• Korean Journal of Breeding Science
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• v.43 no.1
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• pp.50-55
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• 2011

The introduction of doubled haploid (DH) approach into breeding programs has reduced the times and population sizes required for the production of pure lines. We carried out the experiment for development on effective method of producing haploid in wheat. Emasculated spikelets of wheat were pollinated with maize pollen and cultured in the solution containing 40 g/L sucrose and 2,4-D, NAA, 2,4,5-T and dicamba 24 h after pollination, and then incubated until embryo rescue. Fourteen to sixteen days after pollination, the embryos are excised and cultured in half-strength MS basal medium supplemented with 20 g/L sucrose and 1 mg/L NAA. The type of plant growth regulators was found to be most significant in production of haploid plants. The application of synthetic auxins to pollinated florets, stimulates haploid embryo development to a stage where the embryos can be rescued onto nutrient media. The percentage of seeded florets was significantly affected by 100 mg/L 2,4-D, 150 mg/L 2,4,5-T and 50 mg/L dicamba. The percentage of embryos formed was significantly increased by treatment with 2,4-D and 2,4,5-T at 100 mg/L, and dicamba at 50 mg/L, but the treatments with 150 mg/L 2,4-D inhibited embryo development and plant regeneration. The optimum application time of plant growth regulators was 24 hrs after pollination.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.4
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• pp.614-621
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• 2012

Although there is a broad dispute over genetically modified foods on safety, the worldwide adoption of transgenic crops is rapidly increasing. The objectives of this study were to identify trends in the effects of transgenic on crop yields and examine the effect of agricultural variables including crop type, biotech trait, tillage system, and yield environment on corn and soybean yield. A meta-analysis from the 34 peer-reviewed scientific literatures was conducted to compare the crop yield between transgenic crops and conventional varieties. Results showed that the yield of transgenic corn and soybean was strongly dependent on growing conditions. Transgenic hybrids had higher yield potential in the low crop yield environments such as high weeds and/or insect infestation, low soil water, and cool temperature conditions, while transgenic crops did not have yield advantages in high yield environments. The results from this study suggest that producers should consider the potential yield environmental conditions and possible yield reductions when producers choose crop hybrids in their fields.

• Proceedings of the Korean Society of Crop Science Conference
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• 2018.10a
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• pp.175-175
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• 2018

• Journal of The Korean Society of Grassland and Forage Science
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• v.34 no.2
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• pp.87-93
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• 2014

Multiple cropping system is an important agricultural system in Korea, which is significant to increase forage yield and promote agricultural economic development. This experiment was carried out to develop triple cropping systems (winter cereals crop for forage - silage corn - summer oats) for maximum year-around forage production at paddy field in southern region. We also to select a winter forage crop to determine corn planting time, and to examine the forage productivity and feed value of summer cultivated oat, which was planted after corn cultivation. When winter cereal crops for forages are harvested in accordance with and corn planter, the fresh yield and dry matter yield of rye were 32.0 ton/ha and 5.8 ton/ha, respectively, showing higher yielding compared to other winter crops. Corn silage as summer crop was showed the fresh yield and dry matter yield of 72.1 ton/ha and 20.2 ton/ha, respectively. In the feed value, crude protein and TDN contents were 7.4% and 69.3%, respectively. They was showed higher productivity and feed value. The heading date of High-speed oat was October 10, and its fresh yield and dry matter yield were 37.6 ton/ha and 8.0 ton/ha, respectively. As a feed value, crude protein and TDN content was 11.4% and 59.1%, respectively. Therefore, triple cropping systems for the production of forage all throughout the year are possible with the introduction of summer oats in the southern region, and rye could be the most suitable winter forage crops for triple cropping systems.

