• Journal of the Korea Organic Resources Recycling Association
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• v.7 no.1
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• pp.31-42
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• 1999

To investigate the effects of the composts made of sewage sludges on the physico-chemical properties of soil and growth of paddy rice, the variations of chemical properties in soil, the contents of inorganic matters and heavy metals in plant tissues, the contents of heavy metals in brown rice, the growth response of paddy rice and grain yield were investigated. The results were summarized as follows : The contents of organic matters, N, available $P_2O_5$ and exchangeable K in soil were increased by application of the composts. The contents of N and available $P_2O_5$ in soil were remarkably decreased at the harvesting time in comparing with the 20th day after application of the composts. The contents of heavy metals in soil were increased very slightly by application of the com-post. At the harvesting time, the contents of N and K were not varied in all plant tissues at the plot of the compost in comparing with the plot of the NPK. The content of $P_2O_5$ in plant was increased by application of the composts. The contents of heavy metals in plant tissues and brown rice were not changed among the treatments. When the composts were applied to the pot at the rates of 1,000, 2,000, 4,000 and 6,000kg/10a with NPK, the numbers of grain yield were increased with 3, 18, 44 and 54% in comparing with NPK plot, respectively. Dry weight, the numbers of tillers and spikelet of the rice plant were increased by application of the composts.

• Journal of Korean Society of Forest Science
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• v.77 no.3
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• pp.259-268
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• 1988

For the purpose of examining the effects of simulated acid rain on nutrient contents of plant tissues in Pintos densiflora seedlings and Forsythia koreana rooted cuttings, the experimental design of randomized block arrangement with three replications was implemented in the experimental field of Yesan National Agricultural Junior College. One-year-old Pinus densiflora seedlings and Forsythia koreana cuttings were planted in the pots filled the mixed soils(nursery soil : forest soil of siliceous sandy loam=1 : 1 v/v) in the early spring of 1986. The regime of artificial acid rain, in terms of spray frequency per month and spray amount at single treatment per plot, was simulated on the basis of climatological data averaged from 30 years records. Simulated acid rain(pH 2.0, pH 4.0, and pH 5.5 as control) containing sulfuric and nutric acid in the ratio of 3 : 2(chemical equivalant basis) diluted with ground water were treated on the experimental plants under condition of cutting off the natural precipitation with vinyl tunnel, during the growing season(May 1 to August 31) in 1986. The results obtained in this study were as follow : (1) As for the nitrogen contents in plant tissues, P. densiflora increased significantly in one-year-old stembranch and root tissues, and F. koreana increased significantly in leaf and root tissues, as the pH levels of acid rain decreased. (2) The available phosphate contents in root tissues of P. densiflora, and in leaf and root tissues of F. koreana were significantly decreased, as the pH levels of acid rain decreased. (3) $K_2O$, CaO and MgO contents in plant tissues were significantly decreased in the both species as the pH levels decreased. And the effects of acid rain on F. koreana were higher than those of P. densiflora. (4) Sulfur contents of plant tissues in the both species were increased at pH 2.0 treatment. There were significant differences among three acid rain treatments in leaf and root tissues of P. densiflora, and in all parts of F. koreana. (5) In the effects of simulated acid rain on the both species and the tested soils, in general, F. koreana revealed higher sensitiveness than P. densiflora, and the lower pH levels of simulated acid rain were treated, the more sharp reaction was showed.

• Horticultural Science & Technology
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• v.28 no.6
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• pp.948-953
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• 2010

