• 제목/요약/키워드: Cheju I

검색결과 345건 처리시간 0.024초

Primary Culture of Endothelial Cells from Murine Brain Microvessels

  • 이선령
    • 대한의생명과학회지
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    • 제12권2호
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    • pp.127-130
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    • 2006
  • It is important to coordinated interaction among neurons, astrocytes and endothelial cells to maintain the function of brain. To study their regulatory mechanisms in vitro system, the co-culture system among the isolated cells from brain may be needed. However, the method for purifying brain microvascular endothelial cells (BMEC) far culture have not established yet. In this study, the proper culture methods of mice cells using two different strains, CD1 and C57BL6, to obtain the pure and plentiful endothelial cells were described. The flatted-round forms of CD1 endothelial cells grew on the collagen-IV coating plates, while the purified cells from C57 mice preferred type collagen-I dishes for their growth. Both cells displayed anti-PECAM-1 (CD31) and von Willebrand Factor immune-reactivity. These results indicated that different coating materials not only improve attachment of isolated cells but also promoting growth of cells, suggesting that this method of purifying murine Brain microvascular endothelial cells (BMEC) provides a suitable model to investigate blood-brain-barrier (BBB) properties within neurovascular unit in vitro.

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A Study on High Resolution Ranging Algorithm for The UWB Indoor Channel

  • Lee, Chong-Hyun
    • 조명전기설비학회논문지
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    • 제21권4호
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    • pp.96-103
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    • 2007
  • In this paper, we present a novel and numerically efficient algorithm for high resolution TOA(Time Of Arrival) estimation under indoor radio propagation channels. The proposed algorithm is not dependent on the structure of receivers, i.e, it can be used with either coherent or non-coherent receivers. The TOA estimation algorithm is based on a high resolution frequency estimation algorithm of Minimum-norm. The efficiency of the proposed algorithm relies on numerical analysis techniques in computing signal or noise subspaces. The algorithm is based on the two step procedures, one for transforming input data to frequency domain data and the other for estimating the unknown TOA using the proposed efficient algorithm. The efficiency in number of operations over other algorithms is presented. The performance of the proposed algorithm is investigated by means of computer simulations.. Throughout the analytic and computer simulation results, we show that the proposed algorithm exhibits superior performance in estimating TOA estimation with limited computational cost.

Emittance Measurements of the Ion Sources for Induction Linac Driven Heavy Ion Fusion

  • Lee, Heon-Ju
    • Nuclear Engineering and Technology
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    • 제29권3호
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    • pp.181-185
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    • 1997
  • The ion sources for induction linac driven heavy ion fusion were fabricated and their omittance characteristics were investigated. For to kinds of ion sources, i. e. a carbon vacuum arc ion source and a cusp field rf ion source, the emittance was measured with a double slit beam scanner. The required normalized omittance of an ion source for heavy ion fusion is 10$^{-7}$ - 5$\times$10$^{-7}$ $\pi$ m-rod, and the measured emittances of the ion beams from carbon vacuum arc ion source and cusp field rf ion source (Ne$^{+}$) were 2$\times$10$^{-6}$ $\pi$ m-rad and 4$\times$10$^{-7}$ $\pi$ m-rad, respectively.y.

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Essential Role for c-jun N-terminal Kinase on tPA-induced Matrix Metalloproteinase-9 Regulation in Rat Astrocytes

  • Lee, Sun-Ryung
    • Animal cells and systems
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    • 제10권2호
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    • pp.79-83
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    • 2006
  • Tissue plasminogen activator (tPA) is used to lyse clots and reperfuse brain in ischemic stroke. However, sideeffects of intracerebral hemorrhage (ICH) and edema limit their clinical application. In part, these phenomena has been linked with elevations in matrix metalloproteinase-9 (MMP-9) in neurovascular unit. However little is known about their regulatory signaling pathways in brain cells. Here, I examine the role of MAP kinase pathways in tPA-induced MMP-9 regulation in rat cortical astrocytes. tPA $(1-10\;{\mu}g/ml)$ induced dose-dependent elevations in MMP-9 and MMP-2 in conditioned media. Although tPA increased phosphorylation in two MAP kinases (ERK, JNK), only inhibition of the JNK pathway by the JNK inhibitor SP600126 significantly reduced MMP-9 upregulation. Neither ERK inhibition with U0126 nor p38 inhibition with SB203580 had any significant effects. Taken together, these results suggest that c-jun N-terminal kinase (JNK) plays an essential role for tPA-induced MMP-9 upregulation.

자리돔과 (Family Pomacentridae) 어류의 형태학적 연구 1. 자리돔과 어류의 분류학 적 재검토 (Morphological Study on the Fishes of the Family Pomacentridae I. A Taxonomical Revision of the Family Pomacentridae (Pisces; Perciformes) from Korea)

  • 고정락;명정구;김용억
    • Animal Systematics, Evolution and Diversity
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    • 제13권2호
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    • pp.173-192
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    • 1997
  • 1994년 7월부터 1997년 5월사이 부산, 충무 및 제주도에서 채집된 자리돔과 어류가운데 Dascyllus melanurus(신칭:줄셋돔), Dascyllus trimaculatus(신칭:세점자리돔) 및 Pomacentrus nagasakiensis(신칭:나가사끼자리돔)가 국내 미기록종으로 분류되었다. 이들 3종의 계수, 계측형질을 기재하였으며 이들과 함께 자리돔과 어류 6속 13종에 대한 검색표를 제시하였다.

