Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

The Effects of Deer(Cervus nippon) Antler Extracts on Differentiation of MC3T3 Cells

녹용 추출물에 의한 MC3T3세포의 분화 촉진

  • Yoo, Yun-Jung (Dept. of Oral Biology, College of Dentistry, Yonsei University) ;
  • Lee, Hyun-Jung (Dept. of Oral Biology, College of Dentistry, Yonsei University) ;
  • Lim, So-hyung (Dept. of Molecular Life Science-Chemical Biology) ;
  • Kang, Jung-Hwa (Dept. of Oral Biology, College of Dentistry, Yonsei University) ;
  • Lee, Eun-Hui (Dept. of Molecular Life Science-Chemical Biology) ;
  • Ohk, Seung-Ho (Dept. of Oral Biology, College of Dentistry, Yonsei University) ;
  • Choi, Bong-Kyu (Dept. of Oral Biology, College of Dentistry, Yonsei University) ;
  • Jhon, Gil-Ja (Dept. of Chemistry, Ewha Womans University)
  • 유윤정 (연세대학교 치과대학 구강생물학교실) ;
  • 이현정 (연세대학교 치과대학 구강생물학교실) ;
  • 임소형 (이화여자대학교 분자생명과학부-화학생물) ;
  • 강정화 (연세대학교 치과대학 구강생물학교실) ;
  • 이은희 (이화여자대학교 분자생명과학부-화학생물) ;
  • 옥승호 (연세대학교 치과대학 구강생물학교실) ;
  • 최봉규 (연세대학교 치과대학 구강생물학교실) ;
  • 전길자 (이화여자대학교 화학과)
  • Published : 2000.12.31
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Abstract

Deer antler has been widely prescribed in Chinese and Korean pharmacology. Although there have been several reports concerning the effects of deer antler, such as anti-aging action, anti-inflammatory activity, antifungal action and regulatory activity of the level of glucose, the effect on bone has not determined yet. The purpose of this study was to examine the effect of deer antler on osteoblast differentiation. Hexane extract(CN-H) and chloroform extract(CN-C) were acquired from deer antler(Cervus nippon) and MC3T3-E1 pre-osteoblasts were cultured in the presence or absence of each extract. Osteoblast differentiation was estimated with the formation of mineralized nodules and the mRNA expression of alkaline phosphatase(ALP), osteocalcin(OC) and bone sialoprotein(BSP) which are markers of osteoblast differentiation. Non-treated group did not show mineralized nodule. CN-C or CN-H-treated group showed minerlaized nodules in 16 days. In northern blot analysis, CN-C or CN-H-treated group showed the elevated expression of ALP, BSP and OC in 16 days. These results suggest the possibility to develop deer antler as a bone regenerative agent in periodontal therapy by showing the stimulating activity of deer antler on differentiation of osteoblast.

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