• Journal of Plant Biotechnology
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• 제46권1호
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• pp.1-8
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• 2019

The main goal of this study was to determine the genetic diversity among 36 grape cultivars grown in Palestine by using ISSR-polymerase chain reaction (PCR) fingerprints. Among the tested primers, 17 produced reasonable amplification products with high intensity and pattern stability. A total of 57 DNA fragments (loci) separated by electrophoresis on agarose gels were detected and they ranged in size, from 150 to 900 bp. Out of these fragments, 55 (88%) were polymorphic and 2 (3.5%) monomorphic. Our results also revealed an average of 3.1 loci per primer. A minimum of 1 and maximum of 10 DNA fragments were obtained (S-17, #820 and #841) and (S-31) primers, respectively. Therefore, the later primer (S-31) is considered to be the most powerful primer among the tested ones. The genetic distance matrix showed an average distance range of between 0.05 and 0.76. The maximum genetic distance value of 0.76 (24% similarity) was exhibited between the (Shami and Marawi.Hamadani.Adi) as well as (Bairuti and Marawi.Hamadani.Adi) genotypes. On the other hand, the lowest genetic distance of 0.05 (95% similarity) was exhibited between (Jandali.Tawel.Mofarad and Jandali. Kurawi.Mlzlz) along with (Shami.Aswad and Shami.mtartash. mlwn) genotypes. Furthermore, the UPGMA dendrogram generally clusters the grape cultivars into eight major clusters in addition to an isolated genotype. Based on these figures, the cultivars tested in this study could be characterized by large divergence at the DNA level. This is taking the assumption that our region has a very rich and varied clonal grape genetic structure.

• Journal of Microbiology and Biotechnology
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• 제29권3호
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• pp.357-366
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• 2019

We first confirmed the involvement of MalQ (4-${\alpha}$-glucanotransferase) in Escherichia coli glycogen breakdown by both in vitro and in vivo assays. In vivo tests of the knock-out mutant, ${\Delta}malQ$, showed that glycogen slowly decreased after the stationary phase compared to the wild-type strain, indicating the involvement of MalQ in glycogen degradation. In vitro assays incubated glycogen-mimic substrate, branched cyclodextrin (maltotetraosyl-${\beta}$-CD: G4-${\beta}$-CD) and glycogen phosphorylase (GlgP)-limit dextrin with a set of variable combinations of E. coli enzymes, including GlgX (debranching enzyme), MalP (maltodextrin phosphorylase), GlgP and MalQ. In the absence of GlgP, the reaction of MalP, GlgX and MalQ on substrates produced glucose-1-P (glc-1-P) 3-fold faster than without MalQ. The results revealed that MalQ led to disproportionate G4 released from GlgP-limit dextrin to another acceptor, G4, which is phosphorylated by MalP. In contrast, in the absence of MalP, the reaction of GlgX, GlgP and MalQ resulted in a 1.6-fold increased production of glc-1-P than without MalQ. The result indicated that the G4-branch chains of GlgP-limit dextrin are released by GlgX hydrolysis, and then MalQ transfers the resultant G4 either to another branch chain or another G4 that can immediately be phosphorylated into glc-1-P by GlgP. Thus, we propose a model of two possible MalQ-involved pathways in glycogen degradation. The operon structure of MalP-defecting enterobacteria strongly supports the involvement of MalQ and GlgP as alternative pathways in glycogen degradation.

• Asian-Australasian Journal of Animal Sciences
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• 제32권4호
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• pp.467-476
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• 2019

Objective: This research was conducted to study the genetic diversity in several Indonesian cattle breeds using microsatellite markers to classify the Indonesian cattle breeds. Methods: A total of 229 DNA samples from of 10 cattle breeds were used in this study. The polymerase chain reaction process was conducted using 12 labeled primers. The size of allele was generated using the multiplex DNA fragment analysis. The POPGEN and CERVUS programs were used to obtain the observed number of alleles, effective number of alleles, observed heterozygosity value, expected heterozygosity value, allele frequency, genetic differentiation, the global heterozygote deficit among breeds, and the heterozygote deficit within the breed, gene flow, Hardy-Weinberg equilibrium, and polymorphism information content values. The MEGA program was used to generate a dendrogram that illustrates the relationship among cattle population. Bayesian clustering assignments were analyzed using STRUCTURE program. The GENETIX program was used to perform the correspondence factorial analysis (CFA). The GENALEX program was used to perform the principal coordinates analysis (PCoA) and analysis of molecular variance. The principal component analysis (PCA) was performed using adegenet package of R program. Results: A total of 862 alleles were detected in this study. The INRA23 allele 205 is a specific allele candidate for the Sumba Ongole cattle, while the allele 219 is a specific allele candidate for Ongole Grade. This study revealed a very close genetic relationship between the Ongole Grade and Sumba Ongole cattle and between the Madura and Pasundan cattle. The results from the CFA, PCoA, and PCA analysis in this study provide scientific evidence regarding the genetic relationship between Banteng and Bali cattle. According to the genetic relationship, the Pesisir cattle were classified as Bos indicus cattle. Conclusion: All identified alleles in this study were able to classify the cattle population into three clusters i.e. Bos taurus cluster (Simmental Purebred, Simmental Crossbred, and Holstein Friesian cattle); Bos indicus cluster (Sumba Ongole, Ongole Grade, Madura, Pasundan, and Pesisir cattle); and Bos javanicus cluster (Banteng and Bali cattle).

