• 동의생리병리학회지
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• 제18권4호
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• pp.1163-1168
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• 2004

In order to establish the experimental clinical model for evaluating the influences on the sexual hormones of SD-rat administrated with Methoxychlor(MET), it was dissolved in acetone and olive oil (1:19), which was administrated orally at doses of 200㎎/㎏ body weight/day for 7 days. The Testosterone value was measured in sera by Gamma Count with 1251 isotype, also weights of the body, testis and liver were measured for 15 weeks. Testosterone in the serum of intacted SD-rats was 0.51±0.43ng/㎖(n=5), but it was 0.69±0.18(n=2)ng/㎖l on 1 week. 0.28±0.05ng/㎖(n=2) on 3 week, 1.52±0.95ng/㎖(n=3) on 5 week, and 0.54±1.95 ng/㎖(n=3) 15 week post-dosing MET, respectively. Histologically, the numbers of spermatozoa and Leydig cells were reduced, which might influence to reduce sexual hormone, and AST and ALT was not increased in the serum due to administrating with MET, blood profile was not changed according to the administration of MET excepting MCH(Mean Corpuscular Hemoglobin). This study suggest that change of male sexual hormone by MET be a experimental model for evaluating some drugs associate with sex hormone.

• 대한약침학회지
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• 제17권1호
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• pp.20-26
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• 2014

Objectives: The study aimed to determine changes in laboratory data for cancer patients receiving Korean medicine (KM) care, with a focus on patients' functional status, cancer-coagulation factors and cancer immunity. Methods: We conducted an observational study of various cancer patients in all stages admitted to the East-West Cancer Center (EWCC), Dunsan Korean Hospital of Daejeon University, from Mar. 2011 to Aug. 2011. All patients were under the center's multi-modality Korean-medicine-based inpatient cancer care program. The hospitalization stay at EWCC ranged from 9 to 34 days. A total of 80 patients were followed in their routine hematologic laboratory screenings performed before and after hospitalization. Patients were divided into three groups depending on the status of their treatment: prevention of recurrence and metastasis group, KM treatment only group, and combination of conventional and KM treatment group. The lab reports included natural killer cell count (CD16 + CD56), fibrinogen, white blood cell (WBC), lymphocytes, monocytes, neutrophil, red blood cell (RBC), hemoglobin, platelet, Erythrocyte Sedimentation Rate (ESR), and Eastern Cooperative Oncology Group (ECOG) performance status. Results: With a Focus on patients' functional status, cancer-coagulation factors and cancer immunity, emphasis was placed on the NK cell count, fibrinogen count, and ECOG scores. Data generally revealed decreased fibrinogen count, fluctuating NK cell count and decreased ECOG, meaning improved performance status in all groups. The KM treatment only group showed the largest decrease in mean fibrinogen count and the largest increase in mean NK cell count. However, the group's ECOG score showed the smallest decrease, which may be due to the concentration of late-cancer-stage patients in that particular group. Conclusions: Multi-modality KM inpatient care may have positive effect on lowering the cancer coagulation factor fibrinogen, but its correlation with the change in the NK cell count is not clear.

• 대한영양사협회학술지
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• 제26권1호
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• pp.14-29
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• 2020

Dietary nitrate supplementation from green leafy vegetables and beetroot is involved in the 'nitrate-nitrite-nitric oxide (NO) pathway' and is reported to have important vascular impacts. This study evaluated the blood parameters and blood flow change subsequent to a raw juice diet program. The 28 subjects who voluntarily participated in the raw juice diet program at Sahmyook University were instructed to drink the juice composed of fruits and vegetables instead of partaking the usual meal, at a scheduled time daily. The study was approved by the research ethics council of the Sahmyook University (SYUIRB 2014-043). Evaluation of the participants after the required duration revealed decreased levels of soft lean mass (P<0.05), skeletal muscle mass (P<0.001), body fat mass (P<0.001), body mass index (P<0.001) and circulating levels of total cholesterol (P<0.001), triglycerides (P<0.05), HDL-cholesterol (P<0.01), LDL-cholesterol (P<0.01), and blood urea nitrogen (P<0.001). Conversely, circulating levels of creatinine (P<0.001), hemoglobin (P<0.001), and hematocrit (P<0.01) were increased after the intervention. The blood flow levels were also increased after the program (P<0.001). Our results indicate that a short-term raw juice diet contributes to the improvement of vascular blood flow and blood lipid parameters, and decreases the lean muscle mass related to dehydration.

• 한국수산과학회지
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• 제51권4호
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• pp.411-419
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• 2018

This study investigated the survival rates and physiological responses of Korean rockfish, Sebastes schlegeli, in live fish containers ($8^{\circ}C$, 33 psu) for 18 days. The survival rate was 99% and 97% in the control and experimental groups, respectively. Hematocrit and hemoglobin did not differ significantly between the control and experimental groups. Glucose and cortisol rose immediately on the first day of containment, but both gradually normalized. Aspartate aminotransferase and alanine aminotransferase did not differ significantly between the two groups after recovery. $NH_3$ continued to rise after the first day, but decreased to a level not significantly different from that of the control group during the recovery period. Plasma ions and osmolality did not change abnormally. The hemocyte population was not significantly different from that of the control. The ratios of apoptotic and necrotic cells indicated no specific variation in hemocyte viability. The histological changes in the skin and gills were not significantly different from those seen in the control. The experimental data obtained in this study suggest that live fish containers may be used to transport Korean rockfish without significantly affecting their physiology or survival.

