• 제목/요약/키워드: Change Member of Family

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대도시근교 농촌지역의 농가특성과 시설농가의 경영규모 변화 - 대구광역시 문산리를 사례로 - (The Characteristics of Farm Household in Suburban Areas Classified by the Farming Types and Changes of Facility Farm Household's Management Size - A Case Study of Munsanli, Taegu -)

  • 박양춘;우종현
    • 한국지역지리학회지
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    • 제7권1호
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    • pp.21-34
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    • 2001
  • 우리나라의 농촌에서는 산업화와 밀접히 연관되면서 시설작물의 재배가 증가해 왔고, 특히 1970년대 이후에는 대도시 주변지역에서 급격히 증가해 왔다. 개별농가의 영농유형은 농업노동력의 양보다는 질적 수준과 관련이 있으며, 노동력의 질적 수준에서는 특히 경영주의 연령이 중요하다. 경영규모는 경영주의 연령과 관련이 있으며 미작 재배농가보다는 시설작물 재배농가의 경영주 평균연령이 더 젊고 경영규모가 더 크다. 시설작물 재배의 주목적은 영농을 통한 상업성의 제고가 가장 원인이지만 부족한 노동력 때문에 경영규모에는 한계가 있고 특히 농산물 가격의 불안정과 수익성 악화는 적극적인 경영규모 확대를 제한하고 있다. 시설작물 재배농가의 경영규모 확대는 농지의 매입보다 임차지의 확대를 통해 이루어지며, 이때 영농조건이 유리한 농지를 중심으로 임대차가 발생된다. 결과적으로 대도시 근교 농촌지역의 유지와 발전을 위해서는 영농의 상업화와 수익성 제가 중요한 요인이지만 토지이용의 효율성 측면에서는 농지의 자연적 조건이 중요하게 작용된다. 그러나 농외부문의 취업기반이나 교육시설의 보완 없이는 촌락 공동체의 유지측면에서 가족 구성원의 분리문제와 농업노동력 부족문제가 해결될 가능성이 낮다.

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SIRT7 Exhibits Oncogenic Potential in Human Ovarian Cancer Cells

  • Wang, Hong-Ling;Lu, Ren-Quan;Xie, Su-Hong;Zheng, Hui;Wen, Xue-Mei;Gao, Xiang;Guo, Lin
    • Asian Pacific Journal of Cancer Prevention
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    • 제16권8호
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    • pp.3573-3577
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    • 2015
  • Background: Sirtuin7 (SIRT7) is a type of nicotinamide adenine dinucleotide oxidized form (NAD+)-dependent deacetylase and the least understood member of the sirtuins family; it is implicated in various processes, such as aging, DNA damage repair and cell signaling transduction. There is some evidence that SIRT7 may function as a tumor trigger for human malignancy. Here, we aimed to explore the biological function of SIRT7 in ovarian carcinoma cells and its potential mechanism. Materials and Methods: Expression of SIRT7 in ovarian cancer cell lines was detected by western blotting. Transduced cell lines with SIRT7 knockdown or overexpression were constructed. Cell viability, cologenic, apoptosis-associated and motility assays were performed to elucidate the biological function of SIRT7 in ovarian cancer cells. Results: SIRT7 demonstrated a higher level in ovarian cancer cell lines compared with normal cells. On the one hand, down-regulation of SIRT7 significantly reduced ovarian cancer cell growth, repressed colony formation and increased cancer cell apoptosis; on the other hand, up-regulation promoted the migration of cancer cells. Additionally, repression of SIRT7 also induced change in apoptosis-related molecules and subunits of the NF-${\kappa}B$ family. Conclusions: In the present study, our data indicated that SIRT7 might play a role of oncogene in ovarian malignancy and be a potential therapeutic target.

