• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.288.1-288.1
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• 2002

Herbal medicines are increasingly being utilized to treat a wide variety of disease processes. The aim of this study was to evaluate the ability of aqueous extract from the roots of Platycodon grandiflorum A. DC (Campanulaceae). Changkil (CK). to affect cellular response in primary cultures of rat hepatocytes to t-butyl hydroperoxide (t-BHP) induced oxidative stress and hepatotoxicity. CK-treated cells showed an increased resistance to oxidative challenge. as revealed by a higher percent of survival capacity in respect to control cells. CK added prior or simultaneously with I-BHP reduced enganced lipid peroxidation measured as production of malondialdehyde and enhnaced intracellular reduced glutathinoe depletion by t-BHP. Furhtermore. CK protected from the t-BHP-induced intracellular generation of reactive oxygen species assessde by montioting dichlorodihydrofluorescein fluorescence. it can be concluded that CK exerts an antioxidant action insice the cell. responsible for the abserved modulation of the cellular response to oxidative challenge. and CK have a marked anitioxdative and hepatoprotective potency.

• 동의생리병리학회지
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• 제19권6호
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• pp.1585-1593
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• 2005

The genetic effects of restraint stress challenge on HPA axis and the therapeutic effect of Boshimgeonbi-tang on the stress were studied with cDNA microarray analyses, RT-PCR on hypothalamus using an immobilization-stress mice as an animal model. Male CD-1 mice were restrained in a tightly fitted and ventilated vinyl holder for 2hrs once a day, and this challenge was repeated for seven· consecutive days. In the change of body weight it showed that the Boshimgeonbi-tang is effected recovery on weight loss caused by the immobilization-stress. Seven days later, total RNA was extracted from the organs of the mouse, body-labeled with $CyDye^{TM}$ fluorescence dyes and then hybridized to CDNA microarray chip. Scanning and analyzing the array slides were carried out using GenePix4000 series scanner and GenePix $Pro^{TM}$ analyzing program, respectively. The expression profiles of 109 genes out of 6000 genes on the chip were significantly modulated in hypothalamus by the immobilization stress. Energy metabolism-, lipid metabolism-, apoptosis-, stress protein, transcriptional factor, and signal transduction-related genes were transcriptionally activated whereas DNA repair-, protein biosysthesis-, and structure integrity-related genes were down-regulated in hypothalamus. The 58 genes were up-regulated by the mRNA expression folds of 1.5 to 7.9. and the 51 genes were down-regulated by 1.5 - 5.5 fold. The 11 genes among them were selected to confirm the expression profiles by RT-PCR. The mRNA expression levels of Tnfrsf1a (apoptosis), Calm2 (cell cycle), Bag3 (apoptosis), Ogg1 (DNA repair), Aatk (apoptosis), Dffa (apoptosis), Fkbp5 (protein folding) were restored to the normal one by the treatment of Boshimgeonbi-tang.

• 디지털융복합연구
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• 제20권4호
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• pp.309-319
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• 2022

선행연구에서는 정보통신기술(ICT) 근무환경의 스트레스가 조직 구성원들의 정서에 부정적인 영향을 줌으로써 업무성과를 저하시키는 것에 대한 연구가 진행 되어왔다. 그러나 현재 코로나 팬데믹 시대를 맞아서 조직 구성원들이 스마트 환경하에 재택근무하는 비율이 높아짐에 따라 긍정적 스트레스와 부정적 스트레스가 업무성과에 미치는 영향에 대한 연구의 필요성이 제기되고 있다. 이에 따라 본 연구는 정서적 사건 반응이론을 토대로 직원들이 인지하는 긍정적, 부정적 ICT 스트레스가 긍정적 감정과 부정적 감정으로 구분되는 정서적 영향을 통해 업무성과에 미치는 영향을 분석하였다. 또한 스마트워크가 업무 성과에 미치는 영향도 분석하였다. 본 설문은 리서치 전문회사에 의뢰하여 조사 하였고 Smart PLS 3.0 툴로 분석하였다. 따라서 본 연구결과를 바탕으로 스트레스가 직원들의 정서에 부정적인 영향만을 주는 것이 아니며, 목표를 달성하고자 하는 마음에서 나타나는 도전적 스트레스가 직원들의 정서에 긍정적 감정을 유발시켜 업무성과 향상에 도움이 된다는 시사점을 제시하였다.

