• Biomedical Science Letters
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• v.12 no.2
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• pp.127-130
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• 2006

It is important to coordinated interaction among neurons, astrocytes and endothelial cells to maintain the function of brain. To study their regulatory mechanisms in vitro system, the co-culture system among the isolated cells from brain may be needed. However, the method for purifying brain microvascular endothelial cells (BMEC) far culture have not established yet. In this study, the proper culture methods of mice cells using two different strains, CD1 and C57BL6, to obtain the pure and plentiful endothelial cells were described. The flatted-round forms of CD1 endothelial cells grew on the collagen-IV coating plates, while the purified cells from C57 mice preferred type collagen-I dishes for their growth. Both cells displayed anti-PECAM-1 (CD31) and von Willebrand Factor immune-reactivity. These results indicated that different coating materials not only improve attachment of isolated cells but also promoting growth of cells, suggesting that this method of purifying murine Brain microvascular endothelial cells (BMEC) provides a suitable model to investigate blood-brain-barrier (BBB) properties within neurovascular unit in vitro.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2006.08a
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• pp.48-48
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• 2006

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.21 no.4
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• pp.96-103
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• 2007

In this paper, we present a novel and numerically efficient algorithm for high resolution TOA(Time Of Arrival) estimation under indoor radio propagation channels. The proposed algorithm is not dependent on the structure of receivers, i.e, it can be used with either coherent or non-coherent receivers. The TOA estimation algorithm is based on a high resolution frequency estimation algorithm of Minimum-norm. The efficiency of the proposed algorithm relies on numerical analysis techniques in computing signal or noise subspaces. The algorithm is based on the two step procedures, one for transforming input data to frequency domain data and the other for estimating the unknown TOA using the proposed efficient algorithm. The efficiency in number of operations over other algorithms is presented. The performance of the proposed algorithm is investigated by means of computer simulations.. Throughout the analytic and computer simulation results, we show that the proposed algorithm exhibits superior performance in estimating TOA estimation with limited computational cost.

• Nuclear Engineering and Technology
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• v.29 no.3
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• pp.181-185
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• 1997

The ion sources for induction linac driven heavy ion fusion were fabricated and their omittance characteristics were investigated. For to kinds of ion sources, i. e. a carbon vacuum arc ion source and a cusp field rf ion source, the emittance was measured with a double slit beam scanner. The required normalized omittance of an ion source for heavy ion fusion is 10$^{-7}$ - 5$\times$10$^{-7}$ $\pi$ m-rod, and the measured emittances of the ion beams from carbon vacuum arc ion source and cusp field rf ion source (Ne$^{+}$) were 2$\times$10$^{-6}$ $\pi$ m-rad and 4$\times$10$^{-7}$ $\pi$ m-rad, respectively.y.

• Animal cells and systems
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• v.10 no.2
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• pp.79-83
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• 2006

Tissue plasminogen activator (tPA) is used to lyse clots and reperfuse brain in ischemic stroke. However, sideeffects of intracerebral hemorrhage (ICH) and edema limit their clinical application. In part, these phenomena has been linked with elevations in matrix metalloproteinase-9 (MMP-9) in neurovascular unit. However little is known about their regulatory signaling pathways in brain cells. Here, I examine the role of MAP kinase pathways in tPA-induced MMP-9 regulation in rat cortical astrocytes. tPA $(1-10\;{\mu}g/ml)$ induced dose-dependent elevations in MMP-9 and MMP-2 in conditioned media. Although tPA increased phosphorylation in two MAP kinases (ERK, JNK), only inhibition of the JNK pathway by the JNK inhibitor SP600126 significantly reduced MMP-9 upregulation. Neither ERK inhibition with U0126 nor p38 inhibition with SB203580 had any significant effects. Taken together, these results suggest that c-jun N-terminal kinase (JNK) plays an essential role for tPA-induced MMP-9 upregulation.

• Animal Systematics, Evolution and Diversity
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• v.13 no.2
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• pp.173-192
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• 1997

Three damselfishes, Dascyllus melanurus, Dascyllus trimaculatus and Poamcentrus nagasakiensis were collected in the coastal waters of Cheju Island, Korea, during the period from July 1994 to May 1997. These taxa were a new records to th Korean fish fauna. This study re-describe about family Pomacentridae (6 genera, 13 species) including 3 new recorded species and suggest the taxonomic key of family pomacentridae from Korea.

