• Title/Summary/Keyword: Centrifugal

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Distribution and Classification of Indoor Concentration of Microorganisms in Public Buildings (다중이용시설에서의 실내공기중 미생물 분포에 관한 연구)

  • Kim Yoon Shin;Lee Eun Gyu;Yup Moo Jong;Kim Key Young
    • Journal of Environmental Health Sciences
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    • v.28 no.1
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    • pp.85-92
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    • 2002
  • A measurement of indoor microorganism using Reuter Centrifugal Air Sampler(RCS) was undertaken during October 1991 - February 1999 and 6-Stage Cascade Air Sampler was undertaken during May 2001 - June 2001 in Seoul. Sites including book store, department store, theater, subway station, underground shopping center, hospital, office building, sports facility, and eduationa institutio were chosen to measure indoor microorganism. The results were as follows: 1. The average of total microorganism collected on the agar strip GK-A media were, in the order, subway station, hospital, underground shopping center, department store, book store, theater, sports facility, educational institution, office building in sites. The highest concentration of 711cfu/m$^3$ was found in the subway station and the lowest concentration of 44cfu/m$^3$ was found in office building. 2. The average of staphylococci collected on the agar strip GK-S media, in the order, were subway station, underground shopping center, hospital and department store, department store, theater, office building, sports facility and educational institution in sites. The highest concentration of 502cfu/m$^3$ was found in the subway station and the lowest concentration of 14cfu/m$^3$ was found in sports facility and educational institution. 3. The average of fungus collected on the agar strip GK-HS media, in the order, were underground hospital, shopping center, theater, subway station, department store, book store, sports facility, educational institution, and office building in sites. The highest concentration of 252cfu/m$^3$ was found in the hospital and the lowest concentration of 32cfu/m$^3$ was found in office building. 4. Ratio of Indoor/Outdoor, determined by site was 1.12-2.38 in total count, 1.00-2.35 in staphylococci, and 0.99-1.34 in fungus. 5. The positive results of test were 12-24% in indoor and 9-43% in outdoor. 6. By gram staining gram positive cocci were 59.9%, gram positive bacill 24.4%, gram negative bacilli 10.4%, and gram negative cocci 0.5%.

Foundation-soil-foundation Interaction of Shallow Foundations Using Geo Centrifuge: Experimental Approach (원심모형실험을 이용한 얕은 기초의 기초-지반-기초 상호작용: 실험적 접근)

  • Ngo, Linh Van;Kim, Jae-Min;Lim, Jaesung;Lee, Changho
    • Journal of the Korean Geotechnical Society
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    • v.34 no.1
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    • pp.25-35
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    • 2018
  • Geocentrifuge tests are performed to investigate the structure-soil-structure interaction of shallow foundations that have various sizes. The soil specimen is prepared by using the air-pluviation, and the dynamic responses of the foundation are monitored with separation distances between the two foundations and the embedment. During the centrifugal test, the measured ground acceleration shows a tendency to increase with the increase of the input seismic amplitude, and maximum acceleration is measured at the surface due to the ground amplification. As the separation distance between the two foundations decreases, the ratio of the response spectral acceleration (RRS) increases and the period at the peak RRS decreases due to the structure-soil-structure interaction (SSSI). The RRS of the two foundations tends to decrease when the foundations are buried in the ground at the same separation distance.

Development of Miniature Cone and Characteristics of Cone Tip Resistance in Centrifuge Model Tests (원심모형실험용 소형 콘 개발 및 콘 선단저항치 특성에 관한 연구)

  • Kim, Jae Hyun;Kim, Dong Joon;Kim, Dong Soo;Choo, Yun Wook
    • KSCE Journal of Civil and Environmental Engineering Research
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    • v.33 no.2
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    • pp.631-642
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    • 2013
  • The standard CPT(Cone Penetration Test), which can be easily performed to investigate in-situ soil engineering properties, has been widely used. CPT are also widely being utilized in centrifuge model tests. In this study, a miniature cone with 10mm diameter was developed and its applicability in the centrifuge was evaluated. The developed miniature cone was equipped with a four degree-of-freedom in-flight robot. A series of cone penetration tests was performed under four centrifuge acceleration levels. As results, the cone resistances measured at the same confining stress within shallow penetration depth were affected by the centrifugal accelerations. The critical depth was proportional to the cone diameter and relative density. Cone resistances results below the critical depth and soil parameters obtained from the laboratory tests were compared with those by previously proposed empirical relations.

Bending Vibration of Rotating Cantilever Beams (회전 외팔보의 굽힘 진동해석)

  • 유홍희
    • Transactions of the Korean Society of Mechanical Engineers
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    • v.16 no.5
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    • pp.891-898
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    • 1992
  • When catilever beams rotate about axes perpendicular to the underformed beam's longitudinal axis, their bending stiffnesses change due to the stretching caused by centrifugal inertia forces. Such phenomena result in variations of natural frequencies and mode shapes associated with constant speed rotational motions of the beams. These variations are important in many practical applications such as helicopter blades, turbomachines, and space structures. This paper presents the formulation of a set of linear equations governing the lateral motion of rotating cantilever beams. These equations can be used to provide accurate predictions of the variations of natural frequencies and mode shapes associated with constant speed rotational motions of the beams. These variations are important in many practical applications such as helicopter blades, turbomachines, and space structures. This paper presents the formulation of a set of linear equations governing the lateral motion of rotating cantilever beams. These equations can be used to provide accurate predictions of the variations of natural frequencies and mode shapes due to rotation. This technique is simpler and more consistent than other conventional techniques which are commonly used in the literature.

