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http://dx.doi.org/10.5713/ajas.2008.70599

Attenuating Development of Cardiovascular Hypertrophy with Hydrolysate of Chicken Leg Bone Protein in Spontaneously Hypertensive Rats  

Cheng, Fu-Yuan (Department of Animal Science, National Chung-Hsing University)
Wan, Tien-Chun (Department of Animal Science, National Chung-Hsing University)
Liu, Yu-Tse (Department of Animal Science, National Chung-Hsing University)
Lai, Kung-Ming (Department of Health Diet and Restaurant Management, Chung Shan Medical University)
Lin, Liang-Chuan (Department of Animal Science, National Chung-Hsing University)
Sakata, Ryoichi (School of Veterinary Medicine, Azabu University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.21, no.5, 2008 , pp. 732-737 More about this Journal
Abstract
This study developed a natural ingredient as a functional food possessing properties of attenuation of hypertension and cardiovascular hypertrophy. In a previous study hydrolysates obtained from chicken leg bone protein using Alcalase strongly inhibited angiotensin I converting enzyme (ACE) in vitro. In particular, hydrolysate (A4H) from four hours of incubation exhibited the highest ACE inhibitory activity (IC50 = 0.545 mg/ml). A4H was selected as a potent ACE inhibitor and orally administrated to spontaneously hypertensive rats (SHR) for eight weeks to investigate attenuating effects on age-related development of hypertension and cardiovascular hypertrophy. Results showed that treatment with A4H of SHRs attenuated the development of hypertension as effectively as the clinical antihypertensive drug captopril. Moreover, a significantly lower heart to body weight ratio and thinness of coronary arterial wall was observed in SHRs that had been treated with A4H or captopril. The results suggest that A4H can be utilized in developing an ACE inhibitor as a potential ingredient of functional foods to alleviate hypertension and cardiovascular hypertrophy.
Keywords
Angiotensin I Converting Enzyme (ACE); Chicken Leg Bone Protein; Hydrolysate; Antihypertensive Effect; Cardiovascular Hypertrophy;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
Times Cited By Web Of Science : 1  (Related Records In Web of Science)
Times Cited By SCOPUS : 2
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1 Ishimitsu, T., T. Honda, S. Ohta, A. Akashiba, T. Takahashi, T. Kameda, M. Yoshii, J. Minami, M. Takahashi, H. Ono and H. Matsuoka. 2006. Cardiorenal protective effects of yearlong antihypertensive therapy with a angiotensin-converting enzyme inhibitor or a calcium channel blocker in spontaneously hypertensive rat. Am. J. Hyperten. 19:1233-1240.   DOI   ScienceOn
2 Muguruma, M., K. Katayama, H. Fuchu and M. Sugiyama. 2003. Peptic hydrolysate of porcine crude myosin has many active fractions inhibiting angiotensin I-converting enzyme. Asian-Aust. J. Anim. Sci. 16:1384-1389.   과학기술학회마을   DOI
3 Ikeda, Y., T. Nakamura, H. Takano, H. Kimura, J. E. Obata, S. Takeda, A. Hata, K. Shido, S. Mochizuki and Y. Yoshida. 2000. Angiotensin II-induced cardiomyocyte hypertrophy and cardiac fibrosis in stroke-prone spontaneously hypertensive rats. J. Lab. Clin. Med. 135:353-359.   DOI   ScienceOn
4 Miguel, M., I. Recio, J. A. Gomez-Ruiz, M. Ramos and R. Lopez-Fandino. 2004. Angiotensin I-converting enzyme inhibitory activity of peptides derived from egg white proteins by enzymatic hydrolysis. J. Food Prot. 67:1914-1920.   DOI
5 Moalic, J. M., J. Bercovici and B. Swynghedauw. 1984. Moysin heavy chain and actin fractional rates of synthesis in normal and overload rat heart ventricles. J. Mol. Cell Cardiol. 16:875-884.   DOI   ScienceOn
6 Dhlof, B., K. Pennert and L. Hansson. 1992. Reversal of left ventricular hypertrophy in hypertensive patients. Am. J. Hypertens. 5:95-110.   DOI
7 Diez, J. and C. Laviades. 1997. Monitoring fibrillar collagen turnover in hypertensive heart disease. Cardiovasc. Res. 35: 202-205.   DOI   ScienceOn
8 Freslon, J. L. and J. F. Giudicelli. 1983. Compared myocardial and vascular effects of captopril and dihydralazine during hypertension development in spontaneously hypertensive rats. Br. J. Pharmacol. 80:533-543.   DOI   ScienceOn
9 Habib, G. 1997. Reappraisal of the importance of heart rate as a risk factor for cardiovascular morbidity and mortality. Clin. Ther. 19:39-52.
