• Asian-Australasian Journal of Animal Sciences
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• 제19권9호
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• pp.1298-1304
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• 2006

The aim of this study was to provide basic haematological information to allow improved nutritional management for velvet production in spotted deer (Cervus nippon) by investigating biochemical changes in blood values during the antler growth period. Blood samples, obtained from the jugular vein of twenty-five deer, were taken every 10 days from casting (day 0) to harvesting (day 50) of velvet antler. Negligible changes were found in the concentrations of total protein, albumin, and creatinine during the antler growth period, but there were significant changes in the concentrations of urea (p<0.05) and uric acid (p<0.01). The concentration of triglyceride was significantly higher (p<0.05) during the antler growth period compared to casting time, while serum high-density lipoprotein concentrations were low and remained unchanged during the antler growth period. Serum glucose concentration increased (p<0.05) significantly and was slightly changeable during antler growth. The serum concentrations of Ca and P did not fluctuate during antler growth, while those of Na, K and Cl showed slight differences between the time of casting and the rest of the antler growth period. No significant changes in concentrations of AST, ALT, amylase, CK, GGT and LDH were detected during the antler growth period. However, the concentration of ALK-P increased during antler growth reaching its peak on day 50 after casting. We found a significant difference in the concentration of ALK-P between the time of casting and the rest of the antler growth period (p<0.01). Consequently, antler growth was associated with mild changes in measured serum biochemical values with the exception of ALK-P activity in spotted deer.

• Asian-Australasian Journal of Animal Sciences
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• 제20권10호
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• pp.1546-1550
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• 2007

This study aimed to provide basic information as the foundation for further studies on the assessment of velvet antler quality by investigating the changes in fatty acid, vitamin A and E, minerals and GAGs contents by development stage of antler in spotted deer (Cervus nippon). Twelve stags (aged 4 to 5 years) were divided into two groups and velvet antler harvested 40 days (FDG) and 60 days (SDG) after casting of the buttons from the previous set. Total saturated fatty acid was lower in FDG than SDG (p<0.05). Total monounsaturated fatty acid, conjugated linoleic acid (p<0.05), polyunsaturated fatty acid and ${\omega}$3 fatty acid were higher in FDG than SDG. The vitamin A content of FDG was higher than that of SDG, but the vitamin E content of FDG was lower than that of SDG (p<0.05). SDG had significantly higher calcium and phosphate content than FDG (p<0.05). The magnesium content showed a similar trend to the contents of calcium and phosphate, but there was no significant difference between SDG and FDG. Uronic acid content was higher in FDG than SDG but there was no significant difference. The contents of GAGs and sialic acid were significantly higher (p<0.05) in FDG than SDG. These results indicated that the longer stage of antler development had lower content of activating components and this lead to a decrease of antler quality.

• Journal of Periodontal and Implant Science
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• 제30권4호
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• pp.885-894
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• 2000

Deer antler has been widely prescribed in Chinese and Korean pharmacology. Although there have been several reports concerning the effects of deer antler, such as anti-aging action, anti-inflammatory activity, antifungal action and regulatory activity of the level of glucose, the effect on bone has not determined yet. The purpose of this study was to examine the effect of deer antler on osteoblast differentiation. Hexane extract(CN-H) and chloroform extract(CN-C) were acquired from deer antler(Cervus nippon) and MC3T3-E1 pre-osteoblasts were cultured in the presence or absence of each extract. Osteoblast differentiation was estimated with the formation of mineralized nodules and the mRNA expression of alkaline phosphatase(ALP), osteocalcin(OC) and bone sialoprotein(BSP) which are markers of osteoblast differentiation. Non-treated group did not show mineralized nodule. CN-C or CN-H-treated group showed minerlaized nodules in 16 days. In northern blot analysis, CN-C or CN-H-treated group showed the elevated expression of ALP, BSP and OC in 16 days. These results suggest the possibility to develop deer antler as a bone regenerative agent in periodontal therapy by showing the stimulating activity of deer antler on differentiation of osteoblast.