치과기공사 이미지와 전공만족도가 치기공과 학생의 중도탈락의도에 미치는 영향 (Influence of dental technicians' image and major satisfaction upon dental technology students' dropout intention)

  • 권은자;김창희;김형미
    • 대한치과의료관리학회지
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    • 제10권1호
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    • pp.9-21
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    • 2022
  • This study was carried out to understand the influence of dental technicians' image and major satisfaction on dental technology students' dropout intention. A questionnaire survey was administered to 273 students in the Department of Dental Technology. Frequency analysis, independent sample t-test, one-way ANOVA, and multiple regression analysis were performed. The results are as follows: The image of dental technicians differed significantly depending on factors such as school year, personality, whether a family member is a dental technician or not, reason for choosing a department, difficulty in one's own academic life, and disciplinary life adaptation (p<0.05). The image of dental technicians was seen to be positively perceived when the respondent had an affirmative personality, or had a dental technician among family members or chose the department for its uniqueness, or found no difficulty in academic life, and adapted well to a situational or environmental change. Major satisfaction of dental technicians was seen a meaningful difference in items such as school year, personality, awareness of dental technicians, reason for choosing a department, the difficulty in own academic life, and the disciplinary life adaptation (p<0.05). Individuals with high major satisfaction and low dropout intention included those with a positive personality, who think affirmatively about dental technicians, who chose a department owing to suiting the aptitude and interest or to the uniqueness of the major. The influential factors on dropout intention included general satisfaction out of major satisfaction, perspective and self-confident image among dental technicians, and an awareness of dental technicians. The establishment of a desirable image of dental technicians and the improvement in major satisfaction are considered likely to contribute to reducing the dropout intention of dental technology students.

국민주택(전용 85 m2 이하) 아파트평면의 변화에 대한 연구 - 제도적 변화와 사회적 변화를 중심으로 - (A Study on the Change of the Apartment Unit Plan in National Housing - Focused on Institutional and Social Changes -)

  • 최권종;진정
    • 한국주거학회논문집
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    • 제26권5호
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    • pp.123-131
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    • 2015
  • The purpose of this study is to suggest an optimum guide line of the unit plan design in national housing (less than $85m^2$ of exclusive floor area in a dwelling unit). The unit plan of apartment have been improved diversely for the residents, and the family members per household have been decreased for decades comparing with the past. In contrast, the living space per household has been increased steadily, it is because of the improvement of the living standard. The unit plan have been improved from 2-bay corridor access type and 2~3 and 4-bay staircase type. In december 2005, the balcony was permitted to be used as exclusive living area without the increase of the original living area by law. So the apartment residents have been able to use more spacious area exclusively in a dwelling unit. On the other hand, the legalization on permitting balcony area into a exclusive area resulted in a distorted wide and enlarged plan only for the business profit improvement lately. The living environment has been changed differently unlike at the time of the legislation of the national housing. The family member per household has been decreased and the related law and the institution has also been changed. Moreover It has passed 40 years since the legislation of national housing ($85m^2$). So, The size of national housing should be reviewed according to the current trend in terms of the decrease of household and the utilization of balcony area.

3T3-L1 지방세포에 대한 와송 디클로로메탄 추출물의 지질 대사 개선에 관한 연구 (The Lipid Efflux Effects of Dichloromethane Extract from Orostachys japonicus in 3T3-L1 Adipocyte Cells)

  • 김수환;이형선
    • 대한임상검사과학회지
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    • 제51권4호
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    • pp.514-520
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    • 2019
  • 본 연구는 와송 유기용매 추출물중 DCM층을 이용하여 지방세포로 유도된 3T3-L1세포에 대한 지질대사 개선효과를 확인하는 연구이다. 세포독성을 확인하기 위하여 MTS-assay를 이용하여 6가지 분획의 와송 유기용매 추출물(EtOH, Hexane, DCM, EToAc, BuOH, H2O)에 대한 독성검사를 실시하여 세포에 대한 안정성을 확인하였다. 그 결과 DCM추출물이 전 농도에 걸쳐 안정성이 있음을 확인하고, 지질대사 개선효과를 확인하기 위하여 DCM층을 사용하였다. 우선 지질대사 개선효과를 확인하기 위하여 지질 배출능을 측정하였다. 지방세포로 유도된 3T3-L1세포에 대하여 DCM추출물을 처리한 후 oil-red O염색을 실시하여 지질 축적량을 평가한 결과 지질 배출능이 개선된 것을 확인할 수 있었다. 또한 지질 배출능 분석을 위하여 지질수송 단백질인 ABCA1, ABCG1의 mRNA발현을 real-time PCR로 확인한 결과 DCM추출물 처리 시 유의적인 발현 증가가 나타났음을 확인 하였다. 따라서 본 연구를 통하여 와송 DCM추출물이 3T3-L1 지방세포에 대하여 지질대사 개선효과가 있음을 확인하였다.