• Korean Journal of Veterinary Research
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• v.9 no.2
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• pp.37-42
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• 1969

A survey on the Eperythrozoon infection of cattle was conducted by means of examinations of Giemsa staining blood films obtained from 2,263 cattle which had been fed in the middle and south parts of Korea. On the other hand, continuous hematological examinations to five splenectomized calves were performed. The results obtained were summarized as follows: 1. Eperythrozoon wenyoni were detected in the blood films of five cattle from Kyungpook and Cheju areas. 2. The infections of Eperythrozoon wenyoni were observed in four of five splenectomized calves from Taegu and Sungwhan areas. 3. From these results it is considered that the infections of Eperythrozoon wenyoni in cattle are quite common in the middle and south parts of Korea.

• Journal of the Korean Society for Precision Engineering
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• v.1 no.3
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• pp.95-105
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• 1984

The purpose of this paper is to predict the cutting force, utilizing new model of double cutting edge which has normal rake angle and tool inclination angle. Changing side, back rake angle and side cutting edge angle in the new model. Three dimensional cutting force was obtained by the use of .eta. /c=i proposed by Stabler and energy method for three dimen- sional cutting force. Theoretical results has been calculated with development of optimization algorism which can be put into three dimensional theory, using the method of least square with orthogonal cutting data. IT is proved that three dimensional cutting force is to be predicted accurately only if orthogonal cutting force by equalizing theoretical result and experimental result has been calculated.

• Proceedings of the Korean Biophysical Society Conference
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• 2003.06a
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• pp.29-29
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• 2003

Compartmentation of intracellular signaling pathways serves as an important mechanism conferring the specificity of G protein-coupled receptor (GPCR) signaling. In the heart, stimulation of $\beta$$_2$-adrenoceptor ($\beta$$_2$-AR), a prototypical GPCR, activates a tightly localized protein kinase A (PKA) signaling, which regulates substrates at cell surface membranes, bypassing cytosolic target proteins (eg, phospholamban). Although a concurrent activation of $\beta$$_2$-AR-coupled $G_{i}$ proteins has been implicated in the functional compartmentation of PKA signaling, the exact mechanism underlying the restriction of the $\beta$$_2$-AR-PKA pathway remains unclear. In the present study, we demonstrate that phosphatidylinositol 3-kinase (PI3K) plays an essential role in confining the $\beta$$_2$-AR-PKA signaling. Inhibition of PI3K with LY294002 or wortmannin enables $\beta$$_2$-AR-PKA signaling to reach intracellular substrates, as manifested by a robust increase in phosphorylation of phospholamban, and markedly enhances the receptor-mediated positive contractile and relaxant responses in cardiac myocytes. These potentiating effects of PI3K inhibitors are not accompanied by an increase in $\beta$$_2$-AR-induced cAMP formation. Blocking $G_{i}$ or $G_{$\square$$\square$}$ signaling with pertussis toxin or $\beta$ARK-ct, a peptide inhibitor of $G_{$\square$$\square$}$, completely prevents the potentiating effects induced by PI3K inhibition, indicating that the pathway responsible for the functional compartmentation of $\beta$$_2$-AR-PKA siglaling sequentially involves $G_{i}$, $G_{$\square$$\square$}$, and PI3K. Thus, PI3K constitutes a key downstream event of $\beta$$_2$-AR- $G_{i}$ signaling, which confines and negates the concurrent $\beta$$_2$-AR/Gs-mediated PKA signaling.gnaling.

• Journal of fish pathology
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• v.4 no.2
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• pp.71-77
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• 1991

From August to October of 1991, a bacterial disease occurred in cultured flounder, of 0 age Paralichthys olivaceus, in Cheju-do. Typical symptoms of the diseased fish were the hemorrage of the opercle, discoloration of the body, exophthalmus and hemorrhage of the eyes. The causative organism isolated from diseased fish was identified as a $\beta$-hemolytic Streptococcus. This bacterium was not identical with any known Streptococcus species listed in Bergey's Manual of Determinative Bacteriology 8th edition. However there was a close similarity between this species and Streptococcus sp. isolated from flounder and reported by Tashio (1983). The isolated bacteria streptococcus sp. showed very high sensitivity to the SM and EM, however, it resisted to the high concentration of Penicillin. In the pathagenicity analysis, about $1.1{\times}10^9$, $1.1{\times}10^7$, $1.1{\times}10^5$, $1.1{\times}10^3$, $1.1{\times}10^1cells$/100g B.W of isolated bacteria were injected percutaneouly. The results showed that this inoculation size $1.1{\times}10^3cells$/100g B.W was the threshold for the induction of mortality in pathogenicity analysis.