식물병연구 (Research in Plant Disease)

한국식물병리학회 (The Korean Society of Plant Pathology)
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국내 초석잠에 복합 감염된 3종 바이러스의 분리 및 동정

Detection and Identification of a Mixed Infection of Three Viruses in Chinese Artichoke in Korea

  • 김은경 (강원대학교 응용생물학과) ;
  • 유재원 (강원대학교 응용생물학과) ;
  • 박지수 (강원대학교 응용생물학과) ;
  • 민동주 (강원대학교 응용생물학과) ;
  • 박석현 (강원대학교 응용생물학과) ;
  • 홍진성 (강원대학교 응용생물학과)
  • Kim, Eun-Kyoung (Department of Applied Biology, Kangwon National University) ;
  • You, Jae-Won (Department of Applied Biology, Kangwon National University) ;
  • Park, Ji-Soo (Department of Applied Biology, Kangwon National University) ;
  • Min, Dong-Joo (Department of Applied Biology, Kangwon National University) ;
  • Park, Suk-Hyun (Department of Applied Biology, Kangwon National University) ;
  • Hong, Jin-Sung (Department of Applied Biology, Kangwon National University)
  • 투고 : 2017.11.03
  • 심사 : 2017.12.04
  • 발행 : 2018.03.31
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초록

2016년 충북 충주에서 모자이크 병징을 보이는 초석잠을 채집하였다. 담배와 명아주에 즙액접종을 실시한 결과 담배에서 접종엽에 괴사반점이 나타나고 명아주 2종에서 상엽에 퇴록반점을 동반한 모자이크 병징이 관찰되었다. 기주반응과 결과를 통해 여러 바이러스의 복합감염을 추정할 수 있었고, 추정되는 바이러스의 확인을 위해 속특이 및 종특이 프라이머를 이용하여 RT-PCR을 수행하여 3종의 바이러스(AMV, CMV, TMV)를 검정하였다. 본 연구는 국내 초석잠에서 3종의 바이러스 감염을 확인하였고 CMV의 감염에 대한 첫 보고이다.

Chinese artichoke (Stachys sieboldii Miq.) belongs to herbaceous perennial plants of Labiatea and is cultivated as edible and medicinal crops in China, Japan and Korea. A Chinese artichoke plant showing virus-like symptoms was collected in Chungju, Korea. Plant sap of the sample was inoculated in Nicotiana tabacum cv. Xanthi-nc, Chenopodium quinoa and Chenopodium amaranticolor. Necrotic local lesions were observed in the inoculated leaves of N. tabacum cv. Xanthi-nc and C. amaranticolor, C. quinoa with systemic chlorotic spots and mosaic symptoms on the upper leaves. The disease reactions on indicator plants suggested that the collected Chinese artichoke sample was mixed-infected with different viruses. We detected three viruses by RT-PCR analysis using genus- and species-specific primer sets for Alfalfa mosaic virus (AMV), Cucumber mosaic virus (CMV) and Tobacco mosaic virus (TMV). This study is the first report of a mixed infection of three viruses in Chinese artichoke in Korea.

키워드

참고문헌

  1. Back, H. S., Na, Y. S., Kim, D. H., Lee, C. H., Lyu, B. H. and Song, S. K. 2004. Antioxidant activities of Stachys sieboldii MIQ roots. J. Life Sci. 14: 1-7. https://doi.org/10.5352/JLS.2004.14.1.001
  2. Broadbent, L. 1965. The epidemiology of tomato mosaic. Ann. Appl. Biol. 56: 177-205. https://doi.org/10.1111/j.1744-7348.1965.tb01227.x
  3. Choi, J. K., Kwon, S. B., Lee, S. Y. and Park, W. M. 1990. Some properties of two isolates of Cucumber mosaic virus isolated from Aster yomena Makino and Commelina communis L. Korean J. Plant Pathol. 6: 138-143.
  4. Choi, S. K., Choi, J. K., Park, W. M. and Ryu, K. H. 1999. RT-PCR detection and identification of three species of cucumoviruses with a genus-specific single pair of primers. J. Virol. Methods 83: 67-73.
  5. Douine, L., Quiot, J. B., Marchoux, G. and Archange, P. 1979. Recensement des especes vegetales sensibles au Virus de la Mosaique du Concombre (CMV) Etude bibliographique. Ann. Phytopathol. 11: 439-475.
  6. Francki, R. I. B., Mossop, D. W. and Hatta, T. 1979. Cucumber mosaic virus. In: CMI/AAB Descriptions of Plant Viruses, 213 pp.
  7. Fuji, S., Yamamoto, H., Furuya, H. and Naito, H. 2003. Characterization of a new potyvirus isolated from Chinese artichoke in Japan. Arch. Virol. 148: 2249-2255. https://doi.org/10.1007/s00705-003-0169-7
  8. Hollings, M. and Huttinga, H. 1976. Tomato mosaic virus. In: CMI/AAB Descriptions of plant viruses, 156 pp.
  9. Hiruki, C. and Hampton, R. O. 1990. Diseases caused by viruses and viruses infectious to alfalfa. In: Compendium of Alfalfa Diseases, pp. 51-58. American Phytopathological Society.
  10. Kaper, J. M. and Waterworth, H. E. 1981. Cucummoviruses. In: Handbook of Plant Virus Infections: Comparative Diagnosis.
  11. Kim, J. S., Lee, S. H., Choi, H. S., Kim, M. K., Kwak, H. R., Kim, J. S. and Choi, G. S. 2012. 2007-2011 characteristics of plant virus infections on crop samples submitted from agricultural places. Res. Plant Dis. 18: 277-289. (In Korean) https://doi.org/10.5423/RPD.2012.18.4.277
  12. Letschert, B., Adam, G., Lesemann, D. E., Willingmann, P. and Heinze, C. 2002. Detection and differentiation of serologically crossreacting tobamoviruses of economical importance by RT-PCR and RT-PCR-RFLP. J. Virol. Methods 106: 1-10. https://doi.org/10.1016/S0166-0934(02)00135-0
  13. Lirong, C. M. Z. G. J. and Jiyan, Q. 1986. Meristem-tip culture and value of application of virus-free clone in Chinese artichoke. Acta Agric. Bor. Sin., 2: 15-22.
  14. Mueller, E. E. and Grau, C. R. 2007. Seasonal progression, symptom development, and yield effects of Alfalfa mosaic virus epidemics on soybean in Wisconsin. Plant Dis. 91: 266-272. https://doi.org/10.1094/PDIS-91-3-0266
  15. Owen, J. and Palukaitis, P. 1988. Characterization of Cucumber mosaic virus I. Molecular heterogeneity mapping of RNA 3 in eight CMV strains. Virology 166: 495-502. https://doi.org/10.1016/0042-6822(88)90520-X
  16. Palukaitis, P. and Garcia-Arenal, F. 2003. Cucumoviruses. Adv. Virus Res. 62: 241-323.
  17. Taylor, R. H., Grogan, R. G. and Kimble, K. A. 1961. Transmission of tobacco mosaic viras in tomato seed. Phytopathology 51: 837-42.
  18. Untiveros, M., Perez-Egusquiza, Z. and Clover, G. 2010. PCR assays for the detection of members of the genus Ilarvirus and family Bromoviridae. J. Virol. Methods 165: 97-104. https://doi.org/10.1016/j.jviromet.2010.01.011
  19. Zheng, L., Rodoni, B. C., Gibbs, M. J. and Gibbs, A. J. 2010. A novel pair of universal primers for the detection of potyviruses. Plant Pathol. 59: 211-220. https://doi.org/10.1111/j.1365-3059.2009.02201.x