Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Enhanced Biomass and ${\gamma}$-Linolenic Acid Production of Mutant Strain Arthrospira platensis

  • Choi, Gang-Guk (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Bae, Myong-Sook (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Ahn, Chi-Yong (Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB)) ;
  • Oh, Hee-Mock (Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology(KRIBB))
  • Published : 2008.03.31
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Abstract

A mutant of Arthrospira platensis PCC 9108, strain M9108, obtained by mutagenesis with UV treatment, was able to mixotrophically grow in an SOT medium containing 40 g of glucose/l. The biomass and specific growth rate of strain M9108 (4.10 g/l and 0.70/d) were 1.9-fold and 1.4-fold higher, respectively, than those of the wild type (2.21 g/l and 0.58/d) under mixotrophic culture condition. In addition, when compared with the wild type, the content of ${\gamma}$-linolenic acid (GLA) in the mutant was increased when glucose concentration was increased. Compared with the wild type, the GLA content of the mutant was 2-fold higher in autotrophic culture and about 3-fold higher in mixotrophic culture. Thus, the mutant appears to possess more efficient facility to assimilate and metabolize glucose and to produce more GLA than its wild-type strain.

Keywords

References

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