• BMB Reports
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• v.39 no.4
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• pp.346-354
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• 2006

The full-length cDNA of grass carp (Ctenopharyngodon idellus) and silver carp (Hypophthalmichthys molitrix) uncoupling protein 2 (UCP2) was obtained from liver. The grass carp UCP2 cDNA was determined to be 1152 bp in length with an open reading frame that encodes 310 amino acids. Five introns (Intron 3, 4, 5, 6 and 7) in the translated region, and partial sequence of Intron 2 in the untranslated region of grass carp UCP2 gene were also obtained. Gene structure comparison between grass carp and mammalian (human and mouse) UCP2 gene shows that, the UCP2 gene structure of grass carp is much similar to that of human and mouse. Partial UCP2 cDNA sequences of bighead carp (Aristichthys nobilis) and mud carp (Cirrhinus molitorella), were further determined. Together with the common carp (Cyprinus carpio) UCP2 sequence from GenBank (AJ243486), multiple alignment result shows that the nucleotide and amino acid sequences of the UCP2 gene, were highly conserved among the five major Chinese carps that belong to four subfamilies. Using beta-actin as control, the ratio UCP2/beta-actin mRNA (%) was determined to be $149.4{\pm}15.6$ (common carp), $127.4{\pm}22.1$ (mud carp), $96.7{\pm}12.7$ (silver carp), $94.1{\pm}26.8$ (bighead carp) and $63.7{\pm}16.2$ (grass carp). The relative liver UCP2 expression of the five major Chinese carps, shows a close relationship with their food habit: benthos and detrituseating fish (common carp and mud carp) > planktivorious fish (silver carp and bighead carp) > herbivorious fish (grass carp). We suggest that liver UCP2 might be important for Chinese carps to detoxify cyanotoxins and bacteria in debris and plankton food.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.5
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• pp.740-744
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• 2002

The relationship of gonad weight and liver weight with body weight of six fish species viz; Catla catla, Labeo rohita, Cirrhina mrigala, Hypophthlamichthys molitrix, Ctenopharyngodon idella and Cyprinus carpio under the influence of artificial feed, broiler manure, buffalo manure, N:P:K (25:25:0) and a control pond were examined after a rearing period of one year. The positive relationship between gonad weight and body weight was significant which showed the dependence of gonadal development on body weight in all the six fish species. The currelation coefficients were higher in female fishes. However, the major carps had a much smaller proportional gonadal weight as compared with Chinese carps and a common carp due to their faster growth rate. The overall comparison of six fish species under different experimental treatments revealed that highest liver weight was observed for C. idella closely followed by C. carpio. The maximum correlation value was observed with H. molitrix under the broiler manure. The maximum Gonadosomatic Index (GSI) remained as 32.63 for C. carpio followed by C. idella. The maximum value for Hepatosomatic Index (HSI). remained 1.99 for C. idella followed by C. mrigala.

• Parasites, Hosts and Diseases
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• v.35 no.3
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• pp.211-214
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• 1997

Susceptibility of some vertebrates was examined to the early third-stage larvae (EL3) of Gnathostomn hispidum. The larvae collected from the Chinese loaches were infected to 4 silk carps, 3 snake heads, 3 bullfrogs. 5 mice and 9 albino rats. No worms were detected in fish. silk carps and snake heads. In 3 bullfrogs fed 30 larvae, a total of 9 EL3 was recovered in the gastrointestinal tract (8 larvae) and liver (ones). In 5 mice inferred with 50 larvae, a tolal of 37 (74.0%) advanced third-stage larvae (AdL3) was recovered from the muscle (31 larvae), liver (5 larvae) and kidney at 4 weeks after infection. In 9 albino rats iilfected with 115 larvae, a total of 40 (34.8%) AdLa was found in the muscle. The Inammalian hosts were found susceptible to the EL3 of G. hispinum from Chinese loaches.

• Korean Journal of Ichthyology
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• v.8 no.2
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• pp.68-73
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• 1996

This study was carried out to obtain the basic information on morphological and chromosomal charateristics in the three species of Chinese carps (grass carp; Ctenopharyngodon idellua, bighead carp; Aristichthys nobilis, and silver carp; Hypophthalmichthys molitrix) introduced to Korea from China. C. idellua was differ from A. nobilis and H. molitrix by the number of gill rakers, scales, fin rays, body proportion. A. nobilis and H. molitrix were similar in having ventral keel and many scale number, but H. molitrix was differ from A. nobilis by the connected gill rakers and body color pattern. Diploid chromosome and arm number (fundamental number, NF) of the three species were all the same to 2n=48 and NF=84. Diploid chromosome numbers in the three species are consisted of 10 pairs of metacentric chromosome, 8 pairs of submetacentric chromosome and 6 pairs of acro and/ or telocentric chromosome. Morphological and karyological relationship of the three Chinese carps are discussed.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.2
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• pp.196-200
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• 1998

The proximate composition of viscera of six fish species viz., Catla catla, Labeo, rohita Cirrhina mrigala, Hypophthalmichthys molitrix, Ctenopharyngodon idella and Cyprinus carpio under the influence of artificial feed $(T_1)$, broiler manure $(T_2)$, buffalo manure $(T_3)$, N:P:K (25:25:0 $T_4$) and control pond $(T_5)$ has been studied on final harvest after the end of experimental period of one year. The overall comparison of six fish species under five different treatments showed that Cyprinus carpio remained the best for visceral total protein contents. The maximum visceral protein content was recorded in fishes under $T_2$ except Cirrhina mrigala, while the minimum under $T_5$. The comparison of five treatments showed that maximum (6.69) percentage of fat in viscera of fish was recorded under $T_2$, closely followed by $T_4$ while minimum (4.89) under the effect of $T_5$. Among the six fish species under five treatments Cyprinus carpio showed maximum accumulation/deposition of total fats in viscera.

