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Effect of Soyabean Isoflavones Exposure on Onset of Puberty, Serum Hormone Concentration and Gene Expression in Hypothalamus, Pituitary Gland and Ovary of Female Bama Miniature Pigs

  • Fan, Juexin;Zhang, Bin;Li, Lili;Xiao, Chaowu;Oladele, Oso Abimbola;Jiang, Guoli;Ding, Hao;Wang, Shengping;Xing, Yueteng;Xiao, Dingfu;Yin, Yulong
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.11
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    • pp.1573-1582
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    • 2015
  • This study was to investigate the effect of soyabean isoflavones (SIF) on onset of puberty, serum hormone concentration, and gene expression in hypothalamus, pituitary and ovary of female Bama miniature pigs. Fifty five, 35-days old pigs were randomly assigned into 5 treatment groups consisting of 11 pigs per treatment. Results showed that dietary supplementation of varying dosage (0, 250, 500, and 1,250 mg/kg) of SIF induced puberty delay of the pigs with the age of puberty of pigs fed basal diet supplemented with 1,250 mg/kg SIF was significantly higher (p<0.05) compared to control. Supplementation of SIF or estradiol valerate (EV) reduced (p<0.05) serum gonadotrophin releasing hormone and luteinizing hormone concentration, but increased follicle-stimulating hormone concentration in pigs at 4 months of age. The expression of KiSS-1 metastasis-suppressor (KISS1), steroidogenic acute regulatory protein (StAR) and 3-beta-hydroxysteroid dehydrogenase/delta-5-delta-4 isomerase ($3{\beta}-HSD$) was reduced (p<0.01) in SIF-supplemented groups. Expression of gonadotropin-releasing hormone receptor in the pituitary of miniature pigs was reduced (p<0.05) compared to the control when exposed to 250, 1,250 mg/kg SIF and EV. Pigs on 250 mg/kg SIF and EV also showed reduced (p<0.05) expression of cytochrome P450 19A1 compared to the control. Our results indicated that dietary supplementation of SIF induced puberty delay, which may be due to down-regulation of key genes that play vital roles in the synthesis of steroid hormones.

Effects of coated cysteamine hydrochloride on muscle fiber characteristics and amino acid composition of finishing pigs

  • Bai, Miaomiao;Liu, Hongnan;Xu, Kang;Yu, Rong;Oso, Abimbola Oladele;Deng, Jinping;Yin, Yulong
    • Asian-Australasian Journal of Animal Sciences
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    • v.32 no.9
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    • pp.1430-1438
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    • 2019
  • Objective: This experiment was designed to determine the effects of coated cysteamine hydrochloride (CC) on muscle fiber characteristics, amino acid composition and transporters gene expression in the longissimus dorsi muscle (LDM) of finishing pigs. Methods: Two hundred and sixteen Duroc/Landrace/Yorkshire cross-bred male finishing pigs were fed with a corn-soybean basal diet supplemented with 0, 70, and 140 mg/kg cysteamine. Each group contained eight replicates of nine pigs per replicate. After 29 days, one pig was randomly selected from each replicate and slaughtered. Blood and LDM samples were collected and analyzed. Results: The results showed that supplemental dietary CC increased (p<0.05) the muscle fiber density. And CC supplementation also up-regulated (p<0.05) the expression of myosin heavy chain 1 (MyHC1) and MyHC2x mRNA levels, and down-regulated (p<0.05) MyHC2b expression in the LDM. Additionally, supplemental dietary CC reduced (p<0.05) the concentration of total cholesterol in the plasma and enhanced (p<0.05) the concentrations of essential amino acid and total amino acid in the LDM. The relative expression levels of chloramphenicol acetyltransferase 2, $b^{0,+}$ amino acid transporter, and $y^+$-L-type amino acid transporter 1 were upregulated (p<0.05) in the LDM when pigs were fed with the dietary CC of 70 mg/kg. Conclusion: Cysteamine supplementation could increase fiber density and distribution of fiber types. It also improved the deposition of protein in the LDM by up-regulated the expression of amino acid transporters.

