• Journal of Microbiology and Biotechnology
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• v.31 no.12
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• pp.1722-1731
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• 2021

The genus Streptomyces is intensively studied due to its excellent ability to produce secondary metabolites with diverse bioactivities. In particular, adequate precursors of secondary metabolites as well as sophisticated post modification systems make some high-yield industrial strains of Streptomyces the promising chassis for the heterologous production of natural products. However, lack of efficient genetic tools for the manipulation of industrial strains, especially the episomal vector independent tools suitable for large DNA fragment deletion, makes it difficult to remold the metabolic pathways and streamline the genomes in these strains. In this respect, we developed an efficient deletion system independent of the episomal vector for large DNA fragment deletion. Based on this system, four large segments of DNA, ranging in length from 10 kb to 200 kb, were knocked out successfully from three industrial Streptomyces strains without any marker left. Notably, compared to the classical deletion system used in Streptomyces, this deletion system takes about 25% less time in our cases. This work provides a very effective tool for further genetic engineering of the industrial Streptomyces.

• Journal of Biosystems Engineering
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• v.30 no.1 s.108
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• pp.1-7
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• 2005

When a slope tractor works on the slope land, it travels usually along the contour and slope line. In that case, the efficiency of work generally decreases and the safety of the operator caused by the overturn of the tractor should be considered. Maintaining the tractor body being horizontal during the travel is crucial to solve problems. To overcome such a problem, an automatic leveling control system for slope tractor has been developed. The system composed of sensor for measuring rolling and pitching inclination of the slope tractor chassis, controller, hydraulic control system and mechanism. The limit angle of the leveling control was set up to be ${\pm}15^{\circ}C$ for rolling, ${\pm}7^{\circ}C$ for pitching. The proposed control and hydraulic power system was implemented to the prototype slope tractor. This paper shows results about development of the automatic leveling mechanism and response properties for slope tractor.

• Transactions of the Korean Society of Automotive Engineers
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• v.20 no.1
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• pp.1-7
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• 2012

The brake-by-wire technology is a new automotive chassis system that allows standard braking operations by electronic components with lighter weights and faster response. The brake-by-wire units such as EMB (Electro-Mechanical Brake) are controlled by electronic sensors and actuators and, thus, the fault diagnosis is essential for implementation. In this study, a model-based fault diagnosis system is developed for the sensors based on the analytical redundancy method. The fault detection algorithm is verified in simulations for various faulty cases. A test bench is built including the EMB unit and the performance of the proposed fault diagnosis system is evaluated through the experiment.

• Transactions of the Korean Society of Automotive Engineers
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• v.20 no.1
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• pp.8-13
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• 2012

In this paper, Hybrid Electric Vehicle is directly designed and manufactured for base study of HEV's system and Green Car. Foundation design consists of power train design and the frame design. The power train concept includes motor, engine, generator and battery. And the concept of the frame is the single-seat of this self-made HEV. A frame installed in hybrid system contains suspension, steering wheel, seat, accelerating pedal, brake pedal, clutch handle and various chassis parts with bearings. Electromagnetic clutch is equipped to transmit engine power to drive axle. The control algorism make using LabVIEW to control of an engine and a motor depending on drive condition. A parallel type hybrid system is manufactured to control operation of a motor and an engine depending on vehicle speed.

• ETRI Journal
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• v.41 no.5
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• pp.670-683
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• 2019

In this paper, we analyzed the signal integrity of a system interconnection module for a proposed high-density server. The proposed server integrates several components into a chassis. Therefore, the proposed server can access multiple computing resources. To support the system interconnection, among the highly integrated computing resources, the interconnection module, which is based on Serial RapidIO, has been newly adopted and supports a bandwidth of 800 Gbps while routing 160 differential signal traces. The module was designed for two different stack-up types on a printed circuit board. Each module was designed into 12- (version 1) and 14-layer (version 2) versions with thicknesses of 1.5T and 1.8T, respectively. Version 1 has a structure with two consecutive high-speed signal-layers in the middle of two power planes, whereas Version 2 has a single high-speed signal placed only in the space between two power planes. To analyze the signal integrity of the module, we probed the S-parameters, eye-diagrams, and crosstalk voltages. The results show that the high-speed signal integrity of Version 2 has a better quality than Version 1, even if the signal trace length is increased.

• IEMEK Journal of Embedded Systems and Applications
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• v.8 no.2
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• pp.69-78
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• 2013

As vehicles become more intelligent for safety and convenience of drivers, in-vehicle networking systems such as controller are network (CAN) have been widely used due to increasing number of electronic control unit (ECU). Recently, FlexRay was developed to replace CAN protocol in chassis networking systems, to remedy the shortage of transmission capacity and unsatisfactory real-time transmission delay of conventional CAN. However, it is difficult for vehicle network designers to calculate platform configuration registers (PCR) and determine a base cycle or slot length of FlexRay. To assist vehicle network designers for designing FlexRay cluster, this paper presents automatic field bus exchange format (FIBEX) generation algorithm from CANdb information, which is de-facto standard database format for CAN. To design this program, structures of FIBEX, CANdb and relationship among PCR variables are analyzed.

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2001.10a
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• pp.91-96
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• 2001

When it is considered that many vehicle rides on the road and ride quality is an important method to evaluate vehicle performance with handling, running-over-bump manoeuvre may be suitable for testing ride quality. In this paper, a computed model has roughly steering system and lumped mass, connected by joint each rigid body, and suspension that has beam elements and has shock absorber as force element to represent nonlinear characteristics. A computer simulations for passing over a bump were made with two velocities. One side of vehicle passed over bump in due consideration of driver's habit that driver is subject to avoid a bad ride quality. On simulation, vertical acceleration, pitch angle and roll angle were measured at the mass center of chassis each case.

• Journal of Korean Society for Atmospheric Environment
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• v.15 no.2
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• pp.175-182
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• 1999

Scanning electron microscopy (SEM) has played an important role in receptor modeling area because it is a powerful tool for characterizing individual particles. The purpose of the study was to classify individual diesel particles base on statistical analysis and image analysis by SEM/EDX (energy dispersive x-ray analyser). The diesel particles were sampled by both a modified CVS 75 mode and a high speed mode with a chassis dynamometer. The SEM/EDX system provides various physical parameters including particle's particle diameter and chemical information. Thus density and mass of the diesel particle were estimated cased on its chemical composition and further fractal dimensions of the diesel particle were obtained by the Hurst exponent method. The fractal dimension in the sample of modified CVS 75 mode was higher than the high speed mode. Finally, mass fractions for a diesel vehicle as a source profile were estimated cased on a particle class concept.

• Proceedings of the KIEE Conference
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• 1999.07f
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• pp.2502-2504
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• 1999

In $CO_2$ arc welding, which is generally used for steel Panel of chassis parts, spatters cause many troubles. This paper represents the Pulse MAG arc welding to limit spatter generation, which is controlled output current instantaneously. And the system designed in this Paper is the digital controller using DSP.

• Transactions of the Korean Society of Mechanical Engineers
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• v.12 no.3
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• pp.450-456
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• 1988

Dynamic analysis of a vehicle is carried out with rigid body and flexible body models. The chassis of the vehicle is treated as flexible body in the flexible body model, and vibration normal modes are considered to account for elastic deformation of the component. Using output from the modal analysis in the finite element program, input data for the dynamic analysis with flexible body is generated. To achieve the computational efficiency, SUPERELEMENT technique is used for the vehicle suspension subsisted. The computer simulation time with suspension superelement was much reduced due to the reduction of coordinates and no kinematic constraint in the system.