• Journal of Biosystems Engineering
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• 제41권4호
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• pp.365-372
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• 2016

Purpose: Tea is the most frequently consumed drink worldwide, next to water. About 75% of the total world tea production includes black tea, and withering is one of the major processing steps critical for the quality of black tea. There are two types of tea withering methods: physical and chemical withering. Withering can be achieved by using tat, tunnel, drum, and trough withering systems. Of these, the trough withering system is the most commonly used. This study focuses on the different types of withering, their effect on the various quality attributes of tea, and other aspects of withering methods that affect superior quality tea. Results: During physical withering, tea shoots loose moisture content that drops from approximately 70-80% to 60-70% (wet basis). This leads to increased sap concentration in tea leaf cells, and turgid leaves become flaccid. It also prevents tea shoots from damage during maceration or rolling. During chemical withering, complex chemical compounds break down into simpler ones volatile flavor compounds, amino acids, and simple sugars are formed. Withering increases enzymatic activities as well as the concentration of caffeine. Research indicates that about 15% of chlorophyll degradation occurs during withering. It is also reported that during withering lipids break down into simpler compounds and catechin levels decrease. Improper withering can cause adverse effects on subsequent manufacturing operations, such as maceration, rolling, fermentation, drying, and tea storage. Conclusion: Freshly harvested leaves are conditioned physically and chemically for subsequent processing. There is no specified withering duration, but 14-18 h is generally considered the optimum period. Proper and even withering of tea shoots greatly depends on the standards of plucking, handling, transportation, environmental conditions, time, and temperature. Thus, to ensure consumption of high quality tea, the withering step must be monitored carefully.

• 한국환경과학회지
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• 제21권11호
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• pp.1307-1320
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• 2012

The purpose of this paper is to provide information on planting construction for healthy plant growth. To achieve this purpose, this study analyzed the planting type, planting density, withering rate, soil characteristics, and cambium electrical resistance (CER) of withered trees in an apartment complex with a high withering rate. The major plant groups examined consisted of native broad-leaved tree species (39.3%), native narrow-leaved tree species (24.2%), and native broad-leaved - exotic narrow-leaved tree species (16.4%). The planting density of the green area, where trees were planted from 0.0 to 0.3 trees per unit area, was measured as 98.4%. Withered trees were found in 19 of the 20 planted species, and the withering rate was 41.8% (610 withered/1,461 planted). Withering rates for tree species were measured as follows: Sophora japonica and Salix babylonica (100.0%), Magmolia denudata (84.3%), Lindera obtusiloba (74.7%), cornus kousa (69.3%), acer triflorum (69.2%), diospyros kaki (66.7%), Prunus yedoensis (62.8%), Acer palmatum (52.6%), Prunus armeniaca (51.1%), Chaenomeles sinensis (43.7%), Ginkgo biloba (40.9%), Zelkova serrata (31.0%), Cornus officinalis (28.6%), Taxus cuspidata (25.6%), Pinus densiflora (21.4%), Pinus parviflora (15.2%), Pinus strobus (14.6%), and Abies holophylla (10.3%). Soil chemical analyses for 18 samples revealed that as the withering rate increased, the following occurred: (a) the ratio of silt and clay in soil increased; (b) the soil pH, organic matter rate, nitrogen, available phosphorus, and cation exchange capacity (CEC) in samples were graded as "inadequate," based on the plant grading evaluation; and (c) the NaCl and cation exchange capacity were evaluated as "somewhat satisfactory." The measurement of CER for withering rate shows electrical resistance for higher withering rate are higher, which could predict that a tree will not grow well.

• 한국약용작물학회지
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• 제8권1호
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• pp.58-63
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• 2000