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우리나라 축우(畜牛)의 Eperythrozoon 감염(感染)에 관(關)한 연구(硏究) I. Eperythrozoon 감염(感染)의 실태조사(實態調査) (Studies on the Eperythrozoon infection of cattle in Korea 1. Survey on the Eperythrozoon infection)

  • 이학철;손제영;유동열;정영건
    • 대한수의학회지
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    • 제9권2호
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    • pp.37-42
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    • 1969
  • A survey on the Eperythrozoon infection of cattle was conducted by means of examinations of Giemsa staining blood films obtained from 2,263 cattle which had been fed in the middle and south parts of Korea. On the other hand, continuous hematological examinations to five splenectomized calves were performed. The results obtained were summarized as follows: 1. Eperythrozoon wenyoni were detected in the blood films of five cattle from Kyungpook and Cheju areas. 2. The infections of Eperythrozoon wenyoni were observed in four of five splenectomized calves from Taegu and Sungwhan areas. 3. From these results it is considered that the infections of Eperythrozoon wenyoni in cattle are quite common in the middle and south parts of Korea.

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에너지 방법을 이용한 삼차원 절삭력의 이론적 여측에 관한 연구 (A study on the Theoretical of Three Dimensional Cutting Force Used Energy Method)

  • 김장형
    • 한국정밀공학회지
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    • 제1권3호
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    • pp.95-105
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    • 1984
  • The purpose of this paper is to predict the cutting force, utilizing new model of double cutting edge which has normal rake angle and tool inclination angle. Changing side, back rake angle and side cutting edge angle in the new model. Three dimensional cutting force was obtained by the use of .eta. /c=i proposed by Stabler and energy method for three dimen- sional cutting force. Theoretical results has been calculated with development of optimization algorism which can be put into three dimensional theory, using the method of least square with orthogonal cutting data. IT is proved that three dimensional cutting force is to be predicted accurately only if orthogonal cutting force by equalizing theoretical result and experimental result has been calculated.

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양식(養殖) 넙치의 연쇄구균증(連鎖球菌症) (A streptococcal Disease of Cultured Flounder, Paralichthys olivaceus)

  • 이창훈;하동수
    • 한국어병학회지
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    • 제4권2호
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    • pp.71-77
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    • 1991
  • 1. 1991년(年) 8월(月)부터 10일(日)까지 제주도내(濟州道內) 넙치양식장 4개소(個所)의 병어에서 연쇄구균을 분리하였다. 2. 분리균(分離菌)은 $10^{\circ}C$, $45^{\circ}C$, 6.5% Nacl, pH 9.6 및 0.1% methylene blue milk 에서는 발육(發育)되지 않았고 또한 Indole 및 $H_2S$도 생성하지 못하였으며 $\beta$ 용혈성(溶血性)을 나타내었다. 3. 이상(以上)의 성상(性狀)에서 분리균(分離菌)은 Bergey's Manual of Determinative Bacteriology 및 Bergey's Manual os Systematic Bacteriology I 및 II에 근거(根據)를 두어 검색(檢索)한 결과(結果) Streptococcus pyogenes 및 Streptococcus epuisimilis와 유사하지만 약간의 차이(差異)가 있으므로 Streptococcus sp.로 하였다. 4. 분리균(分離菌)의 약제(藥劑) 감수성(感受性) 시험결과(試驗結果) Spiramycin 및 Erythromycin에 높은 감수성(感受性)을 나타냈으나 Penicillin에는 내성(耐性)을 나타냈다. 5. 분리균(分離菌)의 병원성(病原性) 시험결과(試驗結果) $1.1{\times}10^3cells$/100g 어체중 이상(以上) 접종(接種)하였을 때는 폐사(斃死) 미수(尾數)가 발생(發生)하였으나 그 이하(以下)의 접종(接種) 농도(濃度)에서는 폐사(斃死)가 발생(發生)하지 않았다.

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제주도 범섬의 식생구조 (The Vegetation Structure of Beomseom Islet, Jeju-do)

  • 김찬수;송관필;문명옥;강영제;변광옥;김문홍
    • 한국농림기상학회지
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    • 제7권3호
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    • pp.201-210
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    • 2005
  • 본 연구는 이 지역의 식생을 밝힘으로서 체계적이고 효율적인 보존관리대책을 마련하는데 필요한 정보를 제공하고, 금후의 식생변화과정을 연구하는데 필요한 기초 자료로 활용하고자 실시하였다. 범섬의 식생에 대한 군락을 분류한 결과 곰솔군락, 상록활엽수군락, 관목군락, 이대군락, 참억새군락, 암극식생의 여섯 가지 유형으로 구분되었다. 각 군락의 분포면적은 관목군락 40,230 $m^2$($23.3\%$), 암극식생이 39,366 $m^2$($22.8\%$), 곰솔군락 30,012$m^2$($17.4\%$), 참억새군락 29,853$m^2$($17.3\%$), 상록활엽수군락 5,564$m^2$($3.2\%$), 이대군락 3,325 $m^2$($1.9\%$)의 순으로 나타났다. 또한 식생이 형성되지 않은 지역인 해안단애지역은 24,246$m^2$($14.1\%$) 였다. 이러한 식생의 분포는 사면의 경사, 얕은 토양층 등 여러 가지 환경적인 입지요인과 최근까지 거주 및 농경이 이루어졌던 지역이므로 생태학적 간섭과 교란 때문인 것으로 추정된다.