Functional Ingredient and Their Some Variance in Amaranth and Quinoa (비름(Amaranth)과 명아주(Quinoa) 재배종의 기능성 물질과 변이)

  • Lee, Jae-Hak;Kim, Ki-Jun;Lee, Jung-Il;Lee, Seung-Tack;Ryu, Su-Noh
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.41 no.spc1
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    • pp.145-165
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    • 1996
  • Amaranth(Amaranthus spp. L.) and quinoa (Chenpodium quinoa Willd.) are old crops from South, Central America and Central Asia and their grains have been identified as very promising food crops because of their exceptional nutritive value. Squalene is an important ingredient in skin cosmetics and computer disc lubricants as well as bioactive materials such as inhibition of fungal and mammalian sterol biosynthesis, antitumor, anticancer, and immunomodulation. Amaranth has a component called squalene (2,6,10,15,19,23-hexamethyl-2,6,10,14,22-tetraco-sahexaene) about 1/300 of the seed and $5\~8\%$ of its seed oil. Oil and squalene content in amaranth seed were different for the species investigated. Squalene content in seed oil also increased by $15.5\%$ due to puffing and from 6.96 to $8.01\%$ by refining and bleaching. Saponin concentrations in quinoa seed ranged 0.01 to $5.6\%$. Saponins are located in the outer layers of quinoa grain. These layers include the perianth, pericarp, a seed coat layer, and a cuticle like structure. Oleanane-type triterpenes saponins are of great interest because of their diverse pharmacological properties, for instance, anti-inflammatory, antibiotic, contraceptive, and cholesterol-lowering effects. It is known that quinoa contains a number of structurally diverse saponins including the aglycones, oleanolic acid, hederagenin, and phytolaccagenic acid, which are new potential in gredient for pharmacological properties. It is likely that these saponin levels will be considerably affected by genetic, agronomic and environmental factors as well as by processing. With the current enhanced public interest in health and nutrition amaranth and quinoa will most likely remain in the immediate future within the realm of exotic health foods until such time as agricultural production meets the quantities and qualify required by industrial food manufacturers.

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Installation and Operation of a GPS Jammer Localization System (GPS 전파위협원 위치추적 시스템 구축 및 초기 운용)

  • Lim, Deok Won;Lim, Soon;Chun, Sebum;Heo, Moon Beom
    • Journal of Advanced Navigation Technology
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    • v.19 no.6
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    • pp.524-533
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    • 2015
  • In this paper, results for an installation and operation of a GPS jammer localization system were analyzed. The jammer localization system was developed by Korea Aerospace Research Institute and it consists of 4 Receiver Stations, a Central Tracking Station, and a Monitoring Station. The system was installed at Incheon International Airport in November 2014; each Receiver Stations were installed at rooftop of buildings apart from 4km, and the Central Tracking Station and a Monitoring Station were installed at indoor. Results of the operation can be monitored through web-browser in real-time, Korea Aerospace Research Institute and Incheon International Airport Corporation are continuously monitoring them. So far, there is no jamming signal which affects GPS receivers around the airport, however, some abnormal signals were frequently received at Receiver Stations. Therefore, the characteristics of those signals were also analyzed in this paper.

Accuracy Analysis of Code-based PPP-RTK Positioning Utilizing K-SSR Correction Messages Outside the Reference Network

  • Yoon, Woong-Jun;Park, Kwan-Dong;Kim, Hye-In;Woo., Seung;Park, Junpyo
    • Journal of Positioning, Navigation, and Timing
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    • v.6 no.2
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    • pp.79-86
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    • 2017
  • Precise Point Positioning-Real Time Kinematic (PPP-RTK) refers to a technology that combines PPP with network-RTK in which a user does not directly receive observed data from a reference station but receives State-Space Representation (SSR) messages corrected for error components from a central processing station through Networked Transport of RTCM via Internet Protocol (NTRIP) or Digital Multimedia Broadcasting (DMB) for purposes of positioning. SSR messages, which refer to corrections used in PPP-RTK, are generated by a central processing station using real-time observed data collected from reference stations and account for corrections needed due to the ionosphere, troposphere, satellite orbital errors, satellite time offsets, and satellite biases. This study used a type of SSR message provided in South Korea, known as Korea-SSR (K-SSR), to implement a PPP-RTK algorithm based on code-pseudorange measurements and validated its accuracy within the reference station network. In order to validate the accuracy of the implemented algorithm outside of the network, the K-SSR was extrapolated and applied to positioning in reference stations in Changchun, China (CHAN) and Japan (AIRA). This also entailed a quantitative evaluation that measured improvements in accuracy in comparison with point positioning. The results of the study showed that positioning applied with extrapolated K-SSR correction data was more accurate in both AIRA and CHAN than point positioning with improvements of approximately 20~50%.