10 Hu, W. Y., Y. J. Han, L. Z. Gu, M. Piano and P. de Lanerolle. 2007. Involvement of ras-regulated myosin light chain phosphorylation in the captopril effects in spontaneously hypertensive rats. Am. J. Hyperten. 20:53-61.   DOI   ScienceOn
11 Perski, A., A. Hamsten, K. Lindvall and T. Theorell. 1988. Heart rate correlates with severity of coronary atherosclerosis in young postinfarction patients. Am. Heart J. 116:1369-1373.   DOI   ScienceOn
12 Ondetti, M. A., N. J. Williams, E. F. Sabo, J. Pluvec, E. R. Weaver and O. Kocy. 1971. Angiotensin-converting enzyme inhibitors from the venom of Bothrops jararaca: isolation, elucidation of structure and synthesis. Biochem. 10:4033-4042.   DOI   ScienceOn
13 Kannel, W. B. 1996. Blood pressure as a cardiovascular risk factor. J. Am. Med. Assoc. 275:1571-1576.   DOI   ScienceOn
14 Katayama, K., H. Fuchu, A. Sakata, S. Kawahara, K. Yamauchi, Y. Kawamura and M. Muguruma. 2003. Angiotensin I-converting enzyme inhibitory activities of porcine skeletal muscle proteins following enzyme digestion. Asian-Aust. J. Anim. Sci. 16:417-424.   과학기술학회마을   DOI
15 Jang, A. and M. Lee. 2005. Purification and identification of angiotensin converting enzyme inhibitory peptides from beef hydrolysates. Meat Sci. 69:653-661.   DOI   ScienceOn
16 Johnston, C. I., L. M. Burrell, R. Perich, K. Jandeleit and B. Jackson. 1992. The tissue renin-angiotensin system and its functional role. Clin. Exp. Pharmacol. Physiol. 19:1-5.
17 Jung, W. K., E. Mendis, J. Y. Je, P. J. Park, B. W. Son, H. C. Kim, Y. K. Choi and S. K. Kim. 2006. Angiotensin I-converting enzyme inhibitory peptide from yellowfin sole (Limanda aspera) frame protein and its antihypertensive effect in spontaneously hypertensive rats. Food Chem. 94:26-32.   DOI   ScienceOn
18 Vercruysse, L., J. V. Camp and G. Smagghe. 2005. ACE inhibitory peptides derived from enzymatic hydrolysates of animal muscle protein: A review. J. Agric. Food Chem. 53:8106-8115.   DOI   ScienceOn
19 Ondetti, M. A., B. Rubin and D. W. Cushman. 1977. Design of specific inhibition of angiotensin-converting enzyme: new class of orally active antihypertensive agents. Sci. 196:441-444.   DOI
20 Materson, B. J., D. J. Reda and D. W. Williams. 1998. Comparison of effects of antihypertensive drugs on heart rate: changes from baseline by baseline group and over time. Am. J. Hypertens. 11:597-601.   DOI   ScienceOn
21 Materson, B. J., D. J. Reda and D. W. Williams. 1999. Effects of antihypertensive single-drug therapy on heart rate. Am. J. Hypertens. 12: 9-11.   DOI   ScienceOn
22 Laragh, J. H., L. Bear, H. R. Brunner, F. G. Buhler, J. E. Ealey and E. D. Vaughan. 1972. Renin, angiotensin and aldosterone system in pathogenesis and management of hypertensive vascular disease. Am. J. Med. 52:633-652.   DOI   ScienceOn
23 Saiga, A., T. Okumura, T. Makihara, S. Katsuta, T. Shimizu, R. Yamada and T. Nishimura. 2003. Angiotensin I-Converting enzyme inhibitory peptides in a hydrolyzed chicken breast muscle extract. J. Agric. Food Chem. 51:1741-1745.   DOI   ScienceOn
24 Sipola, M., P. Finckenberg, J. Santisteban, R. Korpela, H. Vapaatalo and M. L. Nurminen. 2001. Long-term intake of milk peptides attenuates development of hypertension in spontaneously hypertensive rats. J. Physiol. Pharmacol. 52: 745-754.