• Journal of Animal Science and Technology
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• 제63권3호
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• pp.501-509
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• 2021

The aim of this was evaluate the efficacy of lysozyme on growth performance, nutrient digestibility, excreta microflora population, and blood profiles of weanling pigs under Escherichia coli (E. coli) challenge. A total of 30 piglets weaned at 25 days, 7.46 kg body weight, were assigned to three dietary treatments, composed of five replications, two piglets per replication, for 7 days. The dietary treatment groups were negative control (NC; without antibiotics and lysozyme), positive control (PC; NC + antibiotics), lysozyme (NC + 0.1% lysozyme). All piglets were challenged orally with 6 ml suspension, containing E. coli K88 (2 × 10⁹ CFU/mL). Dietary supplementation with lysozyme and PC resulted in no significant differences in average daily gain and gain to feed efficiency. Weanling pigs fed with E. coli challenge with lysozyme and PC treatments had significantly enhanced nutrient retentions of dry matter and energy (p < 0.05); however, there was a tendency to increase nitrogen digestibility. Furthermore, dietary inclusion of lysozyme and antibiotics treatment groups had a beneficial effect on excreta, ileal, and cecal of the fecal microbial population as decreased E. coli (p < 0.05) counts, without effects on lactobacillus counts. A significant effect were observed on a white blood cells, epinephrine and cortisol concentrations were reduced in piglets fed diets containing E. coli challenge with lysozyme and antibiotics supplementation comparison with the NC group. Therefore, the present data indicate that lysozyme in diet could ameliorate the experimental stress response induced by E. coli in piglets by decreasing intestinal E. coli, white blood cells and stress hormones and improving nutrient digestibility.

• 약학회지
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• 제46권6호
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• pp.466-471
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• 2002

Oxidative stress is considered to be associated with many diseases, such as inflammatory and cardiovascular diseases, aging and cancer. An important etiological mechanism of these diseases may be a causal relationship between the presence of oxidants and the generation of lipid hydroperoxides derived from enzymatic reactions or xenobiotic metabolism. The hydroperoxides can be decomposed to alkoxy- (ROㆍ) and peroxy- (ROOㆍ) free radicals that can oxidize other cell components, resulting in changes in enzyme activity or the generation of mediators, which can cause further cell damage. The aim of this study was to evaluate the ability of aqueous extract from the roots of Platycodon grandiflorum A. DC (Campanulaceae), Changkil (CK), to affect cellular response in primary cultures of rat hepatocytes to t-butyl hydroperoxide (t-BHP) induced oxidative stress and hepatotoxicity. CK-treated cells showed an increased resistance to oxidative challenge, as revealed by a higher percent of survival capacity in respect to control cells. CK reduced t-BHP-enhanced lipid peroxidation measured as production of malondialdehyde and enhanced intracellular reduced glutathione depletion by t-BHP. Furthermore, CK protected from the t-BHP-induced intracellular generation of reactive oxygen species assessed by monitoring dichlorodihydrofluorescein fluorescence. It can be concluded that CK exerts an antioxidant action inside the cell, responsible for the observed modulation of the cellular response to oxidative challenge, and CK have a marked antioxidative and hepatoprotective potency.

• 한국콘텐츠학회논문지
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• 제16권4호
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• pp.100-113
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• 2016

목적: 본 연구는 아동병원 간호사를 대상으로 직무특성, 직무스트레스, 이직의도 정도 및 이들 변수간의 관계를 파악하고, 이직의도에 미치는 영향 요인들을 규명하기 위한 서술적 조사연구이다. 연구의 대상은 G시에 위치한 60병상이상의 아동병원에서 근무하는 간호사 129명이며, 본 연구에 사용된 연구도구는 대상자의 일반적 특성을 묻는 12개의 문항과 직무특성 관련 16문항, 직무스트레스 36문항, 이직의도 6문항으로 총 70개 문항으로 구성된 설문지를 이용하였다. 연구자료는 SPSS 21.0 프로그램을 이용하여 분석하였으며 빈도분석 및 기술통계, t-test, ANOVA, Pearson 상관관계 분석, stepwise 방법에 의한 다중회귀분석을 이용하여 검정하였다. 본 연구의 결과는 직무특성의 하위요인은 직무도전성과 직무적합성, 직무스트레스, 급여는 이직의도에 영향을 미치는 것으로 확인되었다.