작약 식재후(植載後) 1년생은 일반관리법으로 재배하고 2년생부터 4년생 까지 3년간은 지상부 병해(病害)에 약제(藥劑)를 전혀 살포하지 않고 조기고사된 작약과 1년에 4회씩 3년간 적용약제를 살포하여 정상생육한 작약을 7월 25일부터 10월 25일까지 한 달 간격으로 4회 수확하여 조사한 결과 약제무방제에 따른 조기고사의 경우 건근수량(乾根收量)이 7월 하순경에 일찍 수확한 것은 1,126kg/10a 로 10월 하순 수확 1,177kg/10a에 비해 차이가 없으면서 paeoniflorin 함량이 가장 높았고, 약제방제에 따른 정상생육의 경우 수확 시기를 조정하여 10월 하순에 늦게 수확하는 것이 paeoniflorin 함량은 7, 8월 수확보다 낮았지만 건근수량(乾根收量)은 1,650kg/10a로 7월 수확 1,242kg/10a 에 비해 33% 증수(增收)되었다. 따라서 고품질 다수확을 위해서 는 작약 지상부병해에 약제를 살포하지 않고 일찍 고사시킬 것이 아니라 약제방제를 철저히 하여 지상부 생육을 10월 하순까지 유지하는 것이 필수적이었다.

• 한국초지조사료학회지
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• 제21권4호
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• pp.217-224
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• 2001

In order to identify the growth characterization, the yield and chemical composition of Jeju barnyard grass (Echinochloa crusgalli var. fiurnentacea(Roxb) Wight) based on seeding date in Jeju region, seeding carried out the 10-day intervals from March 27 to September 30 in 2000, respectively. Plant height was 143.2 cm, showing the highest on seeding date, April 6 among that of any other seeding date. On the other hand, those of early and late seeding gradually decrease. Plant height was 119.2 an in May 16 seeding. The results of stem diameter, number of withering leaves, number of leaves and fresh weight per plant were similar to those of the plant heights. The yield of fresh, dry matter forage, crude protein and TDN found the highest on April 6 seeding, 63.5 MT/ha, 13.9 MTha, 1.1 MT/ha, and 7.6 MT/ha, respectively. In early and late seeding, the yield was gradually decreased. In seeding May 16, the yield found .38.2 MTIha in fresh forage, 6.2 MTha in dry matter forage, 0.7 MT/ha in crude protein and 3.7 MTha in TDN, respectively. According to delaying the seeding date, March 27 to May 16, the contents of crude protein (from 7.9 to 10.8%), ether extract (from 4.6 to 6.0%), nitrogen free extract (from 45.1 to 46.5%), and TDN (from 54.2 to 60.8%) were gradually increased, respectively. On the other hand, those of crude fiber (from 28.9 to 25.6%) and crud ash (from 13.5 to 11.2%) were decreased. These results showed that April 6 was the optimum seeding date with the sole object of feed production of Jeju barnyard grass under the environmental condition like as atmospheric phenomena and soil in Jeju region. (Key words : Jeju barnyard grass, Seeding date, Forage yield, Chemical composition)

• 한국작물학회지
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• 제45권3호
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• pp.163-166
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• 2000

본 연구는 제주도에 있어서 인산 시비량 차이(0, 5, 15, 25, 35 kg/10a)에 따른 차풀의 생육반응, 수량성 및 사료가치를 검토하고, 적정 인산 시비량을 구명하기 위하여 1998년 3월부터1998년 9월까지 시험하였다. 개화기까지의 일수는 인산 시비량이 증가됨에 따라 점차적으로 지연되었다 초장, 본당 분지수 및 엽수, 경직경, 엽록소 측정치 등은 인산 시비량이 증가됨에 따라 점차적으로 증가하였으나, 본당 고엽수는 적어지는 경향이었다. 생초수량은 무인산구에서 3,291kg/10a이었으나 인산 시비량이 많아짐에 따라 점차적으로 증가되어 25kg/10a과 35kg/10a 시비구에서는 각각 10a당 5,200 kg과 5,230kg이었으나, 두처리간에는 유의성이 없었다. 건초, 조단백질 및 TDN수량도 생초수량의 변화와 비슷한 경향이었다. 조단백질, 조지방, 가용성무질소물, TDN 함량은 인산 시비량이 증가됨에 따라 많아지는 경향이었으나, 조회분과 조섬유 함량은 낮아졌다.

