• Genes and Genomics
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• v.40 no.12
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• pp.1339-1349
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• 2018

Cerebral palsy (CP) is a non-progressive neurological disease, of which susceptibility is linked to genetic and environmental risk factors. More and more studies have shown that CP might be caused by multiple genetic factors, similar to other neurodevelopmental disorders. Due to the high genetic heterogeneity of CP, we focused on investigating related molecular pathways. Ten children with CP were collected for whole-exome sequencing by next-generation sequencing (NGS) technology. Customized processes were used to identify potential pathogenic pathways and variants. Three pathways (axon guidance, transmission across chemical synapses, protein-protein interactions at synapses) with twenty-three genes were identified to be highly correlated with CP. This study showed that the three pathways associated with CP might be the molecular mechanism of pathogenesis. These findings could provide useful clues for developing pathway-based pharmacotherapies. Further studies are required to confirm potential roles for these pathways in the pathogenesis of CP.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.42 no.3
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• pp.1-6
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• 2010

A new testing method named pitch deposit tester (PDT) was developed by KRICT in order to evaluate the deposit potential of micro-stickies. The new method involves depositing the potential pitch particles on the air bubble covered plastic film set in the pitch deposit tester (PDT) and analysing the deposited area by an image analyzer. In this study, the effect of fixing agents on potential pitch deposition was elucidated. The effects of some fixing agents (polyamine and polyethyleneimine) on pitch control were investigated by the PDT test of 100% recycled newsprint stock. The study suggested that proper use of the PEI can lead to better pitch control than that of polyamine. The efficiency of novel screening method using the PDT and retention and drainage analyser (RDA) for fixing agents in terms of retention and deposit contamination could be confirmed by above mentioned results.

• Microbiology and Biotechnology Letters
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• v.47 no.1
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• pp.69-77
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• 2019

Screening microorganisms that can produce glucan hydrolases for industrial, environmental, and biomedical applications is important. Herein, we describe a novel approach to perform glucan hydrolase screening-based on analysis of matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) spectra-which involves degradation of the oligo- and polysaccharides. As a proof-of-concept study, glucan hydrolases that could break down glucans made of several glucose units were used to demonstrate the MALDI-MS-based enzyme assay. First, the enzyme activities of ${\alpha}$-amylase and cellulase on a mixture of glucan oligosaccharides were successfully discriminated, where changes of the MALDI-MS profiles directly reflected the glucan hydrolase activities. Next, we validated that this MALDI-MS-based enzyme assay could be applied to glucan polysaccharides (i.e., pullulan, lichenan, and schizophyllan). Finally, the bacterial glucan hydrolase activities were screened on 96-well plate-based platforms, using cell lysates or samples of secreted enzyme. Our results demonstrated that the MALDI-MS-based enzyme assay system would be useful for investigating bacterial glucoside hydrolases in a high-throughput manner.

• Korean journal of applied entomology
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• v.34 no.4
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• pp.328-333
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• 1995

Susceptibilies of tow-spotted spider mite (Tetranychus urticae Koch) collected at 6 locations were assessed to 15 acaricides. The response to acaricides were almost similar in female adults and eggs. There were considerable difference in susceptibility depending on the acaricide treated and the region from which the population was collected. The population showing resistance ratio of more than 20 with respect to certain acaricide was regarded as a resistant population to the acaricide. The resistant populations in terms of female adult were as follows: Suwon population to azocyclotin, cyhexatin, and fenbutatin-oxide; Taejon population to dicofol and fenbutatin-oxide; Chongju population to dicofoll Chinju populatin to cyhexatin, dicofol, and fenbutatin-oxide. The resistant populations in terms of egg were as follows; Suwon population to bifenthrin, clofentezine, hexythiazox, and tetradifon; Kunwi, Chongju, and Kwangju populations to bifenthrin; Taejon population to amitraz and bifenthrin; Taejon populatin to amitraz and bifenthrin; Chinju population to amitraz, bifenthrin, clofentezine, dicofol, and tetradifon. However, the female adults and eggs of all field populations were susceptible to abamectin, chlorfenson, and fenpyroximate. This tendency was also reported previously in the susceptible strain from laboratory.