Performance Analysis of Optical SCM Link System for CDMA RF Signal Transmission (CDMA RF 신호 전송을 위한 SCM 광링크 시스템의 성능분석)

  • 유진태
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.25 no.9B
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    • pp.1542-1550
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    • 2000
  • Fiber optic link systems based on SCM scheme are widely used as efficient and economic RF signal links between base station and a central station in mobile communication networks. However, its performance can be seriously limited depending on the operational conditions of not only the optical transmission system but also the wireless link in various environments. In this paper, we propose an analytic model for performance analyses of the SCM fiber optic link for CDMA RF signal transmission in various link environments. We present optimal operational conditions taking account of the nonlinear effects of the optical transmission system, and the multiple access interference produced at the wireless link. It has been shown through the BER analyses in this paper that the selection of optical modulation index of the SCM fiber optic links can be found optimally to minimize the fiber optic link noises and intermodulation distortion due to LD.

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Influence of forestry host plants and rearing seasons on silk gland weight of tropical tasar silkworm, Antheraea mylitta (Lepidoptera: Saturniidae) under Doon valley conditions of Uttarakhand in India

  • Bhatia, Narendra Kumar;Yousuf, Mohd.;Tewary, Pankaj;Sharma, Satya Prakash
    • International Journal of Industrial Entomology and Biomaterials
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    • v.32 no.1
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    • pp.1-11
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    • 2016
  • Tropical tasar silkworm, Antheraea mylitta is a commercial forest silkworm in India that produces tasar silk, but never experimented in Uttarakhand, a Himalayan state of India. A. mylitta express divergent phenotypic characters under different ecological conditions; so, we studied the effect of seven forest tree species in two rearing seasons on variability in silk gland weight of Daba (bivoltine) ecorace of A. mylitta at Forest Research Institute in Dehra Dun, Uttarakhand during 2012 and 2013. We used two-way completely randomized block factorial design and Post HOC Tukey's HSD test to analyse the collected data and there after carried out multiple regression analysis. Results indicated that silk gland weight differed significantly between rearing seasons (DF=1, F=2333.98, p <0.05), host plants (DF 6, F= 1516.25, p <0.05) and their interactions (DF=6, F=7.10, p <0.05). Higher silk gland weight was found in second rearing season than the first on all the host tree species. Terminalia alata fed A. mylitta larvae showed the highest silk gland weight of 8.03 and 9.47 g in first and second rearing seasons, followed by T. tomentosa (7.19 & 9.01g), T. arjuna (6.8 & 8.08 g) and L. speciosa (6.57 & 7.83 g) fed larvae, respectively. Post HOC Tukey's HSD test indicated that silk gland weight of L. speciosa and T. arjuna fed larvae in both the rearing seasons did not differ significantly. E.I. analysis also confirmed that T. alata, T. tomentosa, T. arjuna and L. speciosa are better in their order of merit than T. bellirica , T. chebula and L. tomentosa. Multiple regression analysis indicates that larval weight gain is a strong predictor (β=1.002, t=346.777, p = <0.05) for the silk gland weight of A. mylitta ; however, larval duration had significant negative regression weight (β=-0.270, t=-8.436, p = <0.05) on mean weight of silk gland.