25 Chen, S. C., J. Z. Su, K. Wu, W. Y. Hu, D. G. Gardner and D. G. Chen. 1998. Early captopril treatment prevents hypertrophy dependent gene expression in hearts of SHR. Am. J. Physiol. 274:1511-1517.   DOI
26 Li, G., G. Le, Y. Shi and S. Shrestha. 2004. Angiotensin I-converting enzyme inhibitory peptides derived from food proteins and their physiological and pharmacological effects. Nutr. Res. 24:469-486.   DOI
27 Tsutsui, H., Y. Ishibashi, M. Takahashi, T. Namba, H. Tagawa, K. I. Yoshida and A. Takeshita. 1999. Chronic colchicine administration attenuates cardiac hypertrophy in spontaneously hypertensive rats. J. Mol. Cell Cardiol. 31:1203-1213.   DOI   ScienceOn
28 Brian, J. and R. Rosario. 2005. Hypertension: a review and rationale of treatment. Disease-a-Month 51:548-614.   DOI   ScienceOn
29 Wu, J. and X. Ding. 2001. Hypotensive and physiological effect of angiotensin converting enzyme inhibitory peptides derived from soy protein on spontaneously hypertensive rats. J. Agric. Food Chem. 49:501-506.   DOI   ScienceOn
30 Miguel, M., R. Lopez-Fandino, M. Ramos and A. Aleixandre. 2005. Short-term effect of egg white hydrolysate products on arterial blood pressure of hypertensive rats. Br. J. Nutr. 94: 731-737.   DOI   ScienceOn
31 Miguel, M., R. Lopez-Fandino, M. Ramos and A. Aleixandre. 2006. Long-term intake of egg white hydrolysate attenuates the development of hypertension in spontaneously hypertensive rats. Life Sci. 78:2960-2966.   DOI   ScienceOn
32 Yang, H. Y., S. C. Yang, J. R. Chen, Y. H. Tzeng and B. C. Han. 2004. Soybean protein hydrolysate prevents the development of hypertension in spontaneously hypertensive rats. Br. J. Nutr. 92:507-512.   DOI   ScienceOn
33 Cheng, F. Y., Y. T. Liu, T. C. Wan, C. M. Chen and L. C. Lin. 2007b. The study on development of bioactive peptides derived from chicken bone protein II: antioxidative activity. Taiwan J. Agric. Chem. Food Sci. 45:84-90.
34 Lee, K. J., S. B. Kim, J. S. Ryu, H. S. Shin and J. W. Lim. 2005. Separation and purification of angiotensin converting enzyme inhibitory peptides derived from goat`s milk casein hydrolysates. Asian-Aust. J. Anim. Sci. 18:741-746.   과학기술학회마을   DOI
35 Turner, A. J. and N. M. Hooper. 2002. The angiotensin-converting enzyme gene family: genomics and pharmacology. Trends Pharmacol. Sci. 23:177-183.   DOI   ScienceOn
36 Cheng, F. Y., Y. T. Liu, T. C. Wan, K. M. Lai and L. C. Lin. 2007a. The study on development of bioactive peptides derived from chicken bone protein I: preparation of chicken leg bone hydrolysates and ACE inhibitory activity. Taiwan J. Agric. Chem. Food Sci. 45:21-26.
37 Bhoola, K. D., C. D. Figueroa and K. Worthy. 1992. Bioregulation of kinins - kallikreins, kininogens, and kininases. Pharmacol. Rev. 44:1-80.
38 Cheng, F. Y., Y. T. Liu, T. C. Wan, L. C. Lin and R. Sakata. 2008. The development of angiotensin I converting enzyme inhibitor derived from chicken bone protein. Anim. Sci. J. 79:121-127.
39 Cohen, M. L. 1985. Synthetic and fermentation-derived angiotensin converting enzyme inhibitors. Annu. Rev. Pharmacol. Toxicol. 25:307-323.   DOI   ScienceOn