• The Korea Journal of Herbology
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• v.21 no.1
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• pp.63-69
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• 2006

Objectives : This study was designed to investigate the effects of Cervi Pantotrichum Cornu and Cervi Cornu on the growth of longitudinal bone in the adolescent male rats. Methods : Longitudinal bone growth was measured by fluorescence microscopy. To examine the effects on the growth plate metabolism, the heights of growth plate and the induction of local bone morphogenetic protein-2 were measured. Results : Treatment of Cervi Pantotrichum Cornu significantly enhanced longitudinal bone growth compared with control group. However, Cervi Cornu did not show the significant effects. Conclusion : Cervi Pantotrichum Cornu enhanced longitudinal bone growth and promoted the induction of local bone morphogenetic protein-2 of growth plate in adolescence male rats.

• The Korea Journal of Herbology
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• v.25 no.2
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• pp.1-10
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• 2010

Objectives : The present study has been undertaken to investigate the effects of Cervi Cornu on treatment of osteoporosis in ovariectomized rats. Method : In this experiment, the rats were ovariectomized. Rats were administered by Cervi Cornu. The levels of bone mineral density, osteocalcin, ALP, calcium, phosphorus in serum, calcium, phosphorus, deoxypyridinoline in urine, ash weight of bone, body weight and uterus weight were measured. Results : 1. The levels of fibula-tibial bone mineral density were significantly increased in comparison with OVX group at 8 weeks in Cervi Cornu group. 2. The levels of serum osteocalcin showed significant decrease in comparison with OVX group at 4 weeks in Cervi Cornu group. The levels of serum ALP and phosphorus showed significant decrease in comparison with OVX group at 4, 8 weeks in Cervi Cornu group group. The levels of serum calcium showed significant increase in comparison with OVX group at 4, 8 weeks in Cervi Cornu group. 3. The levels of urine calcium, phosphorus and deoxypyridinoline showed significant decrease in comparison with OVX group in Cervi Cornu group. 4. The levels of fibula-tibial ash weight showed significant increase in comparison with OVX group in Cervi Cornu group. 5. The levels of body weight were significantly decreased in comparison with OVX group at 4, 8 weeks in Cervi Cornu group. 6. The levels of uterus weight were significantly increased in comparison with OVX group in Cervi Cornu group. Conclusion : Reviewing these experimetal results, it appeared that Cervi Cornu had efficacy on treatment of osteoporosis.

• Korean Journal of Acupuncture
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• v.27 no.2
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• pp.25-34
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• 2010

Objectives : To investigate the effects of Cornu cervi parvum pharmacopuncture with regard to the blood picture and antioxidative activity in rats. Methods : Sprague-Dawley rats were divided into 3 groups (n=5 each) and were treated with Cornu cervi parvum pharmacopuncture every other day for 2 weeks. The groups are classified as follows; normal control without treatment (control group), Cornu cervi parvum pharmacopuncture at CV4 (CV4 group), and Cornu cervi parvum pharmacopuncture at BL23 (BL23 group). Thereafter, the blood and liver samples were obtained for blood analysis and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activity measurement. Results : Cornu cervi parvum pharmacopuncture groups showed higher values of red blood cell count and plasma cell volume compared with those of the control (p<0.05). However, hemoglobin level showed no significant differences among groups. With regard to the blood picture, plasma concentration in total protein and albumin showed no significant differences in pharmacopuncture groups, while higher ratio of albumin/globulin was observed in CV4 group. White blood cell counts and its composition showed no significant differences among groups. Pharmacopuncture groups showed higher values in SOD, CAT, and GSH-Px activities compared with those of control group. Conclusions : Cornu cervi parvum pharmacopuncture alleviates oxidative activities in rats.

• The Journal of Internal Korean Medicine
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• v.36 no.3
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• pp.297-307
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• 2015