• The Plant Pathology Journal
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• v.30 no.1
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• pp.25-32
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• 2014

Fusarium head blight (FHB; scab) caused mainly by Fusarium graminearum is a devastating disease of wheat and barley around the world. FHB causes yield reductions and contamination of grain with trichothecene mycotoxins such as deoxynivalenol (DON) which are a major health concern for humans and animals. The objective of this research was to develop an easy seed or seedling inoculation assay, and to compare these assays with whole plant resistance of twenty-nine Korean winter wheat cultivars to FHB. The clip-dipping assay consists of cutting off the coleoptiles apex, dipping the coleoptiles apex in conidial suspension, covering in plastic bag for 3 days, and measuring the lengths of lesions 7 days after inoculation. There were significant cultivar differences after inoculation with F. graminearum in seedling relative to the controls. Correlation coefficients between the lesion lengths of clip-dipping inoculation and FHB Type II resistance from adult plants were significant (r=0.45; P<0.05). Results from two other seedling inoculation methods, spraying and pin-point inoculation, were not correlated with adult FHB resistance. Single linear correlation was not significant between seed germination assays (soaking and soak-dry) and FHB resistance (Type I and Type II), respectively. These results showed that clip-dipping inoculation method using F. graminearum may offer a real possibility of simple, rapid, and reliable for the early screening of FHB resistance in wheat.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.55 no.3
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• pp.259-267
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• 2010

This study was performed at Rice and Winter Cereal Crops Department of NICS during 2007 and 2008 to investigate the characteristics of rice leaf emergence and to obtain basic data which can be used for rice growth simulation model by which we can forecast rice growth stage and heading date accurately under different cultivars, transplanting date, and climatic conditions. To confirm leaf emergence rate according to rice maturing ecotype, we surveyed the leaf emergence rate and heading date of Unkwangbyeo, Hwayoungbyeo and Nampyeongbyeo which are early maturing, medium maturing and medium-late maturing cultivars, respectively, according to seedling raising duration and transplanting time. When seedling duration was 15 days, the growth duration between transplanting time and completion of flag leaf emergence on main culm were 51.5~78.3 days in Unkwangbyeo, 55.3~87.9 days in Hwayoungbyeo and 58.4~98.4 days in Nampyeongbyeo, respectively. When seedling duration was 30 days, they were 50.1~75.5 days in Unkwangbyeo, 52.4~84.7 days in Hwayoungbyeo and 56.4~93.8 days in Nampyeongbyeo, respectively. As transplanting time delayed, the emerged leaf number after transplanting decreased in all rice cultivars. The cumulative temperature between transplanting time to completion of flag leaf elongation on main culm were $1,281^{\circ}C{\sim}1,650^{\circ}C$ in Unkwangbyeo, $1,344^{\circ}C{\sim}1,891^{\circ}C$ in Hwayoungbyeo and $1,454^{\circ}C{\sim}2,173^{\circ}C$ in Nampyeongbyeo, respectively. Leaf emergence rate on main culm were precisely represented by equation, y = $y_0$ + a / [1 + exp( - (x - $x_0$) / b)]^c, when we used daily mean temperature as variable.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.59 no.1
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• pp.59-65
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• 2014

As for Ethephon treatment, the heading stage is 2 days later at the concentration of 250 ppm and 500 ppm for the booting stage that when there is no treatment, 4 days later at the concentration of 1000 or more ppm but no difference for the blossoming and ripening stage. The culm length get shorter as the concentration of Ethephon is higher and the rate of culm length damaged is 37% for 1500 ppm of booting stage, which is the most effective processing, and the inferior culm length damage rate is bigger than the superior culm length damage rate. There is no difference between the number of glumous flower, culm and litter weight and the non-processing and as for the thousand grain weight, it is slightly bigger than when there is not any processing. The rate of germination is indifferent, the number of seeds get numerous regardless of the concentration of treatment and the number augments by 5% maximum for the booting stage. The number of days it takes from treatment of desiccant to the moisture content for harvesting time is respectively 15 days for seeds of 30 day-treatment, 10 days for seeds of 35 days-treatment and 5 days for seeds of 40 to 45 day-treatment. As for the harvest time after treatment of desiccant, the treatment at $30^{th}$ days and $35^{th}$ after the earing is 8 days earlier than the culture by conventional methods, 8 days earlier for the treatment at $40^{th}$ day. When the desiccant treatment is implemented, the thousand grain weight is heavier as the number of days of treatment gets later. The rate of germination gets higher as the number of days of treatment after earing gets later but there is no statistically significant difference 35 days after the earing. Yields are 37% compared to the culture by conventional methods for the treatment of 30 days after the earing, 70% compared to the culture by conventional methods for the treatment of 35 days after the earing, and 92% compared to the culture by conventional methods for the treatment of 40 days after the earing. The treatment before the physiological maturity impacts greatly upon the quality of seeds.

