• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2005.05a
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• pp.129-129
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• 2005

• International Journal of Industrial Entomology and Biomaterials
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• v.30 no.2
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• pp.40-44
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• 2015

To investigate whether Serratia marcescens (Enterobacteriales: Enterobacteriaceae) isolated from Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae) acts as an opportunistic bacterium in peroral infection, the primary entomopathogenic bacteria Bacillus thuringiensis (Bacillales: Bacillaceae) and Paenibacillus popilliae (Eubacteriales: Bacillaceae) were added to sawdust to perform a bioassay experiment. We found that peroral infection caused by S. marcescens could be fatal beyond a concentration of $4{\times}10^8pfu/mL$ in $2^{nd}$ stage P. b. seulensis larvae and at $6{\times}10^8pfu/mL$ in $3^{rd}$ stage P. b. seulensis larvae. In particular, mortality resulting from a combination of P. popilliae and S. marcescens was markedly increased in $2^{nd}$ stage P. b. seulensis larvae. Therefore, we confirmed that mortality was increased when S. marcescens was infected together with other entomopathogenic bacteria, and that peroral infection itself can be fatal beyond certain concentrations.

• Korean journal of applied entomology
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• v.55 no.3
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• pp.215-221
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• 2016

In this study, we used electron microscopic analysis to characterize the hemocytes in the last larva of Protaetia brevitarsis seulensis (Colbe) (Cetoniidae, Coleoptera). Granulocytes (GR), plasmatocytes (PL), oenocytoids (OE), spherulocytes (SP), prohemocytes (PR) and adipohemocytes (AD) were classified based on their size and ultrastructural differences in the circulating hemocytes. Many dark granules (<$1{\mu}m$ in diameter) in the GR's cytoplasm were observed and well-developed mitochondria, endoplasmic reticulum (ER), nucleus, and Golgi complex were also seen. After microorganisms infected, the GRs were morphologically activated and phagocytosed them. Especially, dark granules (lysosomes) were fused themselves and these bigger granules finally agglomerate together with microorganisms. Other hemocytes seem to have no immune functions.

• Journal of Sasang Constitutional Medicine
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• v.18 no.1
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• pp.22-29
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• 2006

1. Objectives and Methods The original insect of $Q\'{\i}C\'{a}o$ is described as the larvae of Holotrichia diomphalia Bates (Scarabaeidae) in oriental medicine literatures. Traditionally $Q\'{\i}C\'{a}o$ inhabit on rotting wood, compost, and organic debris of soil or thatched roofs. They have the very important distinguishing mark that wriggle along on their back through rotting wood, compost etc. and they never do inflict the living crops. but, Holotrichia diomphalia Bates as the $Q\'{\i}C\'{a}o$ is not right because of many questions, such as Holotrichia are the harmful insects eat into farm products, and those don't match well with korean farmers' moods if their cultivated lands are turned over for collecting $Q\'{\i}C\'{a}o$ larvae, and especially they don't go on using their back. 2. Results and Conclusions Accordingly, in the conclusion of this subject it is reported that the original insect of $Q\'{\i}C\'{a}o$ is the larvae of Korean Cetoniidae(Coleoptera; Scarabaeoidea) insects including Genus Protaetia Burmeister(Protaetia orientalis submarmorea Burmeister, P.brevitarsis seulensis Kolbe, etc.) and the larvae of Korean Cetoniinae insects. Also, those were the original larvae of $Q\'{\i}C\'{a}o$ that were applied to not only 'DongEuiBoGam' but also 'Dongyi Suse Bowon prescriptions'. Those feed on rotting wood, compost, and organic debris of soil or thatched roofs.

• Korean journal of applied entomology
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• v.44 no.3 s.140
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• pp.189-197
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• 2005

Cellulose-digesting bacteria were isolated from hindgut of Allomyrina dichotoma (Coleoptera: Dynastidae). The bacterial isolates were identified as Yersinia sp. and Bacillus sp. The addition of the identified bacteria to diet increased growth rate of the cetoniid beetle, Protaetia brevitarsis senlensis (Coleoptera: Cetoniidae), probably by digesting cellulose nutrient contained in the oak tree sawdust diet. An additive of wheat flour at more than 10% to the sawdust diet significantly enhanced growth of P. brevitarsis senlensis. Trimmed branches of apple trees have been disposed in the apple farms and could be used for a diet component of the cetoniid beetle when the cellulose-digesting bacteria were mixed with the derived-sawdust. Resulting manure from mass rearing of P. brevitarsis senlensis contained high organic matters and trace amounts of toxic metals. When the manure were splayed on soil, it was effective as a natural compost and significantly stimulated lettuce growth. This research suggests a model technology to use cellulose-digesting bacteria to use for culturing grub, which results in natural recycles of trimmed branches in apple farms as grub diet, and to use grub manure as a natural compost.

• International Journal of Industrial Entomology and Biomaterials
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• v.39 no.2
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• pp.60-66
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• 2019

This study was carried out to investigate the development and oviposition characteristics of Protaetia brevitarsis fed mulberry fermented sawdust added with mulberry cake. The results of rearing of P. brevitarsis larvae on oak fermented sawdust and mulberry fermented sawdust added with different ratio of mulberry cake, the development periods of larvae feed sawdust with mulberry cake were about 41.6~48.5 days and shortened by 117 or more than that without treatment. The development periods were shorter as the ratio of mulberry cake added increased and there was no difference according to the kind of sawdust. But the weight of the larvae raised in mulberry fermented sawdust added with mulberry cake was heavier than that of the larvae raised in oak fermented sawdust added with mulberry cake. When mass rearing P. brevitarsis larvae in oak fermented sawdust, the addition of 5% mulberry cake was appropriate. Also, the average number of laying eggs of female who fed diet with mulberry cake was 12.3% higher than that of untreated.