Appropriate identification of optimum number of hidden states for identification of extreme rainfall using Hidden Markov Model: Case study in Colombo, Sri Lanka

  • Chandrasekara, S.S.K.;Kwon, Hyun-Han
    • Proceedings of the Korea Water Resources Association Conference
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    • 2019.05a
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    • pp.390-390
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    • 2019
  • Application of Hidden Markov Model (HMM) to the hydrological time series would be an innovative way to identify extreme rainfall events in a series. Even though the optimum number of hidden states can be identify based on maximizing the log-likelihood or minimizing Bayesian information criterion. However, occasionally value for the log-likelihood keep increasing with the state which gives false identification of the optimum hidden state. Therefore, this study attempts to identify optimum number of hidden states for Colombo station, Sri Lanka as fundamental approach to identify frequency and percentage of extreme rainfall events for the station. Colombo station consisted of daily rainfall values between 1961 and 2015. The representative station is located at the wet zone of Sri Lanka where the major rainfall season falls on May to September. Therefore, HMM was ran for the season of May to September between 1961 and 2015. Results showed more or less similar log-likelihood which could be identified as maximum for states between 4 to 7. Therefore, measure of central tendency (i.e. mean, median, mode, standard deviation, variance and auto-correlation) for observed and simulated daily rainfall series was carried to each state to identify optimum state which could give statistically compatible results. Further, the method was applied for the second major rainfall season (i.e. October to February) for the same station as a comparison.

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Architecture Design for Maritime Centimeter-Level GNSS Augmentation Service and Initial Experimental Results on Testbed Network

  • Kim, Gimin;Jeon, TaeHyeong;Song, Jaeyoung;Park, Sul Gee;Park, Sang Hyun
    • Journal of Positioning, Navigation, and Timing
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    • v.11 no.4
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    • pp.269-277
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    • 2022
  • In this paper, we overview the system development status of the national maritime precise point positioning-real-time kinematic (PPP-RTK) service in Korea, also known as the Precise POsitioning and INTegrity monitoring (POINT) system. The development of the POINT service began in 2020, and the open service is scheduled to start in 2025. The architecture of the POINT system is composed of three provider-side facilities-a reference station, monitoring station, and central control station-and one user-side receiver platform. Here, we propose the detailed functionality of each component considering unidirectional broadcasting of augmentation data. To meet the centimeter-level user positioning accuracy in maritime coverage, new reference stations were installed. Each reference station operates with a dual receiver and dual antenna to reduce the risk of malfunctioning, which can deteriorate the availability of the POINT service. The initial experimental results of a testbed from corrections generated from the testbed network, including newly installed reference stations, are presented. The results show that the horizontal and vertical accuracies satisfy 2.63 cm and 5.77 cm, respectively. For the purpose of (near) real-time broadcasting of POINT correction data, we designed a correction message format including satellite orbit, satellite clock, satellite signal bias, ionospheric delay, tropospheric delay, and coordinate transformation parameters. The (near) real-time experimental setup utilizing (near) real-time processing of testbed network data and the designed message format are proposed for future testing and verification of the system.

NADP-Dependent Malate Dehydrogenase Activity and Associated Biometabolic Changes in Hemolyinph and Fat Body Tissues of Silkworm Bombyx mori L. Following Baculovirus Infection

  • Krishnan, N.;Chaudhuri, A.;Sengupta, A.K.;Chandra, A.K.;Sen, S.K.;Saratchandra, B.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.2 no.2
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    • pp.149-153
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    • 2001
  • The influence of baculovirus Bombyx mori Nuclear Polyhedrosis virus (BmNPV) infection on intermediary metabolic pathways in silkworm Bombyx mori L. was investigated. Studies revealed that NADP-linked malate dehydrogenase activity in hemolymph of infected silkworms at 96 hrs post infection (p.i.) with visible symptoms of infection was enhanced in comparison to healthy larvae of the same age. Also, NADP-dependent MDH activity was significantly lower in fat body cytosol of infected larvae at 96 hrs p.i. when compared to healthy larvae. Similarly, some biometabolic parameters like growth, protein content and cholesterol titer were observed to be influenced by baculovirus infection. While the growth of infected larvae was significantly retardedi protein content was also drastically reduced in both hemolymph and fat body tissues. Cholesterol titers however, was enhanced in infected larvae. The results observed herein point to a significant change in the normal biochemical and biometabolic pathways required for growth and development following BmNPV infection.

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