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Enhanced Biomass and ${\gamma}$-Linolenic Acid Production of Mutant Strain Arthrospira platensis

  • Choi, Gang-Guk;Bae, Myong-Sook;Ahn, Chi-Yong;Oh, Hee-Mock
    • Journal of Microbiology and Biotechnology
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    • v.18 no.3
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    • pp.539-544
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    • 2008
  • A mutant of Arthrospira platensis PCC 9108, strain M9108, obtained by mutagenesis with UV treatment, was able to mixotrophically grow in an SOT medium containing 40 g of glucose/l. The biomass and specific growth rate of strain M9108 (4.10 g/l and 0.70/d) were 1.9-fold and 1.4-fold higher, respectively, than those of the wild type (2.21 g/l and 0.58/d) under mixotrophic culture condition. In addition, when compared with the wild type, the content of ${\gamma}$-linolenic acid (GLA) in the mutant was increased when glucose concentration was increased. Compared with the wild type, the GLA content of the mutant was 2-fold higher in autotrophic culture and about 3-fold higher in mixotrophic culture. Thus, the mutant appears to possess more efficient facility to assimilate and metabolize glucose and to produce more GLA than its wild-type strain.

Association between obesity and high-sensitivity C-reactive protein in Korean adults without cardiovascular disease

  • Heashoon, Lee
    • Journal of Korean Biological Nursing Science
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    • v.25 no.1
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    • pp.32-42
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    • 2023
  • Purpose: This study aimed to investigate the association between obesity and high-sensitivity C-reactive protein (hs-CRP) levels in Korean adults without cardiovascular disease (CVD). Methods: The subjects were 3,634 adults, and data were extracted from the seventh Korea National Health and Nutrition Examination Survey (KNHANES VII-3). A complex sampling design analysis was applied to reflect the stratified and clustered weights. The data were analyzed using the complex sample Rao-Scott chi-square test and multiple logistic regression analysis (in SPSS for Windows version 26.0). Obesity, according to body mass index (BMI), was defined as obesity (BMI = 25-29.9 kg/m2), high obesity (BMI = 30-34.9 kg/m2), and super-high obesity (BMI ≥ 35 kg/m2), and abdominal obesity (AO) was defined as a waist circumference (WC) ≥ 90 cm in males and WC ≥ 85 cm in females. Results: The odds ratios for moderate CVD risk (hs-CRP; 1-3 mg/dL) were 2.21, 4.16, and 7.13 in the obesity, high obesity, and super-high obesity groups, respectively, compared to the normal BMI group. The odds ratio for moderate CVD risk was 2.18 in males with AO and 1.88 in females with AO. The odds ratios for high CVD risk (hs-CRP > 3 mg/L) were 4.40 and 17.55 in the high obesity and super-high obesity groups, respectively, compared to the normal BMI group. Conclusion: This study provides evidence that early detection and prevention programs for CVD should include obesity-related interventions aiming to modulate hs-CRP.

Physicochemical Properties of Isolated Peptides from Hwangtae (yellowish dried pollack) Protein Hydrolysate

  • Cho, San-Soon;Lee, Hyo-Ku;Han, Chi-Won;Seong, Eun-Soo;Yu, Chang-Yeon;Kim, Myong-Jo;Kim, Na-Young;Kang, Wie-Soo;Ko, Sang-Hoon;Son, Eun-Hwa;Choung, Myoung-Gun;Lim, Jung-Dae
    • Preventive Nutrition and Food Science
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    • v.13 no.3
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    • pp.204-211
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    • 2008
  • Fish protein hydrolysates (FPHs) with different degrees of hydrolysis by treatment with alcalase, pronase, flavourzyme and trypsin and isolated peptide were prepared from Hwangtae (yellow dried pollack, Theragra chalcogramma). Hwangtae protein hydrolysate was fractionated according to the molecular weight into six major types of APO1 (1.3 kDa), APO2 (1 kDa), APO3 (<1 kDa), APACE (<1 kDa), APG1 (70 kDa) and APG2 (70 kDa) isolated from the hydrolysate using consecutive chromatographic methods. Soluble peptide were produced from Hwangtae and evaluated for their nutritional and functional properties. Some functional properties of FPHs were assessed and compared with those of egg albumin or the soybean protein. APO2 had the highest nitrogen solubility value (94.2%), emulsion capacity and emulsion stability of the Alaska Pollack peptide ranged from 12.4 to 39.5 (mL of oil per 200 mg of protein) and 44.0% to 77.5%, respectively. Highest and lowest fat adsorption values were observed for APG1 (9.9 mL of oil per gram of protein) and APO3 (3.8 mL of oil per gram of protein), respectively.