• The Plant Pathology Journal
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• 제24권4호
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• pp.453-460
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• 2008

In the course of in vitro and in vivo screening for bioherbicidal agents, a hyphomycete fungus, Myrothecium sp. F0252 was selected as a candidate for the biocontrol of weeds. The isolate was identified as Myrothecium roridum Tode ex. Fries based on the morphological characteristics and 18S ribosomal DNA sequence analysis and registered as Myrothecium roridum F0252. In order to evaluate the in vitro effect of M. roridum F0252 on germination of ladino clover and white clover (Trifolium repens L.) seeds, spore solution of the fungus was employed in two concentrations, $6.5{\times}10^6$ and $2.5{\times}10^7$ spores per mL and then inoculated to the seeds. The fungal spores inhibited the seed germination, infected the seedlings, and caused an abnormal withering and inhibition of seedling growth. In addition, when the herbicidal activity of crude ethyl acetate extract from the liquid culture was assessed on a mini-plant, duck-weed (Lemna paucicostata (L.) Hegelm.), the extract showed high inhibitory effect at the level of $12.5{\mu}g$ per mL. On the other hand, in vivo herbicidal activity of M. roridum F0252 was evaluated by a whole plant spray method. M. roridum F0252 exhibited strong and broad-spectrum herbicidal activity. The herbicidal values ranged from 95-100% against 7 weeds, including Abutilon avicennae and Xanthium strumarium, and 70-80% against Digitaria sanguinalis and Sagittaria pygmaea. When the nutritional utilization (95 carbon sources) pattern of M. roridum F0252 was investigated, it varied with water activity ($a_w$) and temperature conditions, supplying good, basic information in regard to nutritional utilization for proper cultivation and formulation. Our results showed that M. roridum F0252 might be used as a potential biocontrol agent against weedy plants.

• 한국초지조사료학회지
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• 제21권2호
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• pp.59-66
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• 2001

In order to determine the influence of nitrogen on agronomic characters, forage yield and quality, a Kenaf was cultured on the volcanic ash soil at the Experimental Farm of Cheju national University under the seven levels of nitrogen rates(0, 50, 100, 150, 200, 250kg/ha) from April 25 to Dec. 25, 1999. The plant height increased by increase of nitrogen rate, showing longest 250kg/ha with 286.6cm and shortest at no application plot with 255.7cm. The difference on leaf number, leaf withering number, stem diameter and branches number by nitrogen rate showed a similar tendency to the plant height. Increasing N rate from 0 to 250kg/ha fresh forage yield form 55.8 to 99.8MT/ha, dry matter (DM) yield from 8.8 to 15.8MT/ha, crude protein(CP) yield form 1.2 to 3.1MT/ha, total digestible nutrients (TDN) yield from 3.8 to 8.6MT/ha. However, no significant differences in these yields were found between 200 and 250kg N/ha. Nitrogen uptake increased form 192.9 to 496.2 kg/ha but N use efficiency decreased form 95.0 to 66.6 kg DM$^{a}$ /kg N with increasing from 0 to 250 kg/ha. As N rate increased from 0 to 250kg/ha, leaf and stem out of CP, crude fat (CF), nitrogen free extract (NFE), TDN contents increased from 20.1% to 25.8% and from 9.7% to 12.4%, from 5.6% to 8.1% and from 3.3% to 4.4%, from 36.1% to 40.2% and from 21.9% to 32.4%, from 59.3% to 75.0% and from 32.2% to 38.2%, respectively, while leaf and stem out of crude fiber decreased from 18.5% to 16.7% and from 51.5% to 39.3%. Based on the these findings, the optimum N rate for forage production of kenaf seems to be about 200 kg/ha in atmospheric phenomena and volcanic ash soils of jeju island.

• Journal of Ginseng Research
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• 제45권1호
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• pp.58-65
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• 2021

Background: Panax ginseng, as one of the most widely used herbal medicines worldwide, has been studied comprehensively in terms of the chemical components and pharmacology. The proteins from ginseng are also of great importance for both nutrition value and the mechanism of secondary metabolites. However, the proteomic studies are less reported in the absence of the genome information. With the completion of ginseng genome sequencing, the proteome profiling has become available for the functional study of ginseng protein components. Methods: We optimized the protein extraction process systematically by using SDS-PAGE and one-dimensional liquid chromatography mass spectrometry. The extracted proteins were then analyzed by two-dimensional chromatography separation and cutting-edge mass spectrometry technique. Results: A total of 2,732 and 3,608 proteins were identified from ginseng root and cauline leaf, respectively, which was the largest data set reported so far. Only around 50% protein overlapped between the cauline leaf and root tissue parts because of the function assignment for plant growing. Further gene ontology and KEGG pathway revealed the distinguish difference between ginseng root and leaf, which accounts for the photosynthesis and metabolic process. With in-deep analysis of functional proteins related to ginsenoside synthesis, we interestingly found the cytochrome P450 and UDP-glycosyltransferase expression extensively in cauline leaf but not in the root, indicating that the post glucoside synthesis of ginsenosides might be carried out when growing and then transported to the root at withering. Conclusion: The systematically proteome analysis of Panax ginseng will provide us comprehensive understanding of ginsenoside synthesis and guidance for artificial cultivation.