• Research in Plant Disease
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• v.22 no.3
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• pp.152-157
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• 2016

Root-dipping inoculation method has been used to investigate resistance of radish plants to Fusarium wilt. However, the method requires a lot of labor and time because of complicate procedure. This study was conducted to establish a simple and effective mass-screening method for resistant radish to Fusarium wilt. Radish seedlings of susceptible and resistant cultivars were used to investigate wounding method by scalpel, inoculum concentration, and pathogen-inoculated growth stage of seedlings. We established an efficient mass-screening method based on our results as following: Roots of 14-day-old seedlings of radish are cut with a scalpel at a $90^{\circ}$ angle to a 2 cm-depth at a 1 cm-distance from main stem and then inoculated by pouring with a 10 ml-aliquot of a fungal spore suspension ($1.0{\times}10^7conidia/ml$) on soil. The inoculated plants are cultivated in a growth room at $25^{\circ}C$ for about 4 weeks with 12-hour light a day. The proposed screening method enables to effectively select resistant from mass radish plants cultivars to Fusarium wilt.

• Korean Journal of Weed Science
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• v.15 no.3
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• pp.188-196
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• 1995

Blackgrass and water foxtail are troublesome weeds in a cultivation area of cereals. To know whether water foxtail could substitute for blackgrass as a plant material of screening for the discovery and development of new cereal herbicides, they were compared in a greenhouse and a growth chamber with respect to initial growth characteristics and responses to various herbicides. Blackgrass had a higher germination rate at lower temperature and a higher growth rate at higher temperature as compared to water foxtail. In addition, blackgrass was less sensitive to long day than water foxtail. These results suggest that blackgrass grows better in a cultivation area in spring than water foxtail. As compared to water foxtail, blackgrass showed shightly susceptible responses to herbicides when they were applied to soil surface at one day after seeding. However, the responses to the herbicides of blackgrass and water foxtail were similar with a foliar application of the herbicides at 3 or 4 leaf stage of the plant. Since there were no considerable differences in the herbicidal responses of the two plant species, water foxtail could substitute for blackgrass as a plant material for a primary herbicide screening. When water foxtail was used in a post-emergence screening test, seeding amount of 0.1g/$350cm^2$ and postemergence application of herbicides at 3 to 5 leaf stage were found to be appropriate on the view of alleviating screening efforts in a greenhouse.

• Journal of Microbiology and Biotechnology
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• v.16 no.3
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• pp.487-490
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• 2006

Because of consumer rejection of chemical pesticides and the appearance of microorganisms that are resistant to fungicides, we tried to discover biopesticides. Of 13 microorganisms isolated from Shrimp-jeotkal, a Bacillus sp. showed strong activity against tomato late blight caused by Phytophthora infestans. Its activity was tested both in vivo and in vitro. The identification of the strain was carried out based on 16S rDNA analysis and the morphology by scanning electron microscopy.

• Journal of Microbiology and Biotechnology
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• v.12 no.3
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• pp.355-359
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• 2002

The Plackett-Burman experimental design was employed to evaluate the relative importance of medium constituents of each medium for enhanced griseofulvin production by Penicillium griseofulvum MTCC 1898 and Penicillium griseofulvum MTCC 2004. It was found that the medium constituents, sucrose, $K_2HPO_4,\;NaNO_3,\;and\;FeSO_4{\cdot}7H_2O$, significantly influenced the griseofulvin production by Penicillium griseofulvum MTCC 1898. In the case of Penicillium griseofulvum MTCC 2004, lactose, glucose, and $MnSO_4{\cdot}H_2O$ significantly influenced the griseofulvin production.

• Bulletin of the Korean Chemical Society
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• v.31 no.6
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• pp.1609-1614
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• 2010

Acetylcholinesterase reactivators are crucial antidotes for the treatment of organophosphate intoxication. Standard in vitro test was chosen using a rat brain homogenate as the source of AChE. Screening of reactivation potency was performed with two concentration of reactivator (1000 ${\mu}M$ and 10 ${\mu}M$). Results were compared to established reactivators pralidoxime, methoxime, HI-6, trimedoxime and obidoxime. More than 30 novel reactivators performed equal or better reactivation ability of POX-inhibited AChE compared to currently used reactivators. The structure-activity relationship for reactivators of paraoxon-inhibited AChE was developed.

• 한국가스학회:학술대회논문집
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• 2002.05a
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• pp.46-49
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• 2002