만성통증 환자의 통증 조절 (Chronic pain control in patients with rheumatoid arthritis)

  • 은영
    • 근관절건강학회지
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    • 제2권1호
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    • pp.17-40
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    • 1995
  • Rheumatoid arthritis is the one of the chronic diseases, one of its major symptoms is a chronic pain. Despite developing medical treatment and surgical techniques, it is suggested that to control the pain is the goal of the treatment. But pain is an inner experience and even those closest to the patient cannot truly observe its progress or share in its suffering. The National Academy of Sciences Institute of Medicine's report on Pain and Disability concluded that there is no objective measure of pain-(exactly) no pain thermometer-nor can there ever be one, because the experience of pain is inseparable from personal perception and social influence such as culture. To explore chronic pain experience is to understand the process and property of the patient's perception of pain through the response to pain, the coping with pain, and the adaptation to pain. Therefore a qualitative study was conducted in order to gain an understanding of pain experience of patients with RA in korea. I used naturalistic inquiry as a research methodology, which had 5 axioms, the first is that realities are multiple, constructed, and holistic, the second is that knower and known are interactive, inseparable, the third is only time and context bound working hypotheses(idiographic statements) are possible, the forth is all entities are in a state of mutual simultaneous shaping, so that it is impossible to distinguish causes from effects and the last is that inquiry is value-bound. Purposive sampling was conducted as a sampling. 20 subjects who experienced pain over 10 years, lived in middle-sized city and big city in Korea, and 17 women and 3 men. The subject's age was from 32 to 62 (average 48.8), all were married, living with their spouse and children, except two-one divorced and the other widow before they became ill. I collected data using In depth structured interview. I had interviews two or three times with each subject, and the interviews were conducted at each subject's home. Each interview lasted about two hours an average. A recording was taken with the consent of the subject. I used inductive data analysis-such as unitizing and categorizing. unitizing is a process of coding, whereby raw data are systematically transformed and aggregated into units. Categorizing is a process wherby previously unitized data are organized into categories that provide descriptive or inferential information about the context or setting from which the units were derived. This process is used constant comparative method. The pain controlling process is composed of behavior of pain control. The behaviors of pain control are rearranging of ADL, hiddening role conflict, balancing treatment, and changing social relation. Rearranging of ADL includes diet management, sleep management, and the adjustment of daily life activities. The subjects try to rearrange their daily activities by modified style of motions, rearranging time span & range of activities, using auxillary facilities, and getting help in order to keep on the pace of daily life. Hiddening role conflict means to reduce conflicts between sick role and their role as a family member. In this process, the subjects use two modes, one is to control the pain complaints, and the other is to internalize the value which is to stay home is good for caring her children and being a good mother. To control pain complaints is done by 'enduring', 'understanding' the other family members, or making them undersood in order to reduce pain. Balancing treatment is composed of two aspects. One is to keep the pain within the endurable level, the other is to keep in touch with medical personnel in order to get the information of treatment and emotional support. Changing social relation is made by information seeking and sharing, formation of mutual support relation, and finally simplification of social relationships. The subjects simplify their social relationships by refraining from relations with someone who makes them physically and psychologically strained. In particular the subjects are apt to avoid contact with in-laws, and the change of relation to in-laws results in lessening the family boundary. In the course of this process, they confront the crisis of family confict result in family dissolution. This crisis is related to the threat of self-existence. Findings from this study contribute to understanding the chronic pain experience. To advance this study, we should compare this result with other cases in different cultural contexts. I think to interpret these results, korean cultural background should be considered. Especially the different family concept, more broader family members and kinship network, and the traditional medical knowledge influences patients' behavior.