• Journal of Ecology and Environment
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• 제45권2호
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• pp.88-96
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• 2021

Background: Sika deer, Cervus nippon, were originally introduced to South Korea from Japan and Taiwan for commercial farming purposes. Unfortunately, they were released into the wild during religious events and have since begun to impact the native ecosystem and species endemic to South Korea. The study of activity patterns can improve our understanding of the environmental impact of non-native species and their association with sympatric species. Using camera traps, we studied the diel and seasonal activity patterns of non-native sika deer and quantified the temporal overlap with sympatric mammalian species in the Muljangori-oreum wetlands of Hallasan National Park, South Korea. Results: A total of 970 trap events were recorded for five mammalian species from nine locations during the camera-trap survey. Siberian roe deer (Capreolus pygargus tianschanicus) had the highest number of recorded events (72.0%), followed by sika deer (Cervus nippon) (16.2%), wild boar (Sus scrofa) (5.0%), Asian badger (Meles leucurus) (4.5%), and the Jeju weasel (Mustela sibirica quelpartis) (2.0%). Sika deer had bimodal activity patterns throughout the year, with peaks throughout the spring-autumn twilight, and day and night time throughout the winter. Relating the daily activity of sika deer with other mammalian species, roe deer expressed the highest degree of overlap (Δ₄ = 0.80) while the Asian badger demonstrated the lowest overlap (Δ₄ = 0.37). Conclusions: Our data show that sika deer are a crepuscular species with seasonal variations in daily activity patterns. Additionally, we identified the temporal differences in activity peaks between different mammals in the Muljangori-oreum wetlands and found higher degree of overlap between sika deer and roe deer during twilight hours.

• 한국환경생태학회지
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• 제30권6호
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• pp.962-969
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• 2016

본 연구는 속리산국립공원에 재도입 방사된 꽃사슴(n=6)(외래도입종)의 생태 및 행동학적 특징을 밝히기 위하여 2012년 4월부터 2016년 8월까지 수행되었다. 분석된 꽃사슴(Cervus nippon)은 GPS Collar 발신기를 부착하여 포획된 원서식지에 재 방사하였으며, 연구기간 동안 수집된 1,385개의 위치 좌표를 이용하여 연간 행동권, 고도 등 서식지 이용특성을 분석하였다. 속리산국립공원에 재도입된 꽃사슴의 연구결과, 전체 행동권은 MCP 95% $2.24{\pm}1.50km^2$(t=3.648, p<0.05), 핵심구역인 FK 50%에서 $0.46{\pm}0.31km^2$(t=3.666, p<0.05)로 나타났다. 암 수 연간 행동권은 MCP 95% 수준에서 암컷 $1.53km^2$, 수컷은 $2.94km^2$로 분석되었다. 꽃사슴의 서식지 이용 중 고도 400~500m, 경사도 $30^{\circ}{\sim}35^{\circ}$, 향 S(남향)를 평균적으로 이용하였고, 도로와의 거리는 0~100m, 수계와의 거리는 0~50m을 이용하였다. 본 연구를 통해 얻어진 자료는 외래도입종 꽃사슴에 대한 서식지 관리 정책 및 생태 등의 기초 자료로 활용될 것으로 기대된다.

