• Asian-Australasian Journal of Animal Sciences
• /
• 제21권12호
• /
• pp.1815-1820
• /
• 2008

The aim of this study was to extract chicken leg bone, which is a by-product of industrial poultry processing, using different heating temperatures (80, 90 and $100^{\circ}C$) and durations (5, 10 and 15 min). The pH value, soluble protein content, peptide content and antioxidative properties, including superoxide anion scavenging ability, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging ability, reducing capacity and inhibitory activity of linoleic acid peroxidation, were measured. The results showed no significant differences (p>0.05) in pH value among all treatments. Decreased soluble protein content and peptide content were observed in chicken leg bone extract obtained under higher heating temperatures (90 or $100^{\circ}C$) and longer heating durations (10 or 15 min). In antioxidative properties, the extracts which were heated at 90 or $100^{\circ}C$ for 15 min exhibited significantly higher superoxide anion scavenging ability, DPPH free radical scavenging ability, reducing capacity and inhibitory activity of linoleic acid peroxidation (p<0.05).

• Asian-Australasian Journal of Animal Sciences
• /
• 제21권5호
• /
• pp.732-737
• /
• 2008

This study developed a natural ingredient as a functional food possessing properties of attenuation of hypertension and cardiovascular hypertrophy. In a previous study hydrolysates obtained from chicken leg bone protein using Alcalase strongly inhibited angiotensin I converting enzyme (ACE) in vitro. In particular, hydrolysate (A4H) from four hours of incubation exhibited the highest ACE inhibitory activity (IC50 = 0.545 mg/ml). A4H was selected as a potent ACE inhibitor and orally administrated to spontaneously hypertensive rats (SHR) for eight weeks to investigate attenuating effects on age-related development of hypertension and cardiovascular hypertrophy. Results showed that treatment with A4H of SHRs attenuated the development of hypertension as effectively as the clinical antihypertensive drug captopril. Moreover, a significantly lower heart to body weight ratio and thinness of coronary arterial wall was observed in SHRs that had been treated with A4H or captopril. The results suggest that A4H can be utilized in developing an ACE inhibitor as a potential ingredient of functional foods to alleviate hypertension and cardiovascular hypertrophy.

• 한국식품영양과학회지
• /
• 제32권2호
• /
• pp.307-310
• /
• 2003

소족편, 소족에 닭을 첨가한 족편 및 건대구를 첨가한 족편의 영양성분을 검토한 결과 수분함량은 시료족편간에 유사하였고, 조단백질 함량은 소족편이 닭이나 건대구 첨가 족편보다 약간 높았으며 조지방과 회분함량은 낮았다. 불포화지방산의 비율은 건대구 첨가 족편이 가장 높았으나, linoleic acid는 닭첨가 족편 중에 가장 높은 비율로 함유되어 있었다. 아미노산조성은 g1ycine, proline, alanine, glutantc acid 순으로 높았으나, glycine과 alanine은 건대구 첨가 족편에, glutamic acid는 소족편에 가장 많이 함유되어 있었다. 필수아미노산중에서 Iysine이 유사하게 모든 족편에서 가장 많았으며 methionine은 닭 첨가 족편에, valine은 소족편에 가장 많이 함유되어 있었다. 3종 족편중 건대구 첨가족편은 다른 족편보다 필수아미노산의 조성비가 가장 낮았다. 칼슘과 인은 닭 첨가 족편에 가장 많았고 건대구 첨가 족편에 가장 적었다. 이상의 결과로부터 족편은 사태나 사골의 용출액보다 조단백질과 칼슘함량이 2~3배 높은 양 농축되어 있어 영양적으로 우수한 음식임을 알 수 있었다 따라서, 족편을 조리하기 간편하며 현대인들의 구미에 맞는 상품으로 개발하여 우리 고유의 전통음식을 지켜 나가도록 노력해야 하겠다.

• Journal of Animal Science and Technology
• /
• 제65권2호
• /
• pp.336-350
• /
• 2023

Two experiments were conducted in this research. Experiment 1 investigated the spatial expression characteristics of calcium (Ca) and phosphorus (P) transporters in the duodenum, jejunum, and ileum of 21-day-old broilers provided with adequate nutrient feed. Experiment 2 evaluated the effects of dietary vitamin D₃ (VD₃) concentration (0, 125, 250, 500, 1,000, and 2,000 IU/kg) on growth performance, bone development, and gene expression levels of intestinal Ca and P transporters in 1-21-day-old broilers provided with the negative control diet without supplemental VD₃. Results in experiment 1 showed that the mRNA levels of calcium-binding protein 28-kDa (CaBP-D28k), sodium-calcium exchanger 1 (NCX1), plasma membrane calcium ATPase 1b (PMCA1b), and IIb sodium-phosphate cotransporter (NaPi-IIb) were the highest in the broiler duodenum. By contrast, the mRNA levels of inorganic phosphate transporter 1 (PiT-1) and 2 (PiT-2) were the highest in the ileum. Results in experiment 2 showed that adding 125 IU/kg VD₃ increased body weight gain (BWG), feed intake (FI), bone weight, and percentage and weight of Ca and P in the tibia and femur of 1-21-day-old broilers compared with the negative control diet (p < 0.05). The rise in dietary VD₃ levels from 125 to 1,000 IU/kg further increased the BWG, FI, and weights of the bone, ash, Ca, and P (p < 0.05). No difference in growth rate and leg bone quality was noted in the broilers provided with 1,000 and 2,000 IU/kg VD₃ (p > 0.05). Supplementation with 125-2,000 IU/kg VD₃ increased the mRNA abundances of intestinal Ca and P transporters to varying degrees. The mRNA level of CaBP-D28k increased by 536, 1,161, and 28 folds in the duodenum, jejunum, and ileum, respectively, after adding 1,000 IU/kg VD₃. The mRNA levels of other Ca and P transporters (PMCA1b, NCX1, NaPi-IIb, PiT-1, and PiT-2) increased by 0.57-1.74 folds by adding 1,000-2,000 IU/kg VD₃. These data suggest that intestinal Ca and P transporters are mainly expressed in the duodenum of broilers. Moreover, the addition of VD₃ stimulates the two mineral transporter transcription in broiler intestines.