• Journal of Mushroom
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• v.18 no.4
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• pp.297-302
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• 2020

Five varieties of mushrooms, including Sae-do, were examined using farm field tests to determine their morphological characteristics and quality by performing harvest flush. Considering the cultivation characteristics of the varieties studied, the parts related to mushroom generation and production showed almost similar characteristics. Overall, the mycelial forces were similar; however, in the evaluation of plantation tests performed by farmers, "saedo" was found to be the strongest and "saehan" was considered to be the weakest. Weights of fruiting bodies were related more for compost status and regimen of use than for breeding, and it was difficult to distinguish between the weights of fruiting bodies of the varieties studied. Regardless of the variety, pileus diameter, pileus thickness, and stem length of Dalseong-gun, Yongin-si, and Boryeong-si decreased as the harvest flush progressed. However, stem thickness tended to increase in the second and third flush compared to that in the first flush, regardless of the variety. However, the Gyeongju-si farms could not identify any trends attributable to varieties and harvest flushes. The pileus size and stem length of the fruiting bodies of varieties from Buyeo farms decreased with the progression of the harvest flush. However, pileus thickness and stem thickness did not show any specific tendency of growth among the varieties studied. The differences in the morphological characteristics of mushroom varieties in each harvest cycle are expected to be caused by differences in the supply of nutrients in the medium, the quality of the compost used, and the cultivation environment rather than the variety.

• Journal of Mushroom
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• v.4 no.3
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• pp.116-121
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• 2006

'Seoln', a new variety of oyster mushroom was developed by Mushroom Research Institute, Gyonggi Province Agricultural Research and Extension Services in 2005. It was bred with mating between monokaryotic strains isolated from KME35124 and $F{\cdot}X3$. The major characteristics of the mushroom was shown a lot of primordia, the gray-colored and bunch pileus. The optimum temperature for the mycelial growth was around $26{\sim}30^{\circ}C$ and that for the primordia and growth of fruit-body was around $10{\sim}15^{\circ}C$. Incubation period was required around 25 days with bottle culture and about 23 days in Poly Prophylene(P.P) plastic bag culture. The yield was shown high by 138.8g/bottle and 250.9%/P.P bag.

• The Korean Journal of Mycology
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• v.37 no.1
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• pp.19-27
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• 2009

This study was conducted to investigate genetic characteristics of 25 Pleurotus eringii strains that have been released in Korea based on cultural, morphological features and PCR fingerprints. Strains PER-007 and PER-012 showed distinct cultural characteristics in growth rate, morphological characteristics of mycelial colony and fruiting bodies when compared to those of other strains. Strain PER-007 did not form primordium initiation in sawdust medium and PER-012 also showed different phenotypes on fruiting bodies. Eleven URP primers were used to detect PCR polymophic bands in P. eringii strains. Primers URP1F, URP2R, URP2F, URP4R, URP6R, URP9F and URP17R were selected as useful primers for amplifying PCR polymorphic bands in P. eringii strains. The genetic similarity index was calculated by using PCR polymorphic bands amplified by eight URP primers among the 25 strains. The P. eringii strains were grouped by four distinct clusters on the UPGMA analysis. The genetic similarity values ranged from 100% to 76% were observed in three major groups, suggesting close genetic relatedness of them. Exceptionally, PER-007 and PER-017 were involved in outgroup.

• The Korean Journal of Mycology
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• v.51 no.2
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• pp.69-80
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• 2023

The cultivation conditions of shiitake mushrooms (Lentinula edodes) influence the production and quality of fruiting bodies. We conducted this study to improve the productivity and quality of shiitake mushrooms by modifying the cultivation conditions. Two types of spawns (sawdust and liquid spawn) were used, and corn flour was used as a nutritional source for the sawdust medium. A blue light-emitting diode (LED; 300 lux) was also used instead of a white LED during the incubation period. Sanbaekhyang was used as the experimental variety. When using corn flour, the mycelial growth rate increased 1.1 to 2.7 times the growth rate of the control up to 21 days of incubation, and the weight loss rate of the media was also higher. Mushroom productivity increased 1.2 times when the liquid spawn was used compared to when the sawdust spawn was used, and the blue LED also increased fruiting body production by 1.1 times compared to the white LED. Mushroom productivity increased when the liquid spawn was used, and the blue LED also increased fruiting body production. Fruiting body weight and the size of the cap were greater when sawdust spawn was used. The fruiting body weight and the stipe diameter were greater when the blue LED was used. Taste analysis showed that the saltiness increased when corn flour was used, and the sourness increased when the blue LED was used.

• Journal of Mushroom
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• v.6 no.3_4
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• pp.115-120
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• 2008

A new commercial strain 'Garlmoe' was developed by crossing monokaryons from selected wild strains. The optimal temperature of mycelial growth was $25^{\circ}C$ but it needed to adjust to $16{\sim}18^{\circ}C$ when incubated at the bottle cultivation. The optimal temperatures of fruiting body initiation and development were almost same to others like as $14^{\circ}C$ and $7{\sim}10^{\circ}C$, respectively. The color of 'Garlmoe' was typical to wild winter mushroom, that is, pileus was brown and lower part of stipe was dark brown. The surface on pileus of 'Garlmoe' was sticky 'Garlmoe' was a good commercial strain with high quality and high productivity characterized as quite even budding habit, straight and strong stipes and convex type pilei. The taste of this variety was very excellent because of better chewing feeling than white ones.