• Journal of Gastric Cancer
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• 제15권4호
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• pp.262-269
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• 2015

Purpose: Intraabdominal abscess is one of the most common reasons for re-hospitalization after gastrectomy. This study aimed to develop a model for estimating the probability of intraabdominal abscesses that can be used during the postoperative period. Materials and Methods: We retrospectively reviewed the clinicopathological data of 1,564 patients who underwent gastrectomy for gastric cancer between 2010 and 2012. Twenty-six related markers were analyzed, and multivariate logistic regression analysis was used to develop the probability estimation model for intraabdominal abscess. Internal validation using a bootstrap approach was employed to correct for bias, and the model was then validated using an independent dataset comprising of patients who underwent gastrectomy between January 2008 and March 2010. Discrimination and calibration abilities were checked in both datasets. Results: The incidence of intraabdominal abscess in the development set was 7.80% (122/1,564). The surgical approach, operating time, pathologic N classification, body temperature, white blood cell count, C-reactive protein level, glucose level, and change in the hemoglobin level were significant predictors of intraabdominal abscess in the multivariate analysis. The probability estimation model that was developed on the basis of these results showed good discrimination and calibration abilities (concordance index=0.828, Hosmer-Lemeshow chi-statistic P=0.274). Finally, we combined both datasets to produce a nomogram that estimates the probability of intraabdominal abscess. Conclusions: This nomogram can be useful for identifying patients at a high risk of intraabdominal abscess. Patients at a high risk may benefit from further evaluation or treatment before discharge.

• 여성건강간호학회지
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• 제27권2호
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• pp.75-92
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• 2021

Purpose: This study aimed to analyze the content and effectiveness of psychosocial support interventions for women with gestational diabetes mellitus (GDM). Methods: The following databases were searched with no limitation of the time period: Ovid-MEDLINE, Cochrane Library, Ovid-Embase, CINAHL, PsycINFO, NDSL, KoreaMed, RISS, and KISS. Two investigators independently reviewed and selected articles according to the predefined inclusion/exclusion criteria. ROB 2.0 and the RoBANS 2.0 checklist were used to evaluate study quality. Results: Based on the 14 selected studies, psychosocial support interventions were provided for the purpose of (1) informational support (including GDM and diabetes mellitus information; how to manage diet, exercise, stress, blood glucose, and weight; postpartum management; and prevention of type 2 diabetes mellitus); (2) self-management motivation (setting goals for diet and exercise management, glucose monitoring, and enhancing positive health behaviors); (3) relaxation (practicing breathing and/or meditation); and (4) emotional support (sharing opinions and support). Psychosocial supportive interventions to women with GDM lead to behavioral change, mostly in the form of self-care behavior; they also reduce depression, anxiety and stress, and have an impact on improving self-efficacy. These interventions contribute to lowering physiological parameters such as fasting plasma glucose, glycated hemoglobin, and 2-hour postprandial glucose levels. Conclusion: Psychosocial supportive interventions can indeed positively affect self-care behaviors, lifestyle changes, and physiological parameters in women with GDM. Nurses can play a pivotal role in integrative management and can streamline the care for women with GDM during pregnancy and following birth, especially through psychosocial support interventions.

• 대한지역사회영양학회지
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• 제6권5호
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• pp.789-797
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• 2001

This study was conducted to find the differences in food consumption frequency of the middle aged(male 20, female 50) and the elderly(male 15, female 15) living in Chonju in December, 1998 according to food habits, smoking, alcohol drinking and exercising habit, health status, and the levels of nutrition knowledge and attitude store. The foods frequently consumed among the subjects were kimchi(15.4/week), mixed rice(11.5/week), rice(7.6/week), vegetables in soup, jjigae and jorim(5.0/week), mandarins and oranges(5.e/week), and seasoned laver(4.3/week). There were several factors influencing food consumption patterns. These were age, regularity of meal times, the status of smoking, alcohol drinking and exercising, and the level of nutrition attitude. The elderly ate cooked rice more frequently, while the middle aged ate fish, especially blue fishes more frequently. The middle aged who had breakfast regularly ate milk and milk products, legumes and fruits frequently. Those who smoked seemed to eat less cereals and starches and fats, while those who didn't smoke ate more sugars. The alcohol drinking group also ate less fats and the exorcising group ate almost all of food groups frequently. The status of health showed to be related with food consumption patterns. The normal group in hemoglobin ate eggs more frequently than the anemia group and the high risk group in blood pressure ate almost all of food groups more frequently. The high level group for nutrition attitude score chose vegetables, fruits, and milk and milk products more frequently than the other groups. On the other hand, low level group for nutrition attitude score was apt to eat ramyun, ham-sausage, and carbonated beverages more frequently. Therefore, nutrition education to improve the food habits find to change nutrition attitude is necessary to promote health status anti mole attention should be taken to the high risk group in blood pressure to guide proper food and nutrition intakes.