We evaluated the bioactive compounds including carbohydrates (CH), organic acids (OA), ascorbic acid (AA), anthocyanin (AC), and ellagic acid (EA) in the achenes, epidermis, and flesh of fully ripe strawberry fruits of the 'Maehyang', 'Seolhyang', 'Keumhyang', 'Akiheme', and 'Red pearl' cultivars. The total contents of CH, OA, AA, and AC were significantly greater in the epidermis than in other tissues, while the EA content was significantly greater in the achenes than in other tissues. The AA content of the epidermis ranged from $49.1-69.2mg{\cdot}100g^{-1}$ FW and was significantly greater than the content of the flesh, while AA was not detected in the achenes of any of the tested strawberry cultivars. The AA contents of the epidermis and flesh of the 'Maehyang' were 69.2 and $42.2mg{\cdot}100g^{-1}$ FW, respectively, and were greater than those of the other cultivars. The AC contents in the epidermis and achenes of the 'Keumhyang' were $74.0mg{\cdot}100g^{-1}$ FW and $36.7mg{\cdot}100g^{-1}$ DW, respectively, greater than those of the other cultivars. The EA content of the achenes of the 'Seolhyang' was $215.5mg{\cdot}100g^{-1}$ DW, significantly greater than those of the other cultivars. Results indicate that the antioxidant levels and other chemical compounds of strawberry fruits vary significantly among different fruit tissues. Our results also suggest that the recently introduced Korean 'Maehyang', 'Seolhyang', and 'Keumhyang' contain higher levels of antioxidants than other major June-bearing strawberry cultivars. These cultivars are feasible selections for both growers and consumers.

• The Plant Pathology Journal
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• v.24 no.2
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• pp.171-177
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• 2008

In this study, we investigated the induced defense response and protective effects against Stemphylium vesicarium by application of benzothiadiazole ($Bion^{(R)}$) and di-potassium phosphate salt $(K_2HPO_4)$ to onion. Onion leaves were sprayed with $Bion^{(R)}$ and $K_2HPO_4$, then inoculated 2 days later with a virulent strain of S. vesicarium under greenhouse conditions. Disease severity and activities of peroxidase (PO), polyphenoloxidase, phenylalanine ammonia-lyase (PAL) and phenol contents were evaluated in the treated leaf tissues. Reduction in the disease severity was observed in plants treated with $Bion^{(R)}$ and $K_2HPO_4$. Onion plants treated with $Bion^{(R)}$ and $K_2HPO_4$ and inoculated with the pathogen showed significantly higher PAL activity, PO activity, and phenol contents than inoculated water-treated plants 2 days after the treatment. In conclusion, the results of this study provide evidence that application of simple non-toxic chemical solutions as di-potassium phosphate and $Bion^{(R)}$ can control Stemphylium leaf blight of onion.

• Korean Journal of Environmental Agriculture
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• v.16 no.3
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• pp.220-226
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• 1997

To study the possibility of agricultural utilization of the municipal sewage and the industrial wastewater sludges, the chemical properties of soil, the growth response of corn plant and uptake of inorganic nutrients in plant tissues were investigated by application of the composts made of the sludges to the pots at the rate of 2,000kg/10a. The contents of organic matter, T-N, $P_2O_5$ and K in soil were increased by application of the composts. Each heavy metals contents in soil were increased very slightly by application of the composts. The fresh weights of corn plant were increased with $9{\sim}19%\;and\;60{\sim}79%$ at the plot of compost and NPK+composts in comparing with the plot of NPK, respectively. Uptake of N, $P_2O_5,\;K_2O$, CaO and MgO in stem and leaf of corn plant were increased by applications of the composts.

• Korean Journal of Soil Science and Fertilizer
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• v.33 no.6
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• pp.493-497
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• 2000

An experiment was conducted to evaluate the possibility of using basic oxygen furnace (BOF) slag as soil conditioner in soybean upland field. In 1997, soybean (Glycine max L. cv. Eunha) crop was cultivated under different application rates of BOF slag at an experimental field of Chonnam Rural Development Administration in Nampyung, Najoo city. Five treatments, four application rates of BOF slag (0, 4, 8, $12Mg\;ha^{-1}$) and one application rate of lime ($2Mg\;ha^{-1}$) were tried with three replications. Plant height and shoot dry weight per plant were measured five times during the growth period. Chemical contents of soybean plant tissues and soil were also measured at the same sampling date. Yield were estimated by harvesting $6.6m^2$ per experimental unit and yield components were measured by sampling 10 plants per experimental unit at the harvest date. In upland soil, application of BOF slag rarely affected contents of total nitrogen, organic matter, available phosphate and potassium in soil. Soil pH, and contents of Ca and Fe in soil became higher as BOF slag rate increased. Enhancement of soil pH by application of BOF slag appeared to be closely related with increase in soil Ca content. Application rate of $2Mg\;ha^{-1}$ of lime showed almost the same effect in increase of soil Ca content as application rate of $4{\sim}8Mg\;ha^{-1}$ of BOF slag. Slag treatment hardly affected the contents of total nitrogen, $P_2O_5$, CaO, $K_2O$ and MgO in the shoot of soybean plants. Soybean plants under treatments of BOF salg showed better growth from the earlier growth stage compared with those of control treatment, and at the later growth stage, their growth was even superior to that of lime treatment. BOF slag rate of $8Mg\;ha^{-1}$ showed the highest soybean yield with $1,232kg\;ha^{-1}$. which was $330kg\;ha^{-1}$ or 37% higher than the yield of control with $902kg\;ha^{-1}$, As a result, BOF slag appeared to be useful material as a soil conditioner as well as nurient source for Ca and Fe in upland soybean fields, and its optimal rate for higher yield seemed to be around $8Mg\;ha^{-1}$.