Changes of Self-efficacy, Depression, and Posttraumatic Growth in Survivors with Breast Cancer Participating Breast Cancer Prevention Volunteering (유방암예방 자원봉사활동에 참여한 유방암 생존자들의 자기효능감, 우울 및 외상 후 성장의 변화)

  • Yi, Myungsun;Cha, Jieun;Ryu, Youngmi
    • The Journal of the Korea Contents Association
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    • v.14 no.4
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    • pp.256-266
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    • 2014
  • Purpose : This study was to explore the long-term effects of breast cancer prevention volunteering on self-efficacy, depression, and posttraumatic growth among survivors with breast cancer. Methods: This pilot study utilized a longitudinal design. Participants were 14 women with breast cancer who participated in the breast cancer prevention educational program The data were collated between February and August in 2013. Self-report questionnaires were used to measure the self-efficacy for self-management of breast cancer, depression, and posttraumatic growth at pre-education(T0), 1 month(T1), 3 months(T2), and 6 months(T1) post-education. A generalized estimating equation regression model was used to identify the effects. Results: The scores of self-efficacy increased significantly at T1(${\chi}^2$=5.56, p=.0l8) and D(${\chi}^2$=4.56, p=.033) compared with T0. Depression and posttraumatic growth remained stable with fairly good levels throughout the 6-month period. Conclusion: The results of this study suggest that breast cancer prevention volunteering in survivors with breast cancer can be effective for enhancing their self-efficacy and maintaining psychological well-bang. Also the study showed that such volunteering activity could be a useful program not only for the general public but also for breast cancer survivors.

Trend of Influencing Factors on Health-related Quality of Life in Korean Elderly (한국 노인의 건강관련 삶의 질과 영향요인 추이)

  • An, Jisook;Choi, Hye Young
    • Journal of Korean Public Health Nursing
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    • v.32 no.2
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    • pp.275-287
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    • 2018
  • Purpose: This study examined the trends of socio-demographic and health factors affecting the health-related quality of life (HRQoL) in Korean elderly. Methods: Data were obtained from the Fourth, Fifth and Sixth Korean National Health and Nutrition Examination Survey (KNHANES V-1, 2007, KNHANES V-1, 2010, KNHANES V-2, 2014). The sample consisted of 3,668 Korean individuals aged 65 years and older. The data were analyzed using a t-test, Chi-square test and multiple linear regression using the SPSS/WIN 20.0 program. Results: The HRQoL is improving consistently and the affecting factors on the HRQoL were the education level, activity limitation, subjective health and walking days per week in the three data of 2007, 2010, and 2014. Conclusion: A health care program focusing on improving activity limitation and physical activity should be considered for the improvement of HRQoL for the elderly in the community.

