• 대한물리의학회지
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• 제18권1호
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• pp.87-97
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• 2023

PURPOSE: The purpose of this study was to investigate the effect of multimodal intervention through VR (virtual reality)-based immersion program on the cognitive function and brain activity of patients with mild cognitive impairment. METHODS: The subjects of the study were 10 people in the experimental group who applied a complex intervention that performed cognitive tasks using the movement of the upper extremities through the VR program, and 10 people in the control group who received traditional occupational therapy. After the study intervention was applied 5 times a week, 30 minutes a day for a total of 8 weeks, LOTCA-G(Lowenstein Occupational Therapy Cognitive Assessment for Geriatric Population) and NIRSIT LITE were used to compare. RESULTS: Significant differences in cognitive function and brain activity were noted between the pre- test and post-test in the experimental group. Brain activity showed statistically significant differences in four channels of the working memory domain and one channel of the metacognitive domain (p < . 05). Comparative analysis of the difference between the two groups revealed statistically significant differences in cognitive function and brain activity. The brain activity showed statistically significant differences in three channels of the working memory domain and one channel of the metacognitive domain (p < .05). CONCLUSION: Through the results of this study, it was found that the complex intervention of performing cognitive tasks using upper extremity movements through the VR program had a positive effect on the cognitive function of patients with mild cognitive.

• Journal of Microbiology and Biotechnology
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• 제21권5호
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• pp.477-482
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• 2011

There are accumulating evidences suggesting that connexin (Cx), a gap junction channel-forming protein, acts as a growth suppressor in various cancer cells, and this effect is attributeed to the gap junction-mediated intercellular communication (GJIC). In order to characterize the relationship between the growth-arresting activity of Cx26 and its cytoplasmic localizations after expression, we linked a nuclear export signal (NES) sequence to Cx26 cDNA before transfecting into a rat breast cancer cell line. A confocal fluorescent microscopic observation revealed that the insertion of NES minimized the nuclear expression of Cx26, and increased its cytoplasmic expression, including plasma membrane junctions. Total cell counting and BrdUrd-labeling experiments showed that the growth of the breast cancer cells was inhibited by 74% upon transfection of Cx26-NES, whereas only 9% inhibition was observed with only Cx26 cDNA.

• Journal of Photoscience
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• 제3권3호
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• pp.133-136
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• 1996

Calcium modulates the activity of guanylate cyclase and plays a key role in dark and light adaptation in the visual system. We have measured the Ca$^{2+}$, K$^+$ and Na$^+$ concentration in dark and light adapted bullfrog's (Rana catesbeiana) vitreous humor by using the atomic absorption spectrophotometer. The calcium concentration of the light adapted bullfrog's vitreous humor was higher than that of the dark adapted bullfrog's vitreous humor. This means that ion activity between the photoreceptor and vitreous humor side is light dependent and we have found that a Ca$^{2+}$ channel and Na$^+$ - Ca$^{2+}$ exchanger exist in the vitreous humor.

• Bulletin of the Korean Chemical Society
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• 제31권9호
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• pp.2451-2452
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• 2010

• Korean Journal of Acupuncture
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• 제36권4호
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• pp.241-251
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• 2019

Objectives : The purpose of this study was to examine the effects of electroacupuncture at GB14, on brain activity assessed on an electroencephalogram(EEG). Methods : (1) Thirty-four healthy participants were randomly divided into two groups, receiving either real acupuncture or non-penetrating sham acupuncture at GB14. (2) EEG measurements were conducted simultaneously before and after a 5-minute electroacupuncture stimulation, and the differences in the resulting EEG parameters were compared between the test and control groups. Results : (1) Absolute power increased significantly in the theta-wave channel of the occipital region and in the entire alpha-wave channel. (2) Relative power decreased significantly in the theta-wave channels of the frontal and occipital regions. (3) Coherence decreased significantly in the theta- and beta-wave channels of the parietal and occipital regions, and increased significantly in the alpha-wave channels of most areas. Conclusions : This study shows that electroacupuncture stimulation at GB14 activates the alpha frequency band in particular.

• 한국음향학회지
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• 제33권2호
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• pp.139-145
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• 2014

본 논문에서는 WPD (Wavelet Packet Decomposition) 계수에 Teager 에너지를 적용한 특징 계수를 임계값 알고리듬에 적용하여 잡음에 강인한 VAD 알고리듬을 제안하였다. 임계값은 비음성 구간의 평균과 표준편차를 추산하여 설정하였다. TIMIT 음성과 NOISEX 잡음 데이터베이스를 사용한 실험 결과, 제안된 알고리듬이 기존의 대표적인 비교 대상 알고리듬보다 우수함을 보였다. 정확도는 SNR 10 dB부터 -10 dB까지 ROC (Receiver Operating Characteristics) 곡선을 사용하여 비교하였다.