• 천문학회보
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• 제43권2호
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• pp.38.3-38.3
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• 2018

We present the results of high-resolution near-IR spectral mapping toward the Orion KL outflow. In this study, we used the Immersion Grating Infrared Spectrometer (IGRINS) on the 2.7 m Harlan J. Smith Telescope at McDonald Observatory. IGRINS's large wavelength coverage over the H & K bands and high spectral resolving power (R ~ 45,000) allowed us to detect over 35 shock-excited ro-vibrational H2 transitions and to measure directly the gas temperature and velocity of the dense outflows. In our previous study toward the H2 peak 1 region in the Orion KL outflow, we identified 31 outflow fingers from a datacube of the H2 1-0 S(1) $2.122{\mu}m$ line and constructed a three-dimensional map of the fingers. The internal extinction (${\Delta}AV$ > 10 mag) and overall angular spread of the flow argue for an ambient medium with a high density (105 cm-3). In this presentation, we show preliminary results of additional mapping toward a remarkable chain of bows (HH 205 - HH 207) farther from the ejection center, and obtain a more clear view of the shock physics of a single isolated bullet that improves on the knowledge gained from observations of the more complex peak 1 region in our earlier study.

• 정보보호학회논문지
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• 제15권2호
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• pp.53-64
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• 2005

센서 네트워크에서 기존의안전한 라우팅 방식은 고정된 싱크(sink)를 가정하였다. 그러나 실제 센서 네트워크가 활용되는 많은 분야에서 싱크는 차량에 의해 이동되는 경우가 많다. 싱크의 이동은 감지된 데이터를 전달하기 위한 라우팅 경로의 재구성, 싱크의 위치 노출, 안전한 액세스 포인트 노드의 선택과 같은 기존의 연구에서 고려하지 않은 추가적인 문제점을 발생시틴다. 본 논문에서는 양방향 해쉬 체인과 그리드(grid) 형태의 위임 노드(delegation mode)를 이용하여 위의 문제점을 해결하는 기법을 제안한다. 요청-응답 프로토콜과 이벤트유도 프로토콜로 구성된 이 기법은 싱크의 이동에 따른 라우팅 경로를 안전하게 재구성해주고, 싱크의 위치 정보가 노출되지 않도록 보호해준다. 성능면에서 제안하는 기법은 경로를 저장하여 이용하는 라우팅 방식보다 통신 횟수가 적어 자원을 절약 할 수 있다. 본 논문은 제안한 시스템의 안전성 분석과 시뮬레이션을 통한 성능평가 결과를 보여준다.

• The Plant Pathology Journal
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• 제37권2호
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• pp.87-98
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• 2021

To establish an infection, fungal pathogens must recognize diverse signals from host surfaces. The rice blast fungus, Magnaporthe oryzae, is one of the best models studying host-pathogen interactions. This fungus recognizes physical or chemical signals from the host surfaces and initiates the development of an infection structure called appressorium. Here, we found that protein MoAfo1(appressorium formation, MGG_10422) was involved in sensing signal molecules such as cutin monomers and long chain primary alcohols required for appressorium formation. The knockout mutant (ΔMoafo1) formed a few abnormal appressoria on the onion and rice sheath surfaces. However, it produced normal appressoria on the surface of rice leaves. MoAfo1 localized to the membranes of the cytoplasm and vacuole-like organelles in conidia and appressoria. Additionally, the ΔMoafo1 mutant showed defects in appressorium morphology, appressorium penetration, invasive growth, and pathogenicity. These multiple defects might be partially due to failure to respond properly to oxidative stress. These findings broaden our understanding of the fungal mechanisms at play in the recognition of the host surface during rice blast infection.