• Korean Journal of Weed Science
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• v.17 no.3
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• pp.314-324
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• 1997

This study was conducted to investigate whether 1) photosynthetic pigments(chlorophylls and carotenoids) are formed in dodder plant(Cuscuta australis), 2) there are any characteristics in the pigment biosynthesis, compared to that of other normal plants, and 3) dodder responds to some herbicides having target site on chloroplast. 1. Chlorophyll content of dodder tendrill grown under a natural daylight was 9 times and 50 times lower than that of field bindweed stem and leaf, respectively. 2. The photosynthetic pigment contents varied in different tissues, being higher in a apical region than in a lower region of seedling or tendrill. Chlorophyll wasn't almost observed below the 4th internode from the upper. 3. Pigment contents were greatly dependent on light intensity so that there were 4 to 6 times difference among light conditions. When the shoot containning low pigment contents under natural light, was incubated in growth chamber with various light intensities, the pigment contents were increased by 3 times of initial contents at about 97${\mu}E$ $m^{-2}s^{-1}$PAR. While the change in pigment contents was not observed at above 450${\mu}E$ $m^{-2}s^{-1}$PAR 4. Exogenous supply of 5mM 5-aminolevulinic acid increased protochlorophyllide by 7 times and 1.4 times in the etiolated shoot from field bindweed rhizome and in dodder stem, respectively, showing that dodder relatively has a low response to 5-aminolevulinic acid. 5. Pigment loss was observed in the treatment of paraquat, norflurazon, oxyfluorfen and diuron, and protoporphyrin IX was accumulated by oxyfluorfen as in normal plants Based on above results, several chracteristics of pigment biosynthesis in dodder seem to be summerized as follows. Photosynthetic pigment biosynthesis in Cuscuta australis runs even in low level. The pigment contents is differentially distributed in different regions and their contents seem significantly to be controlled by light intensities. Especially, chloroplast rapidly tends to degenerate with the development of tissue. Some herbicides having target site on chloroplast induce damage to dodder stem but are unlikely to control it well in field, except paraquat, due to low chloroplast activity and parasitic mode of nutrition.

• Korean Journal of Organic Agriculture
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• v.16 no.3
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• pp.287-298
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• 2008

This experiment was carried out to investigate the effect of different feed conditions on growth, cast production and conversion efficiency of organic matter to the earthworm. The experiment was tested on cow manure(CM) mixed with rice hulls (RH) or rice straw(RS). The mixture ratios were designed as one time, two times and three times of cow manure volume, respectively. The CM and RH mixtures resulted better on growth rate, reproductive efficiency and wormcast production than those for CM and RS mixtures. Especially on three times of RH mixture showed the highest growth characteristics compared to the other mixtures. Although both were between 20 and 34 on carbon and nitrogen ratio, the result inferred that the difference on the growth might have been caused by feeding conditions. On the mixtures of cow manure with rice hulls(CM+RH) was significantly higher on values on the conversion rate and conversion efficiency of organic matter to earthworm tissues than the mixtures of cow manure with rice straw(CM+RS). The most concerned point of the mixture of earthworm feed is that high contents of volatile solid and total carbon that increases in conversion efficiency of organic matter to earthworm tissues, and also causes the increase in growth rate and reproductive efficiency. The wormcast could be used as a valuable plant growth medium or soil conditioner for sustainable agriculture and it may be due to their high qualities of physico-chemical properties.