Objectives: We aimed to evaluate the effect of Cervi Cornu and coenzymeQ10 on exercise and endurance capacity in rats and mice. Methods: The extract of Cervi Cornu was manufactured by the pharmacy department of Kyung Hee Oriental Medical Hospital, and CoQ10 soft cap (Ildong Pharmaceutical) was used. In total, 24 rats and 30 mice were divided into 3 groups: Control (rat=8, mouse=10), CoQ10 alone (rat=8, mouse=10), Cervi Cornu extract, and CoQ10 (rat=8, mouse=10). Ergogenic effect was evaluated by administering the Cervi Cornu extract and coenzymeQ10 to rats and measuring the time to exhaustion during treadmill running; endurance capacity was assessed by measuring cold water swimming time, serum lactate level, and serum corticosterone level in each group. At 1 week from the end of treatment, we recalculated time to exhaustion during treadmill running in rats to investigate the long-term effect of the Cervi Cornu extract and coenzymeQ10. Results: Cervi Cornu extract has long-term benefits in that it preserves the ergogenic effect caused by exercise. Cervi Cornu and coenzymeQ10 have no effect on increasing cold water swimming time in ICR mice. CoenzymeQ10 decreases the serum corticosterone level in ICR mice performing cold water swimming test. Conclusions: Cervi Cornu seems to preserve the ergogenic effect caused by exercise, but a larger study is needed to investigate effect of Cervi Cornu and coenzymeQ10 on improving endurance capacity. CoenzymeQ10 decreases serum corticosterone level and it is related with the anti-psychological fatigue effect.

• The Korea Journal of Herbology
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• v.20 no.2
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• pp.1-6
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• 2005

Objectives : The purpose of this study is to investigate genetic toxicity of Cervi pantotrichum Cornu herbal acupuncture solution(CPCHA). Methods : In this study, a series of investigation have been carried out to analyze the effects of Cervi pantotrichum Cornu herball acupuncture solution(CPCHA) on colony forming ability of NIH3T3cells, Hela cells and adrenorectal coloncell for genetic toxicity test. Results and Conclusions : From the above results, it is suggested that Cervi pantotrichum Cornu herball acupuncture solution(CPCHA) was limited 0.5-10ug/ml by test. Cervi pantotrichum Cornu herball acupuncture solution(CPCHA) did not exert the protective role to the genetic toxicity in kinds of cell lines used in this study. From these results, Cervi pantotrichum Cornu herbal aqua-acupuncture solution needs further study to prove it's function in cell culture system.

• Journal of Acupuncture Research
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• v.20 no.3
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• pp.34-44
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• 2003

Objective : Bone homeostasis is maintained by balance of bone formation and resorption. Therefore, bone related diseases arose by disturbance of this balance between osteoblast and osteoclast activities. To develop a successful screening system the therapeutic components based on oriental medicine is essential to set up systematic approach for that purpose. The purpose of this study is to the know the gene expression using cDNA microarray assay. Methods : Cervi Pantotricuhum Cornu Herbal-acupuncture extract was prepared by boiling. human osteosarcoma cells(HOS) were treated with Cervi Pantotricuhum Cornu Herbal-acupuncture solution. Then mRNA was extracted and cDNA microarray assay was performed. Results : Human osteosarcoma cells(HOS) treated with Cervi Pantotricuhum Cornu Herbal-acupuncture solution($500{\mu}g/m{\ell}$) showed that thioredoxin, TAFII31 and two novel genes were increased. However many genes decreased their expression by Cervi Pantotricuhum Cornu Herbal-acupuncture. Conclusions : This type of approach will give a good chance to explore the favorable effects of Cervi Pantotricuhum Cornu Herbal-acupuncture. Further study is needed for investigating the effect of Cervi Pantotricuhum Cornu Herbal-acupuncture.

• Journal of Pharmacopuncture
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• v.3 no.1
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• pp.177-191
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• 2000

In order to investigate the effect of the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu($BL_{23}$) and Taejo($BL_{11}$) loci on the estrogen-deficiency osteoporosis induced by ovariectomy in rats, serum osteocalcin estradiol progesterone follicular stimulating hormone(FSH) calcium(Ca) and phosphorous(P) levels were monitored. The results were as follows : 1. Serum osteocalcin levels were significantly (p<0.01, p<0.05) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 2. Serum estradiol levels were increased In the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group, but Taejo group did not show significant value. 3. Serum progesterone levels were significantly(p<0.01) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 4. Serum FSH levels were significantly(p<0.01, p<0.05) decreased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. 5. Serum Ca levels were significantly(p<0.01) increased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu group compared with control group, but Taejo group did not show significant value. 6. Serum P levels were significantly(p<0.01, p<0.05) decreased in the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo group compared with control group. According to these results, the Cervi Pantotricuhum Cornu Herb-acupuncture treated on Shinsu and Taejo loci have favorable effect on treatment and prevention of osteoporosis induced by ovariectomy in rats.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.5
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• pp.1226-1232
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• 2006