• Asian-Australasian Journal of Animal Sciences
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• 제7권3호
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• pp.347-355
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• 1994

Thirty farmers, representing three major deer counties in Kyung-Kee province and 50 farmers scattered in Chung-Buk province were interviewed to determine the current status of the Korean deer industry. Items surveyed were general information, farmed deer breeds. farm facilities, feeding and nutrition, hygiene and disorder incidence, velvetting, income sources, products and their sale, management problems and herd size control. The 888 deer farmed in Kyung-Kee province included spotted deer (Cervus nippon. 69.4%), red deer (Cervus elaphus, 6.1%), wapiti (Cervus elaphus canadensis, 12.2%), spotted $\times$ red deer hybrids (6.2%), red deer $\times$ wapiti hybrids (4.6%). and fallow deer (Dama dama, 1.5%). Poor information on feeding was evidenced by wide variation among farms of daily feed supply and ratios of forage to concentrate, and poor nutritional considerations according to physiological status of deer. Hygiene needed more attention. The incidence of diseases and disorders was high: enteritis (73.3% in Kyung-Kee province), fractures (60.0%), and dystocia (56.7%). The major source of farm income was velvet antler and its blood. To improve productive efficiency, attention should be given to improving farm facilities, simplified product marketing, developing standard feeding system, and economic grazing system.

• Asian-Australasian Journal of Animal Sciences
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• 제21권9호
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• pp.1233-1237
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• 2008

The genetic variability within and among three deer species in Malaysia, namely Cervus nippon (sika), Cervus timorensis (rusa) and Cervus unicolor (sambar), were evaluated using the RAPD technique. The DNA extracted from the buffy coat of 34 sika, 38 rusa and 9 sambar were analysed using ten primers that gave bands which showed good resolution. The primers generated 164 RAPD markers in total, and these ranged in size from 150 to 900 bp. The percent of polymorphism of the bands generated per primer ranged from 66.66-93.33% for rusa, 36.84-61.14% for sambar and 52.38-100% for sika. The overall percent polymorphism observed for the 164 RAPD markers was 99.39%. The results revealed five exclusive, monomorphic markers for sambar and one exclusive, monomorphic marker for sika; none was observed for rusa. However, these cannot be declared as markers for the identification of the species without analysis of more samples, populations and species. The means of within population genetic distances, based on Dice's and Jaccard's similarity indices, were similar for the rusa (0.383 and 0.542, respectively) and sika (0.397 and 0.558, respectively) populations with the sambar population being the least variable (0.194 and 0.323, respectively). The Dice based genetic distances within the species ranged from 0.194 to 0.397 and the genetic distances among the species were 0.791-0.911. The genetic distances based on Dice's and Jaccard's similarity indices between the rusa and sambar were 0.556 and 0.713, between the rusa and sika populations were 0.552 and 0.710, and between sambar and sika were 0.622 and 0.766, respectively.

• 대한수의학회지
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• 제36권4호
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• pp.757-761
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• 1996

The animal bone-remains excavated with earthwares at Cheju Kimnyungri cave site were investigated morphologically and osteometrically. The number of bone remains were 1706 pieces by morphological criteria. Based on the classification of bony pieces excavated in the cave site, the important animal species in Cheju island during the early Tamra period (presumably between A.D.0 - A.D. 500) was as follows; wild boar(75%), deer(17%), cattle(6%), and horse in small percentage. The excavated bone remains imply that the major fauna of animal species are composed of three species, including Sus scrofa, Cervus nippon and Bos taurus. These data suggests that the archaeological remains such as bone pieces are good indicators of the fauna animals, and of zoological entity in the island.

• 한국축산식품학회지
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• 제28권3호
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• pp.276-282
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• 2008