• 디지털융복합연구
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• 제11권2호
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• pp.115-125
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• 2013

본 연구에서는 의류제품 온라인쇼핑 후 실패를 경험한 소비자가 쇼핑채널에서 제공하는 다양한 서비스 회복노력을 경험한 후 느끼게 되는 만족도에 대하여 알아보고, 또한 의류 소비자가 평소에 온라인 쇼핑에 대하여 지각하는 혜택과 위험이 서비스회복노력 경험 후 채널에 대한 만족도 평가에 어떠한 영향을 미치는가를 알아보고자 하였다. 서울 시내 남녀 대학생을 대상으로 온라인 쇼핑을 통해 의류제품을 구매할 때에 예상되는 서비스 주요 실패 유형과 온라인 쇼핑채널 운영자가 제공하는 서비스 회복유형별 만족도 그리고 채널경향에 대한 전반적인 관계를 조사하여 분석한 결과 의류제품의 온라인 쇼핑에서 나타날 수 있는 주요 서비스 실패 유형은 맞음새, 잘못된 제품정보, 제품결함, 재고부족, 느린 배송으로 나타났다. 소비자가 서비스 실패 유형에 따라 소비자가 만족하는 회복 유형에 다른 영향을 주는 것을 확인 할 수 있었다. 소비자는 서비스 실패를 경험하게 되면 기본적으로 채널전환의 욕구를 가지며 이와 같은 채널전환 경향은 서비스 회복노력에 대한 만족도가 낮을 때 더 강한 것으로 나타났다. 반면, 지각된 위험에 따른 고저 집단의 만족도 차이는 미비하게 나타났다.

• The Korean Journal of Physiology and Pharmacology
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• 제24권1호
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• pp.101-110
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• 2020

Transient receptor potential canonical 4 (TRPC4) channel is a nonselective calcium-permeable cation channels. In intestinal smooth muscle cells, TRPC4 currents contribute more than 80% to muscarinic cationic current (mIcat). With its inward-rectifying current-voltage relationship and high calcium permeability, TRPC4 channels permit calcium influx once the channel is opened by muscarinic receptor stimulation. Polyamines are known to inhibit nonselective cation channels that mediate the generation of mIcat. Moreover, it is reported that TRPC4 channels are blocked by the intracellular spermine through electrostatic interaction with glutamate residues (E728, E729). Here, we investigated the correlation between the magnitude of channel inactivation by spermine and the magnitude of channel conductance. We also found additional spermine binding sites in TRPC4. We evaluated channel activity with electrophysiological recordings and revalidated structural significance based on Cryo-EM structure, which was resolved recently. We found that there is no correlation between magnitude of inhibitory action of spermine and magnitude of maximum current of the channel. In intracellular region, TRPC4 attracts spermine at channel periphery by reducing access resistance, and acidic residues contribute to blocking action of intracellular spermine; channel periphery, E649; cytosolic space, D629, D649, and E687.

• The Korean Journal of Physiology and Pharmacology
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• 제16권1호
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• pp.25-30
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• 2012

Under some pathological conditions as bile flow obstruction or liver diseases with the enterohepatic circulation being disrupted, regurgitation of bile acids into the systemic circulation occurs and the plasma level of bile acids increases. Bile acids in circulation may affect the nervous system. We examined this possibility by studying the effects of bile acids on gating of neuronal (N)-type $Ca^{2+}$ channel that is essential for neurotransmitter release at synapses of the peripheral and central nervous system. N-type $Ca^{2+}$ channel currents were recorded from bullfrog sympathetic neuron under a cell-attached mode using 100 mM $Ba^{2+}$ as a charge carrier. Cholic acid (CA, $10^{-6}M$) that is relatively hydrophilic thus less cytotoxic was included in the pipette solution. CA suppressed the open probability of N-type $Ca^{2+}$ channel, which appeared to be due to an increase in (no activity) sweeps. For example, the proportion of sweep in the presence of CA was ~40% at +40 mV as compared with ~8% in the control recorded without CA. Other single channel properties including slope conductance, single channel current amplitude, open and shut times were not significantly affected by CA being present. The results suggest that CA could modulate N-type $Ca^{2+}$ channel gating at a concentration as low as $10^{-6}M$. Bile acids have been shown to activate nonselective cation conductance and depolarize the cell membrane. Under pathological conditions with increased circulating bile acids, CA suppression of N-type $Ca^{2+}$ channel function may be beneficial against overexcitation of the synapses.

• The Korean Journal of Physiology
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• 제25권1호
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• pp.17-26
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• 1991

Single smooth muscle cells of the rabbit pulmonary artery were isolated by treatment with collagenase and elastase. Using the patch clamp technique, potassium channel activity was recorded from the inside-out membrane patch. The channel had a sin히e channel conductance of about 360 pS in symmetrical concentration of K on both sides of the patch, 150 mM, and had a linear current-voltage relationship. During the application of 10 mM tetraethylammonium (TEA) to the intracellular membrane surface, the amplitude of single channel current was reduced and very rapid flickering appeared. The open probability $(P_0)$ of this channel was increased by increasing positivity of the potential across the patch membrane, with e-fold increase by 20 mV depolarization, and by increasing the internal $Ca^{2+}$ concentration. These findings are consistent with those of large conductance Ca-activated K channels reported in other tissues. But the shortening of the mean open time by increasing $[Ca^{2+}]_i$, was an unexpected result and one additional closed state which might be arisen from a block of the open channel by Ca binding was suggested. The $P_0-membrane$ potential relationship was modulated by internal pH. Decreasing pH reduced $P_0$. Increasing pH not only increased $P_0$ but also weakened the voltage dependency of the channel opening. The modulation of Ca-activated K channel by pH was thought to be related to the mechanism of regulation of vascular tone by the pH change.