunting as well as severe foliar symptoms, and likely posing a threat to lily production. Consequently, there is a need to develop simple, sensitive, and reliable detection methods for these two viruses to prevent them from spreading. Reverse transcription (RT) loop-mediated isothermal amplification (LAMP) assays have been developed to detect LMoV and ArMV using two primer pairs that match six conserved sequences of LMoV and ArMV coat proteins, respectively. RT-LAMP assay results were visually assessed in reaction tubes using green fluorescence and gel electrophoresis. Our assays successfully detected both LMoV and ArMV in lily plants without the occurrence of viral cross-reactivity from other lily viruses. Optimal conditions for LAMP reactions were 65℃ and 60℃ for 60 min for LMoV and ArMV, respectively. Detection sensitivity for both RT-LAMP assays was a hundredfold greater than that of our comparative RT-polymerase chain reaction assays. We have also found this relatively rapid, target specific and sensitive method can also be used for samples collected in the field and may be especially useful in regions with limited or no laboratory facilities.

• Korean Journal of Organic Agriculture
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• v.23 no.4
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• pp.911-921
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• 2015

We evaluated two herbal plants, basil (Ocimum basilicum) and lemon balm (Melissa officinalis), for their potential as repellent plants in the management of striped flea beetle (Phyllotreta striolata), in organic production of chinese cabbage. It was confirmed that striped flea beetle avoided these two herbal plants in olfactometer experiment. We conducted field experiment in which the suppressive effect of the two selected herbal plants and cruciferous crops preferred by striped flea beetle were evaluated through cultivating as inter crops and border crops, respectively. In inter-cropping experiment, unlike in vitro test, the density of striped flea beetle was low in basil- or mustard-intercropping cultivation plot, but high in lemon balm-intercropping cultivation plot. In border cropping experiment, the density of striped flea beetle on chinese cabbage in lemon balm or basil cultivation plot were approximately zero. As a result of this study, we think that border cropping of lemon balm or basil can be used to reduce preventively striped flea beetle of chinese cabbage under organic farming system.

• The Plant Pathology Journal
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• v.16 no.5
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• pp.280-282
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• 2000

A leaf spot disease occurred on Allium tuberosum Roth. (Chinese chive) in Korea. All the isolates of Stemphylium sp. from the lesions of the diseased plant parts were identified as S. vesicarium (Waller.) Simmons, based on the morphological characteristics of conidia and conidiophores. Pathogenicity of the fungus was proved by artificial inoculation on Chinese chive plants. This is the first record of leaf blight on Chinese chive caused by S. vesicarium in Korea.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.6
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• pp.465-470
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• 2007

To evaluate the effect of companion plant (Phlox subulata and Glechoma hederacea var. longituba) on conservation of soil in slopped highland, coverage of crops and soil erosion were investigated with 3 different conditions. The coverage rate of Phlox subulata with Chinese cabbage cultivation was kept on approximately 100% from Chinese cabbage-transplanting to -harvest. The coverage rate of Glechoma hederacea var. longituba with Chinese cabbage cultivation was low at it's early stage, however, reached to approximately 100% at the time of Chinese cabbage harvest. In contrast, the coverage rate of Chinese cabbage cultivation without cover crop, and simultaneous transplanting with Chinese cabbage and cover crop were approximately 60%. Losses of soil and nitrate nitrogen were much lower in Phlox subulata with Chinese cabbage cultivation ($0.1{\sim}0.2ton\;ha^{-1}$, $0.2{\sim}0.4kg\;NO_3{^-}-N\;ha^{-1}$) than those in Chinese cabbage cultivation without cover crop ($20.8ton\;ha^{-1}$, $2.1kg\;NO_3{^-}-N\;ha^{-1}$), and simultaneous transplanting with Chinese cabbage and cover crop ($8.9{\sim}10.5ton\;ha^{-1}$, $1.5{\sim}2.2kg\;NO_3{^-}-N\;ha^{-1}$). Cover plants suppressed the weed occurrence up to about 70%. Live mulching with cover plants set a good effects on weed suppression and reduction of soil and nutrient loss. Therefore intercropping with Phlox subulata will make great contributions to soil conservation in Chinese cabbage cultivated highland.

• Plant Biotechnology Reports
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• v.5 no.2
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• pp.187-195
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• 2011

The gesneriaceous perennial plant Titanotrichum oldhamii has beautiful foliage and attractive bright yellow flowers. However, breeding of T. oldhamii by conventional sexual hybridization may be difficult because sexual reproduction of this species is very rare. In the present study, plant regeneration systems via both direct and indirect formation of adventitious shoots from leaf explants were established as the first step toward breeding T. oldhamii by using biotechnological techniques. Adventitious shoots were formed efficiently on medium containing $0.1mg\;l^{-1}$ benzyladenine. Histological observation showed that shoot formation on this medium occurred directly from leaf epidermal cells without callus formation. On the other hand, leaf explants formed calluses on medium containing $0.1mg\;l^{-1}$ 2,4-dichlorophenoxyacetic acid. The calluses could be maintained by monthly subculturing to fresh medium of the same composition. When the calluses were transferred to plant growth regulator-free medium, they formed adventitious shoots. Directly and indirectly formed shoots rooted well on medium containing $0.1mg\;l^{-1}$ indole-3-butyric acid. Plantlets thus obtained were successfully acclimatized and grew vigorously in the greenhouse. Flow cytometry analysis indicated that no variation in the ploidy level was observed in plants regenerated via direct shoot formation, whereas chromosome doubling occurred in several plants regenerated via indirect shoot formation. Regenerated plants with the same ploidy level as the mother plants showed almost the same phenotype as the mother plants, whereas chromosome-doubled plants showed apparent morphological alterations: they had small and crispate flowers, and round and deep green leaves.