• 구강회복응용과학지
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• 제37권1호
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• pp.23-30
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• 2021

목적: 본연구의 목적은 다양한 산용액을 이용하여 지르코니아의 표면을 처리하여 표면의 양상과 골유착에 미치는 영향을 알아보는 것이다. 연구 재료 및 방법: 준비된 지르코니아 디스크에 다양한 산용액 및 광촉매 산부식을 이용하여 표면을 처리하였다. 각 시편을 SEM으로 관찰하고, 골유착을 관찰하기 위해 MC3T3E-1 세포를 이용하여 형광염색과 역전사 중합효소 연쇄반응을 통해 평가하였다. 결과: 처리한 방법에 따라 다양한 거칠기를 보였다. 불산처리군은 표면의 거칠기가 증가하였으나 약한 네트워크 구조를 가지고 있었다. 골유착능에서는 광촉매 산부식을 시행한 군에서 더 좋은 결과를 보였다(P < 0.05). 결론: 지르코니아를 광촉매 산부식방법으로 처리할 경우 다른 산처리방법에 비해 골유착능을 높이는데 효과적일 것으로 사료된다.

• 한국IT서비스학회지
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• 제20권6호
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• pp.17-43
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• 2021

Currently, various discussions are underway to establish a more advanced management system for public rental housing. Also, it calls for improvement methods for problems arising in management of the current rental housing system. In this regard, this study aims to evaluate determinants that affect user acceptance intention of blockchain technology to implement a new system that complements drawbacks of the current public rental housing system. The study uses the Unified Theory of Acceptance and Use of Technology (UTAUT) as a theoretical model; the study model utilizes three UTAUT variables -performance expectancy, social influence, and Facilitating conditions-, applies security, availability, and reliability that are perceived from blockchain technology as external parameters to facilitate understanding of user acceptance intention of blockchain technology, and evaluates whether innovativeness can regulate the influence of performance expectancy, social influence, and Facilitating conditions on user acceptance intention of blockchain technology. Based on the significant survey result where 274 IT technicians participated, the study applies structural equation modeling to explore structure of user acceptance intention in the blockchain-based management system for public rental housing. As a result, security-reliability and availability are found to factors as determinants for user acceptance intention, affecting implementation of the public rental housing management system, while innovativeness shows no significant statistical results related to regulation effect. This study has implications in that it understands characteristics of blockchain and empirically analyzes the relationship between the characteristics and acceptance intention to enable blockchain's contribution to activate the public rental housing management system in the future.

• 한국정보통신학회논문지
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• 제26권10호
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• pp.1551-1558
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• 2022

본 논문에서는 FEC (Fog/Edge Computing) 환경에서 다중 분기구조의 부분 오프로딩을 위해 모바일 장치와 에지서버로 구성된 2계층 협력 컴퓨팅 시스템을 제안한다. 제안 시스템은 다중 분기구조에 대한 재구성 선형화 기법을 적용하여 응용 서비스 처리를 분할하는 알고리즘과 모바일 장치와 에지 서버 간의 부분 오프로딩을 통한 최적의 협업 알고리즘을 포함한다. 또한 계산 오프로딩 및 CNN 계층 스케줄링을 지연시간 최소화 문제로 공식화하고 시뮬레이션을 통해 제안 시스템의 효과를 분석한다. 실험 결과 제안 알고리즘은 DAG 및 체인 토폴로지 모두에 적합하고 다양한 네트워크 조건에 잘 적응할 수 있으며, 로컬이나 에지 전용 실행과 비교하여 효율적인 작업 처리 전략 및 처리시간을 제공한다. 또한 제안 시스템은 모바일 장치에서의 응용 서비스 최적 실행을 위한 모델의 경량화 및 에지 리소스 워크로드의 효율적 분배 관련 연구에 적용 가능하다.

• Asian Journal of Innovation and Policy
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• 제10권1호
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• pp.108-131
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• 2021

The increase of international trade across countries and borders results in increased risks associated with the inflow of new pests and diseases. These risks are likely to be increased more rapidly due to climate change. Some countries implement strict regulations on imports to prevent these risks and protect biosecurity, food safety, and public health. However, the problems arise when the diseases and pests are found in a country where their economic structure largely depends on agricultural exports and cause ripple effects on other industries and ecosystems. Therefore, establishing an effective quarantine system is essential to protect and recover from the damage caused by non-native diseases and pests. This study's objectives are 1) analyzing the agricultural policies relate to the quarantine system on diseases and pests in Korea, 2) evaluating the Korea plant quarantine system's value, and 3) simulating plant quarantine policy strategies. We estimated the Korean quarantine system's benefits on diseases and pests to reach these objectives. The benefits are measured with a willingness to pay from respondents surveyed by the contingent valuation method (CVM). The CVM approach directly asks people how much they would willingly pay for food security. Finally, the Korean quarantine system's values are simulated with several policy scenarios and different scales of infection at the regional level. The results of this study can deliver policy implications on the quarantine system innovation in developing countries including Asia.