• 한국중재학회지:중재연구
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• 제21권3호
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• pp.3-20
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• 2011

An arbitrator is an impartial person chosen to decide the issue between parties engaged in a dispute. But the arbitrator appointed by a party or arbitration institution shall be impartial or independent and should disclose to the administrator any circumstances likely to give rise to justifiable doubts as to his impartiality or independence. If, at any stage during the arbitration, new circumstances arise that may give rise to such doubts, the arbitrator shall promptly disclose such circumstances to the parties and to the administrator. Upon receipt of such information from an arbitrator or a party, an party must challenge any arbitrator whenever circumstances exist that give rise to justifiable doubts as to arbitrator's impartiality or independence. Under these circumstance, there were two cases declared by the Korean Supreme Court in relation to the cancellation of the arbitration award. One arbitral case was cancelled for the reason of the having been arbitral procedure without disclosure arbitrator's impartiality, and the other case was refused to cancel the ward for the reason of the having been arbitral procedure without challenge an arbitrator. There are not, however, the standard to decide what is definitely the arbitrator's impartiality or independence and the difference on qualification between arbitrator chosen by an party and neutral arbitrator in korean arbitration law and rules. Nevertheless, korean court require arbitrator to be impartial and independent and the arbitration parties to challenge arbitrator' impartiality or independence.

• Journal of Ginseng Research
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• 제35권1호
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• pp.111-123
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• 2011

To investigate the effects of repeated immobilization-stress challenge on the the hypothalamic-pituitary-adrenal axis, the genomic transcriptome in the adrenal cortex of immobilization-stressed mouse was analyzed by using a cDNA microarray. Mice were subjected to immobilization stress for 2 h per day for 5 consecutive d. With a 4.0-fold cutoff of arbitrary criteria, the expression levels of 168 out of 41,174 genes were significantly modulated in the adrenal cortex by stress when comparing the control and experimental groups. These genes were related to apoptosis, cell cycle, immune response, inflammatory responses, and signal transduction, and thus may be used as potential targets for the development of therapeutics for chronic stress or depression. Six significant genes among these were selected for real time polymerase chain reaction analysis to confirm the change of their expression levels. The gene for phospho 1 was also further investigated because its expression showed the greatest fold-change.

• 한국축산식품학회지
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• 제34권1호
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• pp.20-25
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• 2014

This study evaluated the effects of frankfurter fat content on Listeria monocytogenes resistance to heat stress and gastric fluid, and the Caco-2 cell invasion efficiency of the pathogen. A 10-strain mixture of L. monocytogenes was inoculated on frankfurters formulated with 10%, 20%, and 30% fat content (10%: F10, 20%: F20, 30%: F30) and stored at $10^{\circ}C$ for 30 d. The samples were analyzed for L. monocytogenes resistance to heat stress and a simulated gastric fluid challenge. The total bacteria and L. monocytogenes survival rates were measured on tryptic soy agar plus 0.6% yeast extract and Palcam agar, respectively. L. monocytogenes colonies inoculated on F10, F20, and F30 samples were used for a Caco-2 cell invasion assay. In general, no obvious differences were observed between the survival rates of total bacteria and L. monocytogenes grown on different fat contents under heat stress and gastric fluid challenge. However, L. monocytogenes obtained from the F30 samples had a significantly higher Caco-2 cell invasion efficiency than those in the F10 and F20 samples (p<0.05). These results indicate that although high fat content in food may not be related to L. monocytogenes resistance to heat stress and gastric fluid, it may increase the Caco-2 cell invasion efficiency of the pathogen.

• Asian Journal for Public Opinion Research
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• 제12권1호
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• pp.1-27
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• 2024

This study investigates the interplay between transformational leadership, personality, and work stress in public organizations, focusing on public servants in Putrajaya, Kuala Lumpur. Data from 702 public servants in Putrajaya, Kuala Lumpur, out of 800 distributed questionnaires, were analyzed. Rigorous analysis employed a structural equation model (SEM) with partial least squares (PLS-SEM) methodology. Our empirical analysis challenges the conventional belief of a positive correlation between transformational leadership and work stress in public organizations, revealing no direct link between transformational leadership and increased work stress in this context. Our study highlights a significant positive correlation between personality and work stress among public servants, emphasizing the role of individual personality characteristics in shaping work-related stress levels. Finally, personality emerged as a crucial mediator in the transformational leadership-work stress relationship, indicating that transformational leadership indirectly influences work stress through its impact on personality. This underscores the importance of considering personality as a mediating factor in understanding the transformational leadership-work stress dynamic in public organizations. In summary, our research provides insights into the relationship between transformational leadership, personality, and work stress in public organizations. These findings challenge conventional assumptions, emphasize individual differences in stress levels, and underscore the mediating role of personality in addressing work stress among public servants.