Journal of the Korean Society for Precision Engineering
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v.21
no.4
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pp.132-139
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2004
The open section members have been used as the members of vehicle such as automotives, airplanes and trains. When vehicles are crashed, these members have absorption of the energy and it is necessary for retainment of the survival space, and as the result, the prediction for the displacement of members in this case of the crash of vehicles is very important. The displacements of members in this case of the crash of automotives show combined aspect of both axial collapse and bending collapse. In the rollover accident when bending collapse happen, the collapse of each members is progressed by the plastic hinge which made from bending moment, and therefore the research for the behavior of members under bending moment after collapse is necessary to determine the internal energy which the members can absorb and the deformed shapes of the members on the step of design. In this paper, the characteristics of bending collapse at the members of the open cross-section were studied with experiment and numerical analysis. We made a comparative studied of the result of the experiment, and changed the axis according to the parallel-axis theorem.
Shekastehband, Behzad;Azaraxsh, Ali A.;Showkati, Hossein
Steel and Composite Structures
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v.25
no.4
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pp.505-521
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2017
The steel plate shear wall with beam-only connected infill plate (SSW-BO) is an innovative lateral load resisting system consisting of infill plates connected to surrounding beams and separated from the main columns. In this research, the effects of perforation diameter as well as slenderness ratios of infill plates on the hysteresis behavior of SSW-BO systems were studied experimentally. Experimental testing is performed on eight one-sixth scaled one-story SSW-BO specimens with two plate thicknesses and four different circular opening ratios at the center of the panels under fully reversed cyclic quasi-static loading in compliance with the SAC test protocol. Strength, stiffness, ductility and energy absorption were evaluated based on the hysteresis loops. It is found that the systems exhibited stable hysteretic behavior during testing until significant damage in the connection of infill plates to surrounding beams at large drifts. It is also seen that pinching occurred in the hysteresis loops, since the hinge type connections were used as boundaries at four corners of surrounding frames. The strength and initial stiffness degradation of the perforated specimens containing opening ratio of 0.36 compared to the solid one is in the range of 20% to 30% and 40% to 50%, respectively.
The complete nucleotide sequence of the Bacillus circulans F-2 RSDA gene, coding for raw starch digesting a-amylase (RSDA), has been determined. The RSDA structure gene consists of an open reading frame of 2508 bp. Six bp upstream of the translational start codon of the RSDA is a typical gram-positive Shine-Dalgarno sequence and the RSDA encodes a preprotein of 836 amino acids with an Mr of 96, 727. The gene was expressed from its own regulatory region in E. coli and two putative consensus promoter sequences were identified upstream of a ribosome binding site and an ATG start codon. Confirmation of the nucleotide sequence was obtained and the signal peptide cleavage site was identified by comparing the predicted amino acid sequence with that derived by N-terminal analysis of the purified RSDA. The deduced N-terminal region of the RSDA conforms to the general pattern for the signal peptides of secreted prokaryotic proteins. The complete amino acid sequence was deduced and homology with other enzymes was compared. The results suggested that the Thr-Ser-rich hinge region and the non-catalytic domain are necessary for efficient adsorption onto raw substrates, and the catalytic domain (60 kDa) is necessary for the hydrolysis of substrates, as suggested in previous studies (8, 9).
Purpose: We wanted to evaluate the surgical results of early mobilization after rigid fixation of small coronoid fracture using the tension band technique Materials and Methods: Eight cases of coronoid fracture were fixed with the tension band technique and using K-wire and wire through the medial approach. All the cases were Regan-Morrey type 2. According to O'Driscoll, they were classified as 5 cases of the tip type (subtype 2) and 3 cases of the anteromedial type (1 case of subtype 2, and 2 case of subtype 3). The associated collateral ligament injuries (6 cases) and radial head/neck fractures (4 cases) were managed simultaneously. After immobilization for 5~7 days, active ROM exercise with a fitted hinge brace started and continued till postoperative 6 weeks. The patients were assessed for pain, ROM and functional disability using the Mayo elbow performance score (MEPS) at an average of 11 months (range: 6~28 months). The ulnar nerve symptoms were also investigated. Results: We observed solid union in all the coronoid fractures without hardware failure. An average of 2.2 wires (range: 2~4) were used. The mean extension was $3^{\circ}$(range: $0^{\circ}\sim25^{\circ}$), the mean flexion was $137^{\circ}$(range: $130^{\circ}\sim140^{\circ}$), the mean pronation was $69^{\circ}$(range: $45^{\circ}\sim90^{\circ}$) and the mean supination was $78^{\circ}$(range: $45^{\circ}\sim90^{\circ}$). The mean MEPS was 96 (range: 65~100). Ulnar nerve symptoms occurred at postoperative one day and persisted in one patient with the terrible triad of taking radial head excision and residual medial instability. Conclusion: The tension band technique uses easily obtained, economic K-wires and the wire was strong enough to permit early elbow ROM exercise and the technique might improve the elbow function. It was especially useful for fixation of multiple small fragments.