This study was carried out to investigate the effects of Cervi Pontotrichum cornu and Carthami semen on the serum osteocalcin level, bone mineral density, bone trabecular area and cortical thickness index in ovariectomized rats. Femal Sprague-Dawley rats were divided into 5 groups. Non-ovariectomized groups were divided into commertial diet fed(normal group) and calcium free basal diet fed(sham operation group). Ovariectomized groups were divided into 3groups, calcium free basal diet fed group(control group or ovariectomized group), 10% Cervi Pontotrichum cornu supplemented diet fed group and 10% Carthami semen supplemented diet fed group. After 4 weeks diet fed, serum osteocalcin level, bone mineral density, bone trabecular areas and cortical thickness index were analyzed. The bone mineral density and bone trabeculae area were significantly increased by Cervi Pontotrichum cornu and Carthami semen supplemented diet fed groups. The osteocalcin level was significantly decreased by Cervi Pontotrichum cornu and Carthami semen supplemented diet fed groups. The trabecular area(%) in epiphysis of tibia was significantly increased by Cervi Pontotrichum cornu and Carthami semen supplemented diet fed groups. The cortical thickness index in distal diaphysis of tibia was significantly increased by Cervi Pontotrichum cornu and Carthami semen supplemented diet fed groups. the alkaline phosphatase level was significantly decreased by Cervi Pontotrichum cornu and Carthami semen supplemented diet fed groups. These results suggest that Cervi Pontotrichum cornu and Carthami semen might have inhibitory effects on osteoporosis by increasing bone mineral density and accerelating bone formation in ovariectomized rats.

• Journal of Haehwa Medicine
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• v.15 no.1
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• pp.161-167
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• 2006

In oriental traditional medicine, Cervi Pantotrichium Cornu is used mainly to replenish the insufficient part of our body. Bang-Yak-Hap-Pyun(方藥合編) is a widely used book for clinical herbal prescription. In addition to replenishing what is lacking inside our body, Cervi Pantotrichium Cornu is also used in treating cardiac disease and Gynecological illness. In Bang-Yak-Hap-Pyun, there are 10 prescriptions including Cervi Pantotrichium Cornu only in sang-tong(上統) and it's portion among whole Bang-Yak-Hap-Pyun prescriptions marks 2.14%. In large, dosage of Cervi Pantotrichium Cornu in prescriptions vary 2~160g. It's ratio with other herbs vary 3.57~100%. Also, number ratio with other herb constituent vary 5.26~100%. The references are Yi-Hak-Ib-Moon(醫學入門), Dong-Yi-Bo-Gam(東醫寶鑑), Je-Joong-Sin-Pyun(濟衆新篇), etc.

• Journal of Society of Preventive Korean Medicine
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• v.4 no.2
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• pp.294-312
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• 2000

The effects of Gamisoohwabunchungum and Gamisoohwabunchungum plus Cervi Pantotrichum Cornu on rats with nephrosis induced by a single tail-intravenous injection of PAN(puromycin amjnonucleoside), 2.5mg/100g of body weight was evaluated in the present study. The effects of Gamisoohwabunchungum and Gamisoohwabunchungum plus Cervi Pantotrichum Cornu on PAN nephrosis was evaluated by measuring the concentrations of albumin, total protein, total lipid, cholesterol, triglyceride in the serum and the amount of protein in the urin. The results are summarized as follows; 1 In The Gamisoohwabunchungum and Gamisoohwabunchungum plus Cervi Pantotrichum Cornu group as compared to The Control group, The concentrations of albumin and total protein in the serum were significantly increased. 2. In The Gamisoohwabunchungum and Gamisoohwabunchungum plus Cervi Pantotrichum Cornu group as compared to The Control group, The concentrations of total lipid, cholesterol and triglyceride in the serum were significantly decreased 3. In The Gamisoohwabunchungum and Gamisoohwabunchungum plus Cervi Pantotrichum Cornu group as compared to The Control group, The amount of urine protein during 24 hours were significantly decreased . 4. In The Gamisoohwabunchungum plus Cervi Pantotrichum Cornu group as compared to The Gamisoohwabunchungum group, The concentrations of total protein in the serum were significantly increased. 5. In The Gamisoohwabunchungum plus Cervi Pantotrichum Cornu group as compared to The Gamisoohwabunchungum group, The concentrations of cholesterol and triglyceride in the serum were significantly decreased To conclude, it can be inferred that Gamisoohwabunchungum and Gamisoohwabunchungum plus Cervi Pantotrichum Cornu have the effects of improving proteinuria, hypoproteinemia, hyperlipidemia in nephrotic syndrome.