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Synergistic Effect of Interleukin-18 on the Expression of Lipopolysaccharide-Induced IP-10 (CXCL-10) mRNA in Mouse Peritoneal Macrophages

  • Kim, Hyo-Young;Kim, Jae-Ryong;Kim, Hee-Sun
    • Journal of Microbiology and Biotechnology
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    • 제16권10호
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    • pp.1605-1612
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    • 2006
  • Interleukin (IL)-18, a member of the family of IL-l cytokine, is one of the principal inducers of $interferon-{\gamma}(IFN-{\gamma})$ in T lymphocytes and natural killer cells. The objective of the present study was to evaluate the effect of IL-18 on the expression of chemokine IP-10 (CXCL-10) mRNA in mouse peritoneal macrophages. IL-18 had very weak direct effect or synergistic effect with IL-12 on the expression of IP-10 mRNA in C57BL/6 mouse peritoneal macrophages. However, IL-18 pretreatment was found to playa cooperative role in the expression of lipopolysaccharide (LPS)-induced IP-10 mRNA. For the expression of LPS-induced IP-10 mRNA, the synergistic effect was detected after 16 h of IL-18 pretreatment prior to LPS stimulation. The expression level of CD14 in cells stimulated with LPS was not changed by IL-18 pretreatment, and the level of $IFN-{\gamma}$ production during IL-18 pretreatment plus LPS stimulation was barely discernible ($0.36{\pm}0.31pg/ml$). Namely, the synergistic effect of IL-18 pretreatment was not related to a change of LPS receptor, CD14 expression, and the production of $IFN-{\gamma}$ by the interaction between IL-18 and LPS. The synergistic effect of IL-18 pretreatment on the expression of LPS-induced IP-10 was related to not NF-kB but AP-1 activation, and associated with the extracellular signal-regulated kinase (ERK) pathway, one of the mitogen-activated protein kinase signaling pathways. These results provide useful information that may elucidate the mechanisms underlying the effect of IL-18 on the expression of IP-10 mRNA.

병원중심 가정간호 사업의 평가 연구 -외래 관절염 환자를 대상으로 - (Effects of Hospital-based Home Care Demonstration Project on Physical and Emotional Problems and Cost - effectiveness of Patients having Arthritis)

  • 임난영;김성윤;이은옥;이인숙
    • 근관절건강학회지
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    • 제3권1호
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    • pp.4-22
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    • 1996
  • Purposes of this study were to identify a hospital-based home care model and to improve the physical, emotional and economical effectiveness of arthritic patients through medical and nursing team approach. The design in nonequivalent control group pretest-posttest design with matched samples in terms of age, sex and disease severity. Fifty two patients in each group were assigned in Seoul, Kyunggi, Kangwon and Kwangju. Before and after 6-month period of home care, level of pain, duration of morning stiffness, Richie Index, ADL, self efficacy, depression, cost expenditure were measured. Nine patients were excluded from the control group in the period of study because of denial of participation. Contents of home care provided to the experimental group include mainly distribution of prescribed drugs, 'assessment of patients' condition and side-reactions of drug. All of the information related to the home care patient were reported to the physician. On the bases of these data, the physician prescribe the specific drugs to each patient. Each patient visited the physician every 2 or 3 month for laboratory test. Patients assigned to the control group visited the outpatient clinic once a month as usual. Null hypotheses were selected because physicians concerned about the ineffective change of patients' conditions due to indirect communication with patients through nurses. Level of pain, Richie index, ADL, self-efficacy, depression, duration of morning stiffness and direct medical cost were the home care provided to them. If a family member accompany in a home care group can save 10,676 Won/month in Seoul, 34,000 Won/month in other districts. Other in-direct cost for transportation and meal can also be saved. In conclusion, those patients with low level of ADL, high level of pain and Richie index, living in the remote area definitely need the home care.