Transactions of the Korean Society of Mechanical Engineers A
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v.26
no.3
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pp.576-585
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2002
A three-dimensional Lagrangian explicit time-integration finite element code for analyzing the dynamic impact phenomena was developed. It uses four node tetrahedral elements. In order to consider the effects of strain rate hardening, strain hardening and thermal softening, which are frequently observed in high-velocity deformation phenomena, Johnson-Cook model is used as constitutive model. For more accurate and robust contact force computation, the defense node contact algorithm was adopted and implemented. In order to evaluate the performance of the newly developed three-dimensional hydrocode NET3D, numerical simulations of the oblique impact of mild steel plate by mild steel sphere were carried out. Ballistic limit about various oblique angle between 0 degree and 80 degree was estimated through a series of simulations with different initial velocities of sphere. Element eroding by equivalent plastic strain was applied to mild steel spheres and targets. Ballistic limits and fracture characteristics obtained from simulation were compared with experimental results conducted by Finnegan et al. From numerical studies, the following conclusions were reached. (1) Simulations could successfully reproduce the key features observed in experiment such as tensile failure termed "disking"at normal impacts and outwards bending of partially formed plus segments termed "hinge-mode"at oblique impacts. (2) Simulation results fur 60 degrees oblique impact at 0.70 km/s and 0.91 km/s were compared with experimental results and Eulerian hydrocode CTH simulation results. The Lagrangian code NET3D is superior to Eulerian code CTH in the computational accuracy. Agreement with the experimentally obtained final deformed cross-sections of the projectile is excellent. (3) Agreement with the experimental ballistic limit data, particularly at the high-obliquity impacts, is reasonably good. (4) The simulation result is not very sensitive to eroding condition but slightly influenced by friction coefficient.
Kim Do-Mun;Shim Young-Mog;Kim Kwhan-Mien;Choi Yong-Soo;Kim Jhin-Gook
Journal of Chest Surgery
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v.39
no.10
s.267
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pp.765-769
/
2006
Background: Nuss procedure is a minimally invasive technique for correcting the pectus excavatum. But there are some limitations of correcting the complex anomaly or grown-up patients. Material and Method: we retrospectively reviewed 135 consecutive patients who underwent repair of pectus excavatum by the Nuss procedure and its modifications between November 1999 and December 2004. We analyzed the computed tomography, age on operation, operative technique, and complications. Result: We operated 135 patients of pectus excavatum during 62 months. Total number of operations about Nuss procedure is 216, including bar removal procedure of 64 cases, redo Nuss procedures of 47 cases. We modified the point of bar insertion to the hinge point, made a shoulder in the bar to prevent a displacement. And then we changed the fixation material from Vicryl to steel wire. If the patients are old, we retract the sternum during bar rotation and fixation. Until 2002, the number of redo Nuss operations were 17, complications were 23. but, after modifications, the number of redo Nuss operation were 0, complications were 2. Conclusion: This result indicates that our modifications of Nuss operation is effective, and could decrease the number of redo Nuss operation and complications.
We have designed a 20-residue hybrid peptide CA(1-8)-MA(1-12) (CAMA) incorporating residues 1-8 of cecropin A (CA) and residues 1-12 of magainin 2 (MA) with high bacterial cell selectivity. CAMA-P2 is an ${\alpha}$-helical antimicrobial peptide designed from a CAMA hybrid peptide and substitution of Gly-Ile-Gly hinge sequence of CAMA to Pro influences the flexibility at central part of CAMA. Based on structure-activity relationships of CAMA peptides, to investigate the effects of the total positive charges on antimicrobial activity of CAMA-P2, the $Ser^{14}{\rightarrow}$Lys analogue (CAMA-syn1) was synthesized. The role of tryptophan at C-terminal ${\alpha}$-helix on its antimicrobial activity as well as synergistic activity was also investigated using $Ser^{14}{\rightarrow}$Lys/$Phe^{18}{\rightarrow}$Trp analogue (CAMA-syn2). Also, we designed CAMA-syn3 by substitution of $Lys^{16}$ located opposite side of substituted $Lys^{14}$ of CAMA-syn1 with Leu residue, resulting in increase of hydrophobicity and amphipathicity of the peptide. All of CAMA-syn analogues showed good antimicrobial activities similar to those of CAMA and CAMA-P2. The CAMA-syn1 and CAMA-syn2 showed low hemolytic activity and cytotoxicity against human keratinocyte Haca-T cells while CAMA-syn3 showed hemolytic activity and cytotoxicity at its MIC value. We then investigated their abilities to act synergistically in combination with the antimicrobial flavonoids and synthetic compounds screened in our laboratory. The results showed that all peptides exhibited synergistic effects with dihydrobinetin, while only CAMA-syn2 exhibited synergistic effects with YKAs3001 against both S. aureus and MRSA, suggesting that Trp residue at C-terminus of CAMA-syn2 may facilitate the polar antibiotic flavonoids and synthetic compounds to permeabilize the membrane. This study will be useful for the development of new antibiotic peptides with potent antimicrobial and synergistic activity but without cytotoxicity.