우리나라는 전 세계 녹용의 약 80% 이상을 소비하고 있는 양록 대국이나 최근 국내 녹용시장에서의 녹용 둔갑판매 및 불법유통 현상이 문제점으로 대두되고 있다. 따라서 본 연구는 녹용의 종 감별 기술을 개발하고자 현재 국내에서 유통되고 있는 러시아산 원용, 북미산 대록, 국산화용, 중국산 깔깔이 및 알래스카산 순록 등 5종의 대표적인 녹용들을 대상으로 종간 염기서열 변이성이 매우 높은 유전자로 알려져 있는 mt DNA내 cytochrome b 및 D-loop 유전자 영역의 염기서열 분석 및 종간 변이성 비교분석을 수행하였다. 각 녹용시료에서 mt DNA를 분리하고 cytochrome b와 D-loop유전자의 특정 영역을 포함하는 primer를 설계 합성하고 PCR로 증폭한 후 DNA 증폭산물의 염기서열을 분석하여 종간 유전정보의 동일성 여부를 비교한 결과 녹용 종간에 명확한 차이를 보이는 염기서열 부위가 검출되었고 이러한 종간 염기배열 차이에 근거하여 녹용의 종 감별이 가능하였다. 또한, mt DNA cytochrome b유전자에서 종간 특이적 염기서열을 인지하는 두 종류의 제한효소(NlaIV 및 TaqI)을 이용한 PCR-RFLP 기법으로 녹용으로 인정되지 않는 순록의 종 특이적 RFLP 분자표지를 검출하였고 이를 이용하여 녹용과 순록간의 종 판별이 가능하였다. 한편, D-loop 유전자의 특정 영역 염기서열 분석기법을 이용하여 시중에서 러시아산 원용으로 유통되고 있는 녹용 절편 32개를 무작위표본 추출하여 녹용의 종 감별을 조사한 결과 러시아산 원용으로 인정되는 것은 62.5%에 불과하였고 나머지는 중국산 마록(25.0%)과 엘크 및 순록의 아종으로 추정되는 시료도 일부 검출되었다. 따라서 본 연구를 통해 사슴 녹용 mt DNA 유전자의 염기서열 유전정보 변이 차이를 이용한 염기서열 분석법과 특정 제한효소(NlaIV 및 TaqI)를 이용한 PCR-RFLP 기법은 녹용의 과학적인 종 감별과 이를 바탕으로 녹용 원산지의 추정도 가능할 것으로 기대된다.

• 환경생물
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• 제31권4호
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• pp.322-332
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• 2013

본 연구는 국내 도심지의 자연산림지와 반자연지에 방사된 꽃사슴 개체군을 모니터링 하여, 그들의 행동양식 및 서식지 특성분석을 통하여 국내 자연 환경에 적응하는 꽃사슴 (Cervus nippon taiouanus)의 개체군 조절기작을 파악하기 위해 수행되었다. 서울과 경기 주변부에 인접한 자연산림지와 반자연지에 각각 꽃사슴 12개체 (숫꽃사슴 3개체, 암꽃사슴 9개체)를 정부의 승인을 얻어 방사하였으며, 2011년 5월부터 2012년 1월까지 각 개체별로 서식지 이용 특이성을 나타낸 먹이섭취장소, 수분섭취장소, 주간 휴식장소, 야간 휴식장소 등의 서식지 유형별로 연구조사원들이 유관으로 모니터링을 하였다. 방사된 꽃사슴개체군들이 무리를 이루는 적정 성비 및 개체수는 수컷 1마리와 암컷 4~9마리이었으며, 여러 마리의 새끼들과 함께 무리를 이루는 것으로 나타났다. 꽃사슴 1마리가 소비하는 먹이 섭취량은 연평균 생초 391.62 kg이었으며, 건초는 286.90 kg이었고, 수분섭취량은 생초 섭취 시 218.28 L, 건초 섭취 시 209.89 L이었으며, 건초를 섭취하는 시기인 가을과 겨울에는 더 많은 수분을 필요로 하는 것으로 조사되었다. 본 연구 결과는 산림지와 반자연지에 방사된 꽃사슴 방사 시 꽃사슴의 성비 및 방사 개체수 결정, 방사예정지의 자원생산 능력 및 서식지 특성에 따라서 도입 할 개체수와 공간 구성 요건의 기준으로 활용가능성이 높다. 이러한 꽃사슴의 연구 결과를 통해서 국내 다른 대형 포유류의 자연서식지 방사와 관련된 모니터링 방법 및 효과적인 개체군 조절 메카니즘의 기초적 연구가 될 수 있을 것이다.