The Korean Journal of Physiology
- Volume 25 Issue 1
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- Pages.17-26
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- 1991
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- 0300-4015(pISSN)
Effect of pH on Calcium-Activated Potassium Channels in Pulmonary Arterial Smooth Muscle Cells of the Rabbit
- Lee, Suk-Ho (Dankook University) ;
- Ho, Won-Kyung (Departments of Physiology, Seoul National University College of Medicine) ;
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Earm, Yung-E
(Departments of Physiology, Seoul National University College of Medicine)
- Published : 1991.06.01
Abstract
Single smooth muscle cells of the rabbit pulmonary artery were isolated by treatment with collagenase and elastase. Using the patch clamp technique, potassium channel activity was recorded from the inside-out membrane patch. The channel had a sin히e channel conductance of about 360 pS in symmetrical concentration of K on both sides of the patch, 150 mM, and had a linear current-voltage relationship. During the application of 10 mM tetraethylammonium (TEA) to the intracellular membrane surface, the amplitude of single channel current was reduced and very rapid flickering appeared. The open probability