• BMB Reports
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• v.41 no.3
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• pp.204-209
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• 2008

The cDNAs encoding glutathione peroxidase (GPx) were cloned and sequenced from the liver of three Chinese carps with different tolerance to hepatotoxic microcystins, phyto-planktivorous silver carp (Hypophthalmichthys molitrix) and bighead carp (Aristichthys nobilis), and herbivorous grass carp (Ctenopharyngodon idellus). Using genome walker method, a 750 bp 5'-flanking region of the silver carp GPx gene was obtained, and several potential regulatory elements were identified in the promoter region of the GPx gene. The silver carp GPx gene was widely expressed in all tissues examined. Despite phylogenetic analysis, assigning this newly described carp GPx to the group of mammalian GPx2, the carp GPx seems more similar to GPx1 from a physiological point of view. The constitutive expression pattern of the three carp liver GPx gene, shows a positive relationship with their tolerance to microcystins.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.3 no.1
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• pp.19-26
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• 1970

During the summer of 1969, a series of experiments on the spawning and development of the eggs of grass carp and silver carp was performed with the following results, but the complete development of eggs Iras not accomplished: 1. The season of maturity for both species is estimated to be from the end of June to the beginning of August. 2. Pituitary glands for the use of inducing maturation should be obtained before the maturation season of its donor. 3. Silver carp as small as 40 cm in body length (48 cm in total length) with an age of 6 years were found to have reached maturity, and the number of eggs in the ovaries were from about 23 to 26 thousand for those with body lengths of 40-44.5 cm. 4. The conical-shaped net cloth incubator worked better than others, and each incubator was most effective when installed in parallel series to the water supply pipe with a valve for each incubator to control water flow. 5. During the egg development, if any cell or cells were displaced, the eggs eventually died. 6. The proper manipulation of brood fish before the egg-taking seems to be very Important, and close care should be paid to feeding them well and keeping them in a pond of proper water depth.

• Korean journal of applied entomology
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• v.38 no.2
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• pp.165-169
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• 1999

A study was performed on the susceptibility of Abate@ and Abate-S@ against 3rd instar larvae of 5 species of mosquitoes including Anopheles sinensis, Aedes dorsalis, Culex inatomii, Cx. pipiens pallens, Cx. tritaeniorhynchus and mosquito larvivorous fishes such as Chinese muddy loaches (Misgurnus muzolepis) and crucian carps (Carassius carassi~4s) in September, 1998. Cx. pipiens larvae showed that the LC5os of both Abatea and Abate-Sa were 0.006 ppm. The LC95 values of this mosquito species appeared 0.070 pprn of Abate@ and 0.035 pprn of Abate-S@ which were more susceptible than the dosage (1.0 ppm) recommended from WHO. An. sinensis larvae appeared 0.009 pprn of LC50 and 0.025 pprn of LCgs. The larvae of Cx. inatomii and Ae. dorsalis showed the most highly susceptible to Abate-S@ among the 5 mosquito species with LC50 values of 1.9 x pprn and 1.1 x pprn respectively, and the LC95 values were 5.2 x 10-hppm and 1.4 x ppm, respectively. On the other hand, Cx. tritaeniorhynchus appeared least affected by Abate-Sa. and was brought 0.048 pprn of LC50 and 0.808 pprn of LCg5. These two insecticides were much less toxic to the fishes than mosquito larvae showing the LC, value of Chinese muddy loaches at 24.145 pprn of Abate and 10.750ppm of Abate-S@. This fish species showed that the LCsos of Abate@ and Abate-S@ were 27.567 pprn and 14.775 ppm, respectively. In case of crucian carps, their LC, values were 7.914 pprn of Abate@ and 6.480 pprn of Abate-S@. Therefore, the maximum safe dosages of the insecticides to the fishes were 8 times as high as the values of LCg5 of the mosquito larvae. This study suggests that Abate@ and Abate-S@ demonstrated highly potential insecticides for further larger scale operational integrated control in some proper aquatic places if other aquatic invertebrates are also safe to the insecticides.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.4 no.3_4
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• pp.113-120
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• 1971

In 1971, about 150,000 fingerlings of grass and silver carps were produced and distributed from the Fishculture Laboratory of Pusan fisheries College. The adults Were those transplanted from Japan in 1963. Findings during the process of this production are summarized as following : 1. Brood fish must be handled with a great care during the catching, hormone injection and maturity inspection so as the fish are not injured, otherwise, the eggs would not mature perfectly. Scale fall also affects significantly. 2. The amount of pituitary to be injected is preferable to be 2 to 3 times or more in donor's body weight to obtain good results. 3. The eggs should be spawned or stripped and inseminated as soon as mature. If the spawning is delayed the eggs become overmature and the hatching rate decreases significantly. 4. The water once used for the incubation of eggs should not be reused. The eggs under hatching process were melted away when water once used for incubation was reused. 5. A great care must be paid to keep water in quality when the net cage culture system is employed for raising fry or early fingerlings of Chinese carps. The best method to keep water in quality is to supply water in the cage continuously through a pipe or hose. 6. Heavy outbreaks of Spirogyra occured when clear well water was supplied into the cage, and a great number of fry or fingerlings were trapped or entangled resulting in a significant decrease of fish under rearing. It was prevented when moderately bloom-ed pond water was supplied into the cage. 7. Silver carp fry are usually captured and transported in the cool season because they can not be handled in the warm season owing to a high mortality, but cage cultured small size fingerlings ranging from 1.5 to 2 cm in total length are easily handled and transported even in the hot summer season, thus it enables the fish farmers to start growing them one season earlier.