• 대한지역사회영양학회지
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• 제5권2호
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• pp.161-171
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• 2000

The purpose of this study was to investigate the effects of smoking and/or alcohol drinking on the nutrient intake, nutritional status, and eating habits of adult males. The subjects were 157 male adults aged 20-49 living in Daejon. Interviews with questionaire on smoking and alcohol drinking habits and eating habits, anthropometric measurement, biochemical blood test, and dietary assessment by one-day 24 hour recall and two-day diet record were carried out. The subjects were divided into four groups : control (non-smoking and non-drinking), drinking only, smoking only, and the both(drinking and smoking). The average numbers of smoked cigarettes were 17.8/d and 19.1/d in the smoking only group and the both group, respectively. The frequency of alcohol drinking was 8.3 times/month and 11.6 times/month in the drinking only group and the both group, respectively. Height was significantly higher(P 0.05) in the drinking only group than in the none and smoking only groups, while the other anthropometric indices were not different among the four groups. There were positive correlations between smoking and drinking or coffee intake. Alcohol drinking increased eating-out frequency and the intakes of energy, protein, dietary fiber, vitamin A, B$_1$,$ B_6,$ Fe and P, while smoking decreased snack frequency and intakes of energy, protein and vitamin $ B_6,$. Blood pressure was not changed in the smoking only and drinking only groups compared to the none group, while systolic blood pressure was elevated in both group(P 0.05). Alcohol drinking significantly elevated(P 0.05) serum total cholesterol and LDL-cholestrol level, while smoking did not change the serum lipid and cholesterol levels. Hemoglobin nd MCHC levels were significantly elevated(P 0.05) by smoking. From these data, it is suggested that both smoking and drinking have influence on some eating habits and nutrient intakes, and especially alcohol drinking can induce hypercholesterolemia.

• 대한지역사회영양학회지
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• 제5권2호
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• pp.185-192
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• 2000

As the percentage of the elderly in the population increases, research to identify the nutritional and health status of the elderly, to understand the factors influencing on them, and to delay aging have been done actively. It is necessary for these studies to be longitudinal. This study was carried out to assess the change of health-related habits such as dietary behaviors and smoking, alcohol drinking and exercising habit, and health status between previous study(1991/1994) and post study(1998) for middle-aged men and women living in Chonju. For the present study, the data of 63 pairs(23 men and 40 women) were analyzed. They were 11 men and 12 women followed up from 82 men and 100 women surveyed in 1994. Mean ages in the 1991/4 and 1998 studies were 53.6y(range 42-63y) and 59.0y(range 46-72y) respectively. The survey was conducted with interview using a questionnaire including dietary behaviors and health-related habits, and anthropometric measurement and blood test for analyzing biochemical indices in 1991/4 and 1998. To understand these changes over time, $\chi$$^2$-statistics, t-test and paired t-test were analyzed with an SAS package program. As people age, there were significantly decreases in the proportions to eat lightly salted foods and drink alcohol. Significant longitudinal increases in triceps skin fold thickness, ${\gamma}$-GPT and HDL-cholesterol and significant decrease in hemoglobin value were noted over time in both men and women. In addition a significant decrease in blood glucose level was noted over time in women. Weight, blood glucose and total cholesterol increased significantly with age in those had had breakfast regularly or those had not used more slat at the table in the past. From this study, were found out the subjects of this study had relatively desirable health-related habits. But as improper health-related habits are likely to result in inappropriate health status(eg, increase in WHR, triglyceride, total cholesterol and LDL-cholesterol), it is recommended that nutrition education in necessary as soon as possible to meet the health elderly.

• 한국동물학회지
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• 제39권1호
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• pp.12-20
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• 1996

K562 적혈구암 세포는 phorbol 12-myristate 13-acetate(PMA)에 의해서 대핵세포로 분화되고 gpIlla의 증가, megakaryocyte와 유사한 형태학적 변화로 특징지워진다. 또한 K562 세포는 dimethy1 sulfoxide(DMSO)나 butyrate와 같은 화학적 유도원에 의해 적혈구로 분화가 유도되고 동시에 헤모글로빈이 축척된다. 본 연구에서는 K562 세포에 대한 단일클론 항체를 생성하고 이를 이용하고 61 KDa의 표면항원을 동정하였다. 단클론항체 EK-2에 의해 인지되는 61 KDa의 표면항원은 sialic acide가 풍부해 당단백질로 사료되고, 그 epitope는 neuraminidase 절단과 peroxidase oxidation에 민감하며, 열처리에는 안정하다. K562 세포의 대핵세포로 분화시에는 61 KDa 표면항원의 발현은 증가하며, 적혈구로 분회시에는 그 발현이 감소한다. EK-2 단클론항체는 조혈세포의 분화 및 암화과정의 분자적 수준을 연구하기 위한 면역학적 probe로 이용 가능할 것으로 기대된다.