• Biotechnology and Bioprocess Engineering:BBE
• /
• 제8권4호
• /
• pp.227-232
• /
• 2003

A self-assembled monolayer of protein G was fabricated to develop an immunosensor based on surface plasmon resonance (SPR), thereby improving the performance of the antibodybased biosensor through immobilizing the antibody molecules (lgG). As such, 11-mercaptoundecanoic acid (11-MUA) was adsorbed on a gold (Au) support, while the non-reactive hydrophilic surface was changed through substituting the carboxylic acid group (-COOH) in the 11-MUA molecule using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrocholide (EDAC). The formation of the self-assembled protein G layer on the Au substrate and binding of the antibody and antigen were investigated using SPR spectroscopy, while the surface topographies of the fabricated thin films were analyzed using atomic force microscopy (AFM). A fabricated monoclonal antibody (Mab) layer was applied for detecting E. coli O157:H7. As a result, a linear relationship was achieved between the pathogen concentration and the SPR angle shift, plus the detection limit was enhanced up to 10$^2$ CFU/mL.

• 한국양식학회지
• /
• 제12권1호
• /
• pp.63-69
• /
• 1999

A series of experiments were conducted to examine on what stimulus giant danio (Danio malabaricus) were mainly responsible for detecting alarm substance. When 0.15 ml alarm substance (10 ppm) was introduced into the tank, fish showed no notable change in swimming pattern. However, the introduction of 1.5 ml alarm substance (100 ppm) could induce fright reaction about in 6 minutes. Further, when 15 ml alarm substance (1,000 ppm) was introduced into the tank, fish showed the following fright reaction in a few seconds; suppressing to feed diet, no swimming, strong chasing, and visual alertness. In detecting alarm substance, fish were mainly depending on the chemical stimulus (nares) rather than the vision stimulus (eyes) because fish detected alarm substance by the chemical cue showed the significantly stronger fright reaction than by the visual cue. The time for fish to show the initial fright reaction after detecting alarm substance by the chemical cue was shorter than by the visual cue. Also after alarm substance was introduced into the tank, olfaction-deprived fish showed significantly weaker fright reaction and less frequency of chasing than the normal fish which detected alarm substance by both olfaction and gustation stimulus. These results indicated that chemical stimulus, especially olfaction might be the primary sensory modality used in the detection of the alarm substance for giant danio.

• Korean Chemical Engineering Research
• /
• 제49권4호
• /
• pp.393-399
• /
• 2011

지난 수십년 동안, 환경오염물질에 대한 광학적 전기화학적 검출 방법에 관한 다양한 연구가 진행되었다. 최근에는 저렴하고, 장치가 불필요한 육안 식별 가능한 환경색센서가 개발되고 있다. 시각적 정성분석은 대상물질에 대한 즉각적인 정보를 제공하여, 실시간 현장 분석이 가능하게 해준다. 또한 정량분석이 가능한 색센서에 대한 관심도 높아지고 있다. 환경색센서는 기상의 VOC, 액상의 중금속 분석용으로 주로 개발되고 있다. 이에 본 총설에서는 다양한 환경색센서의 활용분야를 살펴보고, 기존 색센서의 문제점을 파악한 다음 환경색센서 기술의 발전방향에 관하여 전망하였다.

• 한국군사과학기술학회지
• /
• 제25권2호
• /
• pp.210-217
• /
• 2022

Chemical warfare agents(CWA) such as nerve agents and vesicating agents show lethality by skin contamination. Skin protection, therefore, is one of the top priorities to deal with the growing threat from CWA. In an attempt to develop the most effective topical skin protectant(TSP), candidate substances including PFPE(perfluorinated polyether), PTFE(polytetrafluoroethylene), glycerin, and polysaccharides were evaluated in forms of various formulations against nerve agent simulant DMMP(dimethylmethyl phosphonate) penetration. The protective efficacy of the formulation against DMMP penetration was estimated as the onset time of color change of the KM9 chemical agent detection paper. Based on this study, it was found that several PFPE- and glycerin-based formulations exhibit remarkably superior efficacy as a protective cream. This protective cream is expected to be used as TSP for military application after further research.

• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2005년도 생물공학의 동향(XVII)
• /
• pp.769-769
• /
• 2005

• Korean Chemical Engineering Research
• /
• 제58권3호
• /
• pp.408-415
• /
• 2020

본 연구에서는 auto-encoder와 self-organizing map을 결합한 auto-encoder with self-organizing map(AE-SOM) 기법을 이용하여 EVA 생산공정의 이상을 검출 및 진단하였고, Granger의 인과분석을 통해 이상 검출 데이터의 이상 전파 방향을 확인하였다. 분석 데이터는 1년 7개월 간의 조업데이터를 이용하였으며, autoclave 반응기의 조업 변수를 주로 분석하였다. 데이터 전처리 과정에서 데이터의 표준화를 먼저 진행하고, 조업의 각 grade의 sample 수를 동일하게 200개 임의로 추출하였다. 이후 AE-SOM을 적용하여 각 grade의 best matching unit (BMU)를 도출하였다. 각각의 BMU를 기준으로 조업 데이터가 얼마나 벗어났는지를 기준으로 데이터의 이상을 판별하였다. 공정 이상이 발견될 시 이상원인을 contribution plot을 이용하여 확인하였고 이상원인 변수의 인과성을 Granger의 인과분석을 통해 분석하였다. 그 결과 조업 시 발생한 2번의 셧다운의 전조를 모두 검출하였으며 이상이 발생한 원인변수에서 기인한 공정 이상의 전파 방향을 분석하였다.

• Bulletin of the Korean Chemical Society
• /
• 제32권2호
• /
• pp.583-589
• /
• 2011

Fluorescence correlation spectroscopy (FCS) is a sophisticated and an accurate analytical technique used to study the diffusion of molecules in a solution at the single-molecule level. FCS is strongly affected by many factors such as the stability of the excitation power, photochemical processes, mismatch between the refractive indices, and variations in the cover glass thickness. We have studied FCS near the surface of a cover glass by using rhodamine 123 as a fluorescent probe and have observed that the surface has a strong influence on the measurements. The temporal autocorrelation of FCS decays with two characteristic times when the confocal detection volume is positioned near the surface of the cover glass. As the position of the detection volume is moved away from the surface, the FCS autocorrelation becomes one-component decaying; the characteristic time of the decay is the same as the faster-decaying component in the FCS autocorrelation near the surface. This observation suggests that the faster component can be attributed to the free diffusion of the probe molecules in the solution, while the slow component has its origin from the interaction between the probe molecules and the surface. We have characterized the surface contribution to the FCS measurements near the surface by changing the position of the detection volume relative to the surface. The influence of the surface on the diffusion of the probe molecules was monitored by changing the chemical properties of the surface. The surface contribution to the temporal autocorrelation of the FCS strongly depends on the chemical nature of the surface. The hydrophobicity of the surface is a major factor determining the surface influence on the free diffusion of the probe molecules near the surface.

• 대한화학회지
• /
• 제33권1호
• /
• pp.46-54
• /
• 1989

식품 및 비타민 제제중에 존재하는 지용성 비타민의 추출 및 동시 정량법을 검토하였다. 역상 액체크로마토그래피에 의한 지용성 비타민의 동시 분리 및 정량은 메탄올 : 물 = 95 : 5 이동상으로 Novapak $C_{18}$ 컬럼하에서 이루어졌다. 비타민의 검출에는 UV검출기를 사용하였으며 검출감도를 증가시키기 위하여 분석중 UV 검출파장을 각각의 비타민 용리시간에 따라 최대흡수 파장인 330, 265, 285 및 290nm로 변경하였다. 지용성 비타민의 분리 및 분석은 40분안에 완료되었다. 시료의 전처리에는 알카리 가수분해법과 효소가수분해법을 이용하였으며, 비타민의 추출은 액체-액체추출법과 액체-고체추출법을 이용하였다. 비타민 A,D, E 분석에는 알카리 가수분해법 및 효소가수분해법이 비타민 K 분석에는 효소가수분해법만이 좋은 결과를 나타냈으며, 비타민 추출에는 액체-액체 추출시 diethyl ether, pentane 및 n-hexane이, 액체-고체 추출시 silica 카트리지가 좋은 추출효과를 나타냈다.

• 대한기계학회:학술대회논문집
• /
• 대한기계학회 2008년도 추계학술대회A
• /
• pp.1617-1622
• /
• 2008

We report a new molecular detection process which measures the changes in the plasmon resonance peaks of periodic Au nanoparticle arrays fabricated using the electron beam lithography. As the Au nanoparticle arrays are modified by the chemical reaction in solutions having various concentrations of a target molecule, both the position and intensity of the plasmon peak change in proportion to the concentration of the target molecule. We expect that the process developed in this work can be employed for fine tuning of the plasmon peak wavelength and also for the optical detection of various kinds of molecules. Moreover, this method may improve the measurement accuracy compared with existing approaches that use only one change (peak wavelength or peak intensity) as a readout value for the molecular detection.

• 한국가스학회:학술대회논문집
• /
• 한국가스학회 2001년도 추계학술발표회 논문집
• /
• pp.145-149
• /
• 2001