Involvement of Lipopolysaccharide of Bradyrhizobium japonicum in Metal Binding

  • Oh, Eun-Taex;Yun, Hyun-Shik;Heo, Tae-Ryeon;Koh, Sung-Cheol;Oh, Kye-Heon;So, Jae-Seong
    • Journal of Microbiology and Biotechnology
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    • v.12 no.2
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    • pp.296-300
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    • 2002
  • Bacterial cell surface components are the major factors responsible for pathogenesis and bioremediation. In particular, the surface of a Gram-negative bacterium cell has a variety of components compared to that of a Gram-positive cell. In our previous study, we isolated an isogenic mutant of Bradyrhizobium japonicum, which exhibited altered cell surface characteristics, including an increased hydrophobicity. Polyacrylamide gel electrophoretic analysis of the lipopolysaccharide (LPS) in the mutant demonstrated that the O-polysaccharide part was completely absent. Meanwhile, a gel permeation chromatographic analysis of the exopolysaccharide (EPS) in the mutant demonstrated that it was unaltered. Since LPSs are known to have several anion groups that interact with various cation groups and metal ions, the mutant provided an opportunity to examine the direct role of LPS in metal binding by B. japonicum. Using atomic absorption spectrophotometry, it was clearly demonstrated that LPS was involved in metal binding. The binding capacity of the LPS mutant to various metal ions $(Cd^{2+},\;Cu^{2+},\;Pb^{2+},\;and\;Zn^{2+})$ was 50-70% lower than that of the wild-type strain. Also, through an EPS analysis and desorption experiment, it was found that EPS and centrifugal force had no effect on the metal binding. Accordingly, it would appear that LPS molecules on B. japonicum effect the properties, which precipitate more distinctly metal-rich mineral phase.

Proteome Analysis of various types of Panax ginseng using 2-Dimensional Electrophoresis (인삼, 산양삼 및 산삼의 부위별 Proteome분석)

  • We, Jong-Sung;Park, Hee-Soo;Kwon, Ki-Rok
    • Journal of Pharmacopuncture
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    • v.10 no.2 s.23
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    • pp.5-18
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    • 2007
  • Objectives : The purpose of this study was to obtain an objective differentiating method for various types of Panax ginseng: ginseng, cultivated wild ginseng, and natural wild ginseng which are distinctive according to their growing environment. Methods : The roots, stem, and leaves of several types of ginseng were collected and comparative analysis of proteome was conducted on each part using 2-DE and the results examined. Results : 1. Proteome images of the respective parts within the samples showed spot-matching in most cases, suggesting that they are genetically identical panax ginseng. 2. Similar distribution patters were seen within the different parts of the Panax ginseng: ginseng, Chinese cultivated wild ginseng, and the 5 and 10 years old Korean cultivated wild ginseng. 3. For a quantitative evaluation of spots showing differences among the samples, 102 spots from the roots, 109 spots from the stems, and 132 spots form the leaves which showed a difference were selected and centrifugal identification was conducted. 4. Peculiar proteins from each respective part of the Panax ginseng were identified and the top 20 spots with significant differences were selected and analyzed in order to provide a differentiation rate among the samples. The accuracy rate ranged between 23.0-38.8%. 5. Differentiation rate of the top 10 spots with significant differences showed a 50-85% accuracy rate, and the differentiation rate was especially high for the stem of Chinese cultivated wild ginseng and Korean cultivated wild ginseng.

Studies on the milling quality of major varieties of domestic and foreign produced wheat (내외국산 소맥의 주요품종별 제분성 비교연구)

  • Hi-Kap Kim
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.10
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    • pp.57-60
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    • 1971
  • In this experiment 5 Korean varieties and 3 U.S.A. varieties of wheat were investigated for their moisture content, ash content, crude protein content and extraction rate. The results are summarized as follows; 1. In the case of U.S.A. wheat, the ash content is directly proportional to the content of protein but the extraction rate is negatively correlated to the content of ash. 2. In the case of korean wheat, there is no proportional tendency as in the U.S.A. wheat. It might be cause by the low extraction rate where the endosperm is hardly separated from the bran layer at the same conditions of temering process. 3. The concentration of protein particles in the endospern, is centrifugal in the U.S.A. wheats, but in the Korean varieties the direction is oppositional to the U.S.A. varieties. 4. Yooksung #3, Cahngkwang, Youngkwang and Hard winter wheat are adapted to the production of all purpose flour or noodle flour; Noring #4, Jinkwang and Softwhite wheat are suitable for the process of cake flour; and Dark northern spring is just adaped to the production of bread flour.