• Journal of agriculture & life science
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• v.46 no.6
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• pp.127-135
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• 2012

This study was conducted to investigate effects of dietary anthocyanin fortified barley (AFB) or whole crop barley (WCB) on growing performance, meat quality and blood properties in swine at late fattening phase. Swine performance was not significantly differed (p>0.05) but average daily gain and average daily feed intake tended to increase in treatments. Meat quality parameters including pH, cooking loss, shear force and meat surface color were not influenced by the addition of barley in diet. However, DPPH content of longgissmus dorsi muscle was significantly increased in WCB10 compared to control, AFB5 and WCB5 (p<0.05). FRAP content of longgissmus dorsi muslce was higher in WCB5 than the AFB (p<0.05), thereby, a tendency in FRAP was not similar to that of DPPH. Only myristic acid (C14:0) was affected, and the lowest myristic acid was found when AFB was supplied to swine. A tendency was not determined in total protein and HDL-cholesterol content, however, control, WCB10 and AFB5 had high in total protein and showed significantly low values in HDL-cholesterol. Therefore, the results indicate that barley can be considered as an ingredient in swine diet, but further investigation is necessary.

• The Plant Pathology Journal
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• v.25 no.3
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• pp.231-235
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• 2009

Viral infections and root growth were examined to elucidate the relationship between viral resistance and root growth in 26 Korean hulless barley cultivars. Viral resistance was estimated in experimental filed of Honam agricultural research institute for 3 years. Length and number of seminal and adventitious roots were examined for evaluation of root growth in both field and green-house conditions 30 days after seeding. Dominant viral infection occurred in Korean hulless barley by Barley yellow mosaic virus (BaYMV) in fields; however, susceptible cultivars were infected by either BaYMV, Barley mild mosaic virus (BaMMV) or both. Only four cultivars, including Donghanchalssalbori, Kwangwhalssalbori, Namhossalbori and Naehanssalbori, presented stable resistance to viral infections. Susceptible cultivars to viral infection in fields showed shorter seminal root length and fewer adventitious root number than resistant cultivars. Resistant cultivars showed better root growth and significant difference in adventitious root length in green house conditions. Increase in the number of seminal roots in resistant cultivars was derived from decreased damage of roots by the viral infection compared to the susceptible cultivars.

• Korean Journal of Breeding Science
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• v.43 no.3
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• pp.184-189
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• 2011

'Younghan' (Hordeum vulgare L.), a winter barley for forage use, was developed by the breeding team at the Department of Rice and Winter Cereal Crop, National Institute of Crop Science, Rural Development Administration in 2008. It was derived from a cross between $F_1$['YB3433-3B-5'/'YB3135-3B-2-3'] and 'YB3135-3B-2-3'. 'SB971305-B-B-B-4-4' line was selected for its earliness, resistance to disease and good agronomic characteristics. The promising line showed both high yield and lodging resistance in the yield trials in Iksan in 2004 to 2005, and designated as 'Iksan420'. The line was subsequently evaluated for winter hardiness, earliness, and yield at eight locations throughout Korea for two years from 2007 to 2008 and finally named as 'Younghan'. It has the growth habit of IV, erect plant type, green leaf and thick culm in diameter. Its heading date was April 24, and the maturing date was May 25 in paddy field conditions, which were one day earlier than those of the check cultivar 'Youngyang'. The cultivar 'Younghan' had better winter hardiness, and resistance to lodging and BaYMV than those of the check cultivar did. The average forage dry matter yield of 'Younghan' was approximately 12.0 MT/ha in adapted region. 'Younghan' also showed 8.5% of crude protein content, 27.2% of ADF, and 67.1% of TDN, including higher grade of silage quality for whole crop barley. This cultivar would be suitable for (area with daily minimum temperature in January) above $-8^{\circ}C$ in Korean peninsula.