Abducens Nerve Palsy after Lumbar Spinal Fusion Surgery with Inadvertent Dural Tearing

  • Cho, Dae-Chul;Jung, Eul-Soo;Chi, Yong-Chul
    • Journal of Korean Neurosurgical Society
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    • v.46 no.6
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    • pp.581-583
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    • 2009
  • Abducens nerve palsy associated with spinal surgery is extremely rare. We report an extremely rare case of abducens nerve palsy after lumbar spinal fusion surgery with inadvertent dural tearing, which resolved spontaneously and completely. A 61-year-old previous healthy man presented with chronic lower back pain of 6 weeks duration and 2 weeks history of bilateral leg pain. He was diagnosed as having isthmic spondylolisthesis at L4-5 and L5-S1, and posterior lumbar interbody fusion was conducted on L4-5 and L5-S1. During the operation, inadvertent dural tearing occurred, which was repaired with a watertight dural closure. The patient recovered uneventfully from general anesthesia and his visual analogue pain scores decreased from 9 pre-op to 3 immediately after his operation. However, on day 2 he developed headache and nausea, which were severe when he was upright, but alleviated when supine. This led us to consider the possibility of cerebrospinal fluid leakage, and thus, he was restricted to bed. After an interval of bed rest, the severe headache disappeared, but four days after surgery he experienced diplopia during right gaze, which was caused by right-side palsy of the abducens nerve. Under conservative treatment, the diplopia gradually disappeared and was completely resolved at 5 weeks post-op.

Formation and Related-Behavior of Micro-bowl Morphology Consisting of Ionic Palladium(II) Complexes

  • Kim, Cho-Rong;Kim, Chi-Won;Noh, Tae-Hwan;Lee, Young-A;Hong, Jong-Ki;Jung, Ok-Sang
    • Bulletin of the Korean Chemical Society
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    • v.31 no.8
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    • pp.2223-2227
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    • 2010
  • Reaction of [(bpy)Pd]$(PF_6)_2$ (bpy = 2,2'-bipyridine) with racemic bis(isonicotinoyl)-1,1'-bi-2-naphtholate (L) in acetone, and followed by addition of chloroform and solvent evaporation allows to form amorphous micro-bowl morphology consisting of $[(bpy)PdL]_2(PF_6)_4$ without any template or additive. In contrast, the reaction and recrystallization in acetone for 1 week produce parallel-piped single crystals consisting of $[(bpy)_3Pd_3({\mu}_3-HPO_4)_2](PF_6)_2$. The formations of micro-bowl and parallel-piped single crystal morphologies appear to be primarily associated with the kinetic and thermodynamic control, respectively. The formation of micro-bowls may be attributed to eruption of organic solvents. Cosolvent effects and chemical properties on the formation of micro-bowl morphology have been observed.

Interface and Surface Properties by Surface Treatment of Zirconia for All Ceramic Crown (전부도재관용 지르코니아의 표면처리에 따른 표면특성 및 계면특성 관찰)

  • Kim, Chi-Young;Chung, In-Sung;Choi, Sung-Min
    • Journal of Technologic Dentistry
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    • v.35 no.2
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    • pp.137-142
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    • 2013
  • Purpose: This study was to observe the surface and interfacial characteristic of Zirconia by surface treatment. And it was observed the roughness and contact angle according to processing, and the interfacial properties by surface treatment on zirconia. Methods: The oxide formation and ion diffusion between core and veneer ceramic were determined by the X-ray Dot Mapping of EPMA(Electron probe micro analyzer). The roughness was measured by 3D Digital microscope and the contact angle according to processing of zirconia was observed using distilled water on the surface. Results: The surface roughness of the specimens Z04, Z12, Z15 was measured $0.67({\pm}0.03){\mu}m$, $0.50({\pm}0.12){\mu}m$, $0.35({\pm}0.09){\mu}m$, respectively. As results of contact angle test, Z04, Z12, Z15 of specimen group without binder treatment was measured $46.79({\pm}3.17)^{\circ}$, $57.47({\pm}4.83)^{\circ}$, $56.19({\pm}2.66)^{\circ}$, respectively. but, L04, L12, L15 of specimen group without binder treatment was measured $63.84({\pm}2.20)^{\circ}$, $66.08({\pm}0.16)^{\circ}$, $65.10({\pm}1.01)^{\circ}$, respectively. Average contact angle of L15 was measured $65.10({\pm}1.01)^{\circ}$. In X-ray Dot Mapping results, thickness of binder including Al element was measured that each of L04, L12, L15 were $20{\mu}m$, $15{\mu}m$, $10{\mu}m$. Conclusion: The more rough surface increases the wettability, but the sintered exclusive binder decreases the wettability.