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Study on Torsion due to Automotive Body Type at Track Driving (궤적주행 시 차체 종류에 따른 비틀림에 관한 연구)

  • Choi, Youn-Jong;Lee, Joon-Seong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.14 no.1
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    • pp.57-62
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    • 2013
  • Because there is no suspension and differential devices at cart body, the deformation of the frame happened during kart driving affects the driving performance caused by the elastic deformation and the fatigue life of kart frame resulted from the permanent deformation. The dynamic behavior of kart caused by the torsional deformation during circular driving is the important factor of these two kinds of deformations. In order to analyze the dynamic behavior of kart at this curved section, GPS is used to trace the track of kart and the torsional stress at kart-frame has been measured with real time. The mechanical properties of kart-frames for leisure and racing are investigated through material property analysis and tensile test. Torsional stress concentration and frame distortion are investigated through stress analysis on frame on the basis of study result. The real karts for leisure and racing kart are also tested in each driving condition by using the driving analysis equipment. The driving behavior of kart at the curved section are investigated through this test. As the phenomenon of load movement due to centrifugal force at car is happened during circular driving, the torsional stress occurs at cart steel frame.

Effect of Sulfur on the High-temperature Oxidation of Fe-34.4Cr-14.5Ni-2.5Mo-0.4W-0.4Mn-0.5Si Alloys (Fe-34.4Cr-14.5Ni-2.5Mo-0.4W-0.4Mn-0.5Si 합금의 고온 산화에 미치는 S의 영향)

  • Lee, Dong Bok;Lee, Kyong-Hwan;Bae, Geun Soo;Cho, Gyu Chul;Jung, Jae Ok;Kim, Min Jung
    • Journal of the Korean institute of surface engineering
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    • v.50 no.5
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    • pp.386-391
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    • 2017
  • Two kinds of steels whose compositions were Fe-34.4Cr-14.5Ni-2.5Mo-0.4W-0.4Mn-0.5Si-(0.009 or 0.35)S (wt.%) were centrifugally cast, and oxidized at $900^{\circ}C$ for 50-350 h in order to find the effect of sulfur on the high-temperature oxidation of Fe-34.4Cr-14.5Ni-2.5Mo-0.4W-0.4Mn-0.5Si-(0.009 or 0.35)S (wt.%) alloys. These alloys formed oxide scales that consisted primarily of $Cr_2O_3$ as the major oxide and $Cr_2MnO_4$ as the minor one through preferential oxidation of Cr and Mn. They additionally formed $SiO_2$ particles around the scale/alloy interface as well as inside the matrices. The high affinity of Mn with S led to the formation of scattered MnS inclusions particularly in the 0.35S-containing cast alloy. Sulfur was harmful to the oxidation resistance, because it deteriorated the scale/alloy adherence so as to accerelate the adherence and compactness of the formed scales.

Electronical Property of BSCCO Tube Fabricated by Centrifugal Melting Process (원심 용융 성형공정으로 제조된 BSCCO 튜브의 전기적 특성)

  • Choi, Jung-Suk;Oh, Sung-Young;Jun, Byung-Hyuk;Kim, He-Lim;Hyun, Ok-Bae;Kim, Hyoung-Seop;Kim, Chan-Joong
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.06a
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    • pp.267-267
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    • 2006
  • 전력에 큰 손실을 초래하는 고장전류를 차단하기 위한 한류기(FCL) 소재로서 고온 초전도체인 BSCCO 2212가 사용된다. 고온에서 용융된 BSCCO 2212 분말은 원심성형법에 의해 한류기용 튜브로 제조되었다. BSCCO 튜브의 기계적 특성을 높이고 용융온도를 낮추기 위해 $SrSO_4$(10wt-%)를 첨가하였다. 용탕은 $1200^{\circ}C$에서 완전히 용융되어 금속 몰드로 주입되었고 원심성형에 사용되는 금속 몰드는 $550^{\circ}C$ 온도로 2시간 예열 후 1020 ~ 2520 RPM으로 회전시켰다. 원심력에 의해 성형된 BSCCO 튜브는 약 48시간 동안 로에서 서냉 후 금속 몰드로부터 분리하였다. 튜브의 용이한 분리를 위해 이형제로서 BSCCO 2212 powder를 사용하였고 임계전류측정을 고려하여 Ag tape 단자를 튜브 끝단에 부착하였다. BSCCO 제조 공정에 있어서 몰드의 예열온도, 용융온도, 몰드 회전속도 등의 변수를 조절하여 최적의 조건을 확립하였다. 제조한 BSCCO 튜브의 임계전류($I_c$)와 임계전류밀도($J_c$)는 77K에서 536A와 $205A/cm^2$ 이었다. 본 연구에서는 BSCCO 2212 튜브를 제조하는 공정조건 변화와 각 조건에서 제조된 BSCCO 2212 튜브의 전기적 특성 및 그에 따른 분석에 대해 기술하였다.

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