Putting score counts about 43 % of the golf score. The dominant idea of the putting motion to amateur golfers as well as to many professional golfers is a pendulum-like motion. If a golfer's putting stroke motion is a pendulum-like motion, the putting motion should be straight-back-and-through, the same backswing, downswing, and follow through length and period, and a swing with a fixed hinge joint. If the putting motions of the human are different from the pendulum motion, there could be confusion in understanding and teaching golf putting. The purpose of this study was to examine the center of rotation(COR) of the putter head to reveal whether professional golfers really putt like a pendulum. Thirteen male professional golfers were recruited for the study. Each golfers executed 10.94 m putts six times on an artificial grass mat. Putter head position data were collected through a 60 Hz three-dimensional motion analysis system and low pass filtered with cut-off frequency of 6 Hz. COR of the putter head was mathematically acquired. Each golfer's last five putting motions were considered. The results show that the COR of the putter head was neither fixed nor located inside of the golfer. The medio-lateral directional component of the COR of the putter head fluctuated in the range of 10 cm during downswing and follow through. The anterior-posterior directional component of the COR of the putter head was fixed from the beginning of the downswing through impact. Just after impact, however, it moved to the target up to 60 cm. The superior-inferior directional component of COR of the putter head moved in a superior direction with the beginning of the downswing and showed peak height just prior to impact. During the follow through, it moved back in an inferior direction. The height-normalized peak value of the COR of the putter head was $1.4{\pm}0.3$ height. Technically speaking, male professional golfers' 10.94 m putting motion is not a pendulum-like motion. The dominating idea of a pendulum-like motion in putting might come from the image of the flawless, smooth motion of a pendulum.
The performance of an immuno-analytical system can be assessed in terms of its analytical sensitivity, i.e., the detection limit of an analyte, which is determined by the amount of analyte molecules bound to the capture antibody that has been immobilized onto a solid surface. To increase the number of the binding complexes, we have investigated a site-directed immobilization of an antibody that has the ability to resolve a current problem associated with a random arrangement of the insolubilized immunoglobulin. The binding molecules were chemically reduced to produce thiol groups that were limited at the hinge region, and then, the reduced products were coupled to biotin. This biotinylated antibody was bound to a streptavidin-coated surface via the streptavidin-biotin reaction. This method can control the orientation of the antibody molecules present on a solid surface and also can significantly reduce the possibility of steric hindrance in the antigen-antibody reactions. In a two-site immunoassay, the introduction of the site-directly immobilized antibody as the capture enhanced the sensitivity of analyte detection approximately 10 times compared to that of the antibody randomly coupled to biotin. Such a novel approach would offer a protocol of antibody immobilization in order for the possibility of constructing a high performance immunochip.
International Journal of Control, Automation, and Systems
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v.4
no.6
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pp.748-755
/
2006
In this paper, a trade-off design and fabrication of IPMC(Ionic Polymer Metal Composites) as an actuator for a flapping device have been described. Experiments for the internal solvent loss of IPMCs have been conducted for various combinations of cation and solvent in order to find out the best combination of cation and solvent for minimal solvent loss and higher actuation force. From the experiments, it was found that IPMCs with heavy water as their solvent could operate longer. Relations between length/thickness and tip force of IPMCs were also quantitatively identified for the actuator design from the tip force measurement of 200, 400, 640, and $800{\mu}m$ thick IPMCs. All IPMCs thicker than $200{\mu}m$ were processed by casting $Nafion^{TM}$ solution. The shorter and thicker IPMCs tended to generate higher actuation force but lower actuation displacement. To improve surface conductivity and to minimize solvent evaporation due to electrically heated electrodes, gold was sputtered on both surfaces of the cast IPMCs by the Physical Vapor Deposition(PVD) process. For amplification of a short IPMC's small actuation displacement to a large flapping motion, a rack-and-pinion type hinge was used in the flapping device. An insect wing was attached to the IPMC flapping mechanism for its flapping test. In this test, the wing flapping device using the $800{\mu}m$ thick IPMC. could create around $10^{\circ}{\sim}85^{\circ}$ flapping angles and $0.5{\sim}15Hz$ flapping frequencies by applying $3{\sim|}4V$.
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