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에이코사펜타인산이 돼지난포란의 체외 성숙에 미치는 영향 (Effects of Eicosapentaenoic Acid during In Vitro Maturation of Porcine Oocytes: Hormone Synthesis and Embryonic Developmental Potential)

  • 김강식;박흠대
    • 한국동물생명공학회지
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    • 제34권3호
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    • pp.222-231
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    • 2019
  • Among fatty acid families, the polyunsaturated fatty acids were demonstrated to be mediators in various reproductive processes as precursor of steroid hormone (via cholesterol) and prostaglandins (via arachidonic acid), and in the last decade, major research was focused on the effects of omega-6 and especially omega-3 fatty acid. Eicosapentaenoic acid, the longest members of omega-3 fatty acid family, can be produced by a series of desaturation and elongation reactions from shorter member such as α-Linolenic acid. However, very few studies have provided detailed descriptions of Eicosapentaenoic acid effects and mechanisms of action in mammalian oocytes. The purpose of this study was to evaluate the effect of Eicosapentaenoic acid supplementation on in vitro maturation and developmental potential of porcine oocytes. Various concentrations of Eicosapentaenoic acid was added into in vitro maturation medium, and we evaluated the degree of cumulus expansion, nuclear maturation rate, blastocysts quality, and levels of prostaglandin E2, 17β-estradiol, progesterone in the spent medium. High doses (100 μM) of Eicosapentaenoic acid supplementation significantly inhibited cumulus expansion and oocyte nuclear maturation, and prostaglandin E2 synthesis also significantly decreased compared with other groups (p < 0.05). Supplementation of 50 μM Eicosapentaenoic acid showed higher quality blastocysts in terms of high cell numbers and low apoptosis when compared with other groups (p < 0.05), and synthesis ratio of E2/P4 also significantly increased compared with control group (p < 0.05). However, Supplementation of 100 μM Eicosapentaenoic acid showed high apoptosis when compared with other groups (p < 0.05), and synthesis ratio of 17β-estradiol/progesterone also significantly decreased compared with control group (p < 0.05). Our results indicated that supplementation with appropriate levels of Eicosapentaenoic acid beneficially affects the change of hormone synthesis for controlling oocyte maturation, leading to improved embryo quality. However, high doses of Eicosapentaenoic acid treatment results in detrimental effects.

NMR Studies on the Structure of Human Annexin I

  • Lee, Yeon-Hee;Han, Hee-yong;Oh, Jee-Young;Na, Doe-Sun;Lee, Bong-Jin
    • 한국응용약물학회:학술대회논문집
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    • 한국응용약물학회 1997년도 춘계학술대회
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    • pp.86-86
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    • 1997
  • Human annexin I is a member of annexin family of calcium dependent phospholipid binding proteins, which have been implicated in various physiological roles including phospholipase A$_2$ (PLA$_2$) inhibition, membrane fusion and calcium channel activity. In this work, the structure of N-terminally truncated human annexin I (Δ-annexin I) and its interactions with Ca$\^$2+/, ATP and cAMP were studied at atomic level by using $^1$H, $\^$15/N, $\^$l3/C NMR (nuclear magnetic resonance) spectroscopy. The effect of Ca$\^$2+/ binding on the structure of Δ-annexin I was investigated, and compared with that of Mg$\^$2+/ binding. The addition of Ca$\^$2+/ to Δ-annexin I caused some changes in the high field and low field regions of $^1$H NMR spectra. Whereas, upon addition of Mg$\^$2+/ to Δ-annexin I, almost no change could be observed. Also we found that the binding ratio of ATP to Δ-annexin I is 1. Because Δ-annexin I is a large protein with 35 kDa molecular weight, site-specific (carbonyl-$\^$l3/C, amide-$\^$15/N) labeling technique was used to determine the interaction sites of Δ-annexin I with Ca$\^$2+/ and ATP. Assignments of all the histidinyl carbonyl carbon resonances have been completed by using Δ-annexin I along with its specific 1,2-subdomain. The carbonyl carbon resonances originating from His52 and His246 of Δ-annexin I were significantly affected by Ca$\^$2+/ binding, and some Tyr and Phe resonances were also affected. The carbonyl carbon resonances originating from His52 is significantly affected by ATP binding, therefore His52 seems to be involved in the ATP binding site of Δ-annexin I.

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