• International Journal of Industrial Entomology and Biomaterials
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• v.44 no.2
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• pp.44-54
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• 2022

The food source of white-spotted flower chafer, Protaetia brevitarsis is important to economical performance. The P. brevitarsis larva were feed by mulberry and oak fermented sawdust. The effect of additional additives, Korean black raspberry (called bokbunja) marc and blueberry marc to fermented sawdust feed on the development and oviposition characteristics were investigated. The development periods of P. brevitarsis was decreased from 165.7 d to 40.0 d (mulberry sawdust) and 169.3 d to 39.0 d (oak sawdust), but the survival rate of larva was not affected by the addition of Korean black raspberry marc to fermented sawdust. However, blueberry marc addition to the fermented sawdust did not significant effect on the development period and survival rate of P. brevitarsis. The percentage of larva weight over 2.5 g was tend to increase by addition of Korean black raspberry marc and/or blueberry marc. The number of oviposition was also tend to increase by adding additional feed to sawdust but there was no significant different.

• International Journal of Industrial Entomology and Biomaterials
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• v.30 no.2
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• pp.64-74
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• 2015

Beetles Protaetia brevitarsis seulensis Kolbe (Coleoptera: Cetoniidae) and Allomyrina dichotoma Linn. (Coleoptera: Scarabaeidae) are widely used in traditional medicine, and the number of insect-rearing farms is increasing in South Korea. The purpose of this study was to establish a multiplex PCR-based assay for rapid simultaneous detection of multiple pathogens causing insect diseases. Six insect parasites such as fungi Beauveria bassiana (Bals.-Criv.) Vuill. (Hypocreales: Cordycipitaceae) and Metarhizium anisopliae (Metschn.) Sorokin (Hypocreales: Clavicipitaceae), bacteria Bacillus thuringiensis Berliner (Bacillales: Bacillaceae), Pseudomonas aeruginosa Migula (Pseudomonadales: Pseudomonadaceae), and Serratia marcescens Bizio (Enterobacteriales: Enterobacteriaceae), and Oryctes rhinoceros nudivirus were chosen based on the severity and incidence rate of insect diseases in South Korea. Pathogen-specific primers were designed and successfully applied for simultaneous detection of multiple infectious agents in farm-bred insects P. b. seulensis and A. dichotoma using multiplex PCR and high resolution capillary electrophoresis. Our results indicate that multiplex PCR is an effective and time-saving method for simultaneous detection of multiple infections in insects, and the QIAxcel capillary electrophoresis system is useful for quantitative evaluation of the individual impact of each infectious agent on the severity of insect disease. The approach designed in this study can be utilized for rapid and accurate diagnostics of infection in insect farms.

• Korean journal of applied entomology
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• v.41 no.1
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• pp.43-47
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• 2002

This study was carried out to investigate the ecological characteristics of Protaetia orientalis submarmorea (Burmeister). The emergence period of adults is appeared from April to October, and most abundant in July∼August, once a year in the field. Hibernation took place mostly in larval stage, but some in adult stage, and were generally found in the decayed rice straws and in the soil covered with compost or similar materials with average depth of 17.6 cm The developmental characteristics in the insectary (28$^{\circ}C$, 70% RH, 16L: 8D) was investigated: the average number of eggs laid per female is 82.8, egg period 8.4 days, 1st instars of larva 10.6, 2nd 18.7, 3rd 38.1, and pupal period 35.5 days. Longevity of the adult was 135.2 days In the female and 121. 7days in the male.

• Korean journal of applied entomology
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• v.57 no.2
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• pp.97-104
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• 2018

The larva of the white-spotted flower chafer, Protaetia brevitarsis seulensis (Coleoptera: Cetoniidae), is known to have important medicinal properties, such as anti-cancer activity. However, the consumer market for the larvae is depressed because of its high production cost which needs to be reduced. This study was carried out to evaluate two spent mushroom substrates as food source for chafer larvae to improve the productivity and reduce production cost. The larvae were fed with spent substrates of king oyster mushroom (Pleurotus eryngii) (P-SMS) and shiitake mushroom (Lentinula edodes) (L-SMS), with a control of fermented oak sawdust. Effects of the diets on the development of P. brevitarsis seulensis and contents of minor nutrient components in the larvae produced were examined. In the P-SMS diet, the larval rearing period was shorter at 16.2 days, while the rate of larval weight gain and the cocoon weight were higher at 156.3% and 4.1 g, respectively, than that in the L-SMS diet. The pupation rate was 100% and the adult emergence was higher at 93.3% in the P-SMS diet than in the L-SMS diet with no difference to the control diet. In the P-SMS diet, the total nitrogen content during rearing was higher at 10.28% and the minor nutrient component, particularly Fe (145.8 mg/kg), was higher than that in other diets. The oviposition preference of adults showed no significant difference among the diets. Economic analysis showed that P-SMS costs approximately 667,960 Won less per 100 kg of larvae produced, compared to that of the control diet.