• Research in Plant Disease
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• v.19 no.2
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• pp.118-120
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• 2013

During the early spring of 2007 to 2009, stem rot of sweet potato (Ipomoea batatas L.) caused by Sclerotium rolfsii occurred in seedling stage grown in plastic film house at Iksan. In seedling stage of sweet potato, symptoms were initially appeared in yellowing and then the seedlings were eventually wilted. The fungus produced abundant white silky mycelium on infected tissues and soil line. Seedlings were very susceptible and died quickly once they were infected. The whole area of a petridish was rapidly covered with white mycelium on agar medium. Sclerotia began to produce after 7 days of mycelial growth and white sclerotia quickly melanized to a dark brown coloration. The causal agent isolated from the diseased plants was identified as Sclerotium rolfsii Saccardo on the basis of the morphological and cultural characteristics. All isolates of S. rolfsii caused similar symptoms on the host petioles by artificial inoculation.

• Research in Plant Disease
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• v.12 no.1
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• pp.55-57
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• 2006

Stem rot of Houttuynia cordata occurred in the experiment field of Research Institute of Natural Product, Uiseong, Gyeongbuk, Korea in July 2003. The typical symptoms of the disease were water-soaking, stem rot, necrotic tuber. White mycelial mats were spread over lesions and sclerotia were formed on died plant and near soil line. The sclerotia were spherical and irregular, 1-2.5 mm on potato dextrose agar (PDA), white to brown in color, The optimal temperature for hyphal growth was $28{\sim}30^{\circ}C$ and the hyphal diameter was $3{\sim}9{\mu}m$. Clamp connections were observed on PDA. Pathogenicity of the casual organism was provided on H. cordata according to Koch's postulate. The fungus was identified as Sclerotium rolfsii Saccardo on the basis of mycological characteristics and pathogenicity test to host plants. This is the first report on the stem rot of H. cordata caused by S. rolfsii in Korea.

• Research in Plant Disease
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• v.17 no.2
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• pp.225-227
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• 2011

The stem rot of Chrysanthemum zawadskii var. latilobum occurred in the landscape field of Sanggwan-myeon, Wanju-gun, Jeonbuk, Korea in September 2009. The typical symptoms of the disease were water-soaking and dark brown rot on the stem, wilting and blighting. Most infected plants died of the disease. White mycelial mats were spread over lesions and brown sclerotia were formed on stems and near soil surface. The sclerotia were spherical or irregular, 0.8-3.5 (mean 1.8) mm in size on potato dextrose agar, brown in color. The optimal temperature range of hyphal growth was $25-30^{\circ}C$ and the hyphal diameter was 4-9 ${\mu}m$. Clamp connections were observed in the hyphae of the fungus grown on potato dextrose agar. Pathogenicity of the casual organism was provided on C. zawadskii according to Koch's postulate. The fungus was identified as Sclerotium rolfsii on the basis of mycologiclal characteristics and pathogenicity test to host plants. This is the first report on the stem rot of C. zawadskii caused by S. rolfsii in Korea.

• Journal of Mushroom
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• v.12 no.2
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• pp.117-121
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• 2014

The experiments were conducted to provide information on the chemical concentrations and cultural characteristics in the periods of hyphal incubation, primordial formation, and fruiting bodies yield of winter mushroom, Flammulina velutipes at the mixture ratio of the raw materials. Substrates were analyzed for pH, total carbon (T-C), total nitrogen (T-N), and C/N ratio. In case of Flammulina velutipes, yield of fruiting bodies were 190.5 g/850 ml in the substrates, [Corn-cob + Rice bran + Soybean curd residue (75:20:5)], which was increased 20.4% more than the control, [Douglas fir sawdust + Rice bran (75:25)]. But the periods of hyphal incubation took 28 days, which was 7 days longer than the control. Also, in the substrates [Douglas fir sawdust + Rice bran + Soybean curd residue (75:20:5)], the yields was 172.7 g/850 ml and the periods of hyphal incubation was 21 days.

• The Korean Journal of Mycology
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• v.29 no.2
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• pp.131-133
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• 2001

A destructive collar rot disease was found on broad bean (Vicia faba) in several farmer's field located in Changseon-myon, Namhae-gun, Gyeongsangnam-do in 2001. The typical symptoms of the disease were stem rot, crown rot, wilt or blight. Upper parts of the infected stems were mostly blighted and white mycelial mats were spread over lesions and the sclerotia were formed on the stems near soil line. The infection rates of the disease in the surveyed area were ranged from 28.6 to 42.8%. The sclerotia of the fungus readily formed in artificial media such as PDA at $30^{\circ}C$ and its shape was globoid or irregular and size was $1.0{\sim}3.7{\times}1.0{\sim}2.8\;mm\;(av.\;1.0{\sim}2.3\;mm)$ and was brown or dark brown in color. The optimum temperature for growth of the fungus was about $30^{\circ}C$. The typical clamp connections were found in the hypha of the fungus grown on PDA. On the basis of mycological characteristics and pathogenecity test on host plants, the fungus was identified as Sclerotium rolfsii. This is the first report on the collar rot of broad bean caused by Sclerotium rolfsii in Korea.