• Korean Journal of Ecology and Environment
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• v.41 no.2
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• pp.188-197
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• 2008

This research was a preliminary case study to determine the levels of total mercury in the tissues of sentinel species (Zacco platypus) and ecological health in relation to habitat characteristics and chemical conditions. We collected fishes in Gap Stream during June$\sim$October 2007 and analyzed the total mercury from five types of tissues such as liver, kidney, gill, vertebrae and muscle of Zaceo platypus using Direct Mercury Analyzer (DMA-80, US EPA Method 7473). Mean concentrations of total [Hg], based on all tissues, was 67.2 and $20.7\;{\mu}g\;kg^{-1}$, in the upstream and downstream site, respectively, indicating 3 times greater level in the upstream. In other words, the levels were higher in the pristine upstream than the downstream influenced by the wastewater disposal plant. Chemical water quality, based on BOD, COD and nutrients (TN, TP) showed that severe degradation occurred in the downstreams than the upstreams. Index of Biological Integrity (IBI) using fish multi-metric model averaged 32, indicating a "good$\sim$fair" condition and varied from 42 (excellent$\sim$good) at S2 to 22 (fair$\sim$poor) at S5 depending on the sites sampled. Qualitative Habitat Evaluation Index (QHEI) in the all sites averaged 142, which was judged as "good" habitat health, but showed a high variation (181 in Site 2 vs. 67 in Site 5). Overall data suggest that health conditions, based on IBI and QHEI, was better in the upstream sites but the mercury bioaccumulation levels in the fish tissues were opposite. We believe that measurements of various parameters are required for a diagnosis of integrative ecosystem health.

• Journal of Wetlands Research
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• v.9 no.1
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• pp.69-78
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• 2007

Constructed wetlands and other aquatic systems have been successfully used for waste and wastewater treatment in either temperate or tropical regions. To treat waste or wastewater in a sustainable manner, the integrated eco-engineering designs are explained in this paper with 2 case studies: (i) a combination of vertical-flow constructed wetland (CW) with plant irrigation systemfor fecal sludge management and (ii) integrated CW units with landscaping at full-scale application for domestic wastewater treatment. The pilot-scale study of fecal sludge management employed 3 vertical-flow CW units, each with a dimension of $5{\times}5{\times}0.65m$ (width ${\times}$ length ${\times}$ media depth) and planted with cattails (Typha augustifolia). At the solid loading rate of 250 kg total solids (TS)/$m^2.yr$ and a 6-day percolate impoundment, the CW system could achieve chemical oxygen demand (COD), TS and total Kjeldahl nitrogen (TKN) removal efficiencies in the range of 80 - 96%. The accumulated sludge layers of about 80 - 90 cm was found at the CW bed surface after operating the CW units for 7 years, but no clogging problem has been observed. The CW percolate was applied to 16 irrigation Sunflower plant (Helianthus annuus) plots, each with a dimension of $4.5{\times}4.5m$ ($width{\times}length$). In the study, the CW percolate were fed to the treatment plots at the application rate of 7.5 mm/day but the percolate was mixed with tap water at different ratio of 20%, 80% and 100%. Based on a 1-year data of 3-crop plantation were experimented, the contents of Zn, Mn and Cu in soil of the experimental plots were found to increase with increasing in CW percolate ratios. The highest plant biomass yield and oil content of 1,000 kg/ha and 35%, respectively, were obtained from the plots fed with 20% or 50% of the CW percolate, whereas no accumulation of heavy metals in the plant tissues (i.e. leaves, stems and flowers) of the sunflower is found. In addition to the pilot-scale and field experiments, a case study of the integrated CW systems for wastewater treatment at Phi Phi Island (a Tsunami-hit area), Krabi province, Thailand is illustrated. The $5,200-m^2$ CW systems on Phi Phi Island are not only for treatment of $400m^3/day$ wastewater from hotels, households or other domestic activities, but also incorporating public consultation in the design processes, resulting in introducing the aesthetic landscaping as well as reusing of the treated effluent for irrigating green areas on the Island.