Ethanol Pool Fire Extinguishing Experiment Using Twin-fluid Nozzle Supplied with Water and Air (물과 공기가 공급되는 2유체노즐을 활용한 에탄올 풀화재 소화 실험)

  • Jeong, Chan Seok;Lee, Chi Young
    • Fire Science and Engineering
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    • v.33 no.3
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    • pp.37-43
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    • 2019
  • In this study, ethanol pool fire extinguishing experiments were conducted using a twin-fluid nozzle. Ethanol pool fires, 5.027×10-3 ㎡ and 1.131×10-2 ㎡ in size (80 mm and 120 mm in fuel pan diameter, respectively), were tested, and the flow rates supplied to the twin-fluid nozzle for fire extinguishing were 156-483 g/min and 20-70 L/min for water and air, respectively. The heat release rate increased with increasing fire source area, and heat release rates of 5.027×10-3 ㎡ and 1.131×10-2 ㎡ in size were measured to be 1.01 kW and 5.51 kW, respectively. For both fire source cases in the present experimental range, regardless of the water flow rates, the ethanol fires were extinguished successfully under the high air flow rate condition (e.g., above 40 L/min). On the other hand, under all water flow rate conditions, the fire extinguishing time and water consumption decreased with increasing air flow rate, which were approximately 23 s and 185 g under high air flow rate conditions (e.g., above 50 L/min), respectively. Based on the water consumption per heat release rate, the present experimental data were compared with the previous ones using a single-fluid nozzle, and it was found that the twin-fluid nozzle could extinguish a fire with a lower water consumption than a single-fluid one.

Population density of chigger mites, the vector of tsutsugamushi disease in Chollanam-do, Korea (전라남도에서 쯔쯔가무시병 매개체인 털진드기의 개체군 밀도에 관한 조사)

  • 송현제;김개환
    • Parasites, Hosts and Diseases
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    • v.34 no.1
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    • pp.27-34
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    • 1996
  • The geographical distribution and population density of rodents and chigger mites at six localities of Chollanam-do were investigated from October to December in 1993. Among total 142 field rodents collected by the modified Chemin wooden traps, 131 rodents were Aponemw usrari,us (92.3%) and 11 were Crocidurc losiurc (7.7%). Out of 142 field rodents, 92 were parasitized by chiggers, showing 69.0% of the infestation rate and 74.2 of the chiggor index. Infestation rate and chigger index of A. omfernrius and C. lusiurc were 73.3%, 80.4 and 18.2%, 0.5, respectively. From the trapped field rodents, 10,532 chiggers were collected and identified with 11 species of 4 genera. Leptotrombidium pcLLinun. the vector 3pecies of tsutsugamushi disease, was the dominant species, showing 8,038 chi99ers (76.31%). L. scutellnre was the second dominant species showing 1,359 chiggers (12.9%). The distribution of chigger mites was clearly localized by the species, showing the different dominant species according to localities. The predominant species was 1. scutellare (100%) in Changhung-gun, 1. scutellcre (41.5%) in Posong-gun, 1. pnllidum (88.8%) in Hwasun- gun, 1. pnLlidunl (59.2%) in Koksong-gun, 1. zetum (77.3%) in Hampyong-gun, and 1. pclpcle (63.4%) in Tamyang-gun. Regarding to the geographical distribution of chigger population density, the infestation rate and chigger index was most high in Hwasun-gun as 62.4% and 216.2 respectively, and next high in Koksong-gun as 22.4% and 77.7%, respectively.

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