• 한국초지조사료학회지
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• 제21권4호
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• pp.225-232
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• 2001

본 시험은 제주피의 적정 파종량을 구명하기 위하여 2000년 4월 16일에 ha당 15, 20, 25, 30, 35, 40kg에 해당하는 량을 조파하여 2000년 6월 28일(1차 예취)과 8월 30일(2차 예취)에 생육특성, 사초수량, 사료성분 등을 조사하였다. 초장은 15kg/ha 파종구에서 155.5cm였던 것이 파종량이 35kg/ha으로 증가함에 따라 162cm로 커졌으나, 그 이상 파종량을 증가할수록 작아져서 40kg/ha 파종구에서는 152.7cm였다. 경직경. 엽수, 마디수 및 개체당 무게는 파종량이 많을수록 감소하였다. 파종량이 15kg/ha에서 35kg/ha로 증가됨에 따라 생초수량은 61.0MT/ha에서 73.0MT/ha로, 건물 수량은 11.9MT/ha에서 16.9MT/ha로, 단백질 수량은 0.9MT/ha에서 1.6MT/ha로, TDN수량은 6.1MT/ha에서 9.7MT/ha로 증가되었다가 그 이상으로 파종량을 증가시킬수록 감소되었다. 파종량이 15kg/ha에서 40kg/ha으로 증가함에 따라 조단백질 함량은 7.4%에서 10.4%로 조지방 함량은 3.3%에서 5.2%로, 가용무질소물 함량은 46.4%에서 47.8%로 TDN함량은 52.1%에서 60.4%로 증가되었으나, 조섬유함량은 30.3%에서 27.6%로 조회분 함량은 12.7%에서 9.0%로 감소되었다. 이상의 결과로 보아 사초수량을 최고로 올릴 수 있는 파종량은 35kg/ha로 추정할 수 있었다.

• 한국작물학회지
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• 제46권6호
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• pp.439-442
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• 2001

제주지역에서 파종기이동(4월 25일, 5월 10일, 5월 25일, 6월 10일, 6월 25일)에 따른 양마의 생육특성, 수량성 및 사료 가치를 구명하기 위하여 1999년 4월 25일에서 6월 25일까지 15일 간격으로 시험하였다. 1. 초장은 4월 25일 파종구에서 278.6 cm 였던 것이 파종기가 지연됨에 따라 점차적으로 작아져서 6월 25일 파종에서 초장은 205.7 cm로 짧아졌다. 엽수, 고엽수, 경직경, 분지수 및 개체당 무게는 초장과 비슷한 경향이었다. 2. 생초, 건초, 단백질 및 TDN수량은 4월 25일 파종구에서 각각 98.5MT/㏊, 20.7MT/㏊, 2.9MT/㏊, 11.6MT/㏊로 가장 높았으나 만파할수록 점차적으로 감수되어 6월 25일 파종에서 생초수량은 45.9MT/㏊, 건초수량은 8.2MT/㏊, 단백질수량은 1.3MT/㏊, TDN수량은 5MT/㏊로 감수되었다. 엽과 경의 생초, 건초, 단백질 및 TDN수량도 만파할수록 감소되었다. 3. 파종기가 4월 25일에서 6월 25일로 지연됨에 따라 엽의 단백질 함량은 21.5%에서 24.4%로, 조지방 함량은 5.2%에서 6.1%로, NFE 함량은 39.9%에서 41.2%로, TDN 함량은 64.3%에서 69.7%로 증가한 반면에 조섬유 함량은 엽에서 20.8%에서 17.5%로, 조회분 함량은 7.9%에서 7.0%로 감소되는 경향이었다. 경의 조단백, 조지방, NFE, TDN함량은 만파 할수록 증가되었으나 조회분 및 조섬유 함량은 반대의 경향이었다. 이상의 결과로 보아 제주도 기상, 토양의 환경조건하에서 양마의 파종적